Effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase I after in vitro maturation

Yoon, Jeong,Juhn, Kyoung-Mi,Yoon, San-Hyun,Ko, Yong,Lim, Jin-Ho The Korean Society for Reproductive Medicine 2017 Clinical and Experimental Reproductive Medicine Vol.44 No.1

Objective: The aims of this study were to investigate whether fertilization could induce the resumption of meiosis in mouse oocytes arrested at metaphase I (MI) after in vitro maturation (IVM), and to investigate the effect of $Ca^{2+}$ chelator treatment at the time of fertilization on the transition from MI to metaphase II (MII). Methods: MII-stage and arrested MI-stage mouse oocytes after IVM were fertilized, and then embryonic development was monitored. Blastocysts from each group were transferred into 2.5 days post-coitum pseudo-pregnant ICR mice. MI oocytes after IVM were treated with a $Ca^{2+}$ chelator to investigate the effect of $Ca^{2+}$ oscillations on their maturation. Results: As insemination time increased, the number of oocytes in the MI group that reached the MII stage also increased. The blastocyst rates and total cell numbers in the MII group were significantly higher than in the MI group. No pregnancy occurred in the MI group, but 10 pregnancies were achieved (10 of 12) in the MII group. The proportion of MI oocytes that matured to MII oocytes after fertilization was significantly higher in the non-treated group than in the $Ca^{2+}$ chelator-treated group. Conclusion: The findings that a higher proportion of MI-arrested oocytes progressed to MII after fertilization and that the MI-to-MII transition was blocked by $Ca^{2+}$ chelator treatments before fertilization indicate that the maturation of MI oocytes to MII oocytes is associated with intracellular $Ca^{2+}$ oscillations driven by fertilization.

고방(古方)으로 치료한 두드러기 환자 39례의 임상보고(臨床報告)

탁명림,김미보,변석미,고우신,윤화정,Tark, Myoung-Rim,Kim, Mi-Bo,Byun, Seok-Mi,Ko, Woo-Shin,Yoon, Hwa-Jung 대한한방안이비인후피부과학회 2009 한방안이비인후피부과학회지 Vol.22 No.3

Objectives : This study was designed to investigate the chronical features of urticaria and the effects of urticaria treatment with Ko-Bang(古方). Methods : We treated 39 patients for urticaria with Ko-Bang(古方), who visited to care urticaria at the Dept. of Dermatology Oriental medical hospital Dong-eui university from Jan, 2008 to Sep, 2009. This study was assessed using the chart analysis and the follow-up survey by telephone. Results & Conclusions : 1. 39 outpatients were surveyed, who were 20 males and 19 females. And patients who were ages 21-30 were the largest group, 11(28.21%). Among the 39 patients, acute urticaria patients were 8(20.5%), chronic urticaria patients were 31(79.5%), and contraction of a disease period between 6 weeks with 3 months was 17.9%. 2. The causes of urticaria were measured unknown 37.5%, foods 37.5%, drugs 25% in acute and unknown 35.5%, a change of temperature 29%, foods 16% in chronic. And suspected provocation factors were measured unknown and foods each 12 cases. The most of accompanied symptoms was itching sign(34 cases), the second most was self-conscious fever(10 cases). 3. Out of treat prescription(Ko-Bang,古方), Injinho-Tang(茵蔯蒿湯) was used most in 5 cases, Daehwanghwangryunsasim-Tang(大黃黃連瀉心湯), Hwangryun-Tang(黃連湯), Daesiho-Tang(大柴胡湯) were used in each 4 cases, Galgeun-Tang(葛根湯), Chijadaehwangsi-Tang(梔子大黃?湯), Sosiho-Tang(小柴胡湯) were used in each 3 cases, Gaemagakban-Tang(桂麻各半湯), Chijasi-Tang(梔子?湯),Oryeongsan(五笭散) were used in each 2 cases. 4. When the processes of treatment were classified by one poison(一毒), there were the 19 cases (48.7%) which was caused by Bun(煩), the 12 cases(30.8%) by Water(水) and the 4 cases(10.3%) by Gyur(結).

미세각막절개 백내장 수술 후 낭내 삽입한 일체형 비구면 인공수정체의 임상 결과

김윤전,전미현,고동아,김재용,김명준,차흥원,Yoon Jeon Kim,Mi Hyun Cheon,Dong Ah Ko,Jae Yong Kim,Myoung Joon Kim,Hung Won Tchah 대한안과학회 2013 대한안과학회지 Vol.54 No.4

Purpose: To evaluate the stability and optical performance of the newly developed single-piece aspheric intraocular lens (IOL) by comparing the clinical outcome of the aspheric IOL with the new optic profile design (HOYA iSert, HOYA iMics) and the aspheric IOL (Akreos MI60), which has been proven effective and safe. Methods: iSert, iMics, and MI60 were inserted into 55 eyes, 60 eyes, and 50 eyes, respectively, after microincision phacoemulsification cataract surgery. Best corrected visual acuity (BCVA), refraction in spherical equivalent, anterior chamber depth (ACD), total higher order aberration (HOA), contrast sensitivity, and surgically induced astigmatism (SIA) were measured and each IOL was evaluated on the functional stability, anterior-posterior stability, centration in the capsular bag, and quality of vision. Results: No statistical differences in preoperative and postoperative BCVA among the 3 IOL groups were observed, however, MI60 showed significant myopic shift postoperatively. Anterior-posterior stability assessed with postoperative change in refractive error and ACD was slightly lower in the MI60 group. In terms of vision quality, while total aberration, total HOA, coma aberration, and contrast sensitivity for the 3 IOLs were not different significantly, spherical aberration of the MI60 group was higher than the other groups at 6 months postoperative. SIA was significantly increased in eyes implanted with iSert than in eyes with iMics or MI60 at 1 month postoperatively, however, the differences were no longer evident after 3 months postoperatively. Conclusions: The new aspheric IOLs, iSert and iMics, showed good stability and visual outcome equal to MI60 at the 6-month postoperative follow-up.

Effects of Instructional Treatments with Learning Conditions and Tasks on Learning of Simple and Complex Grammatical Rules

Mi-Sook Ko 한국영어어문교육학회 2008 영어어문교육 Vol.14 No.4

This study is a subsequent experiment of Ko’s (2006), which investigated the effects of learning conditions and task types respectively. Based on the findings of Ko’s (2006), the present study examined which combination of instructional treatments combining two learning conditions with two task types could be more effective than the other combinations on the learning of different grammatical rules. 130 Korean college freshmen with low proficiency were assigned to four experimental groups: EC (explicit condition and comprehension task), EP (explicit condition and production task), IC (implicit condition and comprehension task), and IP (implicit condition and production task). After two week instructions, post-test data through grammaticality judgment test and controlled written test were analyzed by the two-way Multiple Analysis of Covariance (MANCOVA). The findings of the study are as follows: (a) for the comprehension of a simple rule, there is no significant difference; (b) for the production of a simple and a complex rule, the EP can be the most effective; and (c) for the comprehension of a complex rule, the EP and the IP can be more effective than the EC and the IC. Overall, the effect of the combination with an explicit condition and a production task is more evident than the others. The results are expected to give empirical evidence and suggestions for more effective ways of promoting learning of formal linguistic features and leading L2 learners to develop their interlanguage.

말초성 안면마비 입원환자 250례에 대한 임상적 고찰

강나루,탁명림,변석미,고우신,윤화정,Kang, Na-Ru,Tark, Myoung-Rim,Byun, Soek-Mi,Ko, Woo-Shin,Yoon, Hwa-Jung 대한한방안이비인후피부과학회 2010 한방안이비인후피부과학회지 Vol.23 No.3

Objectives : This study was performed to analysis the effect of oriental medical care for inpatients with facial paralysis that had visited Dept. of Otolaryngology Oriental medical hospital Dong-eui university. Methods : From January 2008 to September 2010, a clinical study was done on 250 inpatients who were treated as facial nerve paralysis at the Dept. of Otolaryngology Oriental medical hospital Dong-eui university. This study was assessed using the chart analysis. Results : 1. The distribution of sex : female 54.8%, male 45.2%. The distribution of age was pregented that fifty to sixty was the most in 67 cases(26.8%). 2. The distribution of the period of admission : female 12.5 days, male 9.9 days. 3. The distribution of past history : hypertention(18.8%), diabetes-mellitus(10.85%), facial paralysis(9.25%), cerebrovascular disease(4.4%), liver disease(5.6%), hyperlipidemia(1.2%), otitis media(1.2%), herpes zoster(1.2%), cardiac disease(2.45%), thyroid disease(1.2%). 4. The distribution of the region of facial paralysis : Rt(55.36%), Lt(56.52%). 5. Check the mastoid pain : 66.8%(female 73.91%, male 58.04%). 6. Out of prescription(Ko-Bang, 古方), Galgeun-Tang(葛根湯) and Gaejigeogaegayoungchul-Tang was used most in each 34 cases, Daesiho-Tang(大柴胡湯) 30 cases, Galgeungabanha-Tang(葛根加半夏湯) 27 cases, Sihogaeji-Tang(柴胡桂枝湯) 14 cases, Hwanggigaejiomul-Tang 12 cases, Odu-Tang(烏頭湯) 10 cases, Chijadaehwangsi-Tang 10 cases, Gaejigagalgeun-Tang(桂枝加葛根湯) 7 cases, Banhasasim-Tang(半夏瀉心湯) 5 cases, Injinho-Tang(茵蔯蒿湯) 5 cases in order. 7. The distribution of herb group : Mahwang-Jae(麻黃劑) 31.72%, Gaeji-Jae(桂枝劑) 26.00%, Siho-Gae(柴胡劑) 20.70%, Chija-Gae(梔子劑) 7.49%, Buja-Jae(附子劑) 4.41%, Banhahwanggeum-Gae(半夏黃芩劑) 3.08%, Daehwang-Gae(大黃劑) 2.64%, Bockryeong-Gae(茯笭劑) 1.76%, Jisil-Gae(枳實劑) 1.32%, Insam-Gae(人蔘劑) 0.88% in order. 8. The distribution of House-Brackmann grade of admission : Gr Ⅳ 74.85%, Gr.III 13.6%, Gr.V 11.6% in order. 9. The distribution of House-Brackmann grade of discharge : Gr.III 56%, Gr.IV 38.4%, Gr.II 5.6% in order. 10. The average number of OPD follow up is 6.46. Conclusion : This results indicated that oriental medical treatment with Ko-bang(古方) can be an effective way to treat facial paralysis. The more patients we treat with Ko-bang(古方), the more clinical report is accumulated. Then it would be helpful to map out a systematic treatment on facial paralysis.

Identification of AFLP Marker Linked to a SCN Resistant Gene in Soybean

Ko,Mi-Suk,Kim,Myung-Sik,Han,Soung-Jin,Chung,Jong-Il,Kang,Jin-Ho 한국자원식물학회 2002 Plant Resources Vol.5 No.3

The soybean cyst nematode (Heterodera glycines Inchinoe; SCN) is a devastating pest of soybean and is responsible for significant losses in yield. The use of resistant cultivars is the effective method to reduce or eliminate SCN damage. The objective of this research is to identify AFLP markers linked to the SCN resistant genes. Bulked genomic DNA was made from resistant and susceptible genotypes to SCN and a total of 19 primer combinations were used. About 31 fragments were detected per primer combination. The banding patterns were readily distinguished in resistant and susceptible bulked genotypes. Polymorphic fragments were detected between resistant and susceptible bulked genotypes in the primer combination of CGT/GGC, CAG/GTG and CTC/GAG. In primer combinations of CGT/GGC and CAG/GTG, bulked resistant genotype produced a polymorphic bands. However, in primer of CTC/GAG, bulked susceptible genotype produced a polymorphic fragments. Three AFLP markers identified as a polymorphic fragments between bulked genomic DNA were mapped in 85 F2 population. Among them, only two markers, CGT/GGC and CTC/GAG, was linked and was mapped. Broad application of AFLP marker would be possible for improving resistant cultivars to SCN.

Recent strategies of increasing metal tolerance and phytoremediation potential using genetic transformation of plants

Aleksandra Koźmińska,Alina Wiszniewska,Ewa Hanus‑Fajerska,Ewa Muszyńska 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.1

Avoidance and reduction of soil contamination with heavy metals is one of the most serious global challenges. Nowadays, science offers us new opportunities of utilizing plants to extract toxic elements from the soil by means of phytoremediation. Plant abilities to uptake, translocate, and transform heavy metals, as well as to limit their toxicity, may be significantly enhanced via genetic engineering. This paper provides a comprehensive review of recent strategies aimed at the improvement of plant phytoremediation potential using plant transformation and employing current achievements in nuclear and cytoplasmic genome transformation. Strategies for obtaining plants suitable for effective soil clean-up and tolerant to excessive concentrations of heavy metals are critically assessed. Promising directions in genetic manipulations, such as gene silencing and cis- and intragenesis, are also discussed. Moreover, the ways of overcoming disadvantages of phytoremediation using genetic transformation approachare proposed. The knowledge gathered here could be useful for designing new research aimed at biotechnological improvement of phytoremediation efficiency.

일 도농복합지역 저소득층 노인의 건강문제 분석 : 보건소 방문간호사업 대상자 중심으로

고일선,,이태화,이경자,이정렬,임미혜,천의영,주윤미,이계철 노인간호학회 2005 노인간호학회지 Vol.7 No.2

Purpose: The purpose of this study was to analyze the health problems of low-income aged with chronic illness living in urban-rural composite area. Method: The sample consisted of 440 aged who were receiving home care services from a public health center. MDS-HC was applied to analyze the health problems of the aged. Data were collected through a face- to-face interview by six trained interviewers from June 28 to July 15, 2004. Result: Subjects had average number of 8 health problems in both urban and rural area. Lack of preventive health care measure, pain, and visual function were the most frequent health problem. The rural aged had more pain, bowel management problems, compared to the urban aged having more urinary incontinence and indwelling catheter. There were many health problems related to falls and pressure ulcers with middle-old aged in urban, and old-old aged in rural area. Conclusion: The results of this study showed strategies for care intervention of low-income elderly to put in practice. Therefore, tailored-service for each subject should be provided.

인산염 처리에 따른 콩과 및 벼과 식물의 유기산 분비 및 인흡수량 차이

고미라,송성준,강영길 제주대학교 방사능이용연구소 2005 방사선응용과학연구소논문집 Vol.19 No.-

지시교육과 도덕교육

고미숙 고려대학교 교육문제연구소 2002 敎育問題硏究 Vol.0 No.17

본 논문은 지·행의 괴리를 해결하기 위한 방안으로 지식교육과 도덕교육의 관계를 탐구하고자 한다. 지식교육과 도덕교육의 관계는 '지식'과 '지식교육'의 의미를 무엇으로 보는가에 따라서 달라질 수 있다. 본 논문은 지식의 개념을 전통적 인식론과 페미니스트 인식론으로 구분하여 살펴본다. 이에 명제적 지식에만 초점을 두는 전통적 인식론과 지식교육을 비판하고, 실천적 지식과 방법적 지식의 중요성을 지적한다. 또한 지식교육과 도덕교육의 관계를 크게 두 가지 입장에서 논의한다. 첫째, 지식교육은 도덕교육의 충분조건이 될 수 있는가? 전통적 인식론에 근거한 지식교육은 도덕교육에 도움이 될 수 있기는 하지만 지식교육만으로 도덕교육이 이루어질 수 있는 것은 아니라고 본다. 그 이유는 지식교육에 대한 강조가 인간의 마음의 다른 측면들인 정서, 감정, 태도 등의 교육을 소홀히 하도록 한다는 것과 지적인 교과에서 가르치는 덕도 주로 인지적인 정서와 관련된 지적인 덕이며, 이러한 교육만으로는 도덕교육에 충분하지 않다고 보기 때문이다. 둘째, 도덕교육에서 도덕적 지식과 도덕적 행위사이의 관계를 어떻게 파악해야 할 것인가? 본 논문은 도덕적 앎이 도덕적 행위에 반드시 선행하는 것은 아니라고 보며, 앎은 실천으로부터 나온다는 관점을 옹호한다. 이것으로부터 본 논문은 몸 지식의 중요성을 지적하면서 체현의 도덕교육을 주장한다. This article is to explore the relationship between the education of knowledge and moral education in order to bridge the gap between knowing and behaviour. The relationship between the education of knowledge and moral education depends on the concept of 'knowledge' and 'the education of knowledge'. I divide the concept of knowledge into traditional epistemology and feminist epistemology. In doing so, I criticize traditional epistemology and the education of knowledge focusing on 'propositional knowledge' and emphasize the importance of 'practical knowledge' and 'knowledge how' according to feminist epistemology. Also I examine the relationship between the education of knowledge and moral education from the two points of view. Firstly, is the education of knowledge sufficient to moral education? I think that moral education does not consist of the education of knowledge only, although the education of knowledge based on traditional epistemology is useful in moral education. The reason is that the emphasis on the education of knowledge leads to ignore feelings, emotions, attitudes and so on, in addition, virtues developing in intellectual subjects are intellectual virtues related mainly cognitive emotions. Therefore, the education of knowledge is not sufficient to moral education. Secondly, how do we understand the relationship between moral knowledge and moral behaviour in moral education? Moral knowing does not necessarily precede moral behaviour. I support that knowing arises out of practice. As a result of this, I propose moral education of embodiment emphasizing the importance of body knowledge.