SPON2 Promotes M1-like Macrophage Recruitment and Inhibits Hepatocellular Carcinoma Metastasis by Distinct Integrin–Rho GTPase–Hippo Pathways

Zhang, Yan-Li,Li, Qing,Yang, Xiao-Mei,Fang, Fang,Li, Jun,Wang, Ya-Hui,Yang, Qin,Zhu, Lei,Nie, Hui-Zhen,Zhang, Xue-Li,Feng, Ming-Xuan,Jiang, Shu-Heng,Tian, Guang-Ang,Hu, Li-Peng,Lee, Ho-Young,Lee, Su-J American Association for Cancer Research 2018 Cancer research Vol.78 No.9

<P>Matricellular protein SPON2 acts as an HCC suppressor and utilizes distinct signaling events to perform dual functions in HCC microenvironment.</P><P>Tumor-associated macrophages (TAM) represent key regulators of the complex interplay between cancer and the immune microenvironment. Matricellular protein SPON2 is essential for recruiting lymphocytes and initiating immune responses. Recent studies have shown that SPON2 has complicated roles in cell migration and tumor progression. Here we report that, in the tumor microenvironment of hepatocellular carcinoma (HCC), SPON2 not only promotes infiltration of M1-like macrophages but also inhibits tumor metastasis. SPON2-α4β1 integrin signaling activated RhoA and Rac1, increased F-actin reorganization, and promoted M1-like macrophage recruitment. F-Actin accumulation also activated the Hippo pathway by suppressing LATS1 phosphorylation, promoting YAP nuclear translocation, and initiating downstream gene expression. However, SPON2-α5β1 integrin signaling inactivated RhoA and prevented F-actin assembly, thereby inhibiting HCC cell migration; the Hippo pathway was not noticeably involved in SPON2-mediated HCC cell migration. In HCC patients, SPON2 levels correlated positively with prognosis. Overall, our findings provide evidence that SPON2 is a critical factor in mediating the immune response against tumor cell growth and migration in HCC.</P><P><B>Significance:</B> Matricellular protein SPON2 acts as an HCC suppressor and utilizes distinct signaling events to perform dual functions in HCC microenvironment.</P><P><B>Graphical Abstract:</B> http://cancerres.aacrjournals.org/content/canres/78/9/2305/F1.large.jpg. <I>Cancer Res; 78(9); 2305–17. ©2018 AACR</I>.</P><P><B>Graphical Abstract</B></P><P> [Figure]</P>

Cryopreservation of in Vitro Shoot Tips of Banana by Vitrification and Its Regeneration

Li Ming Wu,Ji Wu Zeng,Shu Ang Peng,Gan Jun Yi,Bi Rong Zhou,Yuan Li Wu 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Topology and thickness optimization of an indenter under stress and stiffness constraints

Ang Li,ChuSheng Liu,ShiZhe Feng 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.1

Indenter is one of the core components of a waste compression station. In this paper, a new layout free from the existing structure for indenter with lighter weight and good performance is presented. The design procedure includes two steps: Topology optimization and thickness optimization. In topology optimization process, a creative layout of indenter is obtained using Solid isotropic microstructures with penalization (SIMP) method. In thickness optimization process, based on topology optimization results, a design review was conducted, and a revised model was created which addresses all structural and manufacturability concerns. Shape and size optimization was then performed in the detailed design stage to further minimize the mass while meeting the stiffness and stress targets. Finally, an optimized model for the indenter is obtained. From the finite element analysis, it can be seen that there is a 31.8 % reduction in total mass while the performance increased compared with the original model.

제2형 당뇨병 환자의 기능적 헬스 리터러시가 주관적 건강에 미치는 영향

원앙리(Ang Li Won),유승현(Seung Hyun Yoo),유명순(Myoung Soon You) 한국보건교육건강증진학회 2014 보건교육건강증진학회지 Vol.31 No.3

Objectives: This study was performed to identify factors related to perceived health status among patients with type 2 diabetes. Methods: This is cross-sectional observational study. Respondents were 106 visitors in an outpatient diabetes clinic of a university hospital. Self-report questionnaire which included general information inquiry, diabetes-related, sociopsychological factors, functional health literacy and perceived health status was used for this study. The data was analyzed by using descriptive statistics, independent simple t-test, one-way ANOVA, and hierarchical multiple linear regression. All analysis were conducted using SAS 9.3. Results: Among the respondents, 43.4% engaged in poorly perceived health status. After adjusting for control variables, functional health literacy is significantly related to perceived health status(β=0.095, p=0.016). Conclusion: Independent of diabetes-related, sociopsychological factors, higher functional health literacy is associated with better perceived health status of patients with type 2 diabetes. In order to improve perceived health status in the type 2 diabetes patients, it is necessary to develop strategy to enhance the functional health literacy.

자연어 처리를 위한 트위터 감정 분석

이앙 ( Ang Li ),조인휘 ( Inwhee Joe ) 한국정보처리학회 2022 한국정보처리학회 학술대회논문집 Vol.29 No.2

인터넷 시대에 소셜 미디어는 사람들의 삶에 완전히 침투했다. 많은 사용자 기반을 보유한 성숙한 온라인 플랫폼 중 하나인 Twitter를 통해 사용자는 최신 뉴스, 삶의 경험 및 흥미로운 삶의 이야기를 독립적으로 게시할 수 있다. 하지만 때론 부정적인 뉘앙스를 풍기며 기업이나 개인의 브랜드에 영향을 미치며 이익을 훼손하는 경우가 있기 때문에 욕설을 식별해 트위터 발신을 차단할 필요가 있다. 이 기사의 가장 큰 혁신은 Twitter 데이터를 사용하여 다양한 방법을 동시에 비교한다는 것입니다. 더 많은 데이터를 처리할수록 딥 러닝을 시도하면 좋은 결과를 얻을 수 있다. Transformer 분류기를 통합하여 최상의 결과를 얻었다

Treatment of Periodontal Bone Defects with Stem Cells from Inflammatory Dental Pulp Tissues in Miniature Swine

Ye Li,Xi Nan,Tian-Yu Zhong,Tong Li,Ang Li 한국조직공학과 재생의학회 2019 조직공학과 재생의학 Vol.16 No.2

BACKGROUND: Containing a certain proportion of mesenchymal stem cells, inflammatory dental tissue showed great tissue regeneration potential in recent years. However, whether it is applicable to promote tissue regeneration in vivo remains to be elucidated. Therefore, we evaluated the feasibility of stem cells from inflammatory dental pulp tissues (DPSCs-IPs) to reconstruct periodontal defects in miniature pigs. METHODS: The autologous pig DPSCs-IPs were first cultured, appraised and loaded onto b-tricalcium phosphate (b- TCP). The compounds were then engrafted into an artificially-created periodontal defect. Three months later, the extent of periodontal regeneration was evaluated. Clinical examination, radiological examination and immunohistochemical staining were used to assess periodontal regeneration. RESULTS: The data collectively showed that DPSCs-IPs from miniature pigs expressed moderate to high levels of STRO-1 and CD146 as well as low levels of CD34 and CD45. DPSCs-IPs have osteogentic, adipogenic and chondrogenic differentiation abilities. DPSCs-IPs were engrafted onto b-TCP and regenerated bone to repair periodontal defects by 3 months’ post-surgical reconstruction. CONCLUSION: Autologous DPSCs-IPs may be a feasible means of periodontal regeneration in miniature pigs.

Multi-omics data driven analysis establishes reference codon biases for synthetic gene design in microbial and mammalian cells

Ang, K.S.,Kyriakopoulos, S.,Li, W.,Lee, D.Y. Academic Press 2016 Methods Vol.102 No.-

In this study, we analyzed multi-omics data and subsets thereof to establish reference codon usage biases for codon optimization in synthetic gene design. Specifically, publicly available genomic, transcriptomic, proteomic and translatomic data for microbial and mammalian expression hosts, Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris and Chinese hamster ovary (CHO) cells, were compiled to derive their individual codon and codon pair frequencies. Then, host dependent and -omics specific codon biases were generated and compared by principal component analysis and hierarchical clustering. Interestingly, our results indicated the similar codon bias patterns of the highly expressed transcripts, highly abundant proteins, and efficiently translated mRNA in microbial cells, despite the general lack of correlation between mRNA and protein expression levels. However, for CHO cells, the codon bias patterns among various -omics subsets are not distinguishable, forming one cluster. Thus, we further investigated the effect of different input codon biases on codon optimized sequences using the codon context (CC) and individual codon usage (ICU) design parameters, via in silico case study on the expression of human IFNγ sequence in CHO cells. The results supported that CC is more robust design parameter than ICU for improved heterologous gene design.

Impact of Energy Consumption, FDI and Trade Openness on Carbon Emissions in lvory Coast

Ange Aurore KADI,Liang LI,David Dauda LANSANA,Joseph FUSEINI 한국유통과학회 2024 Asian Journal of Business Environment (AJBE) Vol.14 No.3

Purpose: The study focuses on the impact of Foreign Direct Investment (FDI), trade openness, and energy consumption on carbon dioxide emissions in the Ivory Coast. It aims to quantitatively evaluate the effects of FDI, energy consumption, and trade openness on CO₂ emissions in Ivory Coast. Research design, data, and methodology: The research uses an econometric framework and the Autoregressive Distributed Lag (ARDL) model to analyze time-series data from 1980 to 2021 between these factors. Results: The analysis revealed that FDI significantly impacts the carbon dioxide emissions, FDI showed a negative impact on carbon emissions in the long-run equilibrium term. Also, energy consumption impacted CO₂ emissions in the long-run equilibrium term. Conclusion: To mitigate the upsurge of CO₂ emissions in the Ivorian context, concrete policy, including enactment and adherence to strict environmental regulations, adoption and prioritization of eco-friendly products and technologies, and investment in renewable energy infrastructure are recommended. The study contributes to the global discussion on sustainable development by offering a model for similar assessments in other emerging nations facing simultaneous economic growth and environmental conservation challenges.

Fluctuating wind field analysis based on random Fourier spectrum for wind induced response of high-rise structures

Li Lin,A.H.S. Ang,Dan-dan Xia,Hai-tao Hu,Huai-feng Wang,Fu-qiang He 국제구조공학회 2017 Structural Engineering and Mechanics, An Int'l Jou Vol.63 No.6

An accurate calculation of the stochastic wind field is the foundation for analyzing wind-induced structure response and reliability. In this research, the spatial correlation of structural wind field was considered based on the time domain method. A method for calculating the stochastic wind field based on cross stochastic Fourier spectrum was proposed. A flowchart of the proposed methodology is also presented in this study to represent the algorithm and workflow. Along with the analysis of regional wind speed distribution, the wind speed time history sample was calculated, and the efficiency can therefore be verified. Results show that the proposed method and programs could provide an efficient simulation for the wind-induced structure response analysis, and help determine the related parameters easily.

A self-powered β-Ga2O3/CsCu2I3 heterojunction photodiode responding to deep ultraviolet irradiation

Gao Ang,Jiang Weiyu,Ma Guoliang,Liu Zeng,Li Shan,Yan Zuyong,Sun Weiming,Zhang Shaohui,Tang Weihua 한국물리학회 2022 Current Applied Physics Vol.33 No.-

In this paper, a lead-free halide perovskite CsCu2I3 film with high stability was prepared by the anti-solvent assisted crystallization method. Then, we coupled it with Ga2O3 to prepare a corresponding heterojunction deep ultraviolet (UV) photodetector. After testing, we concluded that the photodetector is sensitive to 254 nm UV light. The photodetector has good reproducibility, and has an ultra-high photo-to-dark current ratio (PDCR) of more than 105. In addition, under a bias of 10 V and an illuminated intensity of 200 μW/cm2, the responsivity (R) and specific detectivity (D*) reached 20 mA/W and 107 cm Hz1/2 W 1 (Jones), and the external quantum efficiency (EQE) is 10%. Meanwhile, the prepared photodetector could operate at zero bias, i.e., self-powered operation, along with a photocurrent of about 1 nA under illumination with UV light intensity of 200 μW/cm2.