http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Kim Sang-Kyum (검색결과 669 건)
- 무료
- 기관 내 무료
- 유료
-
Kim, Min Keun,Kang, Tae Ho,Kim, Sung Kyum,Jeong, Yu Seok,Yun, Han Dae,Kim, Hoon The Korean Society for Applied Biological Chemistr 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.6
The rsmA gene was cloned from soft-rot bacterium Pectobacterium carotovorum subsp. carotovorum LY34 (Pcc LY34), and its role in pathogenicity was investigated by marker exchange mutagenesis. From a cosmid library of Pcc LY34 genomic DNA, a positive clone carrying the rsmA gene was selected, and the gene was cloned by polymerase chain reaction (PCR) amplification. The gene is 186 bp in size and encodes a protein of 62 amino acids with a predicted molecular mass of 6,839 Da. The calculated pI of the RsmA is 8.16. The phylogenetic tree showed that the RsmA of Pcc LY34 appeared genetically identical to the CsrA of Pectobacterium atrosepticum SCRI1043 (100% identity) and similar to the CsrA of Yersinia pestis KIM10+(98.3%). The gene was disrupted by the $Km^r$ gene, and the cells became mutated (i.e., $RsmA^-$ mutant). The pathogenicity test revealed that the disease rating of the $RsmA^-$ mutant only differed slightly from that of the wild type on a slice of potato tuber and a Chinese cabbage stalk. These results suggest that RsmA is not an essential factor for the pathogenicity of Pcc LY34 and that the rsmA gene of Pcc LY34 is not completely derepressed in the $RsmA^-$ mutant for virulence-related genes, contrary to the results of Erwinia carotovora subsp. carotovora $RsmA^-$ mutant, which proved hypervirulent for celery petioles. These results showed that the microenvironmental conditions of the host and/or strain of pathogen are important for the coordination of virulence gene expression.
-
Role of Glutathione Conjugation in 1-Bromobutane-induced Immunotoxicity in Mice
Sang Kyu Lee,Dong Ju Lee,Tae Won Jeon,Gyu Sub Ko,Se Hyun Yoo,Hyun Woo Ha,Mi Jeong Kang,Wonku Kang,Sang Kyum Kim,Tae Cheon Jeong 한국독성학회 2010 Toxicological Research Vol.26 No.2
Halogenated organic compounds, such as 1-bromobutane (1-BB), have been used as cleaning agents, agents for chemical syntheses or extraction solvents in workplace. In the present study, immunotoxic effects of 1-BB and its conjugation with glutathione (GSH) were investigated in female BALB/c mice. Animals were treated orally with 1-BB at 375, 750 and 1500 ㎎/㎏ in corn oil once for dose response or treated orally with 1-BB at 1500 ㎎/㎏ for 6, 12, 24 and 48 hr for time course. S-Butyl GSH was identified in spleen by liquid chromatography-electrospray ionization tandem mass spectrometry. Splenic GSH levels were significantly reduced by single treatment with 1-BB. S-Butyl GSH conjugates were detected in spleen from 6 hr after treatment. Oral 1-BB significantly suppressed the antibody response to a T-dependent antigen and the production of splenic intracellular interlukin-2 in response to Con A. Our present results suggest that 1-BB could cause immunotoxicity as well as reduction of splenic GSH content, due to the formation of GSH conjugates in mice. The present results would be useful to understand molecular toxic mechanism of low molecular weight haloalkanes and to develop biological markers for exposure to haloalkanes.
-
Kim, Sung-Whan,Han, Hoon,Chae, Gue-Tae,Lee, Sung-Hoon,Bo, Sun,Yoon, Jung-Hee,Lee, Yong-Soon,Lee, Kwang-Soo,Park, Hwon-Kyum,Kang, Kyung-Sun Wiley (John WileySons) 2006 Stem cells Vol.24 No.6
<P>Buerger's disease, also known as thromboangiitis obliterans, is a nonatherosclerotic, inflammatory, vasoocclusive disease. It is characterized pathologically as a panangiitis of medium and small blood vessels, including both arteries and adjacent veins, especially the distal extremities (the feet and the hands). There is no curative medication or surgery for this disease. In the present study, we transplanted human leukocyte antigen-matched human umbilical cord blood (UCB)-derived mesenchymal stem cells (MSCs) into four men with Buerger's disease who had already received medical treatment and surgical therapies. After the stem cell transplantation, ischemic rest pain suddenly disappeared from their affected extremities. The necrotic skin lesions were healed within 4 weeks. In the follow-up angiography, digital capillaries were increased in number and size. In addition, vascular resistance in the affected extremities, compared with the preoperative examination, was markedly decreased due to improvement of the peripheral circulation. Because an animal model of Buerger's disease is absent and also to understand human results, we transplanted human UCB-derived MSCs to athymic nude mice with hind limb ischemia by femoral artery ligation. Up to 60% of the hind limbs were salvaged in the femoral artery-ligated animals. By in situ hybridization, the human UCB-derived MSCs were detected in the arterial walls of the ischemic hind limb in the treated group. Therefore, it is suggested that human UCB-derived MSC transplantation may be a new and useful therapeutic armament for Buerger's disease and similar ischemic diseases.</P>
-
-
Determination of Insulin Signaling Pathways in Hepatocytes
Kim, Sang-Kyum Korean Society of ToxicologyKorea Environmental Mu 2005 Toxicological Research Vol.21 No.3
Diabetes is a major cause of morbidity and mortality, and associated with a high risk of atherosclerosis, and liver, kidney, nerve and tissue damage. Defective insulin secretion in pancreas and/or insulin resistance in peripheral tissues is a central component of diabetes. It is well established that, regardless of the degree of muscle insulin resistance, glucose levels in diabetic and non-diabetic individuals are determined by the rate of hepatic glucose production. Moreover recently studies using liver-specific insulin receptor knockout mice show the paramount role of the liver in insulin resistance and diabetes. Insulin exerts a multifaceted and highly integrated series of actions via its intracellular signaling systems. The first major section of this review defines the major insulin-mediated signaling pathways including phosphatidylinositol 3-kinase and mitogen activated protein kinases. The second major section of the review presents a summary and evaluation of methods for determination of the role and function of signaling pathways, including methods for determination of kinase phosphorylation, the use of pharmacological inhibitors of kinase and dominant-negative kinase constructs, and the application of new RNA interference methods.
-
Kim, Choong-Yong,Yang, Byung-Chul,Kim, Joon-Kyum,Kim, Jong-Choon,Kim, Yong-Beom,Kang, Boo-Hyon,Han, Sang-Seop Korean Society of ToxicologyKorea Environmental Mu 2004 Toxicological Research Vol.20 No.4
The toxicity of CKD-602 was investigated at doses of 3, 9, and 27 mg/kg in rats, when the same total dose of CKD-602 was administered over 24 hr-continuous infusion or bolus injection. At 3 and 9 mg/kg, the 24-hr infusion group showed a more decreased WBC count on day 3, compared with the bolus group. Administration of CKD-602 caused more toxic effects such as the significant decreases of RBC counts, hematocrit, hemoglobin, and platelet count on day 7 post-administarion in the 24-hr infusion group than in the bolus group. Administration of CKD-602 also caused histopathological changes such as extramedullary hemopoiesis of liver and spleen, hyperplasia of femoral bone marrow, and caecal dilation. These toxic effects were more severe in the 24-hr infusion group than in the bolus injection group, indicating that the toxicity of CKD-602 may be dependant upon the duration of administration.
-
-
IRF7 promotes glioma cell invasion by inhibiting AGO2 expression.
Kim, Jun-Kyum,Jin, Xiong,Ham, Seok Won,Lee, Seon Yong,Seo, Sunyoung,Kim, Sung-Chan,Kim, Sung-Hak,Kim, Hyunggee Saikon Pub. Co 2015 TUMOR BIOLOGY Vol.36 No.7
<P>Interferon regulatory factor 7 (IRF7) is the master transcription factor that plays a pivotal role in the transcriptional activation of type I interferon genes in the inflammatory response. Our previous study revealed that IRF7 is an important regulator of tumor progression via the expression of inflammatory cytokines in glioma. Here, we report that IRF7 promotes glioma invasion and confers resistance to both chemotherapy and radiotherapy by inhibiting expression of argonaute 2 (AGO2), a regulator of microRNA biogenesis. We found that IRF7 and AGO2 expression levels were negatively correlated in patients with glioblastoma multiforme. Ectopic IRF7 expression led to a reduction in AGO2 expression, while depletion of IRF7 resulted in increased AGO2 expression in the LN-229 glioma cell line. In an in vitro invasion assay, IRF7 overexpression enhanced glioma cell invasion. Furthermore, reconstitution of AGO2 expression in IRF7-overexpressing cells led to decreased cell invasion, whereas the reduced invasion due to IRF7 depletion was rescued by AGO2 depletion. In addition, IRF7 induced chemoresistance and radioresistance of glioma cells by diminishing AGO2 expression. Finally, AGO2 depletion alone was sufficient to accelerate glioma cell invasion in vitro and in vivo, indicating that AGO2 regulates cancer cell invasion. Taken together, our results indicate that IRF7 promotes glioma cell invasion and both chemoresistance and radioresistance through AGO2 inhibition.</P>
-
Intrasellar Germinoma : A form of Ectopic Pinealoma Case Report
Kim, Hyung Dong,Park, Choong Kyum,Lee, Sang Ho,Ok, Yung Chul,Lee, Kyu Woong 대한신경외과학회 1976 Journal of Korean neurosurgical society Vol.5 No.2
생식기 계통에서 발생하는 정상피종(seminoma) 또는 난소정상피종(dysgerminoma)과 조직학적으로 유사한 종양이 두개강 내에서 발생 할 때를 비록이성기형종(atypical teratoma) 또는 배아종(germinoma)이라 한다. 이 종양은 주로 송과선 주위에서 잘 발생하나 가끔 송과체 이외의 장소에서도 발생되는데 이 때를 변위성송과체종(ectopic pinealoma)이라 하며 대부분의 변위성송과체종은 터키안 상부에서 발생되나 드물게는 터키안 내에서도 발생된다. 최근 국립의료원 신경외과에서는 15세 된 남아가 입원 약 2년 전부터 뇌압상승증상과 시상하부, 사구체 및 뇌하수체 침범증상을 호소하여 검사 후 수술한 결과 국내에서는 처음으로 송과체종양을 동반한 터키안 내 배아종을 확인하였기에 문헌고찰과 함께 보고한다. A case of ectopic pinealoma in the region of the sellar turcica, which was not enlarged reaiologically, so-called the intrasellar germinoma is described with its clinical manifestions, roentgenographic pictures, pathological findings and therapeutic points of view. A case of germinoma involving the pituitary gland and the hypothalamus with no conspicuous changes in visual field have been described. This tumor also combined with another tumor at the pineal region. This remarkable feature of extensive intrasellar involvement by a germinomma, generally considered to be a suprasellar tumor, has not been adequately emphasized previously in Korea.
-
Determination of Insulin Signaling Pathways in Hepatocytes
Sang Kyum Kim 한국독성학회 2005 Toxicological Research Vol.21 No.3
Diabetes is a major cause of morbidity and mortality, and associated with a high risk of atherosclerosis, and liver, kidney, nerve and tissue damage. Defective insulin secretion in pancreas and/or insulin resistance in peripheral tissues is a central component of diabetes. It is well established that, regardless of the degree of muscle insulin resistance, glucose levels in diabetic and non-diabetic individuals are determined by the rate of hepatic glucose production. Moreover recently studies using liver-specific insulin receptor knockout mice show the paramount role of the liver in insulin resistance and diabetes. Insulin exerts a multifaceted and highly integrated series of actions via its intracellular signaling systems. The first major section of this review defines the major insulin-mediated signaling pathways including phosphatidylinositol 3-kinase and mitogen activated protein kinases. The second major section of the review presents a summary and evaluation of methods for determination of the role and function of signaling pathways, including methods for determination of kinase phosphorylation, the use of pharmacological inhibitors of kinase and dominant-negative kinase constructs, and the application of new RNA interference methods.
DBpia연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
