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( Minhyeok Lee ),( Ji Woong Son ),( Chang Ryul Park ),( Daeun Kang ),( Su Yel Lee ),( Se Jin Park ),( Wan Jin Hwang ),( Gwan Woo Ku ),( Seong Lan Yu ),( In Beom Jeong ),( Sun Jung Kwon ),( Jaeku Kang 대한결핵 및 호흡기학회 2021 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.129 No.-
Purpose Cancer stem cells (CSCs) identified in lung cancer exhibit resistance to chemotherapy, radiotherapy, and targeted therapy. Therefore, a technology to control of CSCs is needed to overcome such resistance to cancer therapy. Various evidences about the association between epithelial-mesenchymal transition related transcriptomic alteration and acquisition of CSC phenotype have been proposed recently. In our previous research, down-regulated miR-26a-5p is closely related to mesenchymal-like lung cancer cell lines. These findings suggest that miR-26a-5p might be involved in lung cancer stemness. Methods RNA polymerase III subunit G (POLR3G) was selected as a candidate target of miR-26a-5p related to cancer stemness. its quantitative relationship was investigated by polymerase chain reaction, western blot after transfection of miR-26a-5p. luciferase assay were done for investigating the direct regulation of miR-26a-5p on POLR3G expression. After transfection of miR-26a-5p, colony formation assay and sphere formation assay were performed to evaluate the effect on cancer stemness. By treating cancer cell by miR-26a-5p and paclitaxel, cell viability was checked by 3-(4,5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay and Muse cell analyzer. Expression level of each gene and its impact on survival were revealed by the cancer genome atlas pancancer database. Results miR-26a-5p regulated the expression of POLR3G directly. Overexpression of miR-26a-5p induced down regulation of POLR3G and a marked reduction of colony formation and sphere formation. Co-treatment of miR-26a-5p with paclitaxel decreased cell growth, suggesting that miR-26a-5p might play a role as a chemotherapy sensitizer. In the cancer genome atlas data, downregulated miR-26a-5p and up-regulated POLR3G were shown compared to adjacent normal tissue. High miR-26a-5p and low POLR3G expression were also related to higher survival rate of patients with lung adenocarcinoma. Conclusions Overexpression of miR-26a-5p can suppress lung cancer stemness and make cancer cell become sensitive to chemotherapy. This finding provides a novel insight into a potential lung cancer treatment by regulating stemness.
Park Se Jin,Kang Daeun,Lee Minhyeok,Lee Su Yel,Park Young Gyu,Oh TaeJeong,Jang Seunghyun,Hwang Wan Jin,Kwon Sun Jung,An Sungwhan,Son Ji Woong,Jeong In Beom 대한의학회 2024 Journal of Korean medical science Vol.39 No.2
Background: When suspicious lesions are observed on computer-tomography (CT), invasive tests are needed to confirm lung cancer. Compared with other procedures, bronchoscopy has fewer complications. However, the sensitivity of peripheral lesion through bronchoscopy including washing cytology is low. A new test with higher sensitivity through bronchoscopy is needed. In our previous study, DNA methylation of PCDHGA12 in bronchial washing cytology has a diagnostic value for lung cancer. In this study, combination of PCDHGA12 and CDO1 methylation obtained through bronchial washing cytology was evaluated as a diagnostic tool for lung cancer. Methods: A total of 187 patients who had suspicious lesions in CT were enrolled. PCDHGA12 methylation test, CDO1 methylation test, and cytological examination were performed using 3-plex LTE-qMSP test. Results: Sixty-two patients were diagnosed with benign diseases and 125 patients were diagnosed with lung cancer. The sensitivity of PCDHGA12 was 74.4% and the specificity of PCDHGA12 was 91.9% respectively. CDO1 methylation test had a sensitivity of 57.6% and a specificity of 96.8%. The combination of both PCDHGA12 methylation test and CDO1 methylation test showed a sensitivity of 77.6% and a specificity of 90.3%. The sensitivity of lung cancer diagnosis was increased by combining both PCDHGA12 and CDO1 methylation tests. Conclusion: Checking DNA methylation of both PCDHGA12 and CDO1 genes using bronchial washing fluid can reduce the invasive procedure to diagnose lung cancer.
Minhyeok Yoon,Jung Guen Cha,Jongmin Park,Sang Yub Lee,See Hyung Kim,Jihoon Hong,Byunggeon Park 대한영상의학회 2023 대한영상의학회지 Vol.84 No.5
Bronchial artery aneurysm (BAA) is a rare disease, and multiple aneurysms of a single bronchial artery are rarer. Regardless of the size of the lesion, it is at risk of rupture and can cause massive hemoptysis or severe pain. We report a rare case of bronchial artery embolization (BAE) of multiple aneurysms of a single bronchial artery. During medical examination, a 64-year-old female was diagnosed with multiple BAAs and endobronchial lesions in the right lower lung on CT 10 years prior to presentation to our hospital. Further evaluation of the lesions was recommended; however, the patient was lost to follow-up. The patient complained of dyspnea and visited our hospital, and the size of the BAA had increased on CT. BAE was done successfully using N-butyl-2-cyanoacrylate and detachable coils. Follow up CT after BAE showed significant decrease in extent of inflammatory lesion in the right lung.
Choe, Minhyeok,Park, Woojin,Kang, Jang-Won,Jeong, Sehee,Hong, Woong-Ki,Lee, Byoung Hun,Park, Seong-Ju,Lee, Takhee IOP Pub 2012 Nanotechnology Vol.23 No.48
<P>We investigated the threshold voltage instability induced by gate bias (V<SUB>G</SUB>) stress in ZnO nanowire (NW) field effect transistors (FETs). By increasing the V<SUB>G</SUB> sweep ranges and repeatedly measuring the electrical characteristics of the ZnO NW FETs, the V<SUB>G</SUB> stress was produced in the dielectric layer underneath the ZnO NW. Consequently, the electrical conductance of the ZnO NW FETs decreased, and the threshold voltage shifted towards the positive V<SUB>G</SUB> direction. This threshold voltage instability induced by the V<SUB>G</SUB> stress is associated with the trapping of charges in the interface trap sites located in the ZnO NW–dielectric interface. Our study will be helpful for understanding the stability of ZnO NW FETs during repetitive operations. </P>
박민혁(Minhyeok Park),박정수(Jungsoo Park),이운주(Woonjoo Lee) 대한기계학회 2021 대한기계학회 춘추학술대회 Vol.2021 No.11
Ball ramp in LSD system plays a role to support clutch engaging and disengaging at short duration. The applied torsional load is transferred abruptly and also receiving high axial force during clutch engaging. The precious gear geometry and high strength of ramp is needed. In this study, the ‘hybrid ball ramp’ is developed to satisfied those requirements. It consists of ANSI 8620 steel plates and a powdered gear with CD welding. The idea of this combination pursues the cost efficiency with high precision gear and sufficient strength of ramp during clutch engaging. The design is verified throughout the push-off and torsional test for welding strength and additionally the performance test and durability test at system level.