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Array-based Nano-amplification Technique Was Applied in Detection of Hepatitis E Virus
Liu, Hui-Hui,Cao, Xuan,Yang, Yong,Liu, Ming-Gui,Wang, Ye-Fu Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.3
A rapid method for the detection of Hepatitis E Virus (HEV) was developed by utilizing nano-gold labeled oligonucleotide probes, silver stain enhancement and the microarray technique. The 5'-end -$NH_2$ modified oligonucleotide probes were immobilized on the surface of the chip base as the capture probe. The detection probe was made of the 3'-end -SH modified oligonucleotide probe and nano-gold colloid. The optimal concentrations of these two probes were determined. To test the detection sensitivity and specificity of this technique, a conservative fragment of the virus RNA was amplified by the RT-PCR/PCR one step amplification. The cDNA was hybridized with the capture probes and the detection probes on microarray. The detection signal was amplified by silver stain enhancement and could be identified by naked eyes. 100 fM of amplicon could be detected out on the microarray. As the results, preparation of nano-gold was improved and faster. Development time also was shortened to 2 min. Thus, considering high efficiency, low cost, good specificity and high sensitivity, this technique is alternative for the detection of HEV.
( Gui-qing Liu ),( Xue-hong Qiu ),( Li Cao ),( Ri-chou Han ) 한국균학회 2018 Mycobiology Vol.46 No.4
The entomopathogenic fungus Cordyceps militaris is a valuable medicinal ascomycete, which degenerates frequently during subsequent culture. To avoid economic losses during industrialized production, scratching stimuli of mycelia was introduced to improve the fruiting body production. The present results indicated that higher yields and biological efficiency were obtained from two degenerate strains (YN1-14 and YN2-7) but not from g38 (an insertional mutant in Rhf1 gene with higher yields and shorter growth periods). Furthermore, the growth periods of the fruiting bodies were at least 5 days earlier when the mycelia were scratched before stromata differentiation. Three ROS-scavenging genes including Cu/Zn superoxide dismutase (CmSod1), Glutathione peroxidase (CmGpx), and Catalase A (CmCat A) were isolated and their expression profiles against scratching were determined in degenerate strain YN1-14 and mutant strain g38. At day 5 after scratching, the expression level of CmGpx significantly decreased for strain g38, but that of CmSod1 significantly increased for YN1-14. These results indicated that scratching is an effective way to promote fruiting body production of degenerate strain, which may be related at least with Rhf1 and active oxygen scavenging genes.
A Novel Interlaced-helical Wrapping Yarn with Negative Poisson’s Ratio
Sai Liu,Zhaoqun Du,Kaifang Xie,Gui Liu,Shu Yang 한국섬유공학회 2018 Fibers and polymers Vol.19 No.11
The two-component complex yarn with helical wrapping structure has been reported with auxetic behavior and unique performance, however, the residual torque could lead to the slippage of the wrap component from the core in the natural state and poor stability and applicability. Therefore, this paper aims to introduce a novel interlaced-helical wrapping yarn with negative Poisson’s ratio, stable structure, practicability and continuous preparation. The differences in geometry and auxetic behavior between helical structure and interlaced-helical structure were presented and analyzed. Both the structure stability and auxetic effect of the interlaced-helical complex yarn have advantages over the helical wrap yarn with same materials and initial wrap angle. It can be shown that the interlaced-helical wrap yarns with lower initial wrap angle and bigger tensile modulus of the stiff yarns will produce better auxetic effect. Besides, the deformation mechanism and the manufacturing process were described in detail. Several kinds of yarns have been fabricated to discuss the effect of initial wrap angle and tensile modulus on the Poisson’s ratio of the structure. The maximum value of Poisson’s ratio for the yarns manufactured was -2.8. It is also expected that auxetic yarns with novel braided structure can prompt the applications of auxetic textiles.
Effect of Temperature on Resistivity of CFRP Materials with Added Carbon Powder or Nano-silica
Dan Liu,Jie Yin,Rong-gui Liu,Shu Ping,Hui-guang Yin,Gui-hua Xie 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.4
This paper presents an experimental investigation on the effect of temperature on resistivity of Carbon Fiber Reinforced Polymer (CFRP) materials. A series of tests were conducted on three types of CFRP materials, namely pure CFRP material, CFRP with carbon powder (4% in weight) and CFRP with nano-silica (4% in weight). Test results showed that adding carbon powder into the epoxy resin decreases the initial electric resistance R0 and initial volume resistivity ρ0 while adding nano-silica increases R0 and ρ0 compared to pure CFRP material. Preheating cycle test results showed that the volume resistivity of all three types of specimens linearly increases with increasing temperature. CFRP with added nano-silica exhibits higher temperature sensitivity than CFRP with added carbon powder compared to the lowest temperature sensitivity for pure CFRP material. In addition, temperature cycle test results showed that CFRP specimens have approximately stable values of volume resistivity. Both CFRP specimens with added carbon powder or nano-silica exhibit a recognizable trend of first decrease and then increase in volume resistivity with increasing temperature both during heating and cooling cycles. CFRP with added carbon powder mainly shows Negative Temperature Coefficient (NTC) effect in the temperature range of −40 to 40oC and Positive Temperature Coefficient (PTC) effect from 40 to 80oC. CFRP with added nano-silica mainly exhibits PTC effect in the temperature domain of −15 to 80oC and NTC effect from −40 to −15oC. A mathematical-physical model with respect to the thermal effect was presented based on the Eshelby-Mori-Tanaka (EMT) approach and mesomechanics method. The results obtained with the model agree well with the test results considering the temperature domain of PTC effect, which indicates that the proposed model is effective in characterizing the variation of fractional change in resistance (ΔR/R0) at varying temperature.
Shuang Ping Liu,Rui Xia Liu,Jian Mao,Liang Zhang,Zhongyang Ding,Zhenghua Gu,Gui-Yang Shi 한국생물공학회 2016 Biotechnology and Bioprocess Engineering Vol.21 No.1
The aproteinogenic amino acid, L-phenylglycine, is an important side chain building block for some drugs. It would be of great commercial and environmental value to biocatalyse L-phenylalanine to L-phenylglycine, and thus replace the organic synthesis method. To produce Lphenylglycine from L-phenylalanine, an L-phenylglycine aminotransferase was screened and characterized. HpgTAO showed high homology to α-aminoadipate aminotransferase. The L-phenylalanine binding site was near the residues S26, R401, N201, and G46 in HpgTAO, and L-phenylalanine formed a hydrogen bond with Asn20, which was similar to the substrate binding mechanism of α-aminoadipate aminotransferase. HpgTAO showed increased activity in alkalescent environment below 40°C. The kinetic analysis showed that L-phenylalanine had the highest affinity to HpgTAO, which ensured the recycle biosynthesis of Lphenylglycine from L-phenylalanine. To date, it was the only aminotransferase using L-phenylalanine as an optimal amino donor. The L-phenylglycine biocatalysis operon was also constructed by co-expressing the hmaS, hmo and hpgT by a single plasmid. The first in vitro conversion of Lphenylalanine to L-phenylglycine was achieved by directly using the L-phenylalanine fermentation broth as the raw material.