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Sung, Eun-Sil,Park, Kyung-Jin,Lee, Seung-Hyun,Jang, Yoon-Seon,Park, Sang-Koo,Park, Yoo-Hoi,Kwag, Won-Jae,Kwon, Myung-Hee,Kim, Yong-Sung American Association for Cancer Research, Inc 2009 Molecular Cancer Therapeutics Vol.8 No.8
<P>The proapoptotic tumor necrosis factor-related apoptosis inducing ligand (TRAIL) receptors death receptor (DR) 4 and DR5 are attractive targets to develop the receptor-specific agonistic monoclonal antibodies (mAb) as anticancer agents because of their tumor-selective cell death-inducing activity. Here, we report a novel agonistic mAb, AY4, raised against human DR4 in mice. ELISA analysis revealed that AY4 specifically bound to DR4 without competition with TRAIL for the binding. Despite distinct binding regions of AY4 on DR4 from those of TRAIL, AY4 as a single agent induced caspase-dependent apoptotic cell death of several tumor types through the extrinsic and/or intrinsic pathways without substantial cytotoxicity to normal human hepatocytes. Further, the AY4-sensitive cells followed the same cell death characteristics classified as type I and type II cells by the response to TRAIL, suggesting that the cell death profiles in responses to DR4 and/or DR5 stimulation are determined by the downstream signaling of the receptor rather than the kind of receptor. Noticeably, AY4 efficiently induced cell death of Jurkat cells, which have been reported to be resistant to other anti-DR4 agonistic mAbs, most likely due to the unique epitope property of AY4. In vivo administration of AY4 significantly inhibited tumor growth of human non-small cell lung carcinoma preestablished in athymic nude mice. Conclusively, our results provide further insight into the DR4-mediated cell death signaling and potential use of AY4 mAb as an anticancer therapeutic agent, particularly for DR4-responsive tumor types.</P>
Comprehensive Approach to Identifying Pysical Markers of Sperm Freezability
Sung-Jae Yoon,Md Saidur Rahman,Woo-Sung Kwon,Amena Khatun,Ye-Ji Kim,Do-Yeal Ryu,Myung-Geol Pang 한국수정란이식학회 2016 한국수정란이식학회 학술대회 Vol.2016 No.10
Processes of cryopreservation consists of three steps: dilution with the extender/cooling (Step 1), addition of cryoprotectant (Step 2), and freezing/thawing (Step 3) and spermatozoa are exposed different kind of environment and stress in each step. We categorized sperm samples as good freezablitiy (GF), damaged by cryoprotectant (DCP), and damaged by freezing (DF) and identified characteristics of each group in different step of cryopreservation. In Step 2, DCP was significantly decreased in motility, rapid speed and increased in slow speed. DF was significantly decreased in only motility whereas there were no significant difference between GF and DF and significantly higher than DCP in Step 2. Motility, rapid, medium speed of all group were significantly decreased in Step 3 and GF was significantly higer than other groups. AR pattern of all groups were significantly increased in Step 3 whereas GF was significantly lower than other groups. Additionally AR pattern of DF was significantly increased in Step 2. F pattern of DF and DCP were significantly decreased in Step 3. There no difference of B pattern in whole process. Mitochondrial activity of DCP was significantly decreased in Step 2 and mtichondrial activity of all groups were significantly decreased in Step 3. However mtichondrial activity of GF was higher than other groups. Viability result shows same significant difference with mitochondrial pattern. The present study compared with various sperm parameters in different groups which has different freezability. We defined different two types of group that damaged from different step of cryopreservation. DF and DCP is mainly damaged in Step 3 and Step 2 respectively. The results of current study suggest that various sperm parameters can be used as physical markers in freezability.
S-94 A comparison of endoscopic treatments for rectal carcinoid tumors
( Sang Myung Yeo ),( Jae Kwang Lee ),( Hyun Soo Kim ),( Chang Keun Park ),( Jae Kwon Jung ),( Dae Jin Kim ),( Ji Hun Jang ) 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1
Background and Aims: Carcinoid tumor are slow growing neoplasms developing from enterochromaffin cells. The incidence of rectal carcinoid tumors has increased markedly and a recent study reported that the rectum is the most common site of carcinoid tumor in the gastrointestinal tract. This study aimed to compare the safety and efficacy of endoscopic mucosal resection (EMR) with endoscopic submucosal dissection (ESD) for rectal carcinoid tumor. Patients and Methods: Between January 2004 to December 2014, 153 patients undewent either EMR (n=85) or ESD (n=68) for rectal carcinoid tumors. The characteristics of the patients and tumors, the rate of complete resection and the rate of complications were analyzed retrospectively. Results: Baseline characteristics showed no significant difference between the 2 groups. Data on clinical outcomes also showed no significant difference between the 2 groups except the procedure time. The procedure time in EMR group was significantly shorter than in ESD group. Conclusions: Our study shows that the treatment of rectal carcinoid tumor with ESD is not superior to EMR.
Time Series Classification of Cryptocurrency Price Trend Based on a Recurrent LSTM Neural Network
Kwon, Do-Hyung,Kim, Ju-Bong,Heo, Ju-Sung,Kim, Chan-Myung,Han, Youn-Hee Korea Information Processing Society 2019 Journal of information processing systems Vol.15 No.3
In this study, we applied the long short-term memory (LSTM) model to classify the cryptocurrency price time series. We collected historic cryptocurrency price time series data and preprocessed them in order to make them clean for use as train and target data. After such preprocessing, the price time series data were systematically encoded into the three-dimensional price tensor representing the past price changes of cryptocurrencies. We also presented our LSTM model structure as well as how to use such price tensor as input data of the LSTM model. In particular, a grid search-based k-fold cross-validation technique was applied to find the most suitable LSTM model parameters. Lastly, through the comparison of the f1-score values, our study showed that the LSTM model outperforms the gradient boosting model, a general machine learning model known to have relatively good prediction performance, for the time series classification of the cryptocurrency price trend. With the LSTM model, we got a performance improvement of about 7% compared to using the GB model.
Sung-Jae Yoon,Yoo-Jin Park,Woo-Sung Kwon,Kyu-Hyun Jeong,Sae-Mi Yoo,El-Sayed A. Mohamed,Myung-Geol Pang 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1
The objective of this study was to examine the effect of various discontinuous Percoll washing conditions on sperm capacitation status and sperm survival. Frozen epididymal sperm samples from 3 bulls (0.5 ml plastic straws, 6% glycerol in egg yolk- Tris-glycerol extender) were thawed in 37℃ water bath for 1 min. To rule out individual variation, 3 sperm samples were mixed after thawing. The mixed samples then were randomly allocated to 12 treatment groups. Briefly, the spermatozoa were centrifuged for three different time lengths (10, 20, and 30 min) at two gravities (300 X g and 700 X g) through two concentrations of discontinuous Percoll density gradient of 1 ml 90%: 1 ml 45% Percoll and 2 ml 90%: 2 ml 45% Percoll to remove extender, debris, and dead spermatozoa. Sperm capacitation status and sperm survival were evaluated using combined Hoechst 33258 and chlortertracycline fluorescence staining assay. The acrosome reacted spermatozoa (AR pattern), uncapaciated spermatozoa (F pattern) and sperm survival were significantly correlated with centrifugation time (p< 0.01). Significantly decreased F pattern observed as centrifugal time increased. As centrifugal time increased, spermatozoa with F pattern decreased and spermatozoa showing AR pattern increased. Moreover, the dead spermatozoa were significantly stimulated in time-dependent manner. However, there were no significant differences in various force of centrifugation and Percoll volume. These results suggest that only centrifugation time significantly affects sperm capacitation status and sperm survival.
Acute Pulmonary Toxicity and Body Distribution of Inhaled Metallic Silver Nanoparticles
Kwon, Jung-Taek,Minai-Tehrani, Arash,Hwang, Soon-Kyung,Kim, Ji-Eun,Shin, Ji-Young,Yu, Kyeong-Nam,Chang, Seung-Hee,Kim, Dae-Seong,Kwon, Yong-Taek,Choi, In-Ja,Cheong, Yun-Hee,Kim, Jun-Sung,Cho, Myung-Ha Korean Society of ToxicologyKorea Environmental Mu 2012 Toxicological Research Vol.28 No.1
The purpose of this study was to determine the acute pulmonary toxicity of metallic silver nanoparticles (MSNPs, 20.30 nm in diameter). Acute pulmonary toxicity and body distribution of inhaled MSNPs in mice were evaluated using a nose-only exposure chamber (NOEC) system. Bronchoalveolar lavage (BAL) fluid analysis, Western blotting, histopathological changes, and silver burdens in various organs were determined in mice. Mice were exposed to MSNPs for 6 hrs. The mean concentration, total surface area, volume and mass concentrations in the NOEC were maintained at $1.93{\times}10^7$ particles/$cm^3$, $1.09{\times}10^{10}\;nm^2/cm^3$, $2.72{\times}10^{11}\;nm^3/cm^3$, and 2854.62 ${\mu}g/m^3$, respectively. Inhalation of MSPNs caused mild pulmonary toxicity with distribution of silver in various organs but the silver burdens decreased rapidly at 24-hrs post-exposure in the lung. Furthermore, inhaled MSNPs induced activation of mitogen-activated protein kinase (MAPK) signaling in the lung. In summary, single inhaled MSNPs caused mild pulmonary toxicity, which was associated with activated MAPK signaling. Taken together, our results suggest that the inhalation toxicity of MSNPs should be carefully considered at the molecular level.
Applications of capacitation status for litter size enhancement in various pig breeds
Kwon, Woo-Sung,Shin, Dong-Ha,Ryu, Do-Yeal,Khatun, Amena,Rahman, Md Saidur,Pang, Myung-Geol Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.6
Objective: Several studies have reported the development of new molecular methods for the prognosis and diagnosis of male fertility based on biomarkers aimed at overcoming the limitations of conventional male fertility analysis tools. However, further studies are needed for the field application of these methods. Therefore, alternative methods based on existing semen analysis methods are required to improve production efficiency in the animal industry. Methods: we examined the possibility of improving litter size in various pig breeds using combined Hoechst 33258/chlortetracycline fluorescence (H33258/CTC) staining. The correlation between field fertility and capacitation status by combined H33258/CTC staining in different ejaculates spermatozoa (n = 3) from an individual boar (20 Landrace, 20 Yorkshire, and 20 Duroc) was evaluated as well as overall accuracy. Results: The acrosome reacted (AR) pattern after capacitation (%) was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 75%, 75%, and 70% in Landrace, Yorkshire, and Duroc pigs, respectively. The difference (${\Delta}$) in AR pattern before and after capacitation was positively correlated with the litter size of Landrace, Yorkshire, and Duroc pigs and the overall accuracy was 80%, 65%, and 55% in Landrace, Yorkshire, and Duroc pigs, respectively. However, the difference (${\Delta}$) in capacitated (B) pattern before and after capacitation was negatively correlated with the litter size of Landrace pigs and the overall accuracy was 75%. Moreover, average litter size was significantly altered according to different combined H33258/CTC staining parameters. Conclusion: These results show that combined H33258/CTC staining may be used to predict male fertility in various breeds. However, the selection of specific efficiency combined H33258/CTC staining parameters requires further consideration. Taken together, these findings suggest that combined H33258/CTC staining may constitute an alternative method for predicting male fertility until such time as fertility-related biomarkers are further validated.