검단산(경기도 하남시)의 거미상

김주필(Joo Pil Kim),최한석(Han Seak Choi),이형민(Hyung Min Lee),이형진(Hyung Jeen Lee),이태후(Tae Hoo Lee),심주영(Ju Young Sim),조윤규(Yoon Kyu Jo),조원규(Won Kyu Jo),이준이(Joon Lee Lee) 한국거미연구소 2018 한국거미 Vol.34 No.2

2018년 10월 2일부터 3일까지 경기도 하남시 소재 검단산을 주야로 한국거미연구회 회원 13명(김주필, 최한석, 이형민, 이형진, 심주영, 이태후, 조윤규, 조원규, 이준이, 이현동, 윤혜원, 이광섭, 김형주)이 채집한 결과 22과 43속 56종이 채집되어 이에 보고 발표하는 바이다. From October 2 to 3 in 2018. Koran Arachnological Study members investigated the Spiders in the whole area Gyeonggi-do Hanam-si, and reported 22 Families, 43 Genus 56 Species of spiders. The collected spiders are placed (stored) in the Joo-Pil Spider museum.

신경망분석을 이용한 축구경기 승,패 예측모형 개발 -2006독일월드컵 대회를 중심으로-

김주학 ( Joo Hak Kim ),노갑택 ( Gap Taik Ro ),박종성 ( Jong Sung Park ),이원희 ( Won Hi Lee ) 한국스포츠정책과학원(구 한국스포츠개발원) 2007 체육과학연구 Vol.18 No.4

본 연구는 축구 경기 기록자료를 활용하여 축구경기의 승·패를 예측할 수 있는 모형을 개발하는 데 있다. 활용된 경기기록 자료는 2006년 독일 월드컵대회에 나타난 축구경기 전체 64경기의 FIFA TSG공식자료와 M대학교 경기분석 프로그램을 활용하여 산출된 경기기록 자료를 사용하였다. 연구기법으로는 최근에 예측연구에 많이 활용되고 있는 신경망 분석을 이용하여 예측율을 확인 하였으며, 예측율은 2개의 예측모형으로 분류하여 결과를 산출하였다. 구체적인 내용 및 방법은 다음과 같다. 2006년 독일월드컵에 나타난 기록 관련 요인 중 경기 내적요인을 중심으로 축구 경기의 승·패와 관련한 요인을 탐색하고 2006년 독일월드컵 전 경기에 대한 비디오녹화를 실시하였으며, 녹화된 비디오를 통하여 기록요인에 대하여 실시간 축구 경기분석 프로그램을 활용, 승·패와 관련한 기록요인을 점수화하였다. 점수화된 기록요인은 점유율, 성공률 관련 등 여러 가지 요인이 있었으나 실제 경기에서 예측을 할 수 있는 요인을 전문가와 회의를 통하여 선정하고 선정된 요인을 바탕으로 신경망 분석방법을 이용하여 축구경기 승·패 예측 모형을 개발하여 예측율을 확인하였다. 본 연구의 결과는 다음과 같다. 첫째, 전문가회의를 통하여 기록화 할 수 있는 기록자료 요인을 중심으로 2006년 독일월드컵 전체 경기에 대하여 축구 경기분석 프로그램을 통하여 점수화하여 실제 다양한 요인이 내재된 경기에 대하여서도 내용분류를 통하여 점수화할 수 있음을 확인하였다. 둘째, 점수화된 경기 내적요인을 중심으로 신경망분석 방법을 통하여 축구경기에 대하여서도 승·패를 예측할 수 있음을 확인하였으며 타 종목에 대하여서도 실현 가능함을 확인할 수 있었다. 셋째, 축구경기의 승패에 대하여 87.5%의 예측율을 보임으로써 축구경기의 요인에 대한 중요도를 평가할 수 있는 연구의 기초를 확립하였다고 판단된다. This study was on purpose to investigate the various factors of soccer games into inner factors and classify performance factors objectively and scientifically using a soccer game analysis program and develop a model which can predict the result of the soccer games using the Neural NetWork Analysis that stands on the game graded record. The game recorded data was collected from the FIFA TSG official record and the data which was gained using a game analysis program. It has searched the factors related the game results which were focused on the game inner factors of the related factors in 2006 World cup games and recorded the whole games of 2006 World cup games, graded the recording factors related the win/lose using the video and the real time game analysis program with numbers. The graded recording factors with numbers were several; ball possession, completion, and so on. However, through the meeting with Neural NetWork Analysis, professionals prediction related factors which can predict in the real games were selected. And a soccer game win/lose prediction model was developed using the selected factors and Neural NetWork Analysis. It also was compared with the preceding researches in documents record studies. Followings are main results of this study; First, It was confirmed that it is available to grade the real factors in the games which include various factors through the professionals` meetings which implemented the analysis the 2006 Worldcup games and it was focused on the recording factors. Second, the graded recording inner-factors make it possible to predict the soccer game results using the Neural NetWork Analysis method. It was verified that it can be effective in other sports also. Third, the rate of predicting the soccer games` results was 87.5%. It is possible to understand this study contributes on the basis of the importancy of soccer game factors.

화야산(경기도 가평군)의 거미상

김주필(Joo Pil Kim),최한석(Han Seak Choi),이형민(Hyung Min Lee),이형진(Hyung Jeen Lee),이태후(Tae Hoo Lee),심주영(Ju Young Sim),조윤규(Yoon Kyu Jo),조원규(Won Kyu Jo),이준이(Joon Lee Lee) 한국거미연구소 2018 한국거미 Vol.34 No.2

2018년 10월 13일부터 14일까지 경기도 가평군 소재 화야산을 주야로 한국거미연구회 회원 13명(김주필, 최한석, 이형민, 이형진, 심주영, 이태후, 조윤규, 조원규, 이준이, 이현동, 윤혜원, 이광섭, 김형주)이 채집한 결과 18과 41속 48종이 채집되어 이에 보고 발표하는 바이다. From October 13 to 14 in 2018. Koran Arachnological Study members investigated the Spiders in the whole area Gyeonggi-do Gapyeong-gun, and reported 18 Families 41 Genus 48 Species of spiders. The collected spiders are placed (stored) in the Joo-Pil Spider museum.

알레르기 환자에서 한방 이외의 대체 요법의 사용 현황에 대한 다기관 조사

이현정 ( Hyun Jung Lee ),이재현 ( Jae Hyun Lee ),이용원 ( Yong Won Lee ),김철우 ( Cheol Woo Kim ),동헌종 ( Hun Jong Dhong ),박해심 ( Hae Sim Park ),조영주 ( Young Joo Cho ),조진희 ( Jin Hee Cho ),조상헌 ( Sang Heon Cho ),편복양 ( 대한내과학회 2011 대한내과학회지 Vol.80 No.1

Background/Aims: The use of unproven complementary/alternative medicine (CAM) to treat allergies is popular in Korea. We conducted a multicenter survey of the current use of CAM other than herbal medication in Korean allergy patients. Methods: This study enrolled 510 adults with allergic diseases, including asthma, allergic rhinitis, atopic dermatitis, and chronic urticaria, from ten hospitals. They underwent a structured questionnaire interview and clinical assessment of the prevalence, motivation, costs, and subjective assessment of CAM. Results: Of the patients, 16.7% used at least one type of CAM to treat their allergic diseases. Common types of CAM were diet (48.6%), aroma therapy (23.8%), massage (14.3%), breathing exercises (7.6%), baths (5.7%), and mind control (5.7%). The therapeutic effects of CAM included improved in patients using mind control (50%), diet (50%), breathing exercises (75%), and baths (33.3%), but worse in patients using aroma therapy (28%), and massage (26.7%). About 36.4% of the patients used CAM more than four times per year, and the average cost per CAM user was 370,000 won/year. The main reason for trying CAM was `hope to improve my constitution`. Conclusions: CAM is used widely for treating allergic diseases in Korea. Detailed knowledge of CAM and patient education are important. Further studies of the clinical efficacy of CAM are needed. (Korean J Med 2011;80:68-77)

Genetic Characterization of Haemagglutinin 1 Domain of Influenza B Viruses Isolated in Korea during $1988{\sim}1999$

Shin, Gu-Choul,Na, Byoung-Kuk,Lee, Joo-Yeon,Ahn, Jung-Bae,Park, Jong-Won,Lee, Jin-Soo,Kim, Jee-Hee,Kim, Woo-Joo,Kang, Chun The Korean Society for Microbiology 2003 Journal of Bacteriology and Virology Vol.33 No.4

Although Korean influenza virus isolates have been genetically associated with the vaccine strains of the corresponding year, influenza B viruses have prevailed almost every year in Korea during the past decades. We have 'analyzed the genetic characteristics and evolutionary patterns of the haemagglutinin (HA) 1 domains of influenza B viruses isolated during 1988-1999 using direct RT-PCR and sequencing. Phylogenetic analysis of influenza B viruses isolated in Korea indicated that antigenically and genetically distinguishable strains of the lineage II and lineage III variants had been cocirculated. Variants prevailed in early 1990s are represented in 1996/97 and 1998/99 and some different variants have been co circulated geographically and prevailed concurrently in Korea. All HA1s of Korean isolates have amino acid substitutions mainly in the region between position 124 and 310, which was previously proposed an immunodominant region. Insertion-deletion patterns of the HA gene revealed that Korean influenza B viruses were evolved from Lee40 with different manner between lineage II and III viruses. Lineage III viruses were also divided into two groups as conserved group and inserted group, in relation to Lee40. But lineage II viruses had evolved with directional pattern. Antigenic index proposed that influenza B isolates prevailed since 1996/97 seasons might had emerged from the antigenic variants of a Seo1697-like virus and that new variants might appear from the lineage II viruses resulting in persistent prevalence in Korea.

Cytotoxic Effects on HL-60 Cells of Myosin Light Chain Kinase Inhibitor ML-7 Alone and in Combination with Flavonoids

Lee, Joong-Won,Kim, Yang-Jee,Choi, Young-Joo,Woo, Hae-Dong,Kim, Gye-Eun,Ha, Tae-Kyung,Lee, Young-Hyun,Chung, Hai-Won Korean Society of ToxicologyKorea Environmental Mu 2009 Toxicological Research Vol.26 No.4

Uncontrolled cell growth and increased cell proliferation are major features of cancer that are dependent on the stable structure and dynamics of the cytoskeleton. Since stable cytoskeleton structure and dynamics are partly regulated by myosin light chain kinase (MLCK), many current studies focused on MLCK inhibition as a chemotherapeutic target. As a potent and selective MLCK inhibitor, ML-7 [1-(5-iodonaphthalene-1-sulfonyl)-1 H-hexahydro-1,4-diazapine hydrochloride] is a promising candidate for an anticancer agent, which would induce apoptosis as well as prevents invasion and metastasis in certain types of cancer cells. This study assessed cytotoxic effects of ML-7 against HL-60 cells and therapeutic efficacy of ML-7 as a potential antileukemia agent. Trypan-blue exclusion assays showed dose- and time- dependent decreases in ML-7 treated HL-60 cells (p<0.05). Comet assays revealed a significant increase in DNA damage in HL-60 cells after treatment with $40{\mu}M$ ML-7 for 2h. Sub-G1 fractions, analyzed by flow cytometry increased in a dose-dependent manner, suggesting that ML-7 can induce apoptotic cell death in HL-60 cells. ML-7 was selectively cytotoxic towards HL-60 cells; not affecting normal human lymphocytes. That selective effect makes it a promising potential anti-leukemia agent. In addition, anticancer efficacy of ML-7 in combination with flavonoids (genistein or quercetin) or anticancer drugs (cisplatin or Ara-C) against HL-60 cells was assessed. Combination of ML-7 with flavonoids increased the anti-cancer effect of ML-7 to a greater extent than combination with the anticancer drugs. This implies that ML-7 in combination with flavonoids could increase the efficacy of anticancer treatment, while avoiding side effects cansed by conventional anticancer drug-containing combination chemotherapy.

Amelioration of Streptozotocin-Induced Diabetes by Agrocybe chaxingu Polysaccharide

Lee, Byung-Ryong,Lee, Yeom-Pyo,Kim, Dae-Won,Song, Ha-Yong,Yoo, Ki-Yeon,Won, Moo-Ho,Kang, Tae-Cheon,Lee, Kwang-Jae,Kim, Kyung-Hee,Joo, Jin-Ho,Ham, Hun-Ju,Hur, Jang-Hyun,Cho, Sung-Woo,Han, Kyu-Hyung,Lee Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.29 No.4

The aim of this study was to investigate the preventive effect of Agrocybe chaxingu polysaccharide on streptozocin (STZ)-induced pancreatic ${\beta}$-cells destruction. Agrocybe chaxingu polysaccharide markedly reduced nitric oxide (NO) production and iNOS expression levels in RINm5F cells in a dose-dependent manner. In addition, Agrocybe chaxingu polysaccharide significantly inhibited iNOS expression and blood glucose levels in STZ-induced diabetic mice. Moreover, immunohistochemical analysis revealed that it enhanced pancreatic ${\beta}$-cells resistance to destruction by STZ. These results suggest that Agrocybe chaxingu polysaccharide may have value as a therapeutic agent against diabetes mellitus.

Ferritin, an Iron Storage Protein, Associates with Kinesin 1 through the Cargo-binding Region of Kinesin Heavy Chains (KHCs)

Won Hee Jang(장원희),Young Joo Jeong(정영주),Won Hee Lee(이원희),Mooseong Kim(김무성),Sang-Jin Kim(김상진),Sang-Hwa Urm(엄상화),Il Soo Moon(문일수),Dae-Hyun Seog(석대현) 한국생명과학회 2016 생명과학회지 Vol.26 No.6

세포내소기관과 단백질 복합체의 운반은 kinesin superfamily proteins (KIFs)에 의해 매개된다. 처음으로 밝혀진 kinesin인 kinesin 1은 motor단백질로서 세포 내에서 미세소관을 따라 이동하며, 다양한 세포내 소기관이나 단백질복합체를 운반한다. Kinesin 1은 장쇄(KHC, 또한 KIF5s로도 통칭) 2분자와 단쇄(KLCs) 2분자로 구성된 4합체(tetramer) 구조를 가진다. KIF5s의 운반체 결합영역을 포함하는 말단영역은 다수의 운반체와 결합하지만, 결합운반체에 관하여 아직 충분히 밝혀지지 않았다. 본 연구에서 KIF5A의 결합 단백질을 동정하기 위하여 효모 two-hybrid screening을 수행하였고 철 저장 및 해독 기능을 하는 단백질인 ferritin heavy chain (Frt-h)을 찾아내었다. Frt-h은 KIF5A의 아미노산 800번과 940번 사이의 부위와 결합하며, 다른 KIF5s와도 결합함을 효모 two-hybrid assay로 확인하였다. 또한 Frt-h의 coiled-coil 도메인이 KIF5A와의 결합에 필수영역임을 밝혔다. 한편, ferritin light chain (Frt-l) 또한 KIF5s와 결합함을 효모 two-hybrid assay로 확인하였다. 이러한 단백질간의 결합을 glutathione S-transferase (GST) pull-down assay를 통하여 검증하였다. 추가적으로 생쥐의 뇌 파쇄액을 항 KHC 항체로 면역침강을 행한 결과, KLC1뿐만 아니라 Frt-h와 Frt-l도 같이 침강하였다. 이러한 결과들은 세포 내에서 kinesin 1이 ferritin 복합체를 운반함을 시사한다. The intracellular transport of organelles and protein complexes is mediated by kinesin superfamily proteins (KIFs). The first kinesin, kinesin 1, was identified as a molecular motor protein that moves various organelles and protein complexes along the microtubule rails in cells. Kinesin 1 is a tetramer of two heavy chains (KHCs, also called KIF5s) and two kinesin light chains (KLCs). KIF5s interact with many different proteins through their tail region, but their binding proteins have not yet been fully identified. To identify the interaction proteins for KIF5A, we performed yeast two-hybrid screening and found a specific interaction with ferritin heavy chain (Frt-h), which has a role in iron storage and detoxification. Frt-h bound to the amino acid residues between 800 and 940 of KIF5A and to other KIF5s in the yeast two-hybrid assay. The coiled-coil domain of Frt-h is essential for interaction with KIF5A. In addition, ferritin light chain (Frt-l) interacted with KIF5s in the yeast two-hybrid assay. In addition, these proteins showed specific interactions in the glutathione S-transferase (GST) pull-down assay. An antibody to KHC specifically co-immunoprecipitated Frt-h and Frt-l from mouse brain extracts. These results suggest the kinesin 1 motor protein may transport the ferritin complex in cells.

Rituximab treatment for autoimmune limbic encephalitis in an institutional cohort

Lee, Woo-Jin,Lee, Soon-Tae,Byun, Jung-Ick,Sunwoo, Jun-Sang,Kim, Tae-Joon,Lim, Jung-Ah,Moon, Jangsup,Lee, Han Sang,Shin, Yong-Won,Lee, Keon-Joo,Kim, Soyun,Jung, Keun-Hwa,Jung, Ki-Young,Chu, Kon,Lee, Sa Ovid Technologies (Wolters Kluwer) - American Acad 2016 Neurology Vol.86 No.18

<P>Objective: To determine efficacy and safety of rituximab treatment as a second-line immunotherapy treatment for autoimmune limbic encephalitis (ALE) and to determine factors associated with functional improvement and favorable outcome following rituximab treatment. Methods: We recruited 80 patients with ALE who were treated with rituximab as a second-line immunotherapy from the Korea Autoimmune Synaptic and Paraneoplastic Encephalitis Registry and reviewed 81 patients without rituximab as a control. We grouped patients according to the detection or type of antibodies; in addition, we evaluated clinical, laboratory, first-line immunotherapy, and rituximab treatment profiles and defined main outcomes as improvements on the modified Rankin Scale (mRS) score and a favorable mRS score (0-2) at the last follow-up. Results: Functional improvement occurred more frequently in the rituximab group compared to the control group. In the rituximab group, 30 (37.5%) patients had synaptic autoantibodies, 15 (18.8%) in the paraneoplastic autoantibodies, and 35 (43.8%) were antibody-negative. The effect of rituximab was the same regardless of autoantibody status. Additional monthly rituximab therapy and partial response to first-line immunotherapies were associated with mRS score improvements, as well as favorable mRS scores. mRS scores of 4-6 as the worst neurologic status predicted an unfavorable mRS score. There were no reported serious infusion-related or infectious adverse effects of rituximab. Conclusions: Rituximab is effective and safe as a second-line immunotherapy for ALE, regardless of autoantibody status. Additional monthly rituximab therapy might potentiate the efficacy of rituximab. Classification of evidence: This study provides Class IV evidence that rituximab improves mRS scores for patients with autoimmune limbic encephalitis who fail first-line therapy.</P>

Development and Validation of a Novel Difficulty Scoring System for Liver Re-resection for Hepatocellular Carcinoma with Previous Hepatectomy

Joo Hyun Bang,Jai Young Cho,Ho-Seong Han,Yoo-Seok Yoon,Hae won Lee,Boram Lee,Yeshong Park,Jinju Kim,MeeYoung Kang,Eunhye Lee 대한외과학회 2023 대한외과학회 학술대회 초록집 Vol.2023 No.5