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( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.
Discordance in Spirometric Interpretations Based on Korean and Non-Korean Reference Equations
Nami Kim,Mi-Hee Park,Se-yeong Kim,Chunhui Suh,Sangyoon Lee,Kun-Hyung Kim,Chae-Kwan Lee,Dae-Hwan Kim,Jong-Tae Lee 대한직업환경의학회 2013 대한직업환경의학회지 Vol.25 No.-
Objectives: Korean regression models for spirometric reference values are different from those of other ethnic groups. The purpose of this study was to evaluate discordance in spirometric interpretations based on using Korean reference equations versus non-Korean reference equations. Methods: Spirometry was performed on 825 Korean male workers from April 2009 to November 2011. The spirometric patterns and disease severity were evaluated using two Korean equations (Choi’s and Lee’s) and three equations for Caucasians (NHANES III, Morris’s, and Knudson’s), and the results of Choi’s equation were compared with the non-Korean equations. The spirometric patterns were defined as normal, restrictive, and mild and moderate obstructive. Results: The mean differences in the FEV1% and FVC% between the two Korean equations were 2.0 ± 1.3% and 3.5 ± 2.2%, respectively. Morris’s equation had the greatest difference in the FEV1% from Choi’s equation: 32.9 ± 8.5%. Knudson’s equation had the greatest difference in the FVC% from Choi’s equation: 10.5 ± 6.5%. Conclusions: The two Korean equations shared similar characteristics of spirometric interpretation. The spirometric interpretations of Choi’s equation were significantly different from those of Morris’s equation and Knudson’s equation.
Cytotoxic and Antitumor constituens from Korean medicinal plants
Bae, Ki-Hwan,Jung, Hyun-Ju,Park, Jin-Young,Na, Min-Kyun,Lee, Jong-Pill 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1
KiHwan BAE, HyunJu Jung, Jin Young Park, MinKyun Na, and JongPill Lee*. College of Pharmacy, Chungnam National University, Taejon and *Natural Medicinal Products Division, Korea Food & Drug Administration, Seoul, Korea. Cytotoxic and antitumor constituens from Korean medicinal plants. Preceedings of International Symposium on East-West Medicine, Seoul. Page 153-161, 1999. -For the screening of bioactive natural products, the benzene or methanol extracts from 93 medicinal plants of Korea were prepared, and tested for the cytotoxicity against L1210 cells and for the antitumor action (Bae et al., 1992 and 1996). Of 93 extracts tested, 6 samples showed a cytotoxicity in both benzene and methanol extract, 39 samples in benzene and 13 samples in methanol extract. The benzene extract of the root of scutellaria indica L., Carpesium abrotanoides L., Gymnaster koraiensis(Nakai) Kitamura, Pyrola japonica Klenze, Forsythiae Fructus, and Alismatis Rhizoma showed a potent cytotoxic activity. This observation led to isolate active cytotoxic components, some of which demonstrated some antitumor action. In addition, the structure-activity relationship was discussed.
Breeding of New Ever-bearing Strawberry “Doha” Variety
Jong Nam Lee(Jong Nam Lee),Jong Taek Suh(Jong Taek Suh),Su Jeong Kim(Su Jeong Kim),Hwang Bae Sohn(Hwang Bae Sohn),Do Yeon Kim(Do Yeon Kim),Jung Hwan Nam(Jung Hwan Nam) 한국자원식물학회 2022 한국자원식물학회지 Vol.35 No.6
“Doha” is a new strawberry (Fragaria x ananassa Duch.) variety, which was released by the Highland Agriculture Research Institute in 2021. The “Doha” variety originates from a 2014 cross between “Saebong No. 3” and “Yeolha,” both of which exhibited excellent ever-bearing characteristics, including continuous flowering and large fruits under long-day and high temperature conditions. This new cultivar was initially named “Saebong No. 13” after examining its characteristics and productivity during summer cultivation between 2015 and 2018. After regional adaptability tests, “Doha” was selected from “Saebong No. 13” as an elite cultivar. The general characteristics of “Doha” include spreading, elliptic leaves, and strong growth. The fruits are long and conical and of a red color. The plant height of “Doha” was similar to that of “Goha,” but the number of leaves was lower. The number of flower clusters of “Doha” was 8.6, which was 2.8 fewer than that of the control variety, “Goha,” with 11.4. The average fruit weight of “Doha” was 13.9 g, which was 4.9 g heavier than that of “Goha.” The fruit hardness of “Doha” was 35.5 g·㎜−2, which was 9.4 g·㎜−2 harder than that of “Goha.” The marketable yield of “Doha” was 26,971 ㎏·㏊−1, 125% more than that of “Goha” with 21,479 ㎏·㏊−1. The findings of this study suggest that “Doha” is a hard fruit and high-yielding variety of ever-bearing strawberries that could increase farming income when distributing to farmers.
H II REGION LUMINOSITY FUNCTION OF THE INTERACTING GALAXY M51
Lee, Jong Hwan,Hwang, Narae,Lee, Myung Gyoon IOP Publishing 2011 The Astrophysical journal Vol.735 No.2
<P>We present a study of H II regions in M51 using the Hubble Space Telescope Advanced Camera for Surveys images taken as part of the Hubble Heritage Program. We have cataloged about 19,600 H II regions in M51 with H alpha luminosity in the range of L = 10(35.5)-10(39.0) erg s(-1). The H alpha luminosity function of H II regions (H II LF) in M51 is well represented by a double power law with its index alpha = -2.25 +/- 0.02 for the bright part and alpha = -1.42 +/- 0.01 for the faint part, separated at a break point L = 10(37.1) erg s(-1). This break was not found in previous studies of M51 H II regions. Comparison with simulated H II LFs suggests that this break is caused by the transition of H II region ionizing sources, from low-mass clusters (with similar to 10(3) M-circle dot, including several OB stars) to more massive clusters (including several tens of OB stars). The H II LFs with L < 10(37.1) erg s(-1) are found to have different slopes for different parts in M51: the H II LF for the interarm region is steeper than those for the arm and the nuclear regions. This observed difference in H II LFs can be explained by evolutionary effects: H II regions in the interarm region are relatively older than those in the other parts of M51.</P>
Lee, Jin Hwan,Lee, Byong Won,Kim, Balo,Kim, Hyun Tae,Ko, Jong Min,Baek, In-Yeoul,Seo, Weon Taek,Kang, Young Min,Cho, Kye Man The Korean Society for Applied Biological Chemistr 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.6
Contents of phenolic compound including isoflavones and phenolic acids as well as antioxidant effects in high-protein soybean cultivar "Saedanbaek" were evaluated under different roasting conditions. The roasted soybean exhibited significantly higher antioxidant activity than unroasted soybean in the three antioxidant methods including 2,2-diphenyl-1-picrylhydrazyl, 2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid), and Ferric reducing antioxidant power. In particular, the roasted soybean at $200^{\circ}C$ for 15 min showed the highest antioxidant activity in comparison with other conditions. The contents of phenolic compounds, isoflavone aglycones (genistein, daidzein, and glycitein), isoflavone ${\beta}$-glucosides (genistin, daidzin, and glycitin), and phenolic acids increased, whereas isoflavone malonyl-${\beta}$-glucosides decreased during roasting process. Moreover, total phenolic and flavonoid contents as well as those of isoflavone aglycones, isoflavone-${\beta}$-glucosides, and phenolic acids increased, leading to a general increase in antioxidant activity after roasting. These results suggest that the roasting soybean extracts could contribute to obtaining natural antioxidants in certain food applications.
Lee, Ki-Jong,Yi, Bu-Young,Kim, Kyung-Hwan,Kim, Jung-Bong,Suh, Seok-Cheol,Woo, Hee-Jong,Shin, Kong-Sik,Kweon, Soon-Jong The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.1
Increasing vitamin E activity in economically important oil crops such as soybean will enhance the nutritional value of these crops. An improved soybean transformation system involving pre-culture of soybean seed on medium supplemented with 1 mg/L 6-benzylaminopurine under dark conditions was established. To improve the nutritional value of soybean by increasing the ${\alpha}$-tocopherol content, soybeans were transformed with ${\gamma}$-tocopherol methyltransferase (${\gamma}$-TMT) gene by Agrobacterium-mediated transformation. Frequency of soybean transformation was significantly increased from 0.5 to 4.3% by this modified system, and 13 lines of transgenic soybean plants containing ${\gamma}$-TMT were obtained. The molecular characterization using polymerase chain reaction (PCR), reverse transcriptase PCR, and Southern blot analysis confirmed insertion and inheritance of the transgene in the transgenic plants and their progeny. Morphologically normal and fertile transgenic plants were analyzed, and the majority of transgenic soybean transmitted herbicide resistance at 3:1 or 15:1 ratios to their progeny. Alpha-tocopherol content in transgenic soybean seeds was determined by high-performance liquid chromatography; over-expression of ${\gamma}$-TMT resulted in a 41-fold increase in ${\alpha}$-tocopherol over wild-type soybean seeds.