Comparison of Proliferative Activity in Each Histological Subtypes of Benign and Atypical Intracranial Meningiomas by PCNA and Ki-67 Immunolabeling

최승진,장은덕,권성오,계대곤,박춘근,이상원,강준기,Choi, Seung Jin,Chang, Eun Deok,Kwon, Seung Oh,Kye, Dae Kon,Park, Choon Keun,Lee, Sang Won,Kang, Joon Ki The Korean Neurosurgical Society 2000 Journal of Korean neurosurgical society Vol.29 No.9

목 적 : 양성 뇌수막종에 비하여 이형성 및 악성 뇌수막종이 나쁜 임상적인 예후 및 양상을 보이는 것은 잘 알려져 있으나, 양성 뇌수막종에 있어서 각각의 병리조직학적 아형에 따른 생물학적 양상의 차이에 대해서는 잘 알려지지 않거나 일부 논란이 되고있다. 본 연구에서는 이형성 뇌수막종 및 양성 뇌수막종의 각각의 병리조직학적 아형에 따른 증식능의 차이여부를 알아보고자 PCNA와 Ki-67표지지수를 분석하였다. 방 법 : 본원에서 뇌수막종으로 수술을 시행하여 얻은, 재발을 보여 재수술을 시행한 2례를 포함하여, 파라핀에 포매시킨 27개의 조직을 대상으로 병리학적인 증식능을 분석하기 위해, PCNA에 대한 단일항체 및 MIB-1 단일항체를 이용한 면역조직화학적 염색을 시행하였다. 조직학적 분류상 meningothelial type이 8례, transitional type이 9례, fibroblastic type이 5례였으며, 이형성 수막종이 5례였다. 결 과 : PCNA표지지수의 평균값은 양성 수막종에서 meningothelial type이 $4.82{\pm}5.10%$, transitional type이 $9.01{\pm}4.25%$, fibroblastic type이 $5.66{\pm}5.32%$를 보였으나 이형성 수막종에서는 $27.62{\pm}19.67%$의 높은 지수를 나타냈고, Ki-67 표지지수의 평균값은 양성 수막종의 아형에서 각각 $0.43{\pm}0.85%$, $0.44{\pm}1.08%$, $0.24{\pm}0.18%$를 보이고, 역시 이형성 수막종에서는 $0.84{\pm}0.59%$의 높은 지수를 보였다. 즉, 양성 수막종에서 각각의 아형에 따른 PCNA 및 Ki-67 표지지수는 통계학적으로 의미있는 차이는 없었으나(p>0.05), 이형성 수막종에서는 의미있는 높은 표지지수를 보여(p<0.05) 양성 수막종에서 보다 높은 증식능을 보임을 알 수 있었다. 결 론 : PCNA 및 Ki-67 표지지수를 이용한 증식능의 비교결과, 양성 뇌수막종에서는 각각의 아형에 따른 생물학적 양상이나 예후는 차이가 없을것으로 생각되나, 이형성 수막종에서는 높은 증식능을 보여 이에 대한 예후를 예상할 수 있을것으로 생각되며, 또한 이러한 표지지수가 병리조직학적으로 양성과 이형성의 감별에 많은 도움이 될것으로 사료된다. Objective : The clinical prognosis and biological behavior of atypical and especially malignant meningiomas are well known to be worse than benign meningioma, but the degree of biological aggressiveness in each classical subtypes of benign meningioma is controversy. This study was performed to see whether there is a difference in the proliferative activity between each different histological subtypes of benign meningioma as well as atypical meningioma. Methods : Paraffin-embedded surgical specimens of 27 meningiomas, including two recurrent tumors, were studied to evaluate proliferative activity by immunohistochemical method with monoclonal antibodies to proliferating cell nuclear antigen(PCNA) and MIB-1. The specimens consisted of 8 cases of meningothelial, 9 cases of transitional, 5 cases of fibroblastic subtypes and 5 cases of atypical meningiomas. Results : Mean PCNA labeling indices of meningothelial, transitional and fibroblastic meningiomas were $4.82{\pm}5.10%$, $9.01{\pm}4.25%$ and $5.66{\pm}5.32%$, but that of atypical meningiomas was $27.62{\pm}19.67%$, noting a higher value compared to all three subtypes of benign meningiomas. Mean Ki-67 labeling indices of the above 3 subtypes were $0.43{\pm}0.85%$, $0.44{\pm}1.08%$ and $0.24{\pm}0.18%$, and that of atypical meningiomas was also revealed to be of higher value ($0.84{\pm}0.59%$). PCNA and Ki-67 labeling indices were not statistically different between histological subtypes of benign meningioma(p>0.05), but the differences of both immunolabeling between benign and atypical meningiomas were statistically significant(p<0.05). Conclusion : Immunolabeling of PCNA and Ki-67 in intracranial meningiomas reveals no prognostic difference between meningothelial, transitional and fibroblastic subtypes in classical benign meningiomas by measuring expression of PCNA and Ki-67, but it seems to be helpful in differentiating benign and atypical meningioma, later showing more proliferative activity and biological aggressiveness.

배양중 흰쥐 심장내피세포 단백합성체계의 미세구조에 미치는 Nocodazole과 Taxol의영향

장승원,박정현,성언기,이융창 대한해부학회 1996 Anatomy & Cell Biology Vol.29 No.1

Diagnostic Significance of Brainstem Auditory Evoked Potentials in Microvascular Decompression of Patients with Hemifacial Spasm or Trigeminal Neuralgia

( Sang-koo Park ),( Sung-hyuk Lim ),( Chan-woo Park ),( Jin-woo Park ),( Sung-ho Chang ),( Keun-hye Park ),( Hae-ja Park ),( Ji-hye Song ),( Dong-ok Uhm ),( Ki-bong Kim ) 대한임상검사과학회 2011 대한임상검사과학회지(KJCLS) Vol.43 No.1

The purpose of this study was to analyse brainstem auditory evoked potentials (BAEP) wave change data during microvascular decompression (MVD). The nerve function of Cranial Nerve VIII is at risk during MVD. Intraoperative monitoring of BAEP can be a useful tool to decrease the danger of hearing loss. Between January and December 2009, 242 patients had MVD for hemifacial spasm (HFS) and trigeminal neuralgia (TN). Among intraoperative BAEP changes, amplitude of V-V’ was the most frequently observed during cerebellar retraction and decompression step of the MVD procedure. 138 patients (57%) had no BAEP change while 104 patients (42.98%) had BAEP change. 69 patients (28.5%) had Type A-I, 16 patients (6.6%) had Type A-II, 5 patients (2.1%) had Type B, and 13 patients (5.37%) had Type C. MVD is a surgical procedure to relieve the symptoms (e.g. pain, muscle twitching) caused by compression of a nerve by an artery or vein. During BAEP intraoperative monitoring, the surgical step is important in interpreting the changes of wave V. Several potential mechanisms of injury may affect the cochlear nerve, and complete loss of BAEP is often associated with postoperative hearing loss. Intraoperative BAEP monitoring may provide an early warning of hearing disturbance after MVD.

고양이 소뇌 치상핵 자극에 따른 전경골근의 근전도 변화

성재훈,김문찬,강준기,최창락 대한신경외과학회 1994 Journal of Korean neurosurgical society Vol.23 No.7

Through the use of stereotactic neurosurgical operation, some authors have employed chronic electrical stimulation of the dentate nucleus reduce spasticity and each trial on spastic patients has show marked clinical improvement. We investigated the eletrophysiological changes of cats after stereotactic electrical stimulation of the dentate nucleus to elucidate the possibility of clinical application and to evaluate the clinical results The M-wave is an initial response and the F-wave is a late response in the electromyography of a muscle, which is stimulated by its original controlling nerve itself. There is evidence that the sue of the F-wave is dependent on motor neuron excitability and its amplitude is increased significantly at spastic condition. If such a relationship exists, procedures which are cam& out to relieve spasticity might be exerted to change the F-wave sue and thus it might be possible to use the F-wave as an objective monitor during electrical stimulation of the dentate nucleus. We investigated this possibility in experiments on 10 cats weighing betHieen 27kg and 4.4kg. We studied the change% of M and F waves, recorded in EMG, after stereotactic stimulation to cerebellar dentate nucleus in cats. The results were as follows 1) The change of mean value of M-wave amplitude was not significant(contro1 group, 3591 ± 1029μV stimulation group, 3424± 927μV, 00.05). 2) The mean value of F-wave amplitude was significantly reduced about 56.7% after the dentate stimulation(contro1 p u p , 443.2± 119μV stimulation p u p , 251.3±99.4μV, P<0.05). 3) The F/M ratio also significantly reduced about 56.8% after the dentate stimulation(contro1 group, 12.5±1.9%, stimulation group, 7.1± 1.2%, P<0.05). Our experimental results demonstrated that the dentate stimulation markedly decreased the sue of F-wave amplitude and F/M ratio in the experimental cats and we concluded that theseelectrophysiological changes can be applied as a parameter of clinical evaluation of electrical dentate stimulation for the spasticity.

Effects of DK-002, a synthesized (6aS,cis)-9,10-Dimethoxy-7,11b-dihydro-indeno[2,1-c]chromene-3,6a-diol, on platelet activity

Lee, Ki-Seon,Khil, Lee-Yong,Chae, Sang-Ho,Kim, Deukjoon,Lee, Byung-Hoon,Hwang, Gwi-Seo,Moon, Chang-Hyun,Chang, Tong-Shin,Moon, Chang-Kiu 이화여자대학교 약학연구소 2008 藥學硏究論文集 Vol.- No.17

In the present study, the mechanism of antiplatelet activity of DK.-002, a synthesized (6aS,cis)-9.10-Dimethoxy-7,11b-dihydro-indeno|2,1-c ]chroniene-3.6a-diol, was investigated. DK-002 inhibited the thrombin, collagen, and ADP-induced rat platelet aggregation in a concentration- dependent manner, with 1C_(50) values of 120. 27, and 47 μM, respectively. DK-002 also inhibited thrombin-induced dense granule secretion, thromboxane A_(2) synthesis, and [Ca^(2+)], elevation in platelets. DK-002 did not show any significant effect on ADP-induced inhibition of cyclic AMP elevation by prostaglandin E_(1). but DK-002 was confirmed to inhibit ADP-induced [Ca^(2+)] elevation and shape change. DK-002 inhibited 4- bronw-A23187-induced [Ca^(2+)], elevation in the presence of creatine phosphate/creatine phosphokinase (CP/CPK, a ADP scavenging system) and indomethacin (a specific inhibitor of cyclooxygenase). DK-002 also inhibited Ca^(2+) mobilization in thrombin- or 4-bromo-A23187-stimulated platelets through its inhibitory effects on both Ca^(2+) release from intracellular stores and Ca^(2+) influx, in the presence of CP/CPK and indomethacin. Taken together, the present study shows that DK-002 has inhibitory effects on stimulation of platelets, and suggests that its antiplatelet activity might be related to the inhibitory mechanism on Ca^(2+) mobilization in stimulated platelets. ⓒ 2005 Elsevier Inc. All rights reserved.

출산에 따른 그레이브스병의 재발 및 관련이자

임창훈,최현아,한승석,김해성,이창욱,정호연,한기옥,장학철,박원근,윤현구,한인권 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.2

Background: Pregnancy affects the course of Graves' Disease (GD), and patients who initially maintain euthyroid function into their middle trimester with minimum doses of antithyroid drugs become exacerbated after delivery. Even patients who are completely cured, requiring no treatment during pregnancy, can relapse after delivery. In this study, we examined the postpartum changes in the thyroid functions of patients with GD, and attempted to determine the factors contributing to these changes. Methods: The study subjects were recruited from pregnant women visiting our outpatient clinic for routine prenatal evaluations. 45 women previously diagnosed with GD, who had been treated and cured with hyperthyroidism, and were no longer taking any thyroid medications, were evaluated for 1 year post delivery. Results: Among 45 patients, 20 (44.4%) developed thyroid disorders following delivery. Postpartum thyroiditis (PPT) developed in 8 patients (17.8%), and GD developed in 12 (26.0%). The onset of the PPT disease 3.1 ± 1.4 months following delivery, which was significantly earlier than the 6.7 ± 2.7 months required for the post delivery onset of GD (p=0.003). The TBII values, measured during the thyrotoxic state in each womaen, were negative in women with PPT and positive in 71.4% of women with GD (p=0.030). The duration of treatment for hyperthyroidism prior or pregnancy, the number of recurrences, and the time interval without treatment, were not associated with the development of postpartum thyroid disorders. Whereas, the mean number of past pregnancies for women who developed PPT was 3.9 ± 2.1, and was significantly higher than the 2.2 ± 1.7 for women developing no thyroid dysfunctions (p=0.044). In 13 women their initial onset of GD occurred within one year postpartum, 7 (53.8%) having had a recurrence, which was significantly higher than in women whose disease onset occurred unrelated to delivery (5 of 32 women: 15.6%). Conclusion: Women with GD developed postpartum thyroid dysfunctions in 44.4% of cases. Women whose initial disease onset occurred within one year postpartum had higher recurrences of GD, and women who developed PPT had a history of higher gravidity compared to the euthyroid women postpartum. Therefore, if women with GD develop postpartum thyroid dysfunctions, the diagnosis should be made, and a treatment modality planned, following careful considerations of the patients' past obstetric history, changes in clinical manifestations and the TBII values

초초임계압(USC) 화력발전기술 개발

장성호(Sung Ho Chang),김범수(Bum Soo Kim),민택기(Taek Ki Min) 대한기계학회 2011 대한기계학회 춘추학술대회 Vol.2011 No.6

화력발전은 지구온난화의 주범으로 인식되고 있지만, 산업화와 지속적인 국가경제성장에 의한 전력소요 증가는 에너지사용을 증대시켜 에너지 차원의 부족을 초래하고 환경오염물질의 과다배출로 지구환경문제를 유발하고 있다. 에너지원의 확보를 위한 국가와 지역간 경쟁이 심화되어 새로운 갈등의 원인이 되고 있으며, 지구온난화와 에너지 문제를 해결하기 위해 교토의정서 등 기후변화 협약이 체결되고 화석에너지에 재생에너지로의 에너지원의 전환과 다양화가 추진되고 있지만, 각국의 이해관계와 기술부족으로 완벽한 해결책을 제시하지 못하는 상황이다. 에너지 부족을 해소하고 CO₂ 배출량을 저감할 수 있는 가장 효과적인 방안으로 기존 화력발전 효율을 향상시킨 고효율 발전과 Near Zero Emission수준의 저공해 기술이 결합된 고효율 석탄화력발전시스템을 개발현황을 논하고자 한다. For the environment and the limitation of the energy resource, the high efficiency power generation technology is necessary in the future. The Ultra Super Critical Power Generation technology is the key to manage the greenhouse gases and energy resource problems on Kyoto Protocol to the United Nations Framework Convention on Climate Change. Other countries and the manufacturer are trying to build the commercial power plant. In this paper, the efficient method of the Near Zero Emission is discussed the high efficient fossil power plant, Ultra Super Critical Power Generation. The ultra super critical power generation in Korea has been developed into two phases - Phase Ⅰ USC key technology development for 2002-08 and Phase Ⅱ USC development & optimizing technology in 2010-2017, that supported by the Korea Government.

Diagnostic Significance of Brainstem Auditory Evoked Potentials in Microvascular Decompression of Patients with Hemifacial Spasm or Trigeminal Neuralgia

Park, Sang-Koo,Lim, Sung-Hyuk,Park, Chan-Woo,Park, Jin-Woo,Chang, Sung-Ho,Park, Keun-Hye,Park, Hae-Ja,Song, Ji-Hye,Uhm, Dong-Ok,Kim, Ki-Bong 대한임상검사과학회 2011 대한임상검사과학회지(KJCLS) Vol.43 No.1

The purpose of this study was to analyse brainstem auditory evoked potentials (BAEP) wave change data during microvascular decompression (MVD). The nerve function of Cranial Nerve VIII is at risk during MVD. Intraoperative monitoring of BAEP can be a useful tool to decrease the danger of hearing loss. Between January and December 2009, 242 patients had MVD for hemifacial spasm (HFS) and trigeminal neuralgia (TN). Among intraoperative BAEP changes, amplitude of V-V' was the most frequently observed during cerebellar retraction and decompression step of the MVD procedure. 138 patients (57%) had no BAEP change while 104 patients (42.98%) had BAEP change. 69 patients (28.5%) had Type A-I, 16 patients (6.6%) had Type A-II, 5 patients (2.1%) had Type B, and 13 patients (5.37%) had Type C. MVD is a surgical procedure to relieve the symptoms (e.g. pain, muscle twitching) caused by compression of a nerve by an artery or vein. During BAEP intraoperative monitoring, the surgical step is important in interpreting the changes of wave V. Several potential mechanisms of injury may affect the cochlear nerve, and complete loss of BAEP is often associated with postoperative hearing loss. Intraoperative BAEP monitoring may provide an early warning of hearing disturbance after MVD.

캐스케이드 열펌프시스템의 운전 특성에 관한 연구

장기창(Ki-Chang Chang),백영진(Young-Jin Baik),나호상(Ho-Sang Ra),김지영(Ji-Young Kim),이재훈(Jea-Hun Lee) 한국지열·수열에너지학회 2009 한국지열에너지학회논문집 Vol.5 No.1

The purpose of this study is to investigate the performance of a water heat source cascade heat pump system R717(Ammonia) is used for a low-stage working fluid while R134a is for a high-stage. In order to gain a high temperature supply water in winter season, the system is designed to perform a cascade cycle. In this study, two experiments were carried out. One is a system starting test from the low load temperature of 10℃. The other is a system performance investigation over the R717 compressor capacity changes. Experimental results show that when it starts from the low load temperature, the suction temperature of the low-stage compressor is higher than that of a high-stage. The system performance increases when a water source temperature or a low-stage compressor rotational frequency goes higher.

혈중납이 흰쥐의 Microsomal Cytochrome P-450 Monooxygenase 활성도에 미치는 영향

박기현,장성근,윤영안,김기웅,박종안 순천향대학교 1993 논문집 Vol.16 No.3

In rats treated with lead nitrate and/or red ginseng extract mixture we observed a change in the level of hepatic cytochrome P-450 and electron transport system for P-450 dependent reaction, and content of Zincprotoporphyrin(ZPP) and Hemoglobin(Hb) in whole blood. The content of cytochrome P-450 and ?? , and the activity of ECHEase, AHHase and NADPH-cytochrome C(p-450) reductase were slowly decreased according to the dosage of lead nitrate. On the other hand, the activity of ?? reductase was increased according to the dosage of lead nitrate, but in case of treatment with red ginseng mixture, this enzyme activity was slightly decreased. In whole blood, the content of hemoglobin and ZPP were not shown any critival change but lead content increased according to dosage of lead nitrate. These results suggest that content and activity of cytochrome P-450-dependent monoxygenase may be dependent on specificity of substrates, and the acute exposure of lead nitrate and red ginseng extract mixture was not affected any biological significance.