Effect of Macromolecules in Maturation Medium on Oocyte Maturation and Embryonic Development after Parthenogenesis and Nuclear Transfer in Pigs

Jinyoung You,이은송,김진영 사단법인 한국동물생명공학회 2009 한국동물생명공학회지 Vol.24 No.2

The objective of this study was to examine the effect of macromolecule in a maturation medium on nuclear maturation, intracellular glutathione (GSH) level of oocytes, and embryonic development after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) in pigs. Immature pig oocytes were cultured in maturation medium that was supplemented with each polyvinyl alcohol (PVA), pig follicular fluid (pFF) or newborn calf serum (NBCS) during the first 22 h and the second 22 h. Oocyte maturation was not influenced by the source of macromolecules during in vitro maturation (IVM). Embryo cleavage and cell number in blastocyst after PA was altered by the source of macromolecule but no difference was observed in blastocyst formation among treatments. Oocytes matured in PVA-PVA medium showed lower rates of oocyte-cell fusion (70.4% vs. 77~82%) and embryo cleavage (75% vs. 86~90%) after SCNT than those matured in other media but blastocyst formation was not altered (13~27%) by different macromolecules. pFF added to IVM medium significantly increased the intracellular GSH level of oocytes compared to PVA and NBCS, particularly when pFF was supplemented during the first 22 h of IVM. Our results demonstrate that source of macromolecule in IVM medium influences developmental competence of oocytes after PA and SCNT, and that pFF supplementation during the early period (first 22 h) of IVM increases intracellular GSH level of oocytes.

MG132 treatment during oocyte maturation improves embryonic development after somatic cell nuclear transfer and alters oocyte and embryo transcript abundance in pigs

You, Jinyoung,Kim, Jinyoung,Lee, Heowwon,Hyun, Sang‐,Hwan,Hansen, Peter J.,Lee, Eunsong Wiley Subscription Services, Inc., A Wiley Company 2012 Molecular reproduction and development Vol.79 No.1

<P><B>Abstract</B></P><P>The objective of this study was to examine the effect of treating pig oocytes during in vitro maturation (IVM) with a proteasome inhibitor, MG132, on oocyte maturation and embryonic development. In one series of experiments, oocytes from medium‐sized follicles (3–8 mm in diameter) were untreated (MCO) or treated with MG132 during 0–22 hr (M0–22) or 30–42 hr (M30–42) of IVM. There was no significant effect of MG132 on nuclear maturation or cytoplasmic maturation (as assessed by intracellular amounts of glutathione and p34cdc2 kinase activity). Blastocyst formation after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT), however, was increased for M30–42 (65.2% and 27.7% for PA and SCNT, respectively) compared to MCO (42.6% and 13.6%, respectively) and M0–22 (45.3% and 19.5%, respectively; <I>P</I> < 0.05). Expression of PCNA and ERK2 was increased in M30–42 for IVM oocytes while transcript abundance for POUF51, DNMT1, FGFR2, and PCNA was increased in M30–42 for 4‐cell SCNT embryos. When oocytes derived from small follicles (<3 mm in diameter) were untreated (SCO) or treated with MG132 during 0–22 hr (S0–22), 30–42 hr (S30–42) of IVM, or 0–22 and 30–42 hr of IVM (S0–22/30–42), expression of POU5F1, DNMT1, FGFR2, and PCNA and blastocyst formation were increased for SCNT embryos derived from S30 to 42 (16.5%) and S0–22/30–42 oocytes (20.8%) as compared to embryos from SCO (8.7%) or S0–22 oocytes (8.8%; <I>P</I> < 0.05). Results demonstrate that treatment of oocytes with MG132 during the later stage of IVM improves embryonic development and alters gene expression in pigs. Mol. Reprod. Dev. 79:41–50, 2012. © 2011 Wiley Periodicals, Inc.</P>

Post-fusion treatment with MG132 increases transcription factor expression in somatic cell nuclear transfer embryos in pigs

You, Jinyoung,Lee, Joohyeong,Kim, Jinyoung,Park, Junhong,Lee, Eunsong Wiley Subscription Services, Inc., A Wiley Company 2010 Molecular reproduction and development Vol.77 No.2

<P>The objective of this study was to examine the effect of post-fusion treatment of somatic cell nuclear transfer (SCNT) oocytes with the proteasomal inhibitor MG132 on maturation promoting factor (MPF) activity, nuclear remodeling, embryonic development, and gene expression of cloned pig embryos. Immediately after electrofusion, SCNT oocytes were treated with MG132 and/or caffeine for 2 hr, vanadate for 0.5 hr, or vanadate for 0.5 hr followed by MG132 for 1.5 hr. Of the MG132 concentrations tested (0–5 µM), the 1 µM concentration showed a higher rate of blastocyst formation (25.9%) than 0 (14.2%), 0.5 (16.9%), and 5 µM (16.9%). Post-fusion treatment with MG132, caffeine, and both MG132 and caffeine improved blastocyst formation (22.1%, 21.4%, and 24.4%, respectively), whereas vanadate treatment inhibited blastocyst formation (6.5%) compared to the control (11.1%). When examined 2 hr after fusion and 1 hr after activation, MPF activity remained at a higher (P < 0.05) level in SCNT oocytes that were treated post-fusion with caffeine and/or MG132, but it was decreased by vanadate. The rate of oocytes showing premature chromosome condensation was not altered by MG132 but was decreased by vanadate treatment. In addition, formation of single pronuclei was increased by MG132 compared to control and vanadate treatment. MG132-treated embryos showed increased expression of POU5F1, DPPA2, DPPA3, DPPA5, and NDP52l1 genes compared to control embryos. Our results demonstrate that post-fusion treatment of SCNT oocytes with MG132 prevents MPF degradation and increases expression of transcription factors in SCNT embryos, which are necessary for normal development of SCNT embryos. Mol. Reprod. Dev. 77: 149–157, 2010. © 2009 Wiley-Liss, Inc.</P>

Prolonged interval between fusion and activation impairs embryonic development by inducing chromosome scattering and nuclear aneuploidy in pig somatic cell nuclear transfer

You, Jinyoung,Song, Kilyoung,Lee, Eunsong CSIRO Publishing 2010 Reproduction, fertility, and development Vol.22 No.6

<P> The aim of the present study was to examine the effect of various intervals between electrofusion and activation (FA interval) on the nuclear remodelling and development of somatic cell nuclear transfer (SCNT) embryos in pigs. Reconstructed oocytes were activated at 0 (simultaneous fusion and activation; SFA), 1, 2 and 3 h (delayed activation) after electrofusion; these groups were designated as DA1, DA2 and DA3, respectively. When oocyte nuclear status was examined at 0.5, 1, 2 and 3 h after electrofusion, the incidence of chromosome scattering was increased (P < 0.01) as the FA interval was extended (0.0%, 12.0%, 77.3% and 78.0%, respectively). Extending the FA interval led to an increase (P < 0.01) in the percentage of oocytes containing multiple (≥3) pseudopronuclei (PPN) (0.0% of SFA; 5.3% of DA1; 21.7% of DA2; and 33.5% of DA3). The development of SCNT embryos to the blastocyst stage was decreased (P < 0.05) in DA2 (5.7%) and DA3 (5.0%) compared with SFA (18.1%) and DA1 (19.5%). Our results demonstrate that extending the FA interval impairs the development of SCNT pig embryos by inducing chromosome scattering and the formation of multiple PPN, which may result in increased nuclear aneuploidy. </P>

Reproductive Efficiency and Characteristics of Cloned Miniature Piglets Produced from Domestic Commercial Gilts

Jinyoung You,Yu-Byeol Jeon,현상환,박수봉,이은송 사단법인 한국동물생명공학회 2010 한국동물생명공학회지 Vol.25 No.4

The objective of this study was to examine the reproductive characteristics of cloned miniature piglets produced from surrogate domestic pigs. Somatic cell nuclear transfer (SCNT) miniature pig embryos were transferred into domestic pigs. As controls, domestic pigs of the same breed with surrogates for SCNT embryos and miniature pigs of the same breed with the somatic cell donor were bred by artificial insemination and natural mating, respectively. Surrogate domestic pigs that farrowed cloned miniature piglets had a significantly longer gestation length (118.1 days)than conventionally bred domestic (115.4 days) and miniature (115.5 days) pigs. Furthermore, the birth weight of cloned miniature piglets produced from domestic pigs (743 g) was significantly greater than that of miniature piglets produced by natural breeding (623 g). Also, cloned miniature piglets had a significantly lower weaning rate (49.7%)than conventionally produced domestic (91.5%) and miniature (100%) piglets. No differences were observed between female and male cloned piglets in gestation length, litter size, birth weight, or weaning rate. Our results demonstrate that gestation length is extended in domestic pigs that are transferred with SCNT miniature pig embryos and that cloned miniature piglets have increased birth weight and high pre-weaning mortality.

슬링을 이용한 신경근 훈련이 중년여성의 상지기능장애 회복에 미치는 효과

유진영 ( Jinyoung You ),김은국 ( Eunkuk Kim ) 한국체육대학교 체육과학연구소 2019 스포츠사이언스 Vol.36 No.2

목적: 본 연구는 40∼69세의 중년여성에서 슬링을 이용한 신경근 훈련과 복합운동이 상지통증과 관절가동범위에 미치는 효과를 알아보기 위해 수행되었다. 방법: 연구참여자는 슬링을 이용한 신경근 훈련과 복합운동그룹 (n=6, NM+Complex)과 복합운동 그룹(n=6, Control)으로 구성되었으며, 연구는 4주 동안 진행되었다. NM+Complex그룹은 슬링을 이용해서 신경근 운동을 주 2회, 자세를 5∼8초간 유지하여, 8회씩 3세트 실시하였으며, 스트레칭은 부하 없이 천천히 15회씩 3세트 실시하였고, 저항 운동은 주 1회 15RM의 부하로 15회씩 3세트 실시하였다. Control그룹은 저항 운동을 주 3회 15RM의 부하로 15회씩 3세트 실시하였고, 스트레칭을 부하없이 천천히 15회씩 3세트 실시하였다. 트레이닝 전, 후의 상지 통증은 DASH 설문지로 관절가동 범위는 측각기로 측정하여 비교하였다. 결과: 1. NM+Complex그룹의 양측 견관절가동범위 증가는 굴곡에서 측정시기에 따른 차이가 있었으며(우측:p=.001, 좌측:p=.003), 좌측 견관절에서 외전(p=.030)과 내회전(p=.030)에서 측정시기에 따른 가동범위 증가에 차이가 있었다. 2. Control그룹의 양측 견관절 가동범위의 증가는 굴곡(우측:p=.027, 좌측:p=.005)과 외전(우측:p=.029, 좌측:p=.009)에서 측정시기에 따른 차이가 있었다. 좌측 견관절에서 신전(p=.026), 내회전(p=.028)에서 측정시기에 따른 가동범위 증가에 차이가 있었다. 3. DASH 설문지로 통증의 변화를 확인한 결과 NM+Complex그룹은 측정시기에 따른 차이가 없었으며, Control그룹은 측정시기에 따른 차이가 있었다(p=.043). 그룹 간의 차이는 없었다(p=.235). 결론: 두 그룹 간 견관절 가동범위 증가와 통증의 감소의 차이는 없었다. Control그룹에서 견관절 신전의 측정시기에 따른 관절가동범위의 증가와 통증 감소가 나타나 견관절 신전의 가동범위의 회복과 통증의 감소를 위해서는 복합운동만을 적용하는 것이 더 효과적일 것이다. 그러나 복합운동만을 적용하기 어려운 경우 견관절의 굴곡과 외전, 내회전에서 관절 가동범위 증가를 위해 슬링을 이용한 신경근 훈련을 대체하여 적용한다면 견관절 가동범위 증가를 위해 유용할 것이다. Purpose: The aim of this study was based on age of 40 to 69 women to prove the effects of neuromuscular training and complex training on upper-extremity pain with joint range of motion. Method: We had a NM+Complex group (n=6, age range 50.50±8.76), and a Control group (n=6, age range 59.83±6.43). NM+Complex group executed both neuromuscular training and complex training, but Control group only worked out the complex training. These neuromuscular training and complex training were by sling exercise, twice a week; each time 8 movements with 3 sets, maintain posture for 5 to 8 seconds. Stretching exercise operated without any loads, 15 times slowly with 3 sets. Resistance exercise operated once a week with 15RM load, 15times with 3 sets. Comparison before and after upper-extremity pains by DASH Score, and joint range of motion by goniometer. Result: Firstly, in the NM+Complex group, right and left shoulder joint range of motion had significant difference with time interval for measurement on flexion. In case with only left shoulder joint range of motion increase, it showed the difference with time interval for measurement on external and internal rotation. Secondly, in the Control group, left shoulder range of motion had significant difference with time interval for measurement on flexion and external rotation. If only left shoulder joint range of motion increase, it showed the difference on extensor and internal rotation. Lastly, upper-extremity pain differences by DASH Score showed no big differences on NM+Complex group, but the Control group showed significant difference. Conclusion: The result of this research is, based on 40 to 69 age of women, to release upper-extremity pain and increase joint range of motion, just taking a complex training is not quite efficient than taking both neuromuscular training and complex training.

항공라이다를 활용한 소나무 재선충 분석

유진영(You, Jinyoung) 대한토목학회 2023 대한토목학회 학술대회 Vol.2023 No.10

Developmental competence of morphologically poor oocytes in relation to follicular size and oocyte diameter in the pig

Kim, Jinyoung,You, Jinyoung,Hyun, Sang-Hwan,Lee, Gene,Lim, Jeongmook,Lee, Eunsong Wiley Subscription Services, Inc., A Wiley Company 2010 Molecular reproduction and development Vol.77 No.4

<P>The objective of this study was to assess the practical usefulness of morphologically poor oocytes (MPCOCs) in relation to follicular size and oocyte diameter. Oocytes collected from medium (3–8 mm in diameter) and small (<3 mm) follicles were classified into five categories of morphologically good oocytes (MGCOCs) from medium follicles (MA, control), MPCOCs with larger and smaller diameters from medium follicles (ML and MS, respectively), and those from small follicles (SL and SS, respectively). The oocytes were examined for maturation and developmental competence after parthenogenesis and somatic cell nuclear transfer (SCNT). Nuclear maturation of ML oocytes (91%) was similar to that of control oocytes (94%), but higher than MS (80%), SL (79%), and SS (63%) oocytes. This pattern was also observed in the intracellular glutathione level, p34<SUP>cdc2</SUP> kinase activity, and gene (CDK1, PCNA, and ERK2) expression levels in in vitro-matured oocytes. ML oocytes showed a similar proportion of blastocyst formation (20%) after SCNT to control oocytes (21%). In addition, the use of ML oocytes resulted in a 50% farrowing rate with 1.8% efficiency of piglet production after SCNT embryo transfer, while control oocytes showed a 60% farrowing rate with 2.4% production efficiency. Our results demonstrate that MPCOCs, if appropriately selected, have a comparable ability to MGCOCs in supporting not only in vitro blastocyst formation, but also development to term in vivo after SCNT. These oocytes can be used as a source for in vitro production of embryos with normal in vivo viability in pigs. Mol. Reprod. Dev. 77: 330–339, 2010. © 2009 Wiley-Liss, Inc.</P>

Association of Serum Biomarkers With Pulmonary Involvement of Rheumatoid Arthritis Interstitial Lung Disease: From KORAIL Cohort Baseline Data

( Jinyoung Moon ),( Jeong Seok Lee ),( Young Im Yoon ),( Sung Hae Chang ),( Yeon Ah Lee ),( You-jung Ha ),( Eun Ha Kang ),( Yong-beom Park ),( Hwa Jung Lee ),( Jung-yoon Choi ),( Eun Young Lee ) 대한류마티스학회 2021 대한류마티스학회지 Vol.28 No.4

Objective. The increase in mortality in rheumatoid arthritis (RA) patients with interstitial lung disease (ILD) is well known. However, there are few studies on serum markers that can evaluate acute exacerbation or prognosis in RA-ILD patients. The purpose of this study was to identify the association between biomarkers and lung lesions in patients with RA-ILD. Methods. We analyzed 153 patients with serum samples in a prospective, multicenter cohort of Korean RA-ILD patients. The serum levels of biomarkers, matrix metalloproteinase (MMP-7), surfactant protein-D (SP-D), and Krebs von den Lungen-6 (KL-6) were measured and correlated with forced vital capacity (FVC), diffusing capacity for carbon monoxide (DLCO) and the results of computed tomography (CT). CT results were interpreted semi-quantitatively according to the extent of lung lesions (grade 1, 0%∼25%; grade 2, 26%∼50%; grade 3, 51%∼75%; grade 4, 76%∼100%). Results. MMP-7, SP-D, and KL-6 were negatively correlated with FVC (MMP-7, r=-0.267, p=0.001; SP-D, r=-0.250, p=0.002; KL-6, r=-0.223, p=0.006) and DLCO (MMP-7, r=-0.404, p<0.001; SP-D, r=-0.286, p=0.001; KL-6, r=-0.226, p=0.007). In addition, MMP-7, SP-D, and KL-6 tended to increase with higher grades of lung lesions on CT (MMP-7, p=0.013; SP-D, p<0.001; KL-6, p<0.001). Conclusion. MMP-7, SP-D, and KL-6 can be used to evaluate the functional and anatomical status of lung involvement in the RA-ILD patients. (J Rheum Dis 2021;28:234-241)

[P8-228] In vivo Hepatoprotective Effects of Curcuma Ionga L. Against Oxidative Stress Induced by Ethanol

Jinyoung Kim,Heami Sung,Jeongjin Park,Yanghee You,Yoohyun Lee,Kyungtack Oh,Kyungmi Kim,Woojin Jun 한국식품영양과학회 2009 한국식품영양과학회 학술대회발표집 Vol.2009 No.11