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손성한,정순일,윤문섭,김태산,박용환,김영미,Sohn, Seong-Han,Jeong, Soon-Il,Yoon, Mun-Sup,Kim, Tae-San,Park, Yong-Hwan,Kim, Young-Mi 한국응용생명화학회 2002 한국농화학회지 Vol.45 No.4
우리나라의 유전자변형농산물 의무 표시제가 시행됨에 따라 수입 유전자변형농산물 중 유전자변형 콩의 혼입유무를 판별할 수 있는 검정기술 개발이 요구되고 있다. 근사미(glyphosate)제초제에 저항성을 나타내는 토양미생물인 Agrobacterium CP4 유래의 5-enolpyruvyl shikimate-3-phosphate synthase(EPSPS) 유전자의 도입여부를 PCR로 진단할 수 있는 특이프라이머를 제작하여 제초제저항성 콩(Roundup Ready Soybean, RRS)을 검정할 수 있는 PCR조건을 확립하였으며 콩의 내재유전자인 lectin유전자와 RRS특이 프라이머를 이용하여 duplex PCR에 의한 제초제저항성 콩의 검정법을 확립하였다. 또한 수입 콩 및 콩나물에 대하여 근사미 제초제 처리로 저항성 개체를 판별하는 생물검정법도 확립하여 저항성 개체의 잎에서 분리한 genomic DNA에 대하여 EPSPS특이 프라이머를 이용하여 분석한 결과 RRS특이적인 PCR밴드를 확인하였다. 또한 수입 콩의 백립중과 종실의 제색을 고려할 때 단일품종이 아닌 여러 품종이 혼합되어 있음을 확인하였다. Along with the worldwide rapid increase of the cultivation area and commercial production of genetically modified (GM) crops, the amount of GM grains imported to Korea has also been increasing. Roundup-Ready soybean (RRS) was introduced with 5-enolpyruvyl shikimate-3-photphate synthase (EPSPS) gene derived from Agrobacterium CP4 to confer the resistance to herbicide, glyphosate. In this study, we tried to develop PCR-based analytical method to detection the presence of RRS among non-GM soybeans. In order to detect RRS specifically, oligonucleotide primers were specifically designed based on the nucleotide sequence of EPSPS transgene. Qualitative PCR method was established and its specificity and accuracy were confirmed by analysing the nucleotide sequence of PCR DNA fragments. Bioassay was also conducted by spraying glyphosate at seedling stage. Survived individuals showed obvious resistance to Roundup Ready, however all of non-GM seedlings died in two weeks after spray. Conclusively, the highly selective detection systems for RRS were successfully established by both PCR using specific primers to EPSPS transgene and bioassay using the herbicide resistance of RRS. In addition to, the imported soybean showed to be mixed to several varieties regarding to 100-seed weight and hilum color.
반하 및 차나무의 기내배양시 발생하는 세균의 동정 및 항생제 감수성 검정
김행훈,조규택,윤문섭,윤주원,조은기,Kim, Haeng-Hoon,Cho, Gyu-Taek,Yoon, Mun-Sup,Yoon, Ju-Won,Cho, Eun-Gi 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.2
Contamination of bacterial infection is one of serious problems in in vitro culture system of root crops. From the contaminated tubes over 140 of petiole cultures of Pinellia ternata, a medicinal plant, 4 genera 8species 48 strains of bacteria, including Aeromonas and Pseudomonas, were isolated and identified and another 8 strains were not fully identified. Most of them were motile Gram positive bacteria as in common in early stage of in vitro cultures. Six strains of bacteria, 5 of Gram negative, including Enterobacter, and 1 of Gram positive, were identified from the embryonic axes cultures of tea plant. From the susceptibility test to pre-screened 5 antibiotics, all of the bacteria except for 2 species of Pseudomonas were susceptible to cefotaxime 60∼100mg/L. While 60mg/L erythromycin only was effective to Pseudomonas. Combination of erythromycin 20mg/L and cefotaxime 60mg/L totally suppressed the growth of all bacterial strains tested. Susceptibility test of bacteria from tea embryonic axes cultures showed similar results. Combination of erythromycin 35mg/L and cefotaxime 60mg/L was effective to 15 bacterial strains and partially effective to 1 unidentified.
김영미 ( Young Mi Kim ),손성한 ( Seong Han Sohn ),정순일 ( Soon Il Jeong ),윤문섭 ( Mun Sup Yoon ),김태산 ( Tae San Kim ),박용환 ( Yong Hwan Park ) 한국응용생명화학회 2002 Applied Biological Chemistry (Appl Biol Chem) Vol.45 No.4
Along with the worldwide rapid increase of the cultiation area and commercial production of genetically modified (GM) crops, the amount of Gm grains imported to Korea has also been increasing. Roundup-Ready soybean (RRS) was introduced with 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) gene derived from Agrobacterium CP4 to confer the resistance to herbicide, glyphosate. In this study, we tried to develop PCR-based analytical method to detection the presence of RRS among non-GM soybeans. In order to detect RRS specifically, oligonucleotide primers were specifically designed based on the nucleotide sequence of E{S{S transgene. Qualitative PCR method was established and its specificity and accuracy were confirmed by analysing the nucleotide sequence of PCR DNA fragments. Bioassay was also conductted by spraying glyphosate at seedling stage. Survived individuals showed obvious resistance to Roundup Ready, however all of non-GM seedlings died in two weeks after spray. Conclusively, the highly selective detection systems for RRS were successfully established by both PCR using specific primers to EPSPS transgene and bioassay using the herbicide resistance of RRS. In addition to, the imported soybean showed to be mixed to several varieties regarding to 100-seed weight and hilum color.
김창영 ( Chang Yung Kim ),이정란 ( Jeong Ran Lee ),윤문섭 ( Mun Sup Yoon ),조규택 ( Gyu Taek Cho ),백형진 ( Hyung Jin Baek ),고호철 ( Ho Cheol Ko ),조양희 ( Yang Hee Cho ),전영아 ( Young Ah Jeon ),김정곤 ( Chung Kon Kim ) 한국국제농업개발학회 2010 韓國國際農業開發學會誌 Vol.22 No.3
The application and use of agricultural genetic resources have been reviewed since Korea was freed from the rule of Japanese imperialism in 1945. The whole changes that took place in the Korean agriculture, such as, characteristics of agriculture, application trends of genetic resources, roles of agricultural genebank, and future utilization perspectives of plant genetic resources were reviewed and summarized. The whole changes that took place in the Korean agriculture since the liberation from the Japanese rule were as follows. Before 1960s, Korean agriculture was a subsistence farming system with the landraces, introduction of foreign cultivars, and the beginning stage of domestic breedings. In 1960-1970s, the importance of genetic resources came up because the Korean government had to solve the national shortage of foods by developing and disseminating high-yielding cultivars such as `tongil rice`. In 1980-1990s, four-season-long cultivation of horticultural products were reared and predominantly based on the public needs. Since 2000s, diverse varieties of genetic resources became more important because of the need to cultivate crops with healthy function and high nutritional value. Hence, the interest in the diversity of agricultural genetic resources increased. The Rural Development Administration (RDA) has designated 91 local sub-banks for managing the overall national agricultural genetic resources. RDA installed the seed bank that contain more than 500,000 accessions with high technologies to preserve plant germplasms. Currently, the RDA deposits approximately 160,000 accessions of seeds, and developed and disseminated 2,477 cultivars using these genetic resources. In summary, the major role of national agricultural genebank are as follows: 1) to manage national agricultural genetic resources. 2) to secure diverse genetic resources of current and future values, to conduct multiplication and characteristic assessment of genetic resources, and to utilize genetic resources through safe preservation and distribution service. 3) to conduct a variety of researches such as genetic diversity analyses, develop seed dormancy and preservation tools, assess seed characteristics and multiplication of seeds, develop technology to discover valuable resources using functional genes. 4) to raise the national status for preservation of agricultural genetic resources through international and domestic cooperation. Agricultural genetic resources will be used for the following purposes: to continuously produce competitive cultivars with high quality, to be utilized as raw materials to meet a constant food supply and demands of the general public`s well-being, to be applied to make agri-ecosystem healthy and prosperous, and to be used as a driving force for new growth of industries related to energy, new materials and stuffs.
연구보문 : 작물재래종 유전자원의 농가보존 방안 사례조사 연구
김창영 ( Chang Yung Kim ),이정란 ( Jeong Ran Lee ),윤문섭 ( Mun Sup Yoon ),조규택 ( Gyu Taek Cho ),백형진 ( Hyung Jin Baek ),고호철 ( Ho Cheol Ko ),조양희 ( Yang Hee Cho ),전영아 ( Young Ah Jeon ),김정곤 ( Chung Kon Kim ) 한국국제농업개발학회 2011 韓國國際農業開發學會誌 Vol.23 No.3
작물재래종을 보존 재배하는 농가가 거의 없어서 재래종 자원의 소실과 멸종위기를 우려하여야 할 시점이지만, 부분적인 대응책을 마련하여 작물재래종을 지속적으로 재배 유지하는 경우가 있어 그 사례를 조사 분석하여 작물재래종의 지속적인 농가보존 방안을 강구하고자 2005년~2008년 기간 중에 사례조사 연구를 수행하였으며 그 결과를 요약하면 다음과 같다. 1. 작물재래종의 지속적인 농가재배 보존방안을 위하여 농업인단체로서 원주시 신림농협은 전통잡곡 전시포를 운영하여 토종잡곡 종자를 수집·보존·공급 기지화 및 교육장화 하는 한편, 재래종을 농가계약재배·수매하고 소포장하여 대형유통 매장 등을 통하여 판매하고 있으며, 전국여성농민회총연합은 토종씨드림 협의체를 구성하여 토종자원 실태조사, 채종포운영, 전통농업워크숍, 토종씨앗 갖기 운동 등을 전개하고 있다. 2. 법인체로서의 영월 승당가공영농조합은 잡곡전문 영농조합법인으로 국내산 잡곡 계약재배·수매·판매 사업을 하므로서 작물재래종 재배 농가를 유지하고 있으며, 강진 ``잡곡은 보약``영농조합은 주로 학교급식용 제공을 위한 국내산 전통잡곡 계약재배·수매·판매 사업을 통하여 농가의 재래종 재배가 가능토록 하고 있다. 3. 경상북도농업자원관리원(의성분원)은 작물재래종을 수집하여 보존 및 종자생산하여 농가에 종자보급을 하고, 경상남도농업자원관리원은 토종농산물 전시포 설치 및 종자은행을 운영하고 있으며, 영주시 농업기술센터는 콩 재래종 ``부석``콩 발굴 및 잡곡 프로젝트에 참여하여 재래종을 보존하고 있다. 4. 정선군 동트는 농가는 국내 재래콩인 ``쥐눈이콩``의 생산·가공 판매 사업을 추진하여 지속적으로 재래종을 보존 재배하고 있다. 5. 조사된 모든 대상은 작물재래종의 지속적인 보존재배를 위하여 각각의 특성을 살려 재래종 종자의 생산보급 또는 가공 판매사업과 연계하고 있는 것으로 나타났다. 따라서, 최근 거의 사라져 가는 작물재래종 자원의 농가보존(현지내보존)을 지속적으로 유지할 수 있는 일종의 사례가 될 수 있을 것으로 사료되었다. 6. 작물재래종 자원의 종자은행 보존과는 또 다른 의미를 갖는 농가현지 보존이 유지되기 위하여는 국가, 지방, 단체 및 개인에 이르기까지의 공동적이면서 지속적인 노력과 지원을 필요로 하였다. Farmers to cultivate landrace crops are almost essential to preserve native resources. It is time to be concerned about endangered landraces but very few farmers have their own know-how to keep and grow landraces. The case investigations were carried out during 2005 ~ 2008 to seek the way of on farm conservation of the landraces. The results can be summarized as follows. Shillim Nonghyup(Agricultural Cooperative Federation), Wonju which collected landraces of miscellaneous grain crops from whole country exhibits and preserves them at the field. The field is used as the base responsible for the supply of seeds and used also for education. The other hand, Shillim Nonghyup contracts with farmers to produce the landrace of miscellaneous grain crops which are sold in a small packages at the large retail stores and markets. Korean Women Farmer Association formed a consultative group of landrace seed dissemination (Seedream). Seedream has surveyed indigenous resources, produced landrace seeds, held the traditional agricultural workshops, and campaigned to keep the landrace seeds. Seungdang Farm Corporations of Agricultural Product Processing, Yeongwol specialized in domestic miscellaneous grain crop cultivation have purchased the seeds from contracted farmers and sold them through the large retail stores and markets. "Miscellaneous grain crops are medicines" Farm Corporations of Agricultural, Gangjin have purchased the seeds from contracted farmers and provide them for school lunches. Gyeongsangbuk-do Foundation Original Seed Production Center, Euiseong branch has collected landraces from whole country, preserved, produced, and supplied to farmers. Gyeongsangnam-do Foundation Original Seed Production Center runs the field for exhibition and seedbank. Agricultural Technology Center, Yeongju has conserved the landrace through the participation of the project to excavate the landrace soybean "Buseok" and miscellaneous grain crops. The Farm of Dawn, Jeongseon has conserved and cultivated the landraces soybean "Jwinoonikong" through production, processing and sales the processed items. The survey showed that all landraces were conserved through continuing production with the characteristics of their owns, dissemination, or process in connection with sales. Thus, this kind of cases can be a way to conserve the disappearing landraces recently from farmers. Efforts and supports by stakeholders including national and local governments, communities and individuals are required in order to maintain on farm conservation of the landraces.