Reducing Nogo-B Improves Hepatic Fibrosis by Inhibiting BACe1-Mediated Autophagy

Gao LiLi,Zhuang YingJie,Liu ZhengYi 한국조직공학과 재생의학회 2024 조직공학과 재생의학 Vol.21 No.5

Background: Hepatic fibrosis (HF) is a histopathological change in the process of long-term liver injury caused by cytokine secretion and internal environment disturbance, resulting in excessive liver repair and fiber scar. Nogo-B protein is widely distributed in peripheral tissues and organs and can regulate the migration of endothelial cells by activating TGF-β1 in vascular remodeling after injury. Nogo-B has been shown to promote organ fibrosis. This study was to determine the role of Nogo-B in HF. Methods: An HF model was built by intraperitoneal injections with 20% carbon tetrachloride. Localization of Nogo-B was detected by FISH. The interaction between Nogo-B and BACE1 was confirmed by Co-IP. Autophagy flux was analyzed using tandem mRFP-GFP-LC3 fluorescence microscopy, electron microscopy, and western blotting. Detection of serum AST and ALT and H&E staining were utilized to detect the degree of liver injury. The HF was evaluated by Masson trichromatic staining. RT-qPCR, western blotting, and immunofluorescence were employed to detect relevant indicators. Results: Reducing Nogo-B suppressed AST and ALT levels, the accumulation of collagen I and α-SMA, and expressions of pro-fibrotic genes in mouse liver. BACE1 was a potential downstream target of Nogo-B. Nogo-B was upregulated in TGF-β1-activated hepatic stellate cells (HSCs). Knocking down Nogo-B caused the downregulation of pro-fibrotic genes and inhibited viability of HSCs. Nogo-B knockdown prevented CCL4-induced fibrosis, accompanied by downregulation of extracellular matrix. Nogo-B inhibited HSC autophagy and increased lipid accumulation. BACE1 knockdown inhibited HSC autophagy and activation in LX-2 cells. Conclusion: Nogo-B knockdown prevents HF by directly inhibiting BACe1-mediated autophagy. Background: Hepatic fibrosis (HF) is a histopathological change in the process of long-term liver injury caused by cytokine secretion and internal environment disturbance, resulting in excessive liver repair and fiber scar. Nogo-B protein is widely distributed in peripheral tissues and organs and can regulate the migration of endothelial cells by activating TGF-β1 in vascular remodeling after injury. Nogo-B has been shown to promote organ fibrosis. This study was to determine the role of Nogo-B in HF. Methods: An HF model was built by intraperitoneal injections with 20% carbon tetrachloride. Localization of Nogo-B was detected by FISH. The interaction between Nogo-B and BACE1 was confirmed by Co-IP. Autophagy flux was analyzed using tandem mRFP-GFP-LC3 fluorescence microscopy, electron microscopy, and western blotting. Detection of serum AST and ALT and H&E staining were utilized to detect the degree of liver injury. The HF was evaluated by Masson trichromatic staining. RT-qPCR, western blotting, and immunofluorescence were employed to detect relevant indicators. Results: Reducing Nogo-B suppressed AST and ALT levels, the accumulation of collagen I and α-SMA, and expressions of pro-fibrotic genes in mouse liver. BACE1 was a potential downstream target of Nogo-B. Nogo-B was upregulated in TGF-β1-activated hepatic stellate cells (HSCs). Knocking down Nogo-B caused the downregulation of pro-fibrotic genes and inhibited viability of HSCs. Nogo-B knockdown prevented CCL4-induced fibrosis, accompanied by downregulation of extracellular matrix. Nogo-B inhibited HSC autophagy and increased lipid accumulation. BACE1 knockdown inhibited HSC autophagy and activation in LX-2 cells. Conclusion: Nogo-B knockdown prevents HF by directly inhibiting BACe1-mediated autophagy.

Research on Consistency Maintenance of Right Management Operations in Real time Collaboration Environments

Liping Gao,Qiongqiong Fu,Lili Gao,Yuben Zhang,Qingkui Chen 보안공학연구지원센터 2014 International Journal of Database Theory and Appli Vol.7 No.3

Immunomodulatory effects of Clostridium butyricum on human enterocyte-like HT-29 cells

Gao Quanxin,Wang Jinbo,Qi Lili,Wu Tianxing,Xia Tingting 한국통합생물학회 2013 Animal cells and systems Vol.17 No.2

Oral administration of Clostridium butyricum as probiotics is increasingly gaining importance in the treatment of intestinal inflammations and improvement of animal performance. The mechanisms of host cell receptor recognition of C. butyricum and downstream immune signaling pathways leading to this beneficial event, however, remain unclear. In this study, C. butyricum was investigated for its capability to influence the innate immune response of HT-29 cells to explore its mechanism of action. Our data showed that C. butyricum was able to stimulate toll-like receptor 2 (TLR2) production at mRNA level; however, TLR4, TLR5, TLR9, and myeloid differentiation primary response protein 88 (MyD88) transcription levels remain unaltered. The nuclear factor kB (NF-kB), interleukin-8 (IL-8), and tumor necrosis factor alpha (TNF-a) levels in response to C. butyricum were significantly increased, indicating that HT-29 cells were sensitised by C. butyricum. It is the first time that our findings have showed the specific signaling pathways in HT-29 cells stimulated by C. butyricum, which, at least in part, can help to explain the beneficial properties of C. butyricum.

High level of LncRNA MAPKAPK5-AS1 predicts poor prognosis and contributes to the malignant proliferation and EMT of non-small cell lung cancer via sponging miR-490-3p from HMGB2

Miao Jidong,Gao Yang,Guan Wenqiang,Yu Xiaolin,Wang Yong,Jiang Ping,Yang Lili,Xu Lun,You Wei 한국유전학회 2023 Genes & Genomics Vol.45 No.5

Background Patients with non-small cell lung cancer (NSCLC) show a low survival rate, owing to the lack of early diagnostic method and high invasiveness. Long non-coding RNA MAPKAPK5-AS1 that regulates tumor genesis and progression through multiple signals, is upregulated and involved in the growth and apoptosis in lung adenocarcinoma (LUAD). Objective To investigate whether MAPKAPK5-AS1 affected the malignant progression of NSCLC. Methods The levels of MAPKAPK5-AS1, miR-490-3p and HMGB2 in lung cancer were first analyzed through StarBase website, and confirmed by a quantitative reverse transcriptase-PCR (qRT-PCR) assay. The biological functions of NSCLC cells were examined by CCK-8, 5-ethynyl-2ʹ-deoxyuridine (EdU) and flow cytometry assays. The potential binding sequences lncRNA-miRNA and miRNA-mRNA were predicted by StarBase software and verified via dual luciferase reporter experiment. The effects of MAPKAPK5-AS1 on tumor growth were evaluated in a xenografted mice model. Results The expression of MAPKAPK5-AS1 was upregulated in tumor tissues from NSCLC patients. Patients with high expression of MAPKAPK5-AS1 had higher tumor size, advanced TNM stage, higher incidence of lymph node and distant metastasis, and shorter overall survival. Knockdown of MAPKAPK5-AS1 inhibited the proliferation, induced apoptosis and blocked epithelial mesenchymal transformation (EMT) of NSCLC cells. Mechanically, MAPKAPK5-AS1 could upregulate the HMGB2 level in NSCLC cells through competitively binding to miR-490-3p. MiR-490-3p inhibitor reversed the roles of MAPKAPK5-AS1 knockdown on tumor cell proliferation, apoptosis and EMT. Also, HMGB2 knockdown suppressed tumor cell malignant phenotypes. Furthermore, interference of MAPKAPK5-AS1 slowed NSCLC tumor growth in vivo. Conclusion Knockdown of MAPKAPK5-AS1 inhibited the aggressive tumor phenotypes through miR-490-3p/HMGB2 axis in NSCLC. MAPKAPK5-AS1/miR-490-3p/HMGB2 might be potential biomarkers or therapeutic targets for NSCLC.

Numerical Investigation of the Turbulent Cavitating Flow over Submerged Bodies

Hong Feng,Gao Zhenjun,Liu Lili,Yuan Jianping 한국유체기계학회 2018 International journal of fluid machinery and syste Vol.11 No.1

A numerical method for the calculation of turbulent cavitating flow over submerged objects is proposed in present work. Cavitation is modeled via a single-fluid cavitation model which is derived based on a truncated form of the Rayleigh-Plesset equation and the mixture multiphase theory. The approach has been implemented by user-define function which is widely used in ANSYS FLUENT. Detailed results are presented for sheet cavitation over a submerged hemispherical object in a wide range of cavitation numbers and the cloud cavitation around a Clark-Y hydrofoil. In particular, for the hemispherical body, we compared the surface pressure distribution with experimental data which was available in literature. Later the cloud cavitation structure and its effect on the forces of the hydrofoil were studied. The comparisons between the simulating and experimental results show that present numerical approach has good capability to predict the surface pressure coefficient and the pulsation frequency at cavitation number σ=0.4, 0.55 and 0.65 of the hemispherical body under cavitation conditions. Meanwhile, for the hydrofoil, the proposed approach is sufficiently robust to predict the characteristics of the time-averaged lift and drag coefficients and the evolution of the cloud cavity with time.

Simultaneously photocatalytic removal of Cr(VI) and metronidazole by asynchronous cross-linked modified sodium alginate

Shaojie Wu,Mingyang Li,Lili Xin,Hongming Long,Xiangpeng Gao 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.116 No.-

Conventional photocatalysts for environmental applications are mainly transitional metal compounds orprecious metal nanoparticles, which suffer from weak pollutant adsorption performance, thus limitedtheir applications for high concentrated wastewaters. In this study, asynchronous cross-linked triethylenetetraminemodified sodium alginate (CFTS) was used as photocatalyst for the simultaneouslycatalytic degradation of Cr(VI) and metronidazole (MNZ). Experimental results suggested that asynchronouscross-linking and triethylenetetramine modification have dramatically enhanced the chemicalstability as well as the adsorption and photocatalytic behavior of the material. The grafted amino functionalgroups and cross-linked Fe3+ ions can promote the rapid separation and transfer of electrons, thusreducing the recombination rate of photo-generated electron-hole pairs. The optimum removal rate of Cr(VI) has reached 99.5 % and MNZ can be completely removed within 20 and 30 min at pH 1.0 under UVlight irradiation, respectively. The removal mechanism of Cr(VI) by CFTS is the synergistic effect of electrostaticadsorption and photocatalytic reduction, while photocatalytic oxidation dominated the degradationprocess of MNZ. Moreover, the removal efficiency can be enhanced in the binary solution ofthese two pollutants due to the utilization of photo generated electron-hole pairs, which provides newpathways in the treatment of complicated pharmaceutical and domestic sewage wastewaters.

Effects of chronic alcohol exposure on ischemia–reperfusion-induced acute kidney injury in mice: the role of β-arrestin 2 and glycogen synthase kinase 3

Lihua Wang,Yifei Zhu,Lili Wang,Jingjing Hou,Yongning Gao,Lei Shen,Jingyu Zhang 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

Little is known about the effects of chronic alcohol intake on the outcome of acute kidney injury (AKI). Hence, we examined the effects of chronic alcohol intake on the development of renal fibrosis following AKI in an animal model of bilateral renal ischemia–reperfusion (IR) injury. We first found that chronic alcohol exposure exacerbated bilateral IR-induced renal fibrosis and renal function impairment. This phenomenon was associated with increased bilateral IR-induced extracellular matrix deposition and an increased myofibroblast population as well as increased bilateral IR-induced expression of fibrosis-related genes in the kidneys. To explore the mechanisms underlying this phenomenon, we showed that chronic alcohol exposure enhanced β-arrestin 2 (Arrb2) expression and Akt and glycogen synthase kinase-3 (GSK3)β activation in the kidneys. Importantly, pharmacological GSK3 inhibition alleviated bilateral IR-induced renal fibrosis and renal function impairment. Furthermore, we demonstrated that Arrb2− / − mice exhibited resistance to IR-induced renal fibrosis and renal function impairment following chronic alcohol exposure, and these effects were associated with attenuated GSK3β activation in the kidneys. Taken together, our results suggest that chronic alcohol exposure may potentiate AKI via β-arrestin 2/Akt/GSK3β-mediated signaling in the kidney.

The Diet Imbalance and Its Corrective Action in Chinese Athletes

Yang, ZeYi,Zhou, Lili,Yi, Muqing,Wang, Qirong,Gao, Hong,Xu, BaoHua,Qiao, Li,Chen, Jidi,Huang, Yuan,He, Cong,Wu, Wei,Sun, Nan,Chen, Rui 한국체육무용국제교류학회 2001 한국체육무용국제교류학회지 Vol.7 No.-

Diet is the most important substantial base to ensure athletes nutrients needs and maintain the performance. It is important for the Olympic Gold Medal Strategy. An athlete is like a racing car. Therere two key factors for the car to run faster: the engine and the gasoline. The physical components decide the engines highest power while the diet is like the gasoline the better quality of the gasoline is, the faster the urns. It is inconsistent between the importance of diet for competitive sports and the ubiquity of diet imbalance. It will influence the training efficiency and the recovery after training for a long time, and consequently hinder the improvement of the performance.

A Novel Image Reconstruction Algorithm Based on Compressed Sensing for Electrical Capacitance Tomography

Chen Deyun,Li Zhiqiang,Gao Ming,Wang Lili,Yu Xiaoyang 보안공학연구지원센터(IJSIP) 2013 International Journal of Signal Processing, Image Vol.6 No.4

Research on Partial Discharge Pattern Recognition in GIS Based on EFPI Sensor

Wang Zan,Liu Zhongquan,Qiao Lili,Qian Dingdong,Chen Zhongxian,Gao Chaofei,Wang Wei 대한전기학회 2024 Journal of Electrical Engineering & Technology Vol.19 No.1

An EFPI fber optic ultrasonic sensor can be used for the detection and pattern recognition of partial discharge ultrasonic signals in Gas Insulated Switchgear. Compared with traditional piezoelectric sensors, it has many advantages, such as high sensitivity and strong anti-interference ability. Based on this, four typical PD models of the tip, metal particles, suspension and creeping surface were set up in the GIS cavity flled with 0.4 MPa SF6 gas, and the EFPI sensor was innovatively used to detect the discharge ultrasonic signal and extract the single ultrasonic pulse signal. The waveform features form a feature parameter database, and the probabilistic neural network algorithm and the support vector machine algorithm are used for pattern recognition and comparative analysis, respectively. The ultrasonic signal detected by the EFPI sensor has prominent features. On the basis of extracting the feature parameters, the two pattern recognition algorithms can achieve an average recognition rate of more than 85%, and the recognition efect of the support vector machine is better than that of the probabilistic neural network