Ultrasound and clinical findings from metacarpophalangeal joint assessments in show jumping horses in training

Ana Lúcia M. Yamada,Marcelo Pinheiro,Marília F. Marsiglia,Stefano Carlo F. Hagen,Raquel Yvonne A. Baccarin,Luís Cláudio L. C. da Silva 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.3

The aims of this study were to determine the morphological patterns, physical examination characteristics and ultrasound findings of show jumping horses in training and to establish a score-based examination model for physical and ultrasound foBackground: Physical exercise is known to cause significant joint changes. Thus, monitoring joint behavior of athletic horses is essential in early disorders recognition, allowing the proper management. Objectives: The aims of this study were to determine the morphological patterns, physical examination characteristics and ultrasound findings of show jumping horses in training and to establish a score-based examination model for physical and ultrasound follow-ups of metacarpophalangeal joint changes in these animals. Methods: A total of 52 metacarpophalangeal joints from 26 horses who were initially in the taming stage were evaluated, and the horses’ athletic progression was monitored. The horses were evaluated by a physical examination and by B-mode and Doppler-mode ultrasound examinations, starting at time zero (T0), which occurred concomitantly with the beginning of training, and every 3 months thereafter for a follow-up period of 18 months. Results: The standardized examination model revealed an increase in the maximum joint flexion angles and higher scores on the physical and ultrasound examinations after scoring was performed by predefined assessment tools, especially between 3 and 6 months of evaluation, which was immediately after the horses started more intense training. The lameness score and the ultrasound examination score were slightly higher at the end of the study. Conclusions: The observed results were probably caused by the implementation of a traininBackground: Physical exercise is known to cause significant joint changes. Thus, monitoring joint behavior of athletic horses is essential in early disorders recognition, allowing the proper management. Objectives: The aims of this study were to determine the morphological patterns, physical examination characteristics and ultrasound findings of show jumping horses in training and to establish a score-based examination model for physical and ultrasound follow-ups of metacarpophalangeal joint changes in these animals. Methods: A total of 52 metacarpophalangeal joints from 26 horses who were initially in the taming stage were evaluated, and the horses’ athletic progression was monitored. The horses were evaluated by a physical examination and by B-mode and Doppler-mode ultrasound examinations, starting at time zero (T0), which occurred concomitantly with the beginning of training, and every 3 months thereafter for a follow-up period of 18 months. Results: The standardized examination model revealed an increase in the maximum joint flexion angles and higher scores on the physical and ultrasound examinations after scoring was performed by predefined assessment tools, especially between 3 and 6 months of evaluation, which was immediately after the horses started more intense training. The lameness score and the ultrasound examination score were slightly higher at the end of the study. Conclusions: The observed results were probably caused by the implementation of a training regimen and joint adaptation to physical conditioning. The joints most likely undergo a pre-osteoarthritic period due to work overload, which can manifest in a consistent or adaptive manner, as observed during this study. Thus, continuous monitoring of young athlete horses by physical and ultrasound examinations that can be scored is essential. g regimen and joint adaptation to physical conditioning. The joints most likely undergo a pre-osteoarthritic period due to work overload, which can manifest in a consistent or adaptive manner, as observed during this study. Thus, continuous monitoring of young athlete horses by physical and ultrasound examinations that can be scored is essential.llow-ups of metacarpophalangeal joint changes in these animals....

Inhibition of tyrosinase by fumaric acid: Integration of inhibition kinetics with computational docking simulations

Gou, L.,Lee, J.,Yang, J.M.,Park, Y.D.,Zhou, H.M.,Zhan, Y.,Lu, Z.R. Elsevier 2017 International Journal of Biological Macromolecules Vol.105 No.3

Fumaric acid (FA), which is naturally found in organisms, is a well known intermediate of the citric acid cycle. We evaluated the effects of FA on tyrosinase activity and structure via enzyme kinetics and computational simulations. FA was found to be a reversible inhibitor of tyrosinase and its induced mechanism was the parabolic non-competitive inhibition type with IC<SUB>50</SUB>=13.7+/-0.25mM and K<SUB>islope</SUB>=12.64+/-0.75mM. We newly established the equation for the dissociation constant (K<SUB>islope</SUB>) for the parabolic inhibition type in this study. Kinetic measurements and spectrofluorimetry studies showed that FA induced regional changes in the active site of tyrosinase. One possible binding site for FA was identified under the condition without L-DOPA. The computational docking simulations further revealed that FA can interact with HIS263 and HIS85 at the active site. Furthermore, four important hydrogen bonds were found to be involved with the docking of FA on tyrosinase. Our study provides insight into the mechanism by which dicarboxylic acids such as FA inhibit tyrosinase. By inhibiting tyrosinase and its central role in pigment production, FA is a potential natural antipigmentation agent.

Preventive Effects of Intracisternal Alphatochopherol on Cerebral Vasospasm in Experimental Subarachnoid Hamorrhage

SerdarKemalo?lu,Umit?zkan,FahriYilmaz,ErdemAK,HamitAcemo?lu,G?n?l?lmez,Ramazan?ım?ek,AbdurrahmanBakir 연세대학교의과대학 2003 Yonsei medical journal Vol.44 No.6

Effects of L-malic acid on alpha-glucosidase: inhibition kinetics and computational molecular dynamics simulations.

Gou, Lin,Zhan, Yi,Lee, Jinhyuk,Li, Xuan,Lü,, Zhi-Rong,Zhou, Hai-Meng,Lu, Hang,Wang, Xi-Yao,Park, Yong-Doo,Yang, Jun-Mo Humana Press 2015 Applied biochemistry and biotechnology Vol.175 No.4

<P>The inhibitory effect of L-malic acid (MA) on alpha-glucosidase (EC 3.2.1.20) was investigated by combination study between inhibition kinetics and computational simulations. The results from the serial kinetics demonstrated that MA could directly inactivate the enzyme activity in a dose-dependent manner and a typical non-competitive type, as well as in a fast inactivate process without detectable time course. The tertiary conformation study with an application of spectrofluorimetry showed that MA modulated the tertiary structural conformation of alpha-glucosidase both on the overall and on regional active site pocket, which monitored by red-shift intrinsic fluorescence peak with decreases intensities, and the significant intensity increasing of 1-anilinonaphthalene-8-sulfonate (ANS)-binding fluorescence, respectively. To have more insight, we also adapted the computational molecular dynamics (MD) simulations. The results showed that MA was located in the entrance of active pocket for the catalytic reaction and blocked the passage of substrate. It confirmed that MA inhibits as a non-competitive type, not direct docking to the glucose binding site. Our study provides important molecular mechanisms to figure out alpha-glucosidase inhibition that might associate to development of type 2 diabetes mellitus drug.</P>

Lentinula edodes (Shiitake) Modulates Chemically Induced Mutagenesis by Enhancing Pitting

Patrı´cia L. Alves de Lima,Marina M. Sugui,Angela I.M. Petrı´cio,Lı´zia C. Vilela,Andre´a V.F. Pinto,Priscila R. Martins,Ramon Kaneno,Daniel A. Ribeiro,Daisy M.F. Salvadori,Lu´cia R. Ribeiro 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.8

This study was undertaken to understand how Lentinula edodes modulates in vivo mutagenesis induced by alkylating agents in bone marrow and peripheral blood as described in our previous article. Male Swiss mice were pretreated for 15 consecutive days with aqueous extracts prepared from L. edodes, after which, the number of circulating blood cells, normal erythroid bone marrow cell cycling, and phagocytosis of micronucleated reticulocyte (MNRET) and activation of spleen macrophages were assessed. The results indicate that the antimutagenicity seen in bone marrow and peripheral blood is exerted by distinct compounds with different actions. The antimutagenic effect in bone marrow is exerted by compounds subject to degradation at deep-freeze storage temperature of - 20 C. On the other hand, compounds responsible for antimutagenicity in peripheral blood are not subject to degradation at - 20 C. The results also indicate that the antimutagenic action in peripheral blood leading to the reduction of circulating MNRET occurs in the spleen primarily through a phagocytic activity due to higher macrophage numbers and probably not due to the enhanced activation state of individual cells.

Behaviour of flush end-plate beam-to-column joints under bending and axial force

Luís Simões da Silva,Luciano R. O. de Lima,Pedro C. G. da S. Vellasco,Sebastião A. L. de Andrade 국제구조공학회 2004 Steel and Composite Structures, An International J Vol.4 No.2

Steel beam-to-column joints are often subjected to a combination of bending and axial forces. The level of axial forces in the joint may be significant, typical of pitched-roof portal frames, sway frames or frames with incomplete floors. Current specifications for steel joints do not take into account the presence of axial forces (tension and/or compression) in the joints. A single empirical limitation of 10% of the beam’s plastic axial capacity is the only enforced provision in Annex J of Eurocode 3. The objective of the present paper is to describe some experimental and numerical work carried out at the University of Coimbra to try to extend the philosophy of the component method to deal with the combined action bending moment and axial force.

О некоторых особенностях выражения субьекта в конструкциях с полными страдательными причастиями в современном русском языке

Л.Ю.Юшина 서울대학교 러시아연구소 1999 러시아연구 Vol.9 No.1

고급 러시아어 과정에 들어선 외국인들이 피동분사를 학습하는 데 있어서 동사를 포함하는 문장과 같은 의미를 지니는 피동분사 구문을 파악하는 것은 매우 중요한 부분이다. 예 1) Он читал книгу, принесённую ему другом / которую принёс ему друг. 이 경우 외국인 러시아어 학습자들이 자주 범하게 되는 실수를 분석해 보면 이들이 조격으로 표시되는 행위 주체를 반드시 나타내야 한다고 생각하고 있음을 알 수 있게 된다. 그러나 사실상 그렇지 않다. 피동분사 구문에서 주체의 표시는 피동분사의 용법 유형과 의미론적 기능에 의해 결정된다. 두 가지 그러한 경우가 있다. 그 중 하나는 분사가 수식어를 지니는 객체에 대하여 행해진 행위를 지시하는 경우이다. 이 경우 분사의 행위 주체의 위치는 열린 상태가 되는데 이 경우 가장 일반적인 방법은 명사 조격으로 주체의 위치를 대체하는 것이다. (상기 예문) 이 경우 행위 주체의 표시는 필수적이다. 또 하나의 분사의 기능은 수식어에 의해 한정되는 대상의 특성을 표현하는 것이다. 예 2) Она была в вышитой сорочке. 이러한 기능으로 피동분사를 사용하는 경우, 즉 행위가 아닌, 행위에 의해 생겨난 속성을 표현하기 위한 경우, 행위 주체의 위치는 닫힌 상태가 되어 더 이상의 대체를 필요로 하지 않게 된다. 첫 번째 기능(행위를 표현하는 기능)에서 피동분사는 문장의 구조를 복잡화하면서 규칙적으로 술어의 의미를 보충한다. 이것은 문장 속에 술어로 표현된 행위 - 예 1)에서의 читал - 외의 또 다른 행위인 비술어적 행위(принесённую) 표현이 존재하기 때문이다. 두 번째 기능(속성 표현의 기능)에서는 피동분사가 형용사와 대등하게 연결되거나 단독으로(확대어 없이) 사용될 수 있는 것이 특징적이다. 예 3) В дверях мясных лавок висели красные, развороченные туши мёртвой говядины ; Мокрые цветы диких пионов горели на окне, как раскалённые угли. 피동분사가 속성을 표시하는 경우 사용된 조격 형태는 형식적으로 행위 주체와 유사하게 보이지만 사실상 행위의 주체를 표현하지 않으며 피동분사로 표현된 속성의 발생원을 지적한다. 예 4) Я вышел из Лувра на площадь, раскалённую сентябрьским полуденным солнцем. - 이 때 태양(солнце)은 원인이기는 하지만 적극적인 행위자는 아니다. 결론적으로, 피동분사의 용법은 해당 동사의 어휘 의미에 따라서 달라질 수 있다.

On the Security Aspects of Internet of Things: A Systematic Literature Review

Evandro L. C. Macedo,Egberto A. R. de Oliveira,Fabio H. Silva,Rui R. Mello Jr,Felipe M. G. França,Flavia C. Delicato,José F. de Rezende,Luís F. M. de Moraes 한국통신학회 2019 Journal of communications and networks Vol.21 No.5

Internet of Things (IoT) has gained increasing visibilityamong emerging technologies and undoubtedly changing our dailylife. Its adoption is strengthened by the growth of connected devices(things) as shown in recent statistics. However, as the numberof connected things grows, responsibility related to security aspectsalso needs to increase. For instance, cyberattacks might happenif simple authentication mechanisms are not implemented on IoTapplications, or if access control mechanisms are weakly defined. Considering the relevance of the subject, we performed a systematicliterature review (SLR) to identify and synthesize security issuesin IoT discussed in scientific papers published within a periodof 8 years. Our literature review focused on four main security aspects,namely authentication, access control, data protection, andtrust. We believe that a study considering these topics has the potentialto reveal important opportunities and trends related to IoTsecurity. In particular, we aim to identify open issues and technologicaltrends that might guide future studies in this field, thusproviding useful material both to researchers and to managers anddevelopers of IoT systems. In this paper, we describe the protocoladopted to perform the SLR and present the state-of-the-art on thefield by describing the main techniques reported in the retrievedstudies. To the best of our knowledge, ours is the first study tocompile information on a comprehensive set of security aspects inIoT. Moreover, we discuss the placement, in terms of architecturaltiers, for deploying security techniques, in an attempt to provideguidelines to help design decisions of security solution developers. We summarize our results showing security trends and researchgaps that can be explored in future studies.

Obesity and Breast Cancer: The Role of Crown-Like Structures in Breast Adipose Tissue in Tumor Progression, Prognosis, and Therapy

Sara Socorro Faria,Luís Henrique Corrêa,Gabriella Simões Heyn,Lívia Pimentel de SantAna,Raquel das Neves Almeida,Kelly Grace Magalhães 한국유방암학회 2020 Journal of breast cancer Vol.23 No.3

Obesity is associated with increased risk and aggressiveness of many types of cancer. Women with obesity and breast cancer are more likely to be diagnosed with larger and higher-grade tumors and have higher incidence of metastases than lean individuals. Increasing evidence indicates that obesity includes systemic, chronic low-grade inflammation, and that adipose tissue can act as an important endocrine site, secreting a variety of substances that may regulate inflammation, immune response, and cancer predisposition. Obesity-associated inflammation appears to be initially mediated by macrophage infiltration into adipose tissue. Macrophages can surround damaged or necrotic adipocytes, forming “crown-like” structures (CLS). CLS are increased in breast adipose tissue from breast cancer patients and are more abundant in patients with obesity conditions. Moreover, the CLS index-ratio from individuals with obesity seems to influence breast cancer recurrence rates and survival. In this review, we discuss the most recent cellular and molecular mechanisms involved in CLS establishment in the white adipose tissue of women with obesity and their implications for breast cancer biology. We also explain how CLS influence the tumor microenvironment and affect breast cancer behavior. Targeting breast adipose tissue CLS can be a crucial therapeutic tool in cancer treatment, especially in patients with obesity.

Modeling for the strap combined footings Part I: Optimal dimensioning

Gabriel Aguilera-Mancilla,Arnulfo Luévanos-Rojas,Sandra López-Chavarría,Manuel Medina-Elizondo 국제구조공학회 2019 Steel and Composite Structures, An International J Vol.30 No.2

This paper presents a new model for the strap combined footings to obtain the most economical contact surface on the soil (optimal dimensioning) to support an axial load and moment in two directions to each column. The new model considers the soil real pressure, i.e., the pressure varies linearly. Research presented in this paper shows that can be applied to the T-shaped combined footings and the rectangular combined footings. The classical model uses the technique of test and error, i.e., a dimension is proposed, and subsequently, the equation of the biaxial bending is used to obtain the stresses acting on each vertex of the strap combined footing, which must meet the conditions following: The minimum stress should be equal or greater than zero, and maximum stress must be equal or less than the allowable capacity that can withstand the soil. Numerical examples are presented to obtain the optimal area of the contact surface on the soil for the strap combined footings subjected to an axial load and moments in two directions applied to each column. Appendix shows the Tables 4 and 5 for the strap combined footings, the Table 6 for the T-shaped combined footings, and the Table 7 for the rectangular combined footings.