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GATA25, a novel regulator, accelerates the flowering time of Arabidopsis thaliana
Kim Kihwan,Lee Jooeun,Kim Byeonggyu,Shin Juhyung,Kang Tae-An,Kim Won-Chan 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.2
Intrinsic and exogenous signals in conjunction precisely regulate the initiation of flowering. Both signals influence flowering time, which is an integral part of plant reproduction. The signals converge through different pathways, and their coordinated action leads to the onset of flowering. Genetic pathways related to the regulation of flowering time are well-known from research into the molecular genetics of Arabidopsis thaliana. Specifically, crucial components of the photoperiodic pathway and floral integrators play a critically significant role in flowering. In this study, we found that GATA25 is a novel transcription factor that accelerates flowering time under long days. GATA25 encodes C-X2-C-X20-C-X2-C conserved cysteine residues of the zinc-finger domain and CCT domain which process photoperiodic flowering and regulate circadian rhythms. Flowering was accelerated by overexpression of GATA25 throughout the Arabidopsis thaliana. In contrast, GATA25 fused to SRDX (SUPERMAN repressive domain X)-motif plants showed delayed flowering. We also demonstrated that GATA25 induced the expression of floral integrator genes and photoperiodic pathway-related genes. Together, these results suggest that GATA25 might act to accelerate flowering time. Intrinsic and exogenous signals in conjunction precisely regulate the initiation of flowering. Both signals influence flowering time, which is an integral part of plant reproduction. The signals converge through different pathways, and their coordinated action leads to the onset of flowering. Genetic pathways related to the regulation of flowering time are well-known from research into the molecular genetics of Arabidopsis thaliana . Specifically, crucial components of the photoperiodic pathway and floral integrators play a critically significant role in flowering. In this study, we found that GATA25 is a novel transcription factor that accelerates flowering time under long days. GATA25 encodes C-X 2 -C-X 20 -C-X 2 -C conserved cysteine residues of the zinc-finger domain and CCT domain which process photoperiodic flowering and regulate circadian rhythms. Flowering was accelerated by overexpression of GATA25 throughout the Arabidopsis thaliana . In contrast, GATA25 fused to SRDX (SUPERMAN repressive domain X)-motif plants showed delayed flowering. We also demonstrated that GATA25 induced the expression of floral integrator genes and photoperiodic pathway-related genes. Together, these results suggest that GATA25 might act to accelerate flowering time.
HyMES에 대한 결합 확률 분포 기반 단일 파형 분석
박병규(ByeongGyu Park),김수리(Suhri Kim),김한빛(Hanbit Kim),진성현(Sunghyun Jin),김희석(HeeSeok Kim),홍석희(Seokhie Hong) 한국정보보호학회 2018 정보보호학회논문지 Vol.28 No.5
미래에 양자컴퓨터가 상용화될 것을 대비하여 전 세계적으로 양자컴퓨터에도 안전한 후 양자 암호(post quantum cryptography)에 대한 연구가 활발히 진행되고 있다. 그중 빠른 속도와 높은 안전성을 제공하는 부호기반 암호시스템에 대하여 다양한 부채널 분석에 대한 취약점이 발견되고 있으며, 이에 따라 부채널 분석에 안전한 암호시스템 설계를 위한 연구가 진행되고 있다. 본 논문에서는 HyMES(Hybrid McEliece Scheme)에 대해 단하나의 파형만을 이용하여 비밀키를 복원하는 방법을 제안한다. HyMES는 기존에 제안되었던 McEliece에 비해 키 크기가 작고, 암복호화 속도 또한 기존보다 빠르게 설계된 부호 기반 공개키 암호시스템이다. HyMES 복호화 알고리즘에는 신드롬 값 계산에 필요한 패리티 검사 행렬(parity-check matrix)을 연산하는 과정이 있다. 본 논문에서는 이 과정에서 사용되는 비선형 함수에 대한 결합 확률 분포가 비밀키 값에 따라 달라짐을 이용하여 HyMES를 분석하였다. 공개키 암호를 대상으로 한 결합 확률 분포 기반 분석은 본 논문에서 처음으로 제안되었다. The field of post-quantum cryptography (PQC) is an active area of research as cryptographers look for public-key cryptosystems that can resist quantum adversaries. Among those categories in PQC, code-based cryptosystem provides high security along with efficiency. Recent works on code-based cryptosystems focus on the side-channel resistant implementation since previous works have indicated the possible side-channel vulnerabilities on existing algorithms. In this paper, we recovered the secret key in HyMES(Hybrid McEliece Scheme) using a single power consumption trace. HyMES is a variant of McEliece cryptosystem that provides smaller keys and faster encryption and decryption speed. During the decryption, the algorithm computes the parity-check matrix which is required when computing the syndrome. We analyzed HyMES using the fact that the joint distributions of nonlinear functions used in this process depend on the secret key. To the best of our knowledge, we were the first to propose the side-channel analysis based on joint distributions of leakages on public-key cryptosystem.
가뭄 스트레스 특이적인 cis-regulatory element의 특성을 기반으로 한 신규 프로모터 구축
김기환 ( Kihwan Kim ),김병규 ( Byeonggyu Kim ),신주형 ( Juhyung Shin ),김원찬 ( Won-chan Kim ) 한국응용생명화학회(구 한국농화학회) 2021 Journal of Applied Biological Chemistry (J. Appl. Vol.64 No.1
가뭄은 작물의 성장과 생산성을 방해하는 비 생물학적 스트레스 중 하나다. 비 생물학적 스트레스에 대응하기 위해서는 식물이 불리한 환경 조건에서 스트레스에 나타내는 분자 조절 네트워크를 이해해야 한다. 비 생물학적 스트레스 (가뭄에 대응)에 대처할 수 있는 조합을 선별하기 위한 실험에서 스트레스 조건에서만 발현되는 5개의 가뭄 스트레스 유도성 프로모터를 선별하였으며, 이 중 36개의 cis-regulatory element를 선별하였다. 그 결과 가뭄 스트레스에서만 발현되는 유전자의 프로모터에서 cis-regulatory element를 새롭게 조합하여 미세 제어 조절을 할 수 있는 2 개의 합성프로모터(BL1, BL2)를 제작하였다. 합성프로모터를 포함한 형질전환식물(BL1-GUS, BL2-GUS)의 분석은 합성프로모터가 건조 조건에서 형질전환식물 내의 GUS유전자의 발현을 증가시키는 것을 통하여 확인하였다. 또한 Transient activation assay를 통해 DREB1A와 DREB2C에 의해 합성프로모터가 활성화되는 것도 확인하였다. 이러한 결과는 가뭄 특이적인 cis-regulatory element의 조합에 의해 제작한 합성프로모터가 다양한 비 생물학적 스트레스에 반응하고, 식물의 성장 지연을 유발하지 않고 스트레스에 효과적으로 대응할 수 있을 것이라 예상할 수 있다. Droughts are one of the abiotic stresses that hinders the growth and productivity of crop plants. Coping with abiotic stress is necessary to understand the molecular regulatory networks that makes plants respond to adverse environmental conditions. In our experiment to find a combination that can cope with abiotic stress (respond to drought), we screened 5 stressinducible promoters that are expressed only under stress conditions. This founded 36 cis-elements in stress-inducible promoters. With the result we designed 2 synthetic promoters (BL1, BL2) for fine-controlled regulation by assembling ciselements from the native promoters, which are expressed only under stress caused by droughts. Analysis of the transgenic plant (BL1-GUS, BL2-GUS) showed that the synthetic promoters increased the expression of β-glucuronidase (GUS) in transgenic plants under desiccation. Also in the transient activation assay demonstrated that synthetic promoters induced the cotransformation of effector DREB1A and DREB2C. These results expect that the synthetic promoter with a combination of droughtspecific elements can be used to respond to various abiotic stress and is resistant to stress without causing growth retardation.
Park, Byeonggyu,Kim, Hyeseon,Jeon, Taeck Joong Elsevier 2018 Biochemical and biophysical research communication Vol.499 No.4
<P><B>Abstract</B></P> <P>The small GTPase Ras proteins are involved in diverse cellular processes. We investigated the functions of RapC, one of 15 Ras subfamily GTPases in <I>Dictyostelium</I>. Loss of RapC resulted in a spread shape of cells; severe defects in cytokinesis leading to multinucleation; decrease of migration speed in chemoattractant-mediated cell migration, likely through increased cell adhesion; and aberrations in multicellular development producing abnormal multiple tips from one mound and multi-branched developmental structures. Defects in cells lacking RapC were rescued by expressing GFP-RapC in <I>rapC</I> null cells. Our results demonstrate that RapC, despite its high sequence homology with Rap1, plays a negative role in cell spreading and cell adhesion, in contrast to Rap1, which is a key regulator of cell adhesion and cytoskeleton rearrangement. In addition, RapC appears to have a unique function in multicellular development and is involved in tip formation from mounds. This study contributes to the understanding of Ras-mediated cellular processes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We characterized the functions of RapC, an isoform of Rap1 small GTPase. </LI> <LI> Loss of RapC causes defects in cytokinesis, cell migration, and development. </LI> <LI> RapC plays a negative role in cell spreading and cell adhesion. </LI> <LI> RapC is involved in tip formation from mounds during multicellular development. </LI> <LI> This contributes to the understanding of Ras-mediated cellular processes. </LI> </UL> </P>