Imbalanced bone turnover markers and low bone mineral density in patients with osteonecrosis of the femoral head

Tian, Lulu,Baek, Seung-Hoon,Jang, JinAn,Kim, Shin-Yoon Springer-Verlag 2018 International orthopaedics Vol.42 No.7

Preparation and deposition mechanism of pyrolytic carbon by CVI using 3D Ni/wood-carbon catalyst

Lulu Han,Xiaohong Shi,Xu Han,Li Yang,Kun Li,Tian Xinfa,Mu Jierui,Wang Guoqing 한국탄소학회 2022 Carbon Letters Vol.32 No.1

To improve the pyrolytic carbon (PyC) deposition rate of Carbon/Carbon (C/C) composites prepared by the traditional chemical vapor infiltration (CVI) method, the 3D Ni/wood-carbon (3D Ni/C) catalyst was introduced into the CVI process. The effects of catalyst on the density of C/C composites were studied, and the deposition rate and morphologies of PyC were investigated after catalytic CVI. The morphologies of catalyst and PyC were characterized by scanning electron microscope and polarized light microscopy. The catalytic deposition mechanism of PyC was studied by density functional theory. The experimental results show that the initial carbon deposition efficiency of the catalytic pyrolysis process was 3–4 times that of the noncatalytic process. The catalyst reduced the energy barrier in the first step of deposition reaction from 382.55 to 171.67 kJ/mol according to simulation results. The pyrolysis reaction energy with Ni catalyst is reduced by 54% than that without the catalyst.

Severe choline deficiency induces alternative splicing aberrance in optimized duck primary hepatocyte cultures

Zhao Lulu,Cai Hongying,Wu Yongbao,Tian Changfu,Wen Zhiguo,Yang Peilong 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.11

Objective: Choline deficiency, one main trigger for nonalcoholic fatty liver disease (NAFLD), is closely related to lipid metabolism disorder. Previous study in a choline-deficient model has largely focused on gene expression rather than gene structure, especially sparse are studies regarding to alternative splicing (AS). In modern life science research, primary hepatocytes culture technology facilitates such studies, which can accurately imitate liver activity in vitro and show unique superiority. Whereas limitations to traditional hepatocytes culture technology exist in terms of efficiency and operability. This study pursued an optimization culture method for duck primary hepatocytes to explore AS in choline-deficient model. Methods: We performed an optimization culture method for duck primary hepatocytes with multi-step digestion procedure from Pekin duck embryos. Subsequently a NAFLD model was constructed with choline-free medium. RNA-seq and further analysis by rMATS were performed to identify AS events alterations in choline-deficency duck primary hepatocytes. Results: The results showed E13 (embryonic day 13) to E15 is suitable to obtain hepatocytes, and the viability reached over 95% by trypan blue exclusion assay. Primary hepatocyte retained their biological function as well identified by Periodic Acid-Schiff staining method and Glucose-6-phosphate dehydrogenase activity assay, respectively. Meanwhile, genes of alb and afp and specific protein of albumin were detected to verify cultured hepatocytes. Immunofluorescence was used to evaluate purity of hepatocytes, presenting up to 90%. On this base, choline-deficient model was constructed and displayed significantly increase of intracellular triglyceride and cholesterol as reported previously. Intriguingly, our data suggested that AS events in choline-deficient model were implicated in pivotal biological processes as an aberrant transcriptional regulator, of which 16 genes were involved in lipid metabolism and highly enriched in glycerophospholipid metabolism. Conclusion: An effective and rapid protocol for obtaining duck primary hepatocytes was established, by which our findings manifested choline deficiency could induce the accumulation of lipid and result in aberrant AS events in hepatocytes, providing a novel insight into various AS in the metabolism role of choline. Objective: Choline deficiency, one main trigger for nonalcoholic fatty liver disease (NAFLD), is closely related to lipid metabolism disorder. Previous study in a choline-deficient model has largely focused on gene expression rather than gene structure, especially sparse are studies regarding to alternative splicing (AS). In modern life science research, primary hepatocytes culture technology facilitates such studies, which can accurately imitate liver activity <i>in vitro</i> and show unique superiority. Whereas limitations to traditional hepatocytes culture technology exist in terms of efficiency and operability. This study pursued an optimization culture method for duck primary hepatocytes to explore AS in choline-deficient model.Methods: We performed an optimization culture method for duck primary hepatocytes with multi-step digestion procedure from Pekin duck embryos. Subsequently a NAFLD model was constructed with choline-free medium. RNA-seq and further analysis by rMATS were performed to identify AS events alterations in choline-deficency duck primary hepatocytes.Results: The results showed E13 (embryonic day 13) to E15 is suitable to obtain hepatocytes, and the viability reached over 95% by trypan blue exclusion assay. Primary hepatocyte retained their biological function as well identified by Periodic Acid-Schiff staining method and Glucose-6-phosphate dehydrogenase activity assay, respectively. Meanwhile, genes of <i>alb</i> and <i>afp</i> and specific protein of albumin were detected to verify cultured hepatocytes. Immunofluorescence was used to evaluate purity of hepatocytes, presenting up to 90%. On this base, choline-deficient model was constructed and displayed significantly increase of intracellular triglyceride and cholesterol as reported previously. Intriguingly, our data suggested that AS events in choline-deficient model were implicated in pivotal biological processes as an aberrant transcriptional regulator, of which 16 genes were involved in lipid metabolism and highly enriched in glycerophospholipid metabolism.Conclusion: An effective and rapid protocol for obtaining duck primary hepatocytes was established, by which our findings manifested choline deficiency could induce the accumulation of lipid and result in aberrant AS events in hepatocytes, providing a novel insight into various AS in the metabolism role of choline.

Resistance Reduction Scheme for Tower Grounding with Conductive Cement

Lun Li,Tian Xu,Pei Feng,Liu Xin,Jia Lulu,Deng Chenxing,Wang Xing,Lan Fen,Cheng Hongbo 대한전기학회 2021 Journal of Electrical Engineering & Technology Vol.16 No.3

The construction conditions of transmission lines are becoming more and more diffi cult. It is necessary to fi nd new ways to reduce the grounding resistance of poles and towers at high soil resistivity and small construction area. Conductive cement samples with graphite, stainless steel fi bers, and their mixtures as conductive phases materials were prepared, the resistivity, fl exural strength and compressive strength were tested, compared and analyzed. The graphite and stainless steel fi ber conductive cement with better comprehensive performance was selected out for tower grounding. The application schemes of conductive cement for tower grounding were designed and compared, the partial wrapping scheme was selected and its application methods under diff erent soil resistivity was analyzed. The analysis results can provide theoretical basis and guidance for the application of conductive cement in transmission tower grounding, the feasibility of scheme is proved by the fi eld application.

Environmentally Friendly Waterproof and Breathable Nanofiber Membranes with Thermal Regulation Performance by One-Step Electrospinning

Pan Zhang,Gaihuan Ren,Lulu Tian,Bo Li,Zhanzhao Li,Hongqin Yu,Rongwu Wang,Jianxin He 한국섬유공학회 2022 Fibers and polymers Vol.23 No.8

The waterproof and moisture permeable fabric with thermal regulation not only has the protective function forhuman body, but also meets the comfort of human clothing, which is a challenging and innovative research. Herein, we reportan environmentally friendly, simple, and high-efficiency strategy to fabricate waterproof and breathable nanofibermembranes with outstanding thermal regulation ability by introducing stearic acid (SA) into the hydrophobic siliceouspolyurethane (SIPU) solution by a facile one-step electrospinning. Owing to the addition of SA, the waterproofness of SIPU/SA nanofiber membranes is greatly improved, meanwhile, the addition of SA acted as a phase change material to imparttemperature regulation performance in appropriate temperature range to the SIPU/SA nanofiber membranes. By adjusting theaddition amount of the SA, eco-friendly and multifunctional SIPU/SA nanofiber membranes are systemically optimized withgood waterproofness (79 kPa), moisture breathability (8.0 kg m-2 d-1), and outstanding temperature regulation performance(latent heat up to 40 J/g), satisfied tensile strength (6.2 MPa) and excellent breaking elongation of 182 %. The obtained SIPU/SA nanofiber membranes can be suitable for the design of multifunctional wearable textiles for various applications,especially in outdoor protective clothing, medical clothing, intelligent clothing and military products.

Surgical correction for Tessier number 7 craniofacial cleft using a medially overcorrected design

Ryu, Jeong Yeop,Eo, Pil Seon,Tian, Lulu,Lee, Joon Seok,Lee, Jeong Woo,Choi, Kang Young,Yang, Jung Dug,Chung, Ho Yun,Cho, Byung Chae Korean Society of Plastic and Reconstructive Surge 2019 Archives of Plastic Surgery Vol.46 No.1

Background Various surgical techniques have been used to correct Tessier number 7 craniofacial cleft, which involves macrostomia, ear deformity, and hemifacial microsomia. To achieve symmetrical and satisfactory results in patients with macrostomia, the authors performed a 1-mm medial overcorrection on the cleft side and evaluated the results of this procedure. Methods A retrospective medical record review of patients diagnosed with Tessier number 7 craniofacial cleft from March 1999 to February 2017 was performed. Using clinical photographs, outpatient clinic records, and operative records, information was recorded regarding concurrent congenital anomalies, postoperative complications, and follow-up. Using Photoshop CS2, the length of both sides of the lip was compared. The ratio of these lengths was calculated to evaluate lip symmetry. Results Of the patients treated at the Department of Plastic and Reconstructive Surgery at Kyungpook National University Chilgok Hospital, 11 (male-to-female sex ratio, 7:4) were diagnosed with Tessier number 7 craniofacial cleft. Concurrent congenital anomalies included skin tag, hemifacial microsomia, and cleft palate. The mean duration of follow-up was $78.273{\pm}72.219$ months and the mean ratio of the lengths of both sides of the lip was $1.048{\pm}0.071$. Scar widening occurred as a postoperative complication in some patients. No cases of wound infection, bleeding, or wound dehiscence occurred. Conclusions For the successful correction of macrostomia, plastic surgeons should consider both functional and aesthetic problems of the lip. Adequate repair of the orbicularis oris muscle, skin closure with Z-plasty, and medial overcorrection of the neo-oral commissure led to good results in our patients.

Surgical correction for Tessier number 7 craniofacial cleft using a medially overcorrected design

Jeong Yeop Ryu,Pil Seon Eo,Lulu Tian,Joon Seok Lee,이정우,최강영,양정덕,정호윤,조병채 대한성형외과학회 2019 Archives of Plastic Surgery Vol.46 No.1

Background Various surgical techniques have been used to correct Tessier number 7 craniofacial cleft, which involves macrostomia, ear deformity, and hemifacial microsomia. To achieve symmetrical and satisfactory results in patients with macrostomia, the authors performed a 1-mm medial overcorrection on the cleft side and evaluated the results of this procedure. Methods A retrospective medical record review of patients diagnosed with Tessier number 7 craniofacial cleft from March 1999 to February 2017 was performed. Using clinical photographs, outpatient clinic records, and operative records, information was recorded regarding concurrent congenital anomalies, postoperative complications, and follow-up. Using Photoshop CS2, the length of both sides of the lip was compared. The ratio of these lengths was calculated to evaluate lip symmetry. Results Of the patients treated at the Department of Plastic and Reconstructive Surgery at Kyungpook National University Chilgok Hospital, 11 (male-to-female sex ratio, 7:4) were diagnosed with Tessier number 7 craniofacial cleft. Concurrent congenital anomalies included skin tag, hemifacial microsomia, and cleft palate. The mean duration of follow-up was 78.273±72.219 months and the mean ratio of the lengths of both sides of the lip was 1.048±0.071. Scar widening occurred as a postoperative complication in some patients. No cases of wound infection, bleeding, or wound dehiscence occurred. Conclusions For the successful correction of macrostomia, plastic surgeons should consider both functional and aesthetic problems of the lip. Adequate repair of the orbicularis oris muscle, skin closure with Z-plasty, and medial overcorrection of the neo-oral commissure led to good results in our patients.

Omp16, a conserved peptidoglycan-associated lipoprotein, is involved in Brucella virulence in vitro

Feijie Zhi,Dong Zhou,Junmei Li,Lulu Tian,Guangdong Zhang,Yaping Jin,Aihua Wang 한국미생물학회 2020 The journal of microbiology Vol.58 No.9

Brucella, the bacterial agent of common zoonotic brucellosis, primarily infects specific animal species. The Brucella outer membrane proteins (Omps) are particularly attractive for developing vaccine and improving diagnostic tests and are associated with the virulence of smooth Brucella strains. Omp16 is a homologue to peptidoglycan-associated lipoproteins (Pals), and an omp16 mutant has not been generated in any Brucella strain until now. Very little is known about the functions and pathogenic mechanisms of Omp16 in Brucella. Here, we confirmed that Omp16 has a conserved Pal domain and is highly conserved in Brucella. We attempted to delete omp16 in Brucella suis vaccine strain 2 (B. suis S2) without success, which shows that Omp16 is vital for Brucella survival. We acquired a B. suis S2 Omp16 mutant via conditional complementation. Omp16 deficiency impaired Brucella outer membrane integrity and activity in vitro. Moreover, inactivation of Omp16 decreased bacterial intracellular survival in macrophage RAW 264.7 cells. B. suis S2 and its derivatives induced marked expression of IL-1β, IL-6, and TNF-α mRNA in Raw 264.7 cells. Whereas inactivation of Omp16 in Brucella enhanced IL-1β and IL-6 expression in Raw 264.7 cells. Altogether, these findings show that the Brucella Omp16 mutant was obtained via conditional complementation and confirmed that Omp16 can maintain outer membrane integrity and be involved in bacterial virulence in Brucella in vitro and in vivo. These results will be important in uncovering the pathogenic mechanisms of Brucella.

BK Knockout by TALEN-Mediated Gene Targeting in Osteoblasts: KCNMA1 Determines the Proliferation and Differentiation of Osteoblasts

Hei, Hongya,Gao, Jianjun,Dong, Jibin,Tao, Jie,Tian, Lulu,Pan, Wanma,Wang, Hongyu,Zhang, Xuemei Korean Society for Molecular and Cellular Biology 2016 Molecules and cells Vol.39 No.7

Large conductance calcium-activated potassium (BK) channels participate in many important physiological functions in excitable tissues such as neurons, cardiac and smooth muscles, whereas the knowledge of BK channels in bone tissues and osteoblasts remains elusive. To investigate the role of BK channels in osteoblasts, we used transcription activator-like effector nuclease (TALEN) to establish a BK knockout cell line on rat ROS17/2.8 osteoblast, and detected the proliferation and mineralization of the BK-knockout cells. Our study found that the BKknockout cells significantly decreased the ability of proliferation and mineralization as osteoblasts, compared to the wild type cells. The overall expression of osteoblast differentiation marker genes in the BK-knockout cells was significantly lower than that in wild type osteoblast cells. The BK-knockout osteoblast cell line in our study displays a phenotype decrease in osteoblast function which can mimic the pathological state of osteoblast and thus provide a working cell line as a tool for study of osteoblast function and bone related diseases.

BK Knockout by TALEN-Mediated Gene Targeting in Osteoblasts: KCNMA1 Determines the Proliferation and Differentiation of Osteoblasts

Xuemei Zhang,Hongya Hei,Jianjun Gao,Jibin Dong,Jie Tao,Lulu Tian,Wanma Pan,Hongyu Wang 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.7

Large conductance calcium-activated potassium (BK) channels participate in many important physiological functions in excitable tissues such as neurons, cardiac and smooth muscles, whereas the knowledge of BK channels in bone tissues and osteoblasts remains elusive. To investigate the role of BK channels in osteoblasts, we used transcription activator-like effector nuclease (TALEN) to establish a BK knockout cell line on rat ROS17/2.8 osteoblast, and detected the proliferation and mineralization of the BK-knockout cells. Our study found that the BK-knockout cells significantly decreased the ability of proliferation and mineralization as osteoblasts, compared to the wild type cells. The overall expression of osteoblast differentiation marker genes in the BK-knockout cells was significantly lower than that in wild type osteoblast cells. The BK-knockout osteoblast cell line in our study displays a phenotype decrease in osteoblast function which can mimic the pathological state of osteoblast and thus provide a working cell line as a tool for study of osteoblast function and bone related diseases.