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반하 및 차나무의 기내배양시 발생하는 세균의 동정 및 항생제 감수성 검정
김행훈,조규택,윤문섭,윤주원,조은기,Kim, Haeng-Hoon,Cho, Gyu-Taek,Yoon, Mun-Sup,Yoon, Ju-Won,Cho, Eun-Gi 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.2
Contamination of bacterial infection is one of serious problems in in vitro culture system of root crops. From the contaminated tubes over 140 of petiole cultures of Pinellia ternata, a medicinal plant, 4 genera 8species 48 strains of bacteria, including Aeromonas and Pseudomonas, were isolated and identified and another 8 strains were not fully identified. Most of them were motile Gram positive bacteria as in common in early stage of in vitro cultures. Six strains of bacteria, 5 of Gram negative, including Enterobacter, and 1 of Gram positive, were identified from the embryonic axes cultures of tea plant. From the susceptibility test to pre-screened 5 antibiotics, all of the bacteria except for 2 species of Pseudomonas were susceptible to cefotaxime 60∼100mg/L. While 60mg/L erythromycin only was effective to Pseudomonas. Combination of erythromycin 20mg/L and cefotaxime 60mg/L totally suppressed the growth of all bacterial strains tested. Susceptibility test of bacteria from tea embryonic axes cultures showed similar results. Combination of erythromycin 35mg/L and cefotaxime 60mg/L was effective to 15 bacterial strains and partially effective to 1 unidentified.
半夏의 器內 球莖形成에 미치는 炭素源, 窒素源, cytokinin 및 抗生劑의 效果
Hang Hun Kim(金杏勳),Young Am Chae(蔡永岩) 한국육종학회 1994 한국육종학회지 Vol.26 No.3
This experiment was carried out to know the factors affecting on corm formation in petiole cultures of Pinellia ternata (Thunb.) Breit. The results indicated that sucrose was the most effective carbon source for corm formation and fresh weight of corms was increased as the concentrations of sucrose up to 6%, optimum ratio of ammonium to nitrate was 1 : 3, zeatin was more effective than any other cytokinins and proper concentration was 1-2㎎/ℓ, polyamines such as putrescine and spermine had positive effect on corm enlargement, antibiotics such as carbenicillin, gedacylin and ampicillin stimulated corm fatness as well as antibacterial effect.