[PD-0011] Functional study of CTP synthases during early seed development in rice

Jiyoung Kim(Jiyoung Kim),Sunghuyun Kwon(Sunghuyun Kwon),Gynheung An(Gynheung An),Jinmi Yoon(Jinmi Yoon),Lae-Hyeon Cho(Lae-Hyeon Cho) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

Areca nut exposure increases secretion of tumor‐promoting cytokines in gingival fibroblasts that trigger DNA damage in oral keratinocytes

Illeperuma, Rasika P.,Kim, Do Kyeong,Park, Young Jin,Son, Hwa Kyung,Kim, Jue Young,Kim, Jinmi,Lee, Doo Young,Kim, Ki‐,Yeol,Jung, Da‐,Woon,Tilakaratne, Wanninayake M.,Kim, Jin Alan R. Liss, Inc 2015 International journal of cancer Vol.137 No.11

<▼1><P>Molecular crosstalk between cancer cells and fibroblasts has been an emerging hot issue in understanding carcinogenesis. As oral submucous fibrosis (OSF) is an inflammatory fibrotic disease that can potentially transform into squamous cell carcinoma, OSF has been considered to be an appropriate model for studying the role of fibroblasts during early stage carcinogenesis. In this sense, this study aims at investigating whether areca nut (AN)‐exposed fibroblasts cause DNA damage of epithelial cells. For this study, immortalized hNOF (hTERT‐hNOF) was used. We found that the levels of GRO‐α, IL‐6 and IL‐8 increased in AN‐exposed fibroblasts. Cytokine secretion was reduced by antioxidants in AN‐exposed fibroblasts. Increase in DNA double strand breaks (DSB) and 8‐oxoG FITC‐conjugate was observed in immortalized human oral keratinocytes (IHOK) after the treatment of cytokines or a conditioned medium derived from AN‐exposed fibroblasts. Cytokine expression and DNA damage were also detected in OSF tissues. The DNA damage was reduced by neutralizing cytokines or antioxidant treatment. Generation of reactive oxygen species (ROS) and DNA damage response, triggered by cytokines, were abolished when NADPH oxidase (NOX) 1 and 4 were silenced in IHOK, indicating that cytokine‐triggered DNA damage was caused by ROS generation through NOX1 and NOX4. Taken together, this study provided strong evidence that blocking ROS generation might be a rewarding approach for cancer prevention and intervention in OSF.</P></▼1><▼2><P><B>What's new?</B></P><P>Fibroblasts in the tumor microenvironment influence tumor initiation and growth and are of particular interest in oral submucous fibrosis (OSF), a progressive fibrotic disease of malignant potential. This study shows that the release of tumor‐promoting cytokines by fibroblasts exposed to areca nut, the primary cause of OSF, induce DNA damage in oral keratinocytes. The findings suggest that fibroblasts indirectly promote epithelial transformation in OSF by secreting cytokines, whereby DNA damage of epithelial cells is inflicted by reactive oxygen species generated <I>via</I> NADPH oxidases. These insights could inform the development of new therapeutic approaches for OSF.</P></▼2>

알코올리즘 가족력이 있는 한국인 사회적 음주자에서 심리적 스트레스가 타액 코티졸 농도에 미치는 영향

서유나(Yuna Seo),김성곤(Sung-Gon Kim),민은정(Eun-Jeong Min),김진미(Jinmi Kim),박문일(Moon-Il Park),김수연(Soo-Yeon Kim),김대욱(Dae-Wook Kim),변원탄(Won-Tan Byun),김지훈(Ji-Hoon Kim) 대한생물치료정신의학회 2012 생물치료정신의학 Vol.18 No.2

Objectives:A number of studies have shown dysregulation of the hypothalamus-pituitary-adrenal axis in alcoholics. The aim of the present study was to determine whether there are differences in salivary cortisol responses to a psychological stressor between nonalcoholic offspring from families with a history of alcoholism and those without a family history of alcoholism in Korea. Methods:We studied 45 healthy social drinkers, who were medical students aged≥18 years. The participants completed a baseline assessment and, salivary cortisol levels were obtained. All subjects were exposed to almost 15 minutes of stress. Immediately following completion of the stress exposure, four additional salivary specimens were obtained for cortisol levels at 15 minute intervals. The subjects were divided into two groups those who reported a history of alcoholism in either parent(FHP, n=9) and those who reported no alcoholism in their family for three generations(FHN, n=30). Results:A significant difference in salivary cortisol by family history group(p<.001), and by time was discovered (p=.005) with a repeated-measures ANOVA. Basal salivary cortisol levels in FHP subjects were significantly higher than those in FHN subjects(p=.003). After stress exposure, salivary cortisol levels decreased significantly over time in the FHN group, whereas they did not in the FHP group. Conclusion:FHP subjects had higher baseline salivary cortisol concentrations than those of FHN subjects and maintained the levels after stress. This finding suggests that social drinkers with a history of alcoholism in a parent have increased vulnerability to stress and difficulty coping with stress effectively.

Ctbp2-mediated β-catenin regulation is required for exit from pluripotency

Kim, Tae Wan,Kwak, Sojung,Shin, Jihoon,Kang, Byung-Hee,Lee, Sang-Eun,Suh, Min Young,Kim, Jae-Hwan,Hwang, In-Young,Lee, Jong-Hyuk,Choi, Jinmi,Cho, Eun-Jung,Youn, Hong-Duk Nature Publishing Group 2017 Experimental and molecular medicine Vol.49 No.10

<P>The canonical Wnt pathway is critical for embryonic stem cell (ESC) pluripotency and aberrant control of β-catenin leads to failure of exit from pluripotency and lineage commitments. Hence, maintaining the appropriate level of β-catenin is important for the decision to commit to the appropriate lineage. However, how β-catenin links to core transcription factors in ESCs remains elusive. C-terminal-binding protein (CtBP) in <I>Drosophila</I> is essential for Wnt-mediated target gene expression. In addition, Ctbp acts as an antagonist of β-catenin/TCF activation in mammals. Recently, Ctbp2, a core Oct4-binding protein in ESCs, has been reported to play a key role in ESC pluripotency. However, the significance of the connection between Ctbp2 and β-catenin with regard to ESC pluripotency remains elusive. Here, we demonstrate that C-terminal-binding protein 2 (Ctbp2) associates with major components of the β-catenin destruction complex and limits the accessibility of β-catenin to core transcription factors in undifferentiated ESCs. <I>Ctbp2</I> knockdown leads to stabilization of β-catenin, which then interacts with core pluripotency-maintaining factors that are occupied by Ctbp2, leading to incomplete exit from pluripotency. These findings suggest a suppressive function for Ctbp2 in reducing the protein level of β-catenin, along with priming its position on core pluripotency genes to hinder β-catenin deposition, which is central to commitment to the appropriate lineage.</P>

Study on nucleic acid metabolic pathways during early seed development to improve rice grain quality

Jinmi Yoon,Cheol Woo Min,Jiyoung Kim,Sun-Tae Kim,Lae-Hyeon Cho 한국육종학회 2023 한국육종학회 공동학술발표집 Vol.2023 No.-

Localization of KEMI::lacZ Fusion Protein in Yeast Cells

Kim, Jinmi,Fink, Gerald R 충남대학교 생물공학연구소 1996 생물공학연구지 Vol.4 No.-

Sacharomyces cerevisiae의 KEM1 유전자는 세포의 영양 상태에 따라 spindle pole body나 microtubules의 기능을 조절하는 것으로 알려져 있다. 이 유전자 산물의 세포내 분포 및 기능을 규명하기 위하여, KEMI::lacZ 융합 유전자를 제조하였다. 즉, 클론된 KEM1 유전자에 대장균의 β-galacatosidase 구조유전자를 갖는 mini-Tn10-LUK element를 무작위 삽입한 pool을 제조하고, 이를 분석하여 KEM1의 기능 부위가 약 3.5kb에 해당함을 확인하였고, KEM1의 기능이 살아있는 KEM1::lacZ 융합 유전자의 클론을 선별하였다. 이 클론을 β-galacatosidase 항체를 이용한 indirect immunofluorescence 방법으로 분석하여 KEM1::lacZ 융합 단백질이 핵주변에 위치함을 확인하였다. KEM1 is known to control the spindle pole body or microtubule function, probably in response to the cellular nutritional conditions in Saccharomyces cerevisiae. Transposon insertions were performed in the cloned KEM1 gene using mini-Tn10-LUK element carrying E. coli β-galactosidase structural gene. A collection of random Tn10-LUK insertions defined an approximately 3.5 kb region required for the KEM1 function. From this collection functional KEM1::lacZ protein fusions were identified. Indirect immunofluorescence using anti-β-galacatosidase antibodies localized the KEM1::lacz fusion protein to the periphery of the nucleus.

Analysis of phenothrin and span80 on fire debris to determine a fire caused by bug sprays

Jinmi Jung(정진미),Siwon Kim,Kiwoong An,Kim Taeheui,Min-Hui Son,Mia Kwon 한국분석과학회 2021 학술대회논문집 Vol.2021 No.11

Sometimes unexpected fire accidents happen when they use a bug spray and an electric flyswatter to swat mosquitoes in summer season. The propellant of a bug spray is mainly LPG(liquefied petroleum gas, propane, isobutane and butane) in the aerosol mixture with the active ingredient such as insecticide. The aerosol mist can ignite to form an explosion mixture with oxygen in air and this explosion can be achieved by only one spark by electric flyswatter. Accidents are most likely to occur if LPG and air mixture which is within explosion range come into direct contact with an ignition source, such as a flame, pilot light or spark from electrical appliance that cycle on and off (e.g. refrigerator, air conditioner, vacuum cleaner, electric flyswatter, etc). Therefore we had to research to find major analyte from evidence (e.g. fire debris) gathered from the scene of these accidents. We collected several kind of commercial bug sprays and looked around their ingredients. Phenothrin and span80 were picked out as analytes because they were included among the several sprays and remained on fire debris, and analyzed by GC-MSD easily. Phenothrin is a cyclopropanecarboxylate ester used as a pyrethroid insecticide and span80(sorbitan monooleate) is one of the nonionic surfactants. The fire debris was extracted by hexane and carried out transesterification reaction by adding methanolic potassium hydroxide solution and ultrasonication. Then reaction mixure was vaporized with nitrogen and carried out silylation reaction by adding BSTFA and heating. The residue was analyzed by GC-MSD. We confirmed phenothrin and the silylation derivatives of span80(1,4-sorbitan-(TMS)4 and oleic acid-TMS).

A facile preparation route of n-type carbon buckypaper and its enhanced thermoelectric performance

Kim, Jinmi,Kwon, O Hwan,Kang, Young Hun,Jang, Kwang-Suk,Cho, Song Yun,Yoo, Youngjae Elsevier 2017 Composites science and technology Vol.153 No.-

<P><B>Abstract</B></P> <P>In this study, we prepared a thermoelectric device with p-type organic thermoelectric hybrid film and n-type buckypaper. Both the film and buckypaper are made of a hybrid filler of graphite nanoplatelets (GNPs) and single-walled carbon nanotubes (SWNTs). The p-type thermoelectric hybrid film has a free-standing form and enhanced thermoelectric performance owing to a polyvinylidene fluoride/carbon hybrid film purified with an acid solution, which successfully eliminates amorphous carbon, additives, and impurities. The n-type thermoelectric buckypaper was made with a filtration method with GNPs and SWNTs to the membrane filter. To convert this to an n-type thermoelectric property, a polyethyleneimine solution was also added by filtration process. In addition, adding sodium dodecyl benzene sulfonate during carbon dispersion enhanced thermoelectric performance, which was confirmed by measuring the electrical conductivity and Seebeck coefficient. A thermoelectric device using silver electrodes was produced with the thermoelectric composite film and buckypaper to verify its thermoelectric voltage and generating power.</P>

Acute Myocarditis in Children: a 10-year Nationwide Study (2007–2016) based on the Health Insurance Review and Assessment Service Database in Korea

Jinmi Kim,Min-Jung Cho 대한심장학회 2020 Korean Circulation Journal Vol.50 No.11

Background and Objectives: We performed a nationwide study to assess the incidence, treatment patterns, and outcomes of acute myocarditis in Korean children. Methods: The nationwide incidence, treatment patterns, and outcomes of acute myocarditis in Korean children were assessed using data between 2007 and 2016 from the Health Insurance Review and Assessment database. Results: We investigated 1,627 children during the study period. The overall incidence of acute myocarditis was 1.4 per 100,000 children in 2007 and 2.1 per 100,000 children in 2016, which indicates a significant increase in the trend over time. A bimodal age distribution was observed with a larger peak in infancy and a smaller peak in the mid-teenage years. No sex difference was observed in the incidence rate of acute myocarditis in children aged ≤5 years (373 boys vs. 366 girls); however, the incidence rate of acute myocarditis in adolescents aged ≥13 years showed significant male preponderance (482 boys vs. 152 girls). Acute fulminant myocarditis occurred in 371 children (22.8%) who needed extracorporeal membrane oxygenation and/or mechanical ventilator support. Of the 371 children with acute fulminant myocarditis, 258 (69.5%) survived. The survival rate of children with acute fulminant myocarditis remained nearly identical over the 10-year study period. Conclusions: This was the first nationwide epidemiological study to investigate acute myocarditis in Korean children. In our view, this study would help clinicians in decision-making and planning for optimal management of acute myocarditis in children.

Studies on KEM1 Gene Controlling Mitotic Cell Division in Yeast : Molecular Cloning of a High Copy Suppressor (ROK1) of kem1 kem1의 High Copy Suppressor (ROK1) 클로닝

Kim, Sanghyeon,Kim, Jinmi 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-

Saccharomyces cerevisiae에서 KEMI 유전자는 세포분열시 microtubules 과 spindle pole body 구조체의 기능에 관여하는 것으로 알려져 있다. KEMI 과 유사하거나 연관이 있는 기능을 갖는 새로운 유전자들을 찾는 목적으로, keml 돌연변이의 high copy suppressor 유전자, ROKI 를 찾아 냈다. ROKI 은 high copy 플라스미드에 클로닝되었을 때 keml 을 suppression 하고, low copy 플라스미드에서는 suppression 하지 않는다. keml 돌연변이의 benomyl 에 대한 민감성과 Kar^- enhancing 표현형을 동시에 suppression 하는 두개의 클론을 분리했으며, 제한효소로 분석했을 때 9.0 kb 의 insert 를 지닌 동일한 클론이었다. 이 suppressor 유전자 ROKI 의 제한지도를 작성하였고, 그 결과 KEMI 이 아닌 다른 유전자인 것으로 나타났다. Suncloning 실험으로 ROKI 은 적어도 3.0 kb 의 기능부위를 갖음을 확인했다. The KEMI gene is known to affect microtubule and spindle pole body function during the cell division cycle in Saccharomyces cerevisiae. To identify new genes with functions similar or related to those of KEMI, we isolatd a high copy suppressor gene (ROKI) that suppresses the keml mutation when cloned on a high copy number plasmid but not on a low copy number plasmid. Two clones which suppress both the benomyl hypersensitivity and the Kar^- enhancing phenotype of keml null mutation were isolated and were shown to have a 9.0 kb identical insert by restriction endonuclease analysis. The restriction map constructed indicates that this suppressor gene, ROKI, is not KEMI. Subcloning experiments suggest that the functional region of ROKI is at least 3.0 kb in size.