http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
송준영,김미숙,전미령 조선대학교 스포츠과학연구소 1998 스포츠科學硏究 Vol.9 No.-
To recover humanity, forfeited by development of material civilization inconsequence of high growth of economy with harmony of nature and to create a spiritually affluent society and culture, importance of dance is increasing. The present study examined development process of Korean Folk Dance and presents plans for popularization of Korean Folk Dance to develope and revitalize Korean Folk Dance. To develope and popularize Korean folk Dance, we need following plans. 1. Extending and renovating the educational system. 2. Categorizing traditional dances and using repertory system. 3. Using mass-media and activating criticism. 4. Activating dance performance in little theaters and diversifying performance places.
Mi-Ryung Park,Jae Gyu Yoo,Chang-Gi Hur,Bo-Woong Sim,Myunghoo Kim,Jakyeom Seo,Byeong-Woo Kim,Byung-Wook Cho,Teak-Soon Shin,Seong-Keun Cho 한국동물생명공학회(구 한국동물번식학회) 2020 Journal of Animal Reproduction and Biotechnology Vol.35 No.3
This study investigated the effect of variation in the number of somaticcell- cloned embryos and their developmental stage at transfer on pregnancy, as well as the influence of the estrus status of recipient pigs on in vivo development of cloned porcine embryos after embryo transfer. For somatic cell nuclear transfer (SCNT), fibroblast cells were obtained from a male porcine fetus. Recipient oocytes were collected from prepubertal gilts at a local abattoir and then cultured. After SCNT, reconstructed embryos of different numbers and developmental stages were transferred into recipient pigs. The developmental stage of the cloned embryos and the number of transferred embryos per surrogate showed no significant differences in terms of the resulting cloning efficiency. However, the pregnancy rate improved gradually as the number of transferred cloned embryos was increased from 100- 150 or 151-200 to 201-300 per recipient. In pre-, peri-, and post-ovulation stages, pregnancy rates of 28.6%, 41.8%, and 67.6% and 16, 52, and 74 offspring were recorded, respectively. The number of cloned embryos and estrus status of the recipient pig at the time of transfer of the cloned embryo affect the efficiency of pig production; therefore, these variables should be particularly considered in order to increase the efficiency of somatic cell pig cloning.
Analysis of Glycated Serum Proteins in Type 2 Diabetes Patients with Nephropathy
Kim, Mi-Ryung,Yu, Shin-Ae,Kim, Mi-Yeon,Choi, Kyung Mook,Kim, Chan-Wha 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.1
The aim of this study was to screen for proteins that are susceptible to glycation under hyperglycemic conditions in patients with type 2 diabetic nephropathy. Serum proteins were analyzed by a proteomic approach using two-dimensional electrophoresis (2-DE) and ESI-Q-TOF MS/MS. Gels were stained with Pro-Q Emerald 488 to analyze the serum glycoproteome, followed by silver nitrate to examine the total serum proteome. Patient sera were divided into four groups according to their microalbuminuria index: type 2 diabetics with normoalbuminuria, microalbuminuria, and overt nephropathy, and healthy subjects. When the HbA1c levels of the diabetic groups were examined, groups with higher HbA1c exhibited higher fructosamine levels, suggesting that the loss of glycemic control affected the glycation of serum proteins. The proteins that became glycated under poor glycemic control were PEDF, apolipoprotein J precursor, hemopexin, immunoglobulin mu heavy chain, and immunoglobulin kappa chain. As albuminuria increased, a marker of kidney damage, the levels of glycated prekallikrein and complement factor C4B3 also increased. The glycated proteins identified in this study may provide the foundation for the development of novel markers of diabetes, hyperglycemia, and diabetic complications.
Proteomic Profiling of Undifferentiated hBM Mesenchymal Stem Cells under High Glucose Conditions
( Mi Ryung Kim ),( Ae Jin Choi ),( Chan Wha Kim ) 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.14
Bone marrow mesenchymal stem cells (BM-MSCs) have been suggested to participate in the physiologic healing process of tissue injury by being recruited from the bone marrow and home into the injured site through circulation. To investigate the effect of diabetic hyperglycemia on circulating BM MSCs, which would affect their differentiation, two-dimensional electrophoresis (2-DE) was performed to determine the pattern of protein expression in BM MSCs. After exposure to high (25 mM) glucose concentration for 3 days, six proteins were found to be down regulated by less than 50% (ERp29, Triosephosphate, ATP synthase, phosphoprotein enriched in asprocytes 15, putative glycogen phosphorylase and myosin light chain 3) relative to the control. In addition, 2 proteins (LPLUNC1, isoAsp carboxyl methyltransferase) were neo expressed and one (beta-galactoside binding lectin) protein was shown to be up regulated by more than 300% as a result of hyperglycemia. These results may provide a framework for understanding the molecular mechanism and relationship between hyperglycemia and stem cells.
The Polymorphism and Gene Frequencies of Haptoglobin Phenotypes in Korean
Kim,Hye Ryung,Kim,sook Han,Park,Kyung Sook,Kang Hyun Mi 성신여자대학교 기초과학연구소 1985 基礎科學硏究誌 Vol.2 No.-
Haptoglobin의 다형현상을 5% polyacrylamide gel electrophoresis로 조사하였다. 600명의 무관한 개체사이에서 Hp1-1, Hp2-1, Hp2-2의 세가지 표현형이 관찰되었으며 변형은 발견되지 않았다. Hp*1과 Hp*2에 대한 대립유전자 빈도는 각각 0.2884와 0.7116으로 측정되었다. 한국인 집단에서의 haptoglobin의 다형적 표현형과 유전자빈도를 다른 집단과 비교하였다. Polymorphism of the haptoglobin were investigated by 5% polyacrylamide gel electrophoresis. Among 600 unrelated individuals, three phenotypes Hp 1-1, Hp 2-1 and Hp 2-2 have been observed, but no variants were detected. The estimated allele frequencies for Hp*1 and Hp*2 were 0.2884 and 0.7116, respectively. The gene frequencies and polymorphic phenotypes of haptoglobin in Korean were compared with those in other populations.