Development of Long-Lasting Recombinant Human Albumin- Erythropoietin Fusion Protein in CHO

Ju-Yeop Shin,Chan-Hi Joung,Jin J. Lim,Myung-Hwan Kim,Sang-Min Lim,Hyung-Kwang Tan,Sang-Lin Kim,Jae-Kyoung Koo 한국생물공학회 2007 한국생물공학회 학술대회 Vol.2007 No.4

HCV core protein promotes liver fibrogenesis via up-regulation of CTGF with TGF-b1

Ju Yeop Shin,Wonhee Hur,Jin Sang Wang,Jeong Won Jang,Chang Wook Kim,Si Hyun Bae,Sung Key Jang,Se-Hwan Yang,Young Chul Sung,Oh-Joo Kwon,Seung Kew Yoon 생화학분자생물학회 2005 Experimental and molecular medicine Vol.37 No.2

Liver cirhosis is one of the major complications of hepatitis C virus (HCV) infection, but the me-chanisms underlying HCV-related fibrogenesis are stil not clear. Although the roles of HCV core protein remain poorly understood, it is supposed to play an important role in the regulation of celular growth and hepatocarcinogenesis. The aim of this study was to examine the role of HCV established an in vitro co-culture system with primary hepatic stellate cel (HSC) isolated from rats, and a stable HepG2-HCV core cell line which had been transfected with HCV core gene. The expressions of fibrosis-related molecules trans-forming growth factor β1 (TGF-β1), transforming growth factor β receptor II (TGFβRII), α-smoth muscle actin (α-SMA) and conective tisue growth factor (CTGF) were analyzed via histo-pression levels of matrix metaloprotinase-2 (MP- 2) and colagen type I (Col I) from the co-cultured media were measured by zymogram and ELISA, respectively. The expresions of α-SMA, TGF-β1, Col I, TGFβRII and MP-2 were significantly in-creased in the co-culture of stable HepG2-HCV core with HSC. Moreover, the significant increases of CTGF and TGF-β1 in the HCV core- expresing cels were observed by either Northern or Wes-tern blot analysis. These results sugest that HCV core protein may contribute to the hepatic fibro-via up-regulation of CTGF and TGF-β1.

Measurement of the Wear Amount of WC-coated Excavator Spacer using the PTA Process to Improve Wear Resistance by Using Reflective Digital Holography

Ju-Yeop Shin(신주엽),Hyeong-Jong Lim(임형종),Hang-Seo Lee(이항서),Han-Sub Kim(김한섭),Hyun-Chul Jung(정현철),Kyeong-suk Kim(김경석) 한국기계가공학회 2020 한국기계가공학회지 Vol.19 No.1

The spacer, which is located between the bucket and the arm of an excavator, has a role in preventing damage to the excavator arm during excavation work. When the durability of the spacer is increased, the lifetime of the arm can be extended and the processing costs can be reduced. To increase the durability of the spacer, tungsten carbide (WC) coating was applied on the surface of a spacer using the plasma transferred arc (PTA) process. The confirm the durability, a wear test using a pin-on disk type of wear testing machine was done under the given conditions and the wear amount on the surface of a tested specimen was measured using reflective digital holography. The results were compared with that of ALPHA-STEP.

Enhancement of in vivo Efficacy and Half-Life of Recombinant Human Albumins-Erythropoietin Fusion Protein

Ju-Yeop Shin,Chan-Hi Joung,Jin J. Lim,Myung-Hwan Kim,Sang-Min Lim,Hyung-Kwang Tan,Sang-Lin Kim,Jae-Kyoung Koo 한국생물공학회 2007 한국생물공학회 학술대회 Vol.2007 No.4

디지털 홀로그래피를 이용한 포토리소그래피 공정 제품 패터닝의 폭과 단차 측정

신주엽(Ju Yeop Shin),강성훈(Sung Hoon Kang),마혜준(Hye Joon Ma),권익환(Ik Hwan Kwon),양승필(Seung Pil Yang),정현철(Hyun Chul Jung),홍정기(Chung Ki Hong),김경석(Kyeong Suk Kim) 한국비파괴검사학회 2016 한국비파괴검사학회지 Vol.36 No.1

반도체 산업은 우리나라 주력산업중 하나로 매년 꾸준한 성장세를 보이며 지속적인 성장을 하고 있다. 이러한 반도체 산업에서의 중요한 기술은 소자의 고 집적화이다. 이는 면적당 메모리 용량을 증가시키는 것으로 핵심역할을 하는 것이 바로 포토리소그래피 기술이다. 포토리소그래피란 마스크의 표면에 빛을 쬐어 생기는 그림자를 웨이퍼 상에 인쇄하는 기술이며 반도체 제조공정에서의 가장 중요한 공정이다. 이러한 공정을 통해 나온 패터닝을 분석 시에 폭과 단차의 균일성을 측정한다. 이에 따라 본 논문은 포토리소그래피 공정이 적용된 시험편 패터닝에 폭과 판 사이와의 단차를 투과형 디지털 홀로그래피를 구성하여 측정하고자 한다. 투과형 디지털 홀로그래피 간섭계를 구성하고 시험편에 임의의 9포인트를 설정하여 각 포인트를 측정하고 상용장비인 SEM (scanning electron microscopy)과 alpha step으로 측정한 결과와 비교하고자 한다. 투과형 디지털 홀로그래피는 측정시간이 타 기법에 비에 짧다는 장점과 배율렌즈를 사용하기 때문에 저 배율에서 고 배율로 변경하여 측정할 수 있는 장점을 가지고 있다. 실험 결과로부터 투과형 디지털 홀로그래피가 포토리소그래피가 적용된 패터닝 측정에 유용한 기술임을 확인할 수 있었다. The semiconductor industry is one of the key industries of Korea, which has continued growing at a steady annual growth rate. Important technology for the semiconductor industry is high integration of devices. This is to increase the memory capacity for unit area, of which key is photolithography. The photolithography refers to a technique for printing the shadow of light lit on the mask surface on to wafer, which is the most important process in a semiconductor manufacturing process. In this study, the width and step-height of wafers patterned through this process were measured to ensure uniformity. The widths and inter-plate heights of the specimens patterned using photolithography were measured using transmissive digital holography. A transmissive digital holographic interferometer was configured, and nine arbitrary points were set on the specimens as measured points. The measurement of each point was compared with the measurements performed using a commercial device called scanning electron microscope (SEM) and Alpha Step. Transmission digital holography requires a short measurement time, which is an advantage compared to other techniques. Furthermore, it uses magnification lenses, allowing the flexibility of changing between high and low magnifications. The test results confirmed that transmissive digital holography is a useful technique for measuring patterns printed using photolithography.

The Impact of Discrepancy between Measured versus Stated Weight on Hemorrhagic Transformation and Clinical Outcomes after Intravenous Alteplase Thrombolysis

Shin, Dong Hoon,Kang, Min-Ju,Kim, Jin Wook,Shin, Dong-Jin,Park, Hyeon-Mi,Sung, Young Hee,Kim, Eung Yeop S. Karger AG 2017 Cerebrovascular diseases Vol.44 No.5

<P><B><I>Background:</I></B> An accurate measurement of patient weight is important in determining the dosage for intravenous alteplase thrombolysis. In most emergency rooms, however, weight is not measured. We investigated the difference between stated and measured weight and its effect on hemorrhagic transformation and clinical outcomes. <B><I>Methods:</I></B> We enrolled 128 consecutive patients who had hyperacute stroke and were treated by alteplase. Alteplase dose was calculated using the weight provided by patient or guardian/caregiver, and the actual weight was measured after administration. Patients were classified into 2 groups: overused group (stated weight >measured weight) and underused group (measured weight ≥stated weight). The prevalence of hemorrhagic transformation on follow-up, determined by gradient-recalled echo MRI or non-enhanced CT, was compared between the 2 groups. The predictors for hemorrhage with progression, defined as an increase in the National Institutes of Health Stroke Scale (NIHSS) by a value of 4 or more accompanied by hemorrhage, were determined using multivariable logistic regression analysis and included the overused or underused alteplase and baseline clinical and laboratory findings. <B><I>Results:</I></B> Sixty-six (51.6%) of 128 patients were in the underused group and 62 patients (48.4%) in the overused group. The median difference between the stated and measured weights was 1.5 (interquartile range 0.56-3.81) kg, with the largest difference being 25.6 kg. Although there were no significant difference in baseline clinical and laboratory findings between the 2 groups, the overused group showed a significantly higher prevalence of hemorrhagic transformation (<I>p</I> = 0.012) and hemorrhage with progression (<I>p</I> = 0.025). The multivariable logistic regression analysis demonstrated that overused alteplase (OR 7.26; 95% CI 1.24-42.45; <I>p</I> = 0.028), baseline glucose (>144 mg/dL; OR 5.03; 95% CI 1.00-25.26; <I>p</I> = 0.050), and initial NIHSS (OR 1.13 per 1-point NIHSS increase; 95% CI 1.00-1.27; <I>p</I> = 0.047) in model 1 that use alteplase overdose as a categorical variable and overused alteplase (OR 1.67 1-mg increase; 95% CI 1.05-2.66; <I>p</I> = 0.027) in model 2 that use an overused alteplase dose as numerical variable were significant predictors for hemorrhage with progression. <B><I>Conclusion:</I></B> More alteplase usage than actual weight led to higher hemorrhagic transformation. As one of the predictors for clinical deterioration, it is important to administrate alteplase based on an accurately measured weight.</P>

지속적 경막외마취에 의한 전자간증 환자의 제왕절개술

신동엽,김성주,문순홍,박장수,홍기혁 대한마취과학회 1996 Korean Journal of Anesthesiology Vol.30 No.4

Background: Adequate evaluation and monitoring for pre-eclamptic paturient and capable assistance before induction for anesthesiologist, must be taken to avoid sudden severe maternal hypertension with intubation during a Rapid Sequence intravenous induction. Such event predispose the paturient to intracranial hemorrhage and pulmonary hypertension with pulmonary edema. To diminish danger of hypertension that can be developed during general anesthesia and facilitate control of blood pressure, 24 women presenting for cesarean section were studied. Methods: All received 17.9±2.6ml of 0.5% bupivacaine, including 3ml of test dose, through the epidural catheter inserted in T-L interspace using 18 gauge Tuohy needle to the patients with a lateral decubitus position. We measured blood pressure and heart rate in 5, 10, 15, 20, 30, 45, and 60 minutes after injection of 0.5% bupivacaine and 15 minutes after transferred to recovery room. Results: The blood pressure of the patients started to decrease in 5 minutes and mot decreased in 20 minutes after injection, the heart rate had little change but decreased significantly in 45 minutes. Apgar Scores of the neonates at 1 and 5 minutes were 7.3±2.0 and 9.2±1.5. The patients used ephedrine and crystalloid solution for correction of hypotension were 6 of 24 women and had no any systemic toxicity or neurologic symptoms by local anesthetics(bupivacaine). Conclusion: Continuous epidural anesthesia for cesarean section in preeclamptic patients is recommended for safe anesthesia.

Investigation on the Safety of Boeun U Ore Using Multiple Environmental Tracers

YeoJin Ju,Min Hoon Baik,Seung Yeop Lee,Kang-Kun Lee,Dugin Kaown,Dongbok Shin,Ji-Hun Ryu 한국방사성폐기물학회 2023 한국방사성폐기물학회 학술논문요약집 Vol.21 No.1

Uranium inventory in Boeun aquifer is facing the artificial reservoir that intended for supplying water to nearby cities (40-70 m apart) where, toxic radionuclides might mobile and enter the reservoir. In order to understand U mobility in the system, groundwater and fracture filling materials (FFMs) were analyzed for microbial signatures, C, O, Fe, S and U-series isotopes. The δ18O-H2O and 14C signatures suggested groundwater was originated from upland recharges dominantly and not affected by mixing with the surface water. However, the 234U/238U activity ratios (ARs) and 230Th/234U ARs in FFMs ranged from 0.93 to 1.67 and from 0.22 to 1.97, respectively, indicating that U was mobile along the fractures. In shallow FFMs, the U accumulations (~157 mg/kg) were found with Fe enrichments (~226798 mg/kg) and anomalies of δ56Fe and δ57Fe, implied U mobility in shallow depths was associated with Fe-rich environment. Also, in the shallow depths, Fe-oxidizers, Gallionella was prevailing in groundwater while Acidovorax was abundant near U ore depth. The Fe-rich environment in shallow depths was formed by pyrite dissolution, demonstrated using δ34S-SO4 and δ18O-SO4 distribution. Conclusively, the Fe-rich aquifer was capable of immobilizing the dissolved U through biotic and abiotic processes, without significant discharge into the nearby reservoir.

The study of physical mixing effect to improve the activity of NiCo-based OER elctrocatalyst

Eon-ju Park,Sungjun Heo,Chiho Kim,Song Jin,Jae-Yeop Jeong,Shin-woo Myeong,Ho-seok Lee,Jae-hun Lee,Nam-in Kim,Jun Young park,Jooyoung Lee,Oi Lun Helena Li,Sung Mook Choi 한국표면공학회 2023 한국표면공학회 학술발표회 초록집 Vol.2023 No.11-1

피부표면에서 경막외강까지의 거리

김성주,김철호,신동엽,문순홍,홍기혁 인제대학교 1995 仁濟醫學 Vol.16 No.3

1994년 3월부터 1994년 8월까지 인제대학교 상계백병원 마취과에서 경막외 마취 및 통증 완화를 목적으로 경막외 카테터 삽입을 시행한 589명을 대상으로 하여 피부에서 경막외강까지의 거리를 측정함으로써 경막외강에 카테터를 정확하게 거치시키는데 도움을 주고자 문헌 고찰과 함께 보고하고자 한다. Lumbar epidural analgesia is a well established method for providing postoperative pain relief. Placement of a needle in the epidural space is probably one of the two technically demanding procedures performing by anesthesiologists. Authors studied 589 patients who received epidural analgesia for postoperative pain control. The distance to the epidural space from skin surface was measured by 18G Tuohy needle with marks at each 1cm from the tip. The mean distance was appeared to be 3.92cm, which was correlated with other anatomic measurements, but it was varied with the lumbar inter-space measured. Most epidural spaces were identified at 3 to 5cm and 92.4% of the cases were less than 5cm. This study suggests that if the epidural space has not been identified within a depth of 6cm, redirection or re-insertion of the needle should be considered.