http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Vinblastine Determination Measured by a Sensitive ELISA Inhibition Assay
Song,Eun-Young,Chung,Tai-wha,Kim,Jae-Wha,Nam,Kyung-Soo,Han,Mi-Young,Choe,In-Seong,Lee,Hee-Gu THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1996 Journal of biomedical laboratory sciences Vol.2 No.1
Vinblastine을 포함하는 bis-indole alkaloids에 대한 단일클론 항체를 생산하여 Vinca alkaloids의 양을 측정할수 있는 간편한 immunoassay 체계를 확립하였다. Vinca alkaloids는 periwinkle식물체의 배양된 세포로 부터 추출하여 BSA와 접합한 후 Balb/c생쥐에 면역시켜 얻은 비장세포와 골수종양세포의 융합을 유도하여 VBL-BSA에 반응하는 클론을 ELISA방법으로 분석하였으며 이들 클론 중 bis-indole alkaloids와 특이적으로 반응하는 항체는 inhibition assay를 통하여 분리할 수 있었고 그 결과 두개의 단일클론 항체를 형성하는 세포주(KN-1과 KN-2)를 확립하였다. KN-1의 경우 dimeric bis-indole alkaloids 와는 상당한 교차반응을 나타낸 반면 monomeric bis-indole alkaloids와는 교차반응을 나타내지 않았으며 이 클론의 항체를 이용하여 배양된 세포 추출물에 포함된 Vinca alkaloids의 양을 측정한 결과 0.05nM정도의 dimeric Vinca alkaloids까지도 측정할 수 있었다. Specific monoclonal antibodies(mAbs) against bis-indole alkaloids related to vinblastine were established to develop a simple and specific immunoassay system for the quantitation of Vinca alkaloids. Vinca alkaloids were extracted from tissue cultured cells of periwinkle plant (Vinca rosea L.). Spleen cells from Balb/c mice immunized with vinblastine-bovine serum albumin(VBL-BSA) conjugate as immunogen were fused with myeloma cells(Sp2/0-Ag.14) in the presence of polyethylene glycol. In the preliminary experiments, 32 clones which highly reacted with VBL-BSA conjugate were selected by ELISA((Enzyme-linked immunosorbent assay). These clones were further analyzed by inhibition assay of ELISA. The results obtained with two typical monoclonal antibodies, KN-1 and KN-2, were described. KN-1 exhibited considerable reactivities with soluble dimeric bis-indole alkaloids, whereas no cross reacted with monomeric bis-indole alkaloids. However KN-2 showed cross reactivity with mono- and di-meric bis-indole alkaloids. Furthermore, KN-1 was applied to the immunoassay system for determining the VBL amounts of in vitro cultured cell extracts. This assay system could detect dimeric vinca alkaloid as low as 0.05 nM.
Reactivity of 7-Dithiocarboxy-imidazo [2,1-b]thiazolium-betnine with Aliphatic Alkylating Agents
Song, Jung-Wha,Suh, Myung-Eun,Yoo, Kyung-Ho,Park, Sang-Woo The Pharmaceutical Society of Korea 1989 Archives of Pharmacal Research Vol.12 No.1
We have reported earlier on the reactivity of 7-dithiocarboxy-3-phenyl-5,6-dihydro imidazo[2,1-b]thiazolium-betaine with several para-substituted phenacyl bromides. In this work reactions of 7-dithiocarboxy-3-phenyl(or methyl)-5,6-dihydro imidazo[2,1-b]thiazolium-betaine with a series of aliphatic alkylating agents of ${\alpha}$ -halo ketone,${\gamma}$-halo koto ester and ${\alpha}$ -halo ester were examined for the similar purpose. In case of ${\alpha}$-halo ketone or ${\gamma}$-halo koto ester such as ${\alpha}$ -chloro acetone or ethyl 4-chloro acetoacetate new biheterocyclic compound was obtained via ring transformation reaction. However, reaction of the betaine with methyl(or ethyl) bromoacetate used as a ${\alpha}$-halo ester, gave, in-stead, S-alkylated quarternary ammonium salt.
기경도 ( Kyung Do Ki ),변재용 ( Jae Yong Byun ),김상훈 ( Sang Hoon Kim ),송동화 ( Dong Wha Song ),권기환 ( Kee Hwan Kwon ),박문서 ( Moon Suh Park ) 대한산부인과학회 2009 Obstetrics & Gynecology Science Vol.52 No.4
목적: 임신 시 벨마비의 발생 빈도는 비임신 시에 비해 높게 보고되고 있다. 비임신 시와 비교하여 임신 시 발생한 벨마비의 발병 시기, 임상 양상, 회복률에 대해 알아보고자 하였다. 연구 방법: 1996년부터 2006년까지 임신 중 벨마비가 발생한 43명의 환자를 대상으로 하였으며 비임신 시 벨마비가 발생한 가임기 여성 61명을 대조군으로 의무기록을 토대로 후향적 연구를 시행하였다. 결과: 임신 중 벨마비는 평균 재태연령 32주에 발생하였으며, 81.4% Objective: Although Bell`s palsy is not common in pregnancy, it is more prevalent among pregnant women than among non-pregnant women. Since the exact clinical characteristics of this condition are not fully understood, we evaluated the epidemiology, pathop
송미경(Song Mi-Kyung),배순화(Bae Soon-Wha) 한복문화학회 2013 韓服文化 Vol.16 No.2
A seated Ksitigarbha in the Bogwangsa, Sokcho, Gangwon-do was enshrined in 1654 and known to be restored in 1740. The opened votive textile pieces from a wooden sculpture of the seated Ksitigarbha has studied aiming to identify whether it was originally stored in mid-17C. or being refilled during restoration of the seated Ksitigarbha, mid-18C. Comparative study was applied for conforming the stored year of opened votive textile with a control group, a chronically known range of excavated satin damask Various votive textiles are found from inside of the seated Kstigabha. It includes an unopened storage box (후령통) which is covered with the yellow silk tabby and some pieces of textiles, such as the dragon pattern satin with supplementary gold wefts, the four season flowers pattern of satin damask, as well as the green and blue silk threads. The dedicative inscription is found too. By the comparative study with chronically known excavated costumes, opened pieces of votive textiles from the seated Kstigabha are identified to be filled in 1654, thereby unopened votive box assumes to be filled while restoration in 1974. Also due to the color of votive textiles from the seated Kstigabha remains clear, it would be expected being a useful material to identify the chronical record of 17C’s satin damask.
김경란 ( Kyung Ran Kim ),최정화 ( Jeong Wha Choi ),이경숙 ( Kyung Suk Lee ),송은영 ( Eun Young Song ) 한국산업위생학회 2008 한국산업보건학회지 Vol.18 No.1
The objective of this study is to survey the risk factors of greenhouse vegetables farmers` work-related musculoskeletal disorders(MSDs). To offer the fundamental data for agricultural improvement of greenhouse vegetable farms, the questionnaire of MSDs and heath condition were carried out. A group of 736 workers(males=492, females=244) in 6 branches constitutes the population and database for the analysis between 2004 and 2005. The sample branch was chosen based on paper are watermelon, strawberry, tomato, melon, cucumber, lettuce. The results are as follows: 1. Smoking rates in cucumber and tomato is the highest. 2. Drinking rates in strawberry, cucumber and lettuce is the highest in males, watermelon and melon are the highest in females. 3. Regular exercise rates in melon are the highest. 4. Physical and mental fatigue in females is higher than that of males in most crops except melon. That of strawberry and tomato is the highest. 5. The prevalence rates of medical diagnosed diseases are higher in order of osteoarthritis, herniated nucleus pulposus(HNP), and hypertension. That of strawberry, melon, and cucumber is high in case of osteoarthritis; Strawberry, melon, and watermelon are high in case of HNP. 6. Prevalence rates of musculoskeletal symptoms among the various pain areas are higher in order of low back, knees, and shoulders. That of tomato and watermelon is high in case of low back; strawberry and tomato are high in case of knees. These results can be used practically for agricultural improvement of greenhouse vegetables farms to prevent MSDs.
정화순,나동진,도화섭,송경순,양동욱,윤형두,윤홍섭,이옹수,조한익,지현숙,한경자 대한임상검사정도관리학회 1996 臨床檢査와 精度管理 Vol.18 No.1
1995년에 전국 350여기관을 대상으로 5월, 8월, 11월 3회에 걸쳐서 실시한 혈액학 검사의 신빙도 조사결과는 다음과 같았다. 검사종목은 전년도와 같아 백혈구수, 혈색소, 혈소판수, 적혈구수, 망상 적혈구수, 백혈구 감별계산, 혈구형태판독의 7종목 이었다. 자동화기기의 사용은 전년도와 같이 대부분의 병원에서 (97.5%) 자동기기를 사용하고 있고 수동법은 검사의 신뢰도가 매우 낮으므로, 자동화기기로 전환이 요구된다. 대부분 검사의 평균변이계수의 변화추이를 볼 때 상당한 개선이 있어왔다. 결과기록의 오류나 관리검체 사용상의 미숙은 많이 줄어들었으나 이러한 결과가 전기관의 결과에 나쁜 영향을 끼치므로 세심한 주의가 요구된다. Three trials of external quality assessment in diagnostic hematology were performed in 1995 with 351 participitating laboratories in Korea. We performed quality assessment for complete blood cell count(white blood cell count, hemoglobin, red blood cell count, platelet count), reticulocyte count, white cell differential count, red blood cell morphology, Most laboratories (97.5%) made use of automated cell counter for CBC. The performance of quality assessment appeared to be gradually improved year by year.