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<i>Ex Vivo</i> Characteristics of Human Amniotic Membrane-Derived Stem Cells
Kim, Jiyoung,Kang, Hyun Mi,Kim, Haekwon,Kim, Mee Ran,Kwon, Hyuck Chan,Gye, Myung Chan,Kang, Sung Goo,Yang, H. Seung,You, Juice Mary Ann Liebert 2007 Cloning & stem cells Vol.9 No.4
<P>Cells were isolated from four human amniotic membranes, and their biological characteristics analyzed during ex vivo expansion. Morphologically homogenous populations of fibroblast-like cells were obtained from the second or third passage. Under the appropriate culture conditions, these human amniotic membrane-derived mesenchymal cells (HAM) were shown to differentiate into adipocytes, osteocytes, chondrocytes and neuronal cells, as visualized by Oil Red O, von Kossa, alcian blue, anti-Neu N, and anti-Gal C antibody staining, respectively. Immunophenotype analysis of HAM cells revealed the presence of antigens for SSEA-3, SSEA-4, collagen type-I, -II, -III, -IV, -XII, fibronectin, alpha-SMA, vimentin, desmin, cytokeratin18 (CK18), HCAM-1, fibroblast surface protein, and human leukocyte antigen (HLA) ABC. ICAM-1 protein was weakly detectable, and proteins of TRA-1-60, VCAM-1, von Willebrand factor, PECAM-1, and HLA DR were not detected. HAM cells reached senescence after 14.5+/-0.9 passages, over a period of 146.8+/-8.9 days, and underwent an average of 36.9 4.7 population doublings. RT-PCR analysis showed that all four HAM cell lines consistently expressed genes of Oct-4, Rex-1, SCF, NCAM, nestin, BMP-4, GATA-4, HNF-4alpha, vimentin, and CK18, regardless of the passage number. The genes of Brachyury, FGF-5, Pax-6, and BMP2 were never expressed. Strikingly, alpha-fetoprotein (alphaFP), HLA ABC, and HLA DR genes were expressed in an earlier passage but not expressed in later passages. Telomerase activity of two HAM lines was discernable upon the third passage. These observations strongly suggest that HAM might be immune-privileged and, thus, advantageous as therapeutic cells.</P>
Kim Hokun,Rha Sung Eun,Shin Yu Ri,Kim Eu Hyun,Park Soo Youn,Lee Su-Lim,Lee Ahwon,Kim Mee-Ran 대한영상의학회 2024 Korean Journal of Radiology Vol.25 No.1
Objective: To evaluate the added value of diffusion-weighted imaging (DWI)-based quantitative parameters to distinguish uterine sarcomas from atypical leiomyomas on preoperative magnetic resonance imaging (MRI). Materials and Methods: A total of 138 patients (age, 43.7 ± 10.3 years) with uterine sarcoma (n = 44) and atypical leiomyoma (n = 94) were retrospectively collected from four institutions. The cohort was randomly divided into training (84/138, 60.0%) and validation (54/138, 40.0%) sets. Two independent readers evaluated six qualitative MRI features and two DWI-based quantitative parameters for each index tumor. Multivariable logistic regression was used to identify the relevant qualitative MRI features. Diagnostic classifiers based on qualitative MRI features alone and in combination with DWI-based quantitative parameters were developed using a logistic regression algorithm. The diagnostic performance of the classifiers was evaluated using a cross-table analysis and calculation of the area under the receiver operating characteristic curve (AUC). Results: Mean apparent diffusion coefficient value of uterine sarcoma was lower than that of atypical leiomyoma (mean ± standard deviation, 0.94 ± 0.30 10-3 mm2/s vs. 1.23 ± 0.25 10-3 mm2/s; P < 0.001), and the relative contrast ratio was higher in the uterine sarcoma (8.16 ± 2.94 vs. 4.19 ± 2.66; P < 0.001). Selected qualitative MRI features included ill-defined margin (adjusted odds ratio [aOR], 17.9; 95% confidence interval [CI], 1.41–503, P = 0.040), intratumoral hemorrhage (aOR, 27.3; 95% CI, 3.74–596, P = 0.006), and absence of T2 dark area (aOR, 83.5; 95% CI, 12.4–1916, P < 0.001). The classifier that combined qualitative MRI features and DWI-based quantitative parameters showed significantly better performance than without DWI-based parameters in the validation set (AUC, 0.92 vs. 0.78; P < 0.001). Conclusion: The addition of DWI-based quantitative parameters to qualitative MRI features improved the diagnostic performance of the logistic regression classifier in differentiating uterine sarcomas from atypical leiomyomas on preoperative MRI.
Loss of heterozygosity of chromosome 3p in patients with carcinoma of the cervix
Kim, Jin Woo,Hong, Sung Ran,Park, So Yeon,Ryu, Hyun Mee,Park, Chong Taik,Lee, Ki Heon,Chung, Hwan Wook,Lim, Kyung Taek,Seong, Seok Ju,Park, In Sou,Shim, Jae Uk,Kim, Tae Jin 대한부인종양 콜포스코피학회 2002 Journal of Gynecologic Oncology Vol.13 No.1
Effect of Salinity, Temperature, and Glucose on the Production of Vibrio vulnificus Hemolysin
Kim, Hyun-Soo,Shin, Sung-Heui,Park, Hae-Ryoung,Lee, Shee-Eun,Kim, Choon-Mee,Kim, Soo-Young,Kim, Young-Ran,Lee, Hyun-Chul,Chung, Sun-Sik,Rhee, Joon-Haeng The Korean Society for Microbiology 2002 Journal of Bacteriology and Virology Vol.32 No.4
Among the exotoxins produced by V. vulnificus, hemolysin (HS) has been reported to be the most potent one. To investigate the factors up- or down-regulating HS production in the context of pathogenesis, we observed the effects of salinity or/and temperature shifting, glucose, and acidic pH on the production of HS by V. vulnificus C7184 strain in vitro. Significantly more HS was produced when V. vulnificus was cultured in 0.9% salinity and $37^{\circ}C$ than in 2.5% and $25^{\circ}C$. When the culture condition reflecting natural habitat of V. vulnificus (2.5% salinity and $25^{\circ}C$) was changed into that reflecting human body (0.9% salinity and $37^{\circ}C$), 2.5 fold or more HS was produced than in the V. vulnificus being cultured continuously in 0.9% NaCl at $37^{\circ}C$. This result suggests that V. vulnificus somehow recognizes the shifting in salinity and temperature and stimulate HS production. Glucose addition in the culture medium resulted in a dose-dependent decrease in the HS production. Glucose itself and acidic pH resulting from its metabolism both appeared to inhibit the HS production. Glucose in itself had more dominant role in suppressing the HS production than the lowered pH accompanying the metabolism of glucose. This result suggests that HS production is down-regulated in the presence of glucose and under environmental acidic pH.
Kim, Young-Ran,Kim, Choon-Mee,Rhee, Joon-Haeng The Korean Society for Microbiology 2003 Journal of Bacteriology and Virology Vol.33 No.1
Live Vibrio vulnificus is highly cytotoxic to host cells in vivo and in vitro. The two most representative cytotoxins, cytolytic hemolysin and elastolytic protease, have been regarded to play major roles in the cytotoxicity of V. vulnificus. To further determine roles of the two cytotoxins in V. vulnificus pathogenesis, we constructed a double mutant of vvhA and vvpE genes, encoding a hemolysin and a protease, respectively. However, the cytotoxicity and the $LD_{50}$ of a vvhA/vvpE double mutant showed no significant difference from those of the isogenic wild type strain. From these results, we came to speculate that yet unidentified, key cytotoxic factors should playa major role in the cytotoxic activity of V. vulnificus. The HeLa cells encountered with V. vulnificus became rounded, following detachment from the bottom of culture plate, and were killed eventually. However, the bacterial culture supernatant did not show any effect on the morphology and viability of HeLa cells. Also, no cytotoxicity could be noted when V. vulnificus was not allowed to contact with HeLa cells in the $Transwell^{(R)}$ system. Chloramphenicol, at lower concentration than minimum inhibitory concentration (MIC), decreased the cytotoxicity of a vvhA/vvpE double mutant to HeLa cells in a dose dependent manner. These results suggest that close encounter of V. vulnificus with host cells is a prerequisite to the cytotoxicity and that a yet unidentified virulence factor (s) should play an important role in the contact-dependent cytotoxicity.
Essential role of an adenylate cyclase in regulating <i>Vibrio vulnificus</i> virulence
Kim, Young Ran,Kim, Soo Young,Kim, Choon Mee,Lee, Shee Eun,Rhee, Joon Haeng Elsevier 2005 FEMS microbiology letters Vol.243 No.2
<P><B>Abstract</B></P><P><I>Vibrio vulnificus</I>, a halophilic estuarine bacterium, causes a fatal septicemia and necrotizing wound infection. To investigate the role of cAMP in <I>V. vulnificus</I> virulence regulation, an in-frame deletion mutant of the <I>cya</I> gene encoding adenylate cyclase was constructed. The <I>cya</I> null mutation resulted in a pleiotropic change of virulence phenotypes. The production of hemolysin and protease, the motility, and the cytotoxicity were decreased by the <I>cya</I> mutation. The defects in the <I>cya</I> mutant were functionally complemented <I>in trans</I> by a plasmid carrying the wild type <I>cya</I> allele. The <I>V. vulnificus cya</I> mutant exhibited a 100-fold increase in LD<SUB>50</SUB> to mice. The result indicates that cAMP plays an essential role in the global regulation of <I>V. vulnificus</I> virulence.</P>
A predictive factor of total operation time for robotic-assisted laparoscopic myomectomy
( Chaewon Kim ),( Seong Eun Bak ),( Kyung-min Park ),( Suk-il Kim ),( Mee-ran Kim ) 대한산부인과학회 2019 대한산부인과학회 학술대회 Vol.105 No.-
Objective: The aim of our study is to find out the predictors of TOT in RALM. It will help selecting an adequate patient pool, enhancing preoperative counseling, optimizing operation schedule, and systemizing the intraoperative preparation process, thus improving the perioperative outcomes and patients’ satisfaction. Methods: We conducted a retrospective cohort study of women undergoing RALMs between April 2009 and May 2019 by a single high-volume gynecologic surgeon at the Seoul St. Mary’s hospital. Cases that were performed concomitantly with any other surgical procedure were excluded. Demographic data and intraoperative records were obtained. The association between the number, size, location, FIGO type of myoma and TOT was analyzed. Results: A total of 616 cases met our criteria, with a mean TOT of 234 ± 88 minutes. The mean age of patients was 36.0 ± 5.4. The mean number of myomas removed was 4.2 ± 4.0 and mean largest myoma diameter was 7.7cm ± 2.6cm. Mean total weight of myoma removed was 201.4 ± 151.7g. Only one patient underwent conversion to laparotomy. Preoperative factors intimately associated with TOT were identified with the multiple regression analysis. TOT prolonged as the number of myoma increases (β = 11.69, P < 0.0001) or total myoma weight increases (β = 0.18, P < 0.0001). If the endometrial cavity was exposed during the operation, longer surgery time was needed (β = 23.62, P = 0.002). Age, parity, body mass index, personal history of hypertension and diabetes mellitus, previous abdominal surgery, surgical indication, location of dominant myoma and classification of dominant myoma were not associated with TOT. Conclusion: In this study, we found the total number of myomas, myoma weight, and exposure of endometrial cavity to be significantly correlated to TOT of RALM.
Successfully removed uterine angioleiomyoma by robot-assisted laparoscopic myomectomy
( Hyunkyung Kim ),( Jin-ju Lee ),( Yonghee Choi ),( Minkyoung Lee ),( Hye-jin Hwang ),( Youn-jee Chung ),( Hyun-hee Cho ),( Mee-ran Kim ) 대한산부인과학회 2018 Obstetrics & Gynecology Science Vol.61 No.3
Angioleiomyoma is a rare type of leiomyoma variant and there are a few cases reported to date. Herein, we present a case of angioleiomyoma in a 36-year-old woman with lower abdominal pain, initially diagnosed by degenerated uterine leiomyoma. The transvaginal ultrasonogram showed an ovoid-shaped heterogeneously hyperechoic lesion in left cornual site of uterus and pelvic magnetic resonance image showed an about 5.1 cm sized heterogenous T2 intermediate high mass with poor enhancement. The patient underwent a robot-assisted laparoscopic myomectomy, and final histopathologic diagnosis revealed uterine angioleiomyoma. This case is the first case of angioleiomyoma resected by robotic surgery. The patient is on follow up for over 1 year and shows no evidence of recurrence until now.