오징어 내장을 쏘가리 사료로서 활용하기 위한 기초연구 1. 오징어 내장의 주요 성분 특성

전중균,주동식,조순영,김양배 강릉대학교동해안지역연구소 1998 東海岸硏究 Vol.8 No.2

To obtain high quality fish oil and feedstuff from by-product of squid processing, especially squid viscera, the raw materials should be freshed and have low lipid oxidation. In addition, the lipid content in stickwater produced from raw squid viscera by centrifuging should be low level. We investigated on processing acceptability for viscera in domestic and exotic squids to prepare a feed for mandarin fish. Lipid oxidation in domestic squid viscera occured less than that in exotic squid one. It assumed that exotic squid was stored at higher temperature condition and for longer time, compared to domestic squid. The free amino acids content in domestic squid viscera was higher than that in exotic squid one. The domestic squid viscera was more acceptable as feed material for mandarin fish than exotic squid one.

일본 수산식품제조업의 원료조달과 수산물시장의 변모

조순영,주동식,전중균 강릉대학교동해안지역연구소 1998 東海岸硏究 Vol.8 No.2

There are many problems for development of fisheries and its processing in our country, and one of them is the marketing of fisheries. Bring to a settlement of this problem, it requires searching of fisheries market and supplying of raw materials for seafood processing in Japan, developed-country in this fields. At first, we research the characteristics of seafood processing and raw material supplying, and demand changing of seafoods and locality of seafood processing in Japan. This researching will provide the direction of development and the method of effective marketing of fisheries and seafood processing in our country.

멸치젓의 呈味成分

李應昊,金世權,錢重均,金洙賢,金程均 釜山水産大學校 1982 釜山水産大學 硏究報告 Vol.22 No.1

멸치젓의 呈味成分에 관한 기초자료를 얻고자 생멸치와 이를 原料로 하여 제조된 멸치젓 중의 核酸關聯物質, 유리아미노산, betaine, TMAO, TMA 및 總creatinine을 分析하였다. 核酸關聯物質 중 생멸치에서는 IMP가 乾物量基準으로 2.8 μmole/g로 가장 많았으나 멸치젓에서는 hypoxanthine이 4.2 μmole/g으로 가장 많았고 , ATP는 흔적량에 불과 하였다. 멸치젓 중의 유리아미노산 중 함량이 많은 것은 leucine, isoleucine, phenylalanine, lysine, tyrosine, alanine, histidine, valine, methionine의 順이었고 glycine, proline, glutamic acid, arginine, threonine, serine 등은 함량이 적었다. 그리고 全유리아미노산은 젓갈 숙성중생원료에 비하여 약 2배로 增加 하였다. Betaine 은 생멸치 및 멸치젓 중에 各各 10.2㎎/100g, 30.0㎎/100/g였으며 이들이 엑스分室素에 대한 比率은 各各 0.6% 및 1.2%였다. TMAO 含量은 생멸치가 13.9㎎/100g, 멸치젓이 9.0㎎/100g였으며 TMA 含量은 各各 22.7㎎/100g 및 30.9㎎/100g였다. 總 creatinine 含量은 생멸치 및 멸치젓에서 各各 433.1㎎/100g, 575.8㎎/100g로서 엑스分室素에 대해 27.2% 및 22.8%였다. Omission test 結果 멸치젓의 呈味成分으로서는 유리아미노산 및 核酸關聯物質이 중요한 구실을 한다는 것을 알았다. This study was conducted to evaluate the taste compounds of fermented anchovy. The contents of such compounds as nucleotides and their related compounds, free amino acids, betaine, TMAO and total creatinine raw and fermented anchovy were analysed. The contents of IMP in raw anchovy appeared higher than other nucleotides and tended to decrease rapidly during fermentation. Therefore, in the case of fermented anchovy, hypoxanthine was abundant and IMP was very low in content. In the free amino acid composition of fresh samples abundant amino acids were leucine, isoleucine, phenylalanine, lysine, tyrosine, alanine, histidine, valine, methionine in order. Such amino acids as glycine, proline, glutamic acid, arginine, threonine and serine were poor in content. The contents of total free amino acids reached approximately about two times as compared with that of raw sample. The amount of betaine and TMA increased, while TMAO decreased during fermentation. The content of total creatinine in raw and fermented anchovy was occupied 22.8% and 27.2% of total of extractive nitrogen, respectively. As the results of omission test, the taste-active compounds of fermented anchovy are assumed to be free amino acids and nucleotides.

까치복의 呈味成分

李應昊,鄭秀烈,趙舜榮,錢重均,車庸準 釜山水産大學校 1983 釜山水産大學 硏究報告 Vol.23 No.1

까치복의 呈味成分에 관한 資料를 얻고자 核酸關聯物質, 유리아미노산, TMAO, TMA, betaine 및 총 creatinine을 分析하였다. 核酸關聯物質 중 IMP가 1.9μmole/g으로서 가장 많았고, 全核酸關聯物質에 대해 39.6%를 차지하였다. 유리아미노산 중 함량이 많은 것은 taurine, lysine, alanine 및 glycine 이고, 이들 네 가지 아미노산이 전유리아미노산의 68.2%를 차지하였다. 총 creatinine�량은 194.5mg/100g으로서 엑스分窒素에 대해 63.4%를 차지하였으며, TMAO 및 betaine 함량은 微量이었다. Omission test 結果 까치복의 呈味成分은 5'-mononucleotides 및 유리아미노산이 주된 구실을 한다는 것을 알았다. Yellowfin puffer, Fugu xanthopterus(Temminck et Schlegel), is one of the most palatable fishes in Korea. However, relatively little is known about the characteristics of its taste. In this study, the taste compounds including nucleotides and their related compounds, free amino acids, trimethylamine oxide, trimethylamine, betaine and total creatinine were analyzed, and their roles in tasting activity were investigated. The amounts of IMP was 1.9 μmole/g, and the ratio of IMP to the total nucleotides and their related compounds was 39.6%. The great portion of free amino acids in the extractives of yellowfin puffer muscle was occupied by taurine, lysine, alanine and glycine in order, and their content was 79.1% of the total free amino acids. Among the organic bases, total creatinine was abundant, and its nitrogen content was 63.4% of total extractive nitrogen. According to the results of the omission test, the main constituents of the characteristic taste of yellowfin puffer could be assumed as free amino acids and 5'-mononucleotides.

유기주석화합물에 단기간 노출시킨 넙치 간장 약물대사효소의 in vivo 및 in vitro 반응

전중균 ( Joong Kyun Jeon ),이지선 ( Ji Seon Lee ),전미정 ( Mi Jeong Jeon ),심원준 ( Won Joon Shim ),임한규 ( Han Kyu Lim ) 한국환경생물학회 2004 환경생물 : 환경생물학회지 Vol.22 No.1

유기주석화합물인 tributyltin chloride (TBTC)와 tributyltin chloride (TPTC)를 넙치 간장으로 만든 미크로좀에 in vitro적으로 노출시켜서 이들 화합물의 대사에 관여하는 mixed function oxidase (MFO) 중 cytochrome P450 (CYP) 농도와 7-etho-xyresorufin deethylase (EROD) 활성의 변화를 조사하였으며, 또한 in vitro 실험에서는 TPTC를 넙치에게 복강주사(7.5 mg kg^(-1) BW)하여 간장의 MFO (CYP농도, NADPH cytochrome c 환원효소 활성, NADH cytochrome b5 환원 효소 활성, EROD 활성) 반응을 경시적으로 조사하였다. 그 결과, in vitro에서는 TBTC, TBTO및 TPTC가 모두 CYP 농도와 EROD 활성을 저해하였으며, 저해력은 TPTC가 가장 컸고 이어서 TBTO, TBTC의 순이었다. 유기주석화합물의 노출농도와 노출시간과 비례하면서 저해 정도가 커졌으며, 특히 EROD활성의 저해는 노출농도에 크게 의존적이었다. 그리고 in vivo 실험에서도 유기주석화합물은 CYP농도, NADPH cytochrome c환원효소 활성, NADH cytochrome b5 환원효소 활성, EROD 활성을 억제하였다. EROD활성은 오염물질에 의한 반응이 민감하고 재현성도 있어 바람직한 측정지표로 이용될 수가 있을 것이다. Cytochrome P450 (CYP) contents and 7-ethoxyresorufin-O-deethylase (EROD) activity were determined in hepatic microsome of olive flounder (Paralichthys olivaceus) exposed to tributyltin chloride (TBTC), tributyltin oxide (TBTO), and triphenyltin chloride (TPTC). In addition, effects of in vivo (intraperitoneal injection of 7.5 mg kg^(-1) BW) exposure of flounder to TPTC on CYP, NADPH cytochrome c reductase, NADH cytochrome b5 reductase and EROD levels were measured. In in vitro exposure of hepatic microsome to organotins, TBTC, TBTO and TPTC reduced CYP contents and inhibited EROD activity. The TPTC was the strongest inhibitor, which is followed by TBTO and TBTC. The degree of inhibition, especially EROD acitivity, depended on the exposure duration. In addition, all the target enzymes in flounder were inhibited by TPTC with the in vivo exposure to TPTC. As EROD activity was the most sensitive to the inhibitions and demonstrated good reproducibility of the results, it could be used as a helpful tool for monitor effects of organotin compounds on mixed funciton oxygenase system in marine fish.

말쥐치 스테이크加工條件 및 凍結貯藏中의 品質安定性

錢重均(Joong-Kyun Jeon),鄭秀烈(Soo-Yeol Jung),河在浩(Jae-Ho Ha),朴香淑(Hyang-Suk Park),李應昊(Eung-Ho Lee) 한국식품과학회 1984 한국식품과학회지 Vol.16 No.2

노닐페놀을 주사한 조피볼락의 간장 cytochrome P450과 EROD의 반응

전중균 ( Joong Kyun Jeon ),이지선 ( Ji Seon Lee ),손영창 ( Young Chang Sohn ),심원준 ( Won Joon Shim ),정지현 ( Jee Hyun Jeung ),홍경표 ( Gyong Pyo Hong ),김병기 ( Pyong Kih Kim ),한창희 ( Chang Hee Han ) 한국환경생물학회 2004 환경생물 : 환경생물학회지 Vol.22 No.1

비이온성 계면활성제로 많이 쓰이는 알킬페놀류의 하나인 노닐페놀(nonylphenol)이 해산어류에게 미치는 영향을 조사하려고 주요 양식어종인 조피볼락에게 복강주사로 10 및 25 mg kg^(-1)을 1회 투여하였다. 한편 용제인 DMSO만을 주사한 sham구를 설정하여 비교하였다. 주사 후 7일간 간중량지수(hepatosomatic index)의 변화를 조사하였고, 또한 간장 미크로좀 중 대표적인 약물대사효소인 cytochrome P450 (CYP) 농도와 ethoxyresorufin deethylase (EROD) 활성의 변화도 측정하였다. 그 결과, 고농도(25 mg kg^(-1)) 주사구에서는 HSI가 감소 하였는데,이것은 NP가 간장에 독성이 있다는 것을 보여준다. 한편, 약물대사효소 중 CYP농도는 유도되었지만 EROD활성은 저해되는 경향을 보였다. 이번에 주사한 농도는 우리나라 연근해 해수 중의 NP농도에 비해 매우 높은 수준이지만,본 실험에서는 NP가 조피볼락에게 간장 독성을 보였고, 간장의 약물대사효소에도 영향을 미친다는 것을 확인할 수 있었다. Nonyphenol (NP) used actively as non-ionic surfactant is classified as one of most potent endocrine disrupting chemicals. Effects of NP on mixed function oxygenase (MFO) system in rockfish (Sebastes schlegeli) were investigated for seven days after intraperitoneal injection (10 and 25 mg kg^(-1)). Hepatosomatic index (HSI) of fishes exposed to NP of 25 mg kg^(-1) was significantly reduced compared to those in control group. NP exposure enhanced cytochrome P450 levels in the fish liver, while 7-ethoxyresorufin-O-deethylase (EROD) activity was inhibited. NP exposure levels in this study were much higher than those found in the coastal environment of Korea. Effects on HSI and liver MFO system, which is involved in steroid hormone metabolism, imply that NP may influence on reproduction of fish by not only hormone receptor mediated response but also through effects on the MFO system.

Randomized clinical trial to evaluate the efficacy and safety of two types of sandblasted with large-grit and acid-etched surface implants with different surface roughness

Jeon Jun-Hyung,Kim Min-Joong,윤필영,Jo Deuk-Won,Kim Young-Kyun 대한구강악안면외과학회 2022 대한구강악안면외과학회지 Vol.48 No.4

Objectives: This study aims to evaluate the efficacy and safety of two types of sandblasted with large-grit and acid-etched (SLA) surface implants with different surface roughness. Patients and Methods: This study was conducted based on a clinical record review of 55 patients (mean age, 53.00 years). A total of 80 SLA sur-face implants was placed. Among the 80 implants, 38 implants placed in 29 subjects had surface roughness (Ra) of 3.09 µm (test group, TG), while the other 42 implants placed in 31 subjects had a surface roughness (Ra) of 2.50 µm (control group, CG). A comparison was made of implant primary/secondary stability; success and survival rates; marginal bone loss; and soft tissue assessment including probing pocket depth (PPD), plaque index (PI), gingival index (GI), and bleeding on probing (BOP) between the groups at 1 year after implant placement. Results: Among the implants that were initially registered, 1 from the TG and 4 from the CG dropped out, leaving 37 implants in the TG and 38 im-plants in the CG to be traced and analyzed. Although 1 TG case showed unstable primary stability, all cases showed stable secondary stability. Success and survival rates at 1 year after implant placement were 100% in both groups. Marginal bone loss was 0.07 mm and 0.00 mm for the TG and CG, respectively, but the difference was not significant. Among the several parameters for evaluation of soft tissue, the TG showed lower PI at 1 year after implant placement (TG=0.00, CG=0.29; P=0.0004), while the remaining categories showed no significant difference between the groups. Conclusion: This study shows that the two types of SLA implants with different surface roughness have no difference in efficacy or safety. Therefore, both of the implants can be used safely and with promising outcomes.

어류 혈액 성분의 저장 안정성

전중균,허형택,김병기 한국수산학회 1995 한국수산과학회지 Vol.28 No.2

조피볼락, 잉어 및 무지개송어의 혈청을 상온(15℃), 저온(4℃), 냉동(-20℃)으로 보존하면서 총단백질(TP), 알부민(ALB), 중성지방(TG), 콜레스테를(CHOL), 포도당(GLC), 인(P), 나트륨(Na), AST 및 ALT의 활성 변화를 상온과 저온에서는 16일까지, 냉동에서는 30일까지 조사하였다. 어종간에 다소의 차이가 있기는 하지만 대체로 단백질 성분인 TP, ALB과 당 성분인 GLC, 무기질인 P, Na의 농도는 안정하였고, 지방 성분인 TG와 CHOL, AST, ALT의 활성은 불안정하였다. 그리고 온도에 따른 혈액 성분의 안정성은 상온에서 보다는 저온과 냉동에서 대체로 안정하지만, 성분에 따라서는 냉동하여도 매우 불안정한 것이 있다. An attempt was made to elucidate the stability of serum metabolites and enzyme activities in blood samples taken from rockfish(Sebastes schlegeli), Israeli carp(Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss) under different storing conditions. The concentrations of total protein(TP), albumin(ALB), triglyceride(TG), cholesterol(CHOL), glucose (GLC), phosphorus(P) and sodium(Na), and the activities of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in serum were investigated for 16 days at 15℃(room temp.) and 4℃ (refrigerative) condition, or frozen at -20℃ for period of 30 days. Though there was a little difference between fish species, the concentrations of TP, ALB, GLC, P, Na in serum were stable at all storing temperatures, while those of TG, CHOL, ALT and AST were not stable, particularly even at the normal temperature. In general, serum components were more stable at refrigerative and frozen conditions than at room temp. storing. However, it was noticeable that the stability of CHOL in rockOsh serum was found to be more unstable at -20℃ than kept at 15℃ or 4℃.

Liquid Chromatography-Tandem Mass Spectrometry of a New PPARα/γ Dual Agonist PAR-5359 in Rat Plasma

Dong Kyun Kim,Eun Jeong Park,Ji Hyun Jeong,Sun Ho Jeon,Eun Jung Kim,Hyun Joo Shim,Joong In Lim,Hye Suk Lee 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.12

PAR-5359, 3-(4-{2-[4-(4-Chloro-phenyl)-3,6-dihydro-2H-pyridin-1-yl]-ethoxy}-phenyl)-2-ethoxypropionic acid, is a well-balanced PPARα/γ dual agonist with the excellent antihyperglycemic and hypolipidemic activities. A reliable, selective and sensitive high-performance liquid chromatography with electrospray ionization tandem mass spectrometry was developed for the determination of PAR-5359 in rat plasma. PAR-5359 was twice extracted from rat plasma using methyl tert-butyl ether at neutral pH. The analytes were separated on an Allure Biphenyl column with the mobile phase of 78% methanol in 10 mM ammonium formate (pH 3.0) and detected by tandem mass spectrometry in the selective reaction monitoring mode. The calibration curve was linear (r = 0.9993) over the concentration range of 2.00-1000.0 ng/mL and the lower limit of quantification was 2.00 ng/mL using 50 μL plasma sample. The coefficient of variation and relative error at four QC levels were 1.2 to 12.3% and -2.5 to 6.3%, respectively. The present method was successfully applied to the pharmacokinetic study of PAR-5359 after oral dose of PAR-5359 at a dose of 1 mg/kg to male SD rats.