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OsAMT1.3 expression alters rice ammonium uptake kinetics and root morphology
Leandro Martins Ferreira,Vinicius Miranda de Souza,Orlando Carlos Huertas Tavares,Everaldo Zonta,Claudete Santa-Catarina,Sonia Regina de Souza,Manlio Silvestre Fernandes,Leandro Azevedo Santos 한국식물생명공학회 2015 Plant biotechnology reports Vol.9 No.4
High-affinity ammonium transporters (AMT1) are responsible for ammonium (NH4 ?) acquisition and/or perception in the micromolar range, and their expressions can be differentially regulated by nitrogen (N) availability. The present study characterised the functions of the rice (Oryza sativa) OsAMT1.3 transporter to understand its contribution to NH4+ acquisition and plant adaptation to environments with low N availability. Transgenic rice plants were obtained to study the activity of the OsAMT1.3 promoter (POsAMT1.3:GFP:GUS) and the overexpression of the OsAMT1.3 gene (UBIL:OsAMT1.3:3xHA) in plants. The OsAMT1.3 promoter activity was induced strongly in the absence of N and occurred primarily in the zones of lateral root emission and root tips. Anatomical sections of the segment of root tips and the middle third showed a differential pattern of OsAMT1.3 activity. Analysis of the OsAMT1.1–1.3 transporter expression profiles indicated that overexpression of OsAMT1.3 positively affected OsAMT1.2 expression. When subjected to a low N supply, plants overexpressing OsAMT1.3 showed lower KM and Cmin values. Additionally, these lines showed longer roots with a higher area, volume, and number of tips. The data suggested that OsAMT1.3 is involved in the ability of rice plants to adapt to low NH4+ supplies.
Leandro C. Luiz,Francisco J.O. Ferreira,Verginia R. Crispim 한국원자력학회 2017 Nuclear Engineering and Technology Vol.49 No.4
The presence of crust on the inner walls of metallic ducts impairs transportation becausecrust completely or partially hinders the passage of fluid to the processing unit and causesdamage to equipment connected to the production line. Its localization is crucial. With thedevelopment of the electronic imaging system installed at the Argonauta/Nuclear EngineeringInstitute (IEN)/National Nuclear Energy Commission (CNEN) reactor, it becamepossible to visualize crust in the interior of metallic piping of small diameter using realtimeneutron radiography images obtained with a low neutron flux. The obtained imagesshowed the resistance offered by crust on the passage of water inside the pipe. Nodiscrepancy of the flow profile at the bottom of the pipe, before the crust region, wasregistered. However, after the passage of liquid through the pipe, images of the disturbancesof the flow were clear and discrepancies in the flow profile were steep. This showsthat this technique added the assembled apparatus was efficient for the visualization ofthe crust and of the two-phase flows.
Cementitious Porous Material Applied to Precision Aerostatics Bearings
Leandro Jos da Silva,Tulio Hallak Panzera,Luciano Machado Gomes Viera,Christopher Rhys Bowen,Jaime Gilberto Duduch,Juan Carlos Campos Rubio 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.19 No.2
The use of porous materials as restrictor in aerostatic bearings provides many advantages over conventional restrictors, such as small variation of temperature, high damping, high operational speeds, limited wear and capacity to support radial, axial, and combined loads, being considered important features for precision machines and instruments. This work evaluates the load carrying capacity for different air gaps and pressures of thrust porous bearing made with cementitious composites. The cementitious composites consisted of Portland cement and monomodal silica particles (44 μm) were fabricated via uniaxial cold-pressing (10 MPa). The load capacity was determined for different air pressures, such as 3, 4, 5 and 6 bar. The air gap was measured using pneumatic transducers. A pneumatic instability was observed when the air pressure level increased from 3 to 6 bar. A similar loading capacity, for bearing gaps between 7 and 30 μm, was achieved in comparison to hot-pressed porous alumina found in the literature. In addition, the cementitious porous bearing provided a superior loading capacity for gaps higher than 10 μm when compared to graphite porous bearing found in the literature. The results revealed the cementitious composites are promising materials for porous restrictor in aerostatic thrust bearings.
( Leandro Val Sayson ),( Mikyung Kim ),( Se Jin Jeon ),( Raly James Perez Custodio ),( Hyun Jun Lee ),( Darlene Mae Ortiz ),( Jae Hoon Cheong ),( Hee Jin Kim ) 한국응용약물학회 2022 Biomolecules & Therapeutics(구 응용약물학회지) Vol.30 No.3
Previous reports have demonstrated that genetic mechanisms greatly mediate responses to drugs of abuse, including methamphetamine (METH). The circadian gene Period 2 (Per2) has been previously associated with differential responses towards METH in mice. While the behavioral consequences of eliminating Per2 have been illustrated previously, Per2 overexpression has not yet been comprehensively described; although, Per2-overexpressing (Per2 OE) mice previously showed reduced sensitivity towards METH-induced addiction-like behaviors. To further elucidate this distinct behavior of Per2 OE mice to METH, we identified possible candidate biomarkers by determining striatal differentially expressed genes (DEGs) in both drug-naïve and METH-treated Per2 OE mice relative to wild-type (WT), through RNA sequencing. Of the several DEGs in drug naïve Per2 OE mice, we identified six genes that were altered after repeated METH treatment in WT mice, but not in Per2 OE mice. These results, validated by quantitative real-time polymerase chain reaction, could suggest that the identified DEGs might underlie the previously reported weaker METH-induced responses of Per2 OE mice compared to WT. Gene network analysis also revealed that Asic3, Hba-a1, and Rnf17 are possibly associated with Per2 through physical interactions and predicted correlations, and might potentially participate in addiction. Inhibiting the functional protein of Asic3 prior to METH administration resulted in the partial reduction of METH-induced conditioned place preference in WT mice, supporting a possible involvement of Asic3 in METH-induced reward. Although encouraging further investigations, our findings suggest that these DEGs, including Asic3, may play significant roles in the lower sensitivity of Per2 OE mice to METH.
Leandro Freire dos Santos,Ana Lucia Zanatta,Vanete Thomaz Soccol,Maria Fernanda Torres,Sandro José Ribeiro Bonatto,Rosália Rubel,Carlos Ricardo Soccol 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.1
The ability of Pleurotus ostreatus biomass,cultived by submerged fermentation, to produce beneficial effect on lipid profile and macrophages activity during a high-fat diet (HFD) for a long-term intake was investigated. Blood samples were collected through cardiac puncture to measure the plasma cholesterol, triglycerides, low-density protein (LDL), high-density protein (HDL), aspartate aminotransferase (AST) activity, urea– blood urea nitrogen (BUN)/creatinine ratio of rats fed on an HFD for 4 months. Dosage of lipid hydroperoxides was carried out on methanolic extract of liver tissue. Peritoneal macrophages activity was evaluated in relation to the superoxide anion,hydrogen peroxide and nitric oxide production, phagocytosis and lysosomal volume. The administration of P. ostreatus significantly altered the lipid profile and oxidative stress as related to the LDL and triglycerides decrease and inhibitory effects on superoxide anion and hydrogen peroxide production. All findings of this study lead us to suggest that the P. ostreatus maybe a beneficial agent in the hyperlipidemia and atherosclerosis treatments.
Leandro dos Santos,Leticia de Oliveira Maciel,Erika Kristina Incerpi Gracia,Evelise Aline Soares,Danielle Aparecida Quintino Si,Valerio Garrone Barauna,Heder Frank Gianotto Estrela,Carollayne Mendonca 한국식품영양과학회 2021 Preventive Nutrition and Food Science Vol.26 No.1
Cardiovascular diseases are the leading cause of mortality and morbidity worldwide. In this study we compared the effects of oral treatment with red pepper ethereal extracts or simvastatin on dyslipidemia, left ventricle remodeling, and atherosclerotic lesions of low-density lipoprotein (LDL) receptor knockout mice (LDLr<SUP>−/−</SUP>) fed a hyperlipidic diet. Forty 3-month-old male mice were distributed into four groups: control (C; animals fed a standard diet), HL (animals fed a hyperlipidic diet), and HL+P or HL+S (animals fed a hyperlipidic diet plus red pepper ethereal extracts or simvastatin, respectively). After 60 days, treatment with both red pepper ethereal extracts and simvastatin prevented dyslipidemia, atherosclerotic lesion progression, and left ventricle hypertrophy. Our results suggest a cardioprotective effect of red pepper ethereal extracts in LDLr<SUP>−/−</SUP> mice, which is comparable to the well-known effects of simvastatin.
Leandro L. de Souza,Fernanda L.B. de Araujo,Fernanda A.M. da Silva,Thaís S. Mucciaroni,João E. de Araujo 사단법인약침학회 2016 Journal of Acupuncture & Meridian Studies Vol.9 No.5
We previously showed that a yin meridian of the upper limb decreased electromyographic activity (root mean square) and muscle strength ipsilateral and contralateral to the side of stimulation. Here, we tested the upper trapezius (UT) muscle response after stimulation of a yang meridian of the upper limb, the small intestine (SI). Thirty-eight healthy volunteers were randomized into the following groups: UT muscle (SI14), distant of the UT muscle (SI8), without stimulation (CG), and sham (R3). An acupuncturist certificated by the Brazilian Society of Physical Therapists and Acupuncturists performed the needle insertion. Each volunteer received only one stimulation to the right upper limb. The evaluation occurred before, 5 minutes after, and 20 minutes after needle withdrawal. The root mean square activity increased on the right side in the UT muscle for the SI8 and SI14 groups (F3,37 = 4.67; p < 0.025) at the 20-minute evaluation. The most vigorous response occurred on the contralateral side because the effects were maintained for 5 minutes after withdrawal (F3,37 = 4.52; p < 0.025). Both groups showed an increase in the UT muscle strength at the 20-minute evaluation (F3,37 = 3.41;p < 0.025). The CG and R3 groups did not show any changes. Our data indicate that SI a yang meridian increases the UT muscle response.
OsDof25 expression alters carbon and nitrogen metabolism in Arabidopsis under high N-supply
Leandro Azevedo Santos,Sonia Regina de Souza,Manlio Silvestre Fernandes 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.4
To verify the effect of the transcription factor OsDof25 on the nitrogen metabolism in plants, it was cloned for expression in Arabidopsis thaliana under the control of the 35S promoter. Lineages with different expression levels of this gene were obtained, and when grown in a half-strength MS medium (10 mM of NH4? and 20 mM of NO3-) showed phenotypes with visible chloroses and growth disorders. It was observed that OsDof25increased the levels of expression of high and low affinity ammonium transporters (AtAMT1.1 and AtAMT2.1,respectively) and repressed the high affinity nitrate transporter (AtNRT2.1). We also verified an increase in total amino-N content and expressions of the pyruvate kinase (PK1 and PK2), phosphoenolpyruvate carboxylase (PEPC1and PEPC2) and NADP-dependent and NAD-dependent isocitrate dehydrogenase. In addition, an increase in expression levels and activity of glutamate dehydrogenase was also observed. The simultaneous increase in the expression of AMTs and enzymes of carbon metabolism may explain the high levels of amino-N found in the transgenic plants. The data found in this work suggest that OsDof25 expression simultaneously affects NH4? uptake and organic acid metabolism in plants.
Leandro Fernandes Andrade,Gleika Larisse Oliveira Dorasio de Souza,Silvia Nietsche,Adelica Aparecida Xavier,Marcia Regina Costa,Acleide Maria Santos Cardoso,Marlon Cristian Toledo Pereira,Débora Franc 한국미생물학회 2014 The journal of microbiology Vol.52 No.1
A total of 40 endophytic bacterial isolates obtained from banana tree roots were characterized for their biotechnological potential for promoting banana tree growth. All isolates had at least one positive feature. Twenty isolates were likely diazotrophs and formed pellicles in nitrogen-free culture medium, and 67% of these isolates belonged to the genus Bacillus sp. The isolates EB-04, EB-169, EB-64, and EB-144had N fixation abilities as measured by the Kjeldahl method and by an acetylene reduction activity assay. Among the 40isolates, 37.5% were capable of solubilizing inorganic phosphate and the isolates EB-47 and EB-64 showed the highest solubilization capacity. The isolate EB-53 (Lysinibacillus sp.)had a high solubilization index, whereas 73% of the isolates had low solubilization indices. The synthesis of indole-3-acetic acid (IAA) in the presence of L-tryptophan was detected in 40% of the isolates. The isolate EB-40 (Bacillus sp.)produced the highest amount of IAA (47.88 μg/ml) in medium supplemented with L-tryptophan and was able to synthesize IAA in the absence of L-tryptophan. The isolates EB-126 (Bacillus subtilis) and EB-47 (Bacillus sp.) were able to simultaneously fix nitrogen, solubilize phosphate and produce IAA in vitro. The results of this study demonstrated that the isolates analyzed here had diverse abilities and all have the potential to be used as growth-promoting microbial inoculants for banana trees.