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한우에 감염된 Theileria sergenti merozoite의 순수분리와 genomic DNA probe에 관한 연구
채준석,이주묵,권오덕,채건상 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.17 No.-
To make the genomic DNA probe of Theileria sergenti, the merozoites were purified from bovine crythrocytes. The infected erythrocytes were lysed by Aeromonas hydrophila(Ah-1) hemolysin, and the parasites were isolated by ultracentrifugation on a Percoll discontinuous density gradient. For construction of a T sergenti genomic DNA library, T sergenti DNA was digested with PstI and the fragments were ligated into the PstI site of pUC19 before transformation of Escherichia coli JM83. Out of thousands of transformants obtained by transformation of E coli JM83 with the genomic library, three plasmids were chosen. The sizes of the inserted DNAs were 2.9kb(2.4kb and 0.5kb) in pKTS1, 4.3kb in pKTS2 and 1.5kb in pKTS3, respectively. The DNA fragments used as probe KTS1(2.4kb), KTS2(4.3kb) and KTS3(1.5kb) were labeled digoxigenin-11-dUTP for the Southern hybridization. In Southern hybridization, all of the probes(KTS1, KTS2 and KTS3) reacted specifically to T sergenti DNA, but not to bovine leucocyte DNA. In order to find out the sensitivities of the digoxigenin-11-dUTP-labeled KTS1 and KTS3 as the probes, purified merozoite DNA and bovine DNA(control) were checked by dot blot hybridization with the probes. Both of the probes, KTS1 and KTS3, detected as minimum amount of 975pg of the T sergenti DNA, but not bovine DNA even to 500ng.
소정온,허지연,심혜진,김종원,나덕렬,이필휴,정선주,박문호,주인수,송미숙,김영호,묵인희 대한치매학회 2004 Dementia and Neurocognitive Disorders Vol.3 No.1
Background:Alzheimer's disease (Ad) is a neurodegenerative disorder that is rapidly increasing with the aging society, requiring a need for early diagnosis and prevention. However, diagnosis on AD has only been possible through limited methods such as neuropsychological examination or MRI. AD is characterized by deposition of senile plaques and neurofibrillary tangles in the brain. Aβ peptide in senile plaques seems to play a central role in the neuropathology of AD. Several biochemical markers for AD are available, including reduced Aβ protein, a change in ratio between Aβ40 and 42 and increased level of tau protein in the cerebrospinal fluid (CSF). Methods:This study analyzes anti-Aβ antibody from serums of AD patients using the ELISA. The levels of anti-Aβ antibody from patients with idiopathic Parkinson's disease or stroke and from normal control were compared to that of AD patients. Results:Our results showed a significantly lower anti-Aβ antibody level in AD compared to those with other neurological diseases or control. Conclusions:These data showed that the anti-Aβ antibody level in the serum may be used to diagnose the presence of AD.
Lee, Joo-Young,Lee, Eun-Song,Kang, Si-Mook,Kim, Baek-Il ELSEVIER SCIENCE B.V., AMSTERDAM 2019 PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY Vol.25 No.-
<P><B>Abstract</B></P> <P><B>Objectives</B></P> <P>This study aimed to determine the efficacy of a combination of photocatalysts—hydrogen peroxide at a low concentration (3.5%) and titanium dioxide (TiO<SUB>2</SUB>)—activated at a wavelength of 405 nm using quantitative light-induced fluorescence (QLF) technology, and to quantify their tooth-bleaching efficacy using fluorescence images obtained from QLF technology.</P> <P><B>Materials and methods</B></P> <P>Forty bovine incisors were extrinsically stained according to Stookey’s method, and were randomly divided into four groups (<I>n</I> = 10 per group). Two bleaching solutions were prepared by mixing 3.5% H<SUB>2</SUB>O<SUB>2</SUB> with 0.05% of anatase and rutile TiO<SUB>2</SUB> powders. These solutions were applied to the stained teeth using a microbrush and then irradiated for 15 min at either 306 or 405 nm to activate the bleaching agent. The color difference (Δ<I>E<SUP>*</SUP> </I>) was assessed before and after every 5 min of treatment. The Δ<I>E</I> <SUP>*</SUP> and the changes in the fluorescence loss (ΔΔ<I>F</I>) were obtained from white-light and fluorescence images, respectively.</P> <P><B>Results</B></P> <P>All of the low-H<SUB>2</SUB>O<SUB>2</SUB>/TiO<SUB>2</SUB> treatments caused significant tooth-bleaching efficacy after irradiation at 306 and 405 nm (<I>p</I> < 0.05). The results did not differ significantly between the two wavelengths (<I>p</I> > 0.05), but the bleaching efficacy was greater with anatase TiO<SUB>2</SUB> at 306 nm and rutile TiO<SUB>2</SUB> at 405 nm. Analysis of the fluorescence images revealed that the Δ<I>F</I> values increased significantly in all groups with the treatment time (<I>p</I> < 0.05). There was a statistically significant correlation between Δ<I>E<SUP>*</SUP> </I> and the change in ΔΔ<I>F</I> (<I>r</I> = 0.822, <I>p</I> < 0.001).</P> <P><B>Conclusions</B></P> <P>Combining low-H<SUB>2</SUB>O<SUB>2</SUB>/TiO<SUB>2</SUB> with QLF technology at 405 nm has an efficacy of tooth-bleaching as a less harmful and biofriendly method, while the fluorescence images obtained by QLF technology could be used to assess tooth-bleaching.</P> <P><B>Highlights</B></P> <P> <UL> <LI> This study focused on the new concept of theragnosis in field of tooth bleaching. </LI> <LI> Tooth bleaching efficacy was improved by combining low H<SUB>2</SUB>O<SUB>2</SUB>/TiO<SUB>2</SUB> under 405 nm. </LI> <LI> Fluorescence images could be used to assess tooth bleaching. </LI> <LI> QLF technology showed dual increasing and assessing efficacy of tooth bleaching. </LI> <LI> In tooth bleaching field, QLF technology has possibility of realizing theragnosis. </LI> </UL> </P>
Effect of quercetin on apoptosis of PANC-1 cells
Joo Hyun Lee,Han-Beom Lee,Gum O Jung,Jung Taek Oh,Dong Eun Park,Kwon Mook Chae 대한외과학회 2013 Annals of Surgical Treatment and Research(ASRT) Vol.85 No.6
Purpose: To investigate the chemotherapeutic effect of quercetin against cancer cells, signaling pathway of apoptosis was explored in human pancreatic cells. Methods: Various anticancer drugs including adriamycin, cisplatin, 5-fluorouracil (5-FU) and gemcitabine were used. Cell viability was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphe-nyltetra zolium bromide assay. Apoptosis was determined by 4’-6-diamidino-2-phenylindole nuclei staining and flow cytometry in PANC-1 cells treated with 50 μg/mL quercetin for 24 hours. Expression of endoplas mic reticulum (ER) stress mediators including, Grp78/Bip, p-PERK, PERK, ATF4, ATF6 and GADD153/CHOP proteins were measured by Western blot analysis. Mitochondrial membrane potential was measured by fluorescence staining with JC-1, rhodamine 123. Quercetin induced the apoptosis of PANC-1, which was characterized as nucleic acid and genomic DNA fragmentation, chromatin condensation, and sub-G0/G1 fraction of cell cycle increase. But not adriamycin, cisplatin, gemcitabine, and 5-FU. PANC-1 cells were markedly sensitive to quercetin. Results: Treatment with quercetin resulted in the increased accumulation of intracellular Ca<SUP>2+</SUP> ion. Treatment with quercetin also increased the expression of Grp78/Bip and GADD153/CHOP protein and induced mitochondrial dysfunction. Quercetin exerted cytotoxicity against human pancreatic cancer cells via ER stressmediated apoptotic signaling including reactive oxygen species production and mitochondrial dysfunction. Conclusion: These data suggest that quercetin may be an important modulator of chemosensitivity of cancer cells against anticancer chemotherapeutic agents.
Lee, Sang-Mook,Lee, Young-Joo,Lee, Jae-Wook The Polymer Society of Korea 2007 Macromolecular Research Vol.15 No.1
This study investigated the effect of ultrasound irradiation on the blend of poly(lactic acid) (PLA) and poly(butylene adipate-co-terephthalate) (PBAT). The blends of PLA/PBAT(50/50) (PBAT50) were prepared in a melt mixer with an ultrasonic device attached. Thermal, rheological, and mechanical properties, morphology, and biodegradability of the sonicated blends were analysed. The viscosity of the sonicated blends was increased by the ultrasound irradiation owing to the strong interaction. The morphology of the sonicated blends was significantly dependent on the duration o the ultrasound irradiation. For PBAT50, the phase size reduction was maximized when the blends were ultrasonically irradiated for 30 sec. At longer duration of ultrasound irradiation, the PBAT phase underwent flocculation. Measurement of the tensile properties showed an increased breakage tensile stress and an enhanced Young's modulus when the blends were properly irradiated. This improvement was ascribed to better adhesion between the PLA matrix and the PBAT domain and to better dispersion of the PBAT phase. However, the tensile properties were maximized after excessive energy irradiation, which was ascribed to an emulsifying effect leading to coalescence of the PBAT phase. Impact strength was increased to reach a peak with the ultrasound irradiation, and was higher than the untreated sample for all sonicated samples due to the difference of failure mechanism between the tensile test and the impact test.
Lee, Seung-Mook,Rhee, Bum-Ku,Lee, Sang-Ho,Lee, Chul-Joo,Park, Ki-Hong Korean Society of Photoscience 2003 Journal of Photosciences Vol.10 No.2
Two coupled molecules were successfully synthesized by condensation of amine-donor-substituted barbituric acid derivativies as nonlinear optical chromophores. A flexible spacer of the alkyl chain with different lengths of carbon chains (5 and 6 carbons) was introduced between two chromophores, which prevented crystallization and aggregation of molecules. Two coupled molecules (B-Cn-B, n=5, 6) had glass-transition temperatures on a second heating around 81 and 76$^{\circ}C$ without melting points, respectively. To explore the linear optical properties, thin-films were prepared and examined by a photometry method using Nd:YVO$_4$ CW laser. Also, microscopic and macroscopic nonlinear optical properties were measured by Hyper-Rayleigh Scattering (HRS) and the Maker Fringes method using Nd:YAG ps pulse laser, respectively. In spite of the moderate hyperpolarizabilities of coupled molecules, the second order NLO coefficient (d$\_$33/) was larger than the conventional Disperse Red 1 doped PMMA polymeric system.