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- 저자 : Sonia Regina de Souza (검색결과 2 건)
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OsDof25 expression alters carbon and nitrogen metabolism in Arabidopsis under high N-supply
Leandro Azevedo Santos,Sonia Regina de Souza,Manlio Silvestre Fernandes 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.4
To verify the effect of the transcription factor OsDof25 on the nitrogen metabolism in plants, it was cloned for expression in Arabidopsis thaliana under the control of the 35S promoter. Lineages with different expression levels of this gene were obtained, and when grown in a half-strength MS medium (10 mM of NH4? and 20 mM of NO3-) showed phenotypes with visible chloroses and growth disorders. It was observed that OsDof25increased the levels of expression of high and low affinity ammonium transporters (AtAMT1.1 and AtAMT2.1,respectively) and repressed the high affinity nitrate transporter (AtNRT2.1). We also verified an increase in total amino-N content and expressions of the pyruvate kinase (PK1 and PK2), phosphoenolpyruvate carboxylase (PEPC1and PEPC2) and NADP-dependent and NAD-dependent isocitrate dehydrogenase. In addition, an increase in expression levels and activity of glutamate dehydrogenase was also observed. The simultaneous increase in the expression of AMTs and enzymes of carbon metabolism may explain the high levels of amino-N found in the transgenic plants. The data found in this work suggest that OsDof25 expression simultaneously affects NH4? uptake and organic acid metabolism in plants.
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OsAMT1.3 expression alters rice ammonium uptake kinetics and root morphology
Leandro Martins Ferreira,Vinicius Miranda de Souza,Orlando Carlos Huertas Tavares,Everaldo Zonta,Claudete Santa-Catarina,Sonia Regina de Souza,Manlio Silvestre Fernandes,Leandro Azevedo Santos 한국식물생명공학회 2015 Plant biotechnology reports Vol.9 No.4
High-affinity ammonium transporters (AMT1) are responsible for ammonium (NH4 ?) acquisition and/or perception in the micromolar range, and their expressions can be differentially regulated by nitrogen (N) availability. The present study characterised the functions of the rice (Oryza sativa) OsAMT1.3 transporter to understand its contribution to NH4+ acquisition and plant adaptation to environments with low N availability. Transgenic rice plants were obtained to study the activity of the OsAMT1.3 promoter (POsAMT1.3:GFP:GUS) and the overexpression of the OsAMT1.3 gene (UBIL:OsAMT1.3:3xHA) in plants. The OsAMT1.3 promoter activity was induced strongly in the absence of N and occurred primarily in the zones of lateral root emission and root tips. Anatomical sections of the segment of root tips and the middle third showed a differential pattern of OsAMT1.3 activity. Analysis of the OsAMT1.1–1.3 transporter expression profiles indicated that overexpression of OsAMT1.3 positively affected OsAMT1.2 expression. When subjected to a low N supply, plants overexpressing OsAMT1.3 showed lower KM and Cmin values. Additionally, these lines showed longer roots with a higher area, volume, and number of tips. The data suggested that OsAMT1.3 is involved in the ability of rice plants to adapt to low NH4+ supplies.
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