The Association of SERPINE2 Gene with COPD in a Chinese Han Population

Aihua Wang,Wei Xiao,Yingqiu Yin,Ping Chen,Qiji Liu,Qinfeng Yu 연세대학교의과대학 2011 Yonsei medical journal Vol.52 No.6

Purpose: Polymorphisms of several candidate genes have been studied and associated with the development of chronic obstructive pulmonary disease (COPD). One such candidate is the SERPINE2 (Serpin peptidase inhibitor, clade E member 2) gene. Materials and Methods: To assess whether the SERPINE2 gene is associated with COPD in a Chinese Han population. Samples were collected from a Chinese Han population and analyzed for the association of single nucleotide polymor phisms (SNPs) or haplotypes of SERPINE2 gene with COPD in a case-control study. Three SNPs including rs840088 G/A in intron 1, rs1438831 A/G in 5’ upstream sequence and rs3795879 G/A in intron 3 were detected using the polymerase chain reaction (PCR)-based restriction fragment length polymorphism technique in 409 COPD subjects and 411 controls. Genotyping of the SREPINE2 polymorphisms at positions rs840088, rs1438831and rs3795879 was performed. Results: We found that none of the rs840088G/A, rs1438831G/A and rs3795879 G/A polymorphisms were associated with the disease. The p-values were 0.630, 0.208 and 0.398 respectively. Conclusion: Our data suggested that there was no significant association between SERPINE2 polymorphism and COPD susceptibility in the Chinese Han population.

An Efficient Algorithm for Performance Analysis of Multi-cell and Multi-user Wireless Communication Systems

( Aihua Wang ),( Jihua Lu ) 한국인터넷정보학회 2011 KSII Transactions on Internet and Information Syst Vol.5 No.11

Theoretical Bit Error Rate (BER) and channel capacity analysis are always of great interest to the designers of wireless communication systems. At the center of such analyses people are often encountered with a high-dimensional multiple integrals with quite complex integrands. Conventional Gaussian quadrature is inefficient in handling problems like this, as it tends to entail tremendous computational overhead, and the principal order of its error term increase rapidly with the dimension of the integral. In this paper, we propose a new approach to calculate complex multi-fold integrals based on the number theory. In contrast to Gaussian quadrature, the proposed approach requires less computational effort, and the principal order of its error term is independent of the dimension. The effectiveness of the number theory based approach is examined in BER and capacity analyses for practical systems. In particular, the results generated by numerical computation turn out in good match with that of Monte-Carlo simulations.

The Attenuation Mechanism and Live Vaccine Potential of a Low-Virulence Edwardsiella ictaluri Strain Obtained by Rifampicin Passaging Culture

Wang Shuyi,Hao Jingwen,Yang Jicheng,Zhang Qianqian,Li Aihua 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.2

The rifampicin-resistant strain E9-302 of Edwardsiella ictaluri strain 669 (WT) was generated by continuous passage on BHI agar plates containing increasing concentrations of rifampicin. E9-302 was attenuated significantly by 119 times to zebrafish Danio rerio compared to WT in terms of the 50% lethal dose (LD50). Zebrafish vaccinated with E9-302 via intraperitoneal (IP) injection at a dose of 1 × 103 CFU/fish had relative percentage survival (RPS) rates of 85.7% when challenged with wild-type E. ictaluri via IP 14 days post-vaccination (dpv). After 14 days of primary vaccination with E9-302 via immersion (IM) at a dose of 4 × 107 CFU/ml, a booster IM vaccination with E9-302 at a dose of 2 × 107 CFU/ml exhibited 65.2% RPS against challenge with wild-type E. ictaluri via IP 7 days later. These results indicated that the rifampicin-resistant attenuated strain E9-302 had potential as a live vaccine against E. ictaluri infection. A previously unreported amino acid site change at position 142 of the RNA polymerase (RNAP) β subunit encoded by the gene rpoB associated with rifampicin resistance was identified. Analysis of the whole-genome sequencing results revealed multiple missense mutations in the virulence-related genes esrB and sspH2 in E9-302 compared with WT, and a 189 bp mismatch in one gene, whose coding product was highly homologous to glycosyltransferase family 39 protein. This study preliminarily explored the molecular mechanism underlying the virulence attenuation of rifampicin-resistant strain E9-302 and provided a new target for the subsequent study of the pathogenic mechanism of E. ictaluri.

Rosa roxburghii Tratt juice inhibits NF-κB and increases IL-2 to alleviates the Foxp3-mediated Tregs imbalance in the peripheral blood of arseniasis patients

Qi Wang,Xiaolin Fang,Baofei Sun,Kai Zhu,Maolin Yao,Shaofeng Wei,Aihua Zhang 한국식품과학회 2024 Food Science and Biotechnology Vol.33 No.4

Arsenic can cause immune inflammation, which is the basis of arsenic-induced damage to multiple organs and systems. Forkhead box P3 (Foxp3)-labelled CD4+CD25+ regulatory T cells (Tregs) play an essential role in maintaining immune homeostasis. Nuclear factor-κb (NF-κB) and Interleukin-2 (IL-2) are critical regulators of Foxp3. Rosa roxburghii Tratt (RRT) is an edible medicinal plant with anti-inflammation effects. In this study, a control group (n = 41) and an arseniasis group (n = 209) were recruited, and screened subjects from the arseniasis patients for RRTJ (n = 46) or placebo (n = 43) to explore the possible mechanism by which RRT alleviates immune inflammation. The results indicated that RRTJ can inhibits NF-κB and increases IL-2, and alleviates the Foxp3-mediated Tregs imbalance in the peripheral blood of arseniasis patients. In summary, these findings suggest a novel intervention or therapeutic target for immune inflammation in arseniasis patients and provide new evidence that RRTJ inhibits immune inflammation.

Suppression and stability analysis of frequency coupling effect in grid‑connected inverters

Xiaohuan Wang,Aihua Yang,Yang Liu,Xiaojun Zhao 전력전자학회 2023 JOURNAL OF POWER ELECTRONICS Vol.23 No.6

With the proposal of the “dual carbon target” in China, the rapid development of renewable energy, mainly photovoltaic and wind power, has been promoted. However, a large amount of renewable energy connected to a grid results in its proportion in the power system going from a low state to a high state. Under a high proportion, the asymmetry of the control structure or parameters in the three-phase grid-connected inverter controller lead to a strong coupling relationship between the sub/super synchronous frequency components, or frequency coupling effect (FCE). This phenomenon can deteriorate the power quality of the inverter system, amplify the harm of frequency oscillation, and even cause system disassembly. To solve the above problems, a unified impedance model considering the FCE induced by the phase-locked loop (PLL), the current loop (CL), and the power outer loop (POL) is established. Based on the established output impedance model, a parameter optimization theoretical analysis method is designed considering the critical stability of the PLL bandwidth and the CL asymmetry degree. Meanwhile, an improved control strategy for the PLL and the POL is proposed. Experimental results show that the proposed parameter optimization and structure improvement strategies can effectively suppress the infl uence of frequency coupling and enhance system stability. Finally, simulation and experimental results verify the correctness of the theoretical analysis and the eff ectiveness of the proposed strategy.

Suberoylanilide hydroxamic acid suppresses axonal damage and neurological dysfunction after subarachnoid hemorrhage via the HDAC1/HSP70/TDP-43 axis

Luo Kui,Wang Zhifei,Zhuang Kai,Yuan Shishan,Liu Fei,Liu Aihua 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Increased focus has been placed on the role of histone deacetylase inhibitors as crucial players in subarachnoid hemorrhage (SAH) progression. Therefore, this study was designed to expand the understanding of SAH by exploring the downstream mechanism of the histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA) in SAH. The expression of TDP-43 in patients with SAH and rat models of SAH was measured. Then, western blot analysis, immunofluorescence staining, and transmission electron microscope were used to investigate the in vitro effect of TDP-43 on a neuronal cell model of SAH established by oxyhemoglobin treatment. Immunofluorescence staining and coimmunoprecipitation assays were conducted to explore the relationship among histone deacetylase 1 (HDAC1), heat shock protein 70 (HSP70), and TDP-43. Furthermore, the in vivo effect of HDAC1 on SAH was investigated in rat models of SAH established by endovascular perforation. High expression of TDP-43 in the cerebrospinal fluid of patients with SAH and brain tissues of rat models of SAH was observed, and TDP-43 accumulation in the cytoplasm and the formation of inclusion bodies were responsible for axonal damage, abnormal nuclear membrane morphology, and apoptosis in neurons. TDP-43 degradation was promoted by the HDAC1 inhibitor SAHA via the acetylation of HSP70, alleviating SAH, and this effect was verified in vivo in rat models. In conclusion, SAHA relieved axonal damage and neurological dysfunction after SAH via the HSP70 acetylation-induced degradation of TDP-43, highlighting a novel therapeutic target for SAH.

Operation Principle and Topology Structures of Axial Flux-Switching Hybrid Excitation Synchronous Machine

Liu, Xiping,Wang, Chen,Zheng, Aihua Journal of International Conference on Electrical 2012 Journal of international Conference on Electrical Vol.1 No.3

The operation principle of an axial flux-switching hybrid excitation synchronous machine (AFHESM) is analyzed and its topology structures are proposed in this paper. After some comprehensive analysis of the operation principle to axial flux electrical machine, flux-switching electrical machine and hybrid excitation electrical machine, the operation principle of AFHESM is given. Combined with some typical topological structures of hybrid excitation electrical machine, some possible topological structures are proposed and some comprehensive comparisons are carried out. The analysis results show that the stator-separated AFHESM has some advantages such as less AM turns, less impact on the demagnetization of PM, less magnetic flux-leakage and higher efficiency compared to other topologies.

DNA Vaccines Encoding Toxoplasma gondii Cathepsin C1 Induce Protection against Toxoplasmosis in Mice

Yali Han,Aihua Zhou,Gang Lu,Guanghui Zhao,Wenchao Sha,Lin Wang,Jingjing Guo,Jian Zhou,Huaiyu Zhou,Hua Cong,Shenyi He 대한기생충학열대의학회 2017 The Korean Journal of Parasitology Vol.55 No.5

Nattokinase Crude Extract Inhibits Hepatocellular Carcinoma Growth in Mice

( Yongmin Yan ),( Yanjing Wang ),( Jiali Qian ),( Sihui Wu ),( Yi Ji ),( Yanxiao Liu ),( Jian Zeng ),( Aihua Gong ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.8

Nattokinase (NK, E.C. 3.4.21.62) is a serine protease produced by Bacillus subtilis natto that shows promise for the treatment of thrombotic disease. In this study, we assessed the effects of NK on the development of hepatocellular carcinoma (HCC), a principal malignancy of the liver that causes morbidity and mortality worldwide. Crude extracts of NK (NCE) were isolated from fermentation medium by centrifugation and separated into three fractions (< 10 K, 100~30 K and >30K). Orthotopic HCC mouse models were established and NCE was administered by oral gavage. H&E staining was performed to examine the pathology of HCC livers. Immunohistochemistry and immunofluorescence were used to evaluate FOXM1, CD31, CD44 and vimentin expression in the liver. Compared to PBS groups, NCE increased the survival rates of HCC-bearing mice to 31% and decreased ascites. Low-intensity ultrasound imaging showed that the hypoechoic mass area was lower in NCE-treated mice and that tumor growth significantly decreased. IHC staining showed that the expression of FOXM1 was inhibited by NCE treatment. Immunofluorescence results revealed lower levels of CD31, CD44 and vimentin in the NCE groups. Taken together, these data demonstrate that NCE from Bacillus subtilis natto improves survival and inhibits tumor growth in HCC mice.

De novo Assembly and Analysis of Amur Sturgeon (Acipenser schrenckii) Transcriptome in Response to Mycobacterium Marinum Infection to Identify Putative Genes Involved in Immunity

( Qianqian Zhang ),( Xiehao Wang ),( Defeng Zhang ),( Meng Long ),( Zhenbing Wu ),( Yuqing Feng ),( Jingwen Hao ),( Shuyi Wang ),( Qian Liao ),( Aihua Li ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.8

Fish mycobacteriosis is a common bacterial disease in many species of freshwater and marine fish and has caused severe loss of fish production. Mycobacterium marinum has been the most prevalent pathogen observed in several outbreaks of mycobacteriosis of farmed sturgeons in China. However, the immune responses and pathology of sturgeons in mycobacterial infection are rarely studied. Therefore, we used the Illumina RNA-seq method to analyze the transcriptome profile of Acipenser schrenckii challenged with Mycobacterium marinum. To begin, 168,220 non-redundant contigs were acquired from the infection and control groups, and among these, 33,225 contigs have acquired annotations. A total of 4,043 differently expressed (DE) contigs between the two groups were identified, and among these, 2479 were upregulated and 1564 were down-regulated in the infected fish. A total of 1,340 DE contigs with acquired annotations in KEGG were enriched for 124 pathways including the TNF signaling pathway, and the Toll-like receptor signaling pathway. The roles of DE genes involved in significant pathways and other processes were discussed. The 2,209 DE contigs that have yet to acquire proper annotation may represent candidate genes associated with infection in sturgeons and are expected to serve as immunogenetic resources for further study. To our best knowledge, this is the first transcriptome study on sturgeons under bacterial infection.