Temporal expression profiling of long noncoding RNA and mRNA in the peripheral blood during porcine development

Gu, Yiren,Zhou, Rui,Jin, Long,Tao, Xuan,Zhong, Zhijun,Yang, Xuemei,Liang, Yan,Yang, Yuekui,Wang, Yan,Chen, Xiaohui,Gong, Jianjun,He, Zhiping,Li, Mingzhou,Lv, Xuebin Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.5

Objective: We investigated the temporal expression profiles of long noncoding RNA (lncRNA) and mRNA in the peripheral blood of pigs during development and identified the lncRNAs that are related to the blood-based immune system. Methods: Peripheral blood samples were obtained from the pigs at 0, 7, 28, and 180 days and 2 years of age. RNA sequencing was performed to survey the lncRNA and mRNA transcriptomes in the samples. Short time-series expression miner (STEM) was used to show temporal expression patterns in the mRNAs and lncRNAs. Gene ontology and Kyoto encyclopedia of genes and genomes analyses were performed to assess the genes' biological relevance. To predict the functions of the identified lncRNAs, we extracted mRNAs that were nearby loci and highly correlated with the lncRNAs. Results: In total of 5,946 lncRNA and 12,354 mRNA transcripts were identified among the samples. STEM showed that most lncRNAs and mRNAs had similar temporal expression patterns during development, indicating the expressional correlation and functional relatedness between them. The five stages were divided into two classes: the suckling period and the late developmental stage. Most genes were expressed at low level during the suckling period, but at higher level during the late stages. Expression of several T-cell-related genes increased continuously during the suckling period, indicating that these genes are crucial for establishing the adaptive immune system in piglets at this stage. Notably, lncRNA TCONS-00086451 may promote blood-based immune system development by upregulating nuclear factor of activated T-cells cytoplasmic 2 expression. Conclusion: This study provides a catalog of porcine peripheral blood-related lncRNAs and mRNAs and reveals the characteristics and temporal expression profiles of these lncRNAs and mRNAs during peripheral blood development from the newborn to adult stages in pigs.

NDRG2 is involved in the oncogenic properties of renal cell carcinoma and its loss is a novel independent poor prognostic factor after nephrectomy.

Liang, Zhe Long,Kang, Kyeongah,Yoon, Sukjoon,Huang, Song Mei,Lim, Jae Sung,Kim, Jin Man,Lim, Jong-Seok,Lee, Hyo Jin Raven Press 2012 Annals of Surgical Oncology Vol.19 No.8

<P>Although NDRG2 is a candidate tumor suppressor, its exact role in renal cell carcinoma (RCC) is not fully understood. We investigated the functional role of NDRG2 and its clinical relevance in RCC tumorigenesis.</P>

Excision repair cross‐complementation group 1 expression predicts response and survival in locally advanced cervical carcinoma patients treated with concurrent chemoradiotherapy

Liang, Zhe Long,Song, Eun‐,Kee,Ko, Young Bok,Lee, Na‐,Ri,Yhim, Ho‐,Young,Noh, Heung‐,Tae,Yun, Hwan Jung,Suh, Kwang Sun,Jo, Deog Yeon,Kim, Samyong,Kim, Jun‐,Sang,Kim, Jin Blackwell Publishing Ltd 2011 Histopathology Vol.59 No.3

The development of training platform for CiADS using cave automatic virtual environment

Jin-Yang Li,Jun-Liang Du,Long Gu,You-Peng Zhang,Xin Sheng,Cong Lin,Yongquan Wang Korean Nuclear Society 2023 Nuclear Engineering and Technology Vol.55 No.7

The project of China initiative Accelerator Driven Subcritical (CiADS) system has been started to construct in southeast China's Guangdong province since 2019, which is expected to be checked and accepted in the year 2025. In order to make the students in University of Chinese Academy of Sciences (UCAS) better understand the main characteristic and the operation condition in the subcritical nuclear facility, the training platform for CiADS has been developed based on the Cave Automatic Virtual Environment (CAVE) in the Institute of Modern Physics Chinese Academy of Sciences (IMPCAS). The CAVE platform is a kind of non-head mounted virtual reality display system, which can provide the immersive experience and the alternative training platform to substitute the dangerous operation experiments with strong radioactivity. In this paper, the CAVE platform for the training scenarios in CiADS system has been presented with real-time simulation feature, where the required devices to generate the auditory and visual senses with the interactive mode have been detailed. Moreover, the three dimensional modeling database has been created for the different operation conditions, which can bring more freedom for the teachers to generate the appropriate training courses for the students. All the user-friendly features will offer a deep realistic impression to the students for the purpose of getting the required knowledge and experience without the large costs in the traditional experimental nuclear reactor.

Ganoderma Lucidum Polysaccharides Target a Fas/Caspase Dependent Pathway to Induce Apoptosis in Human Colon Cancer Cells

Liang, Zengenni,Guo, Yu-Tong,Yi, You-Jin,Wang, Ren-Cai,Hu, Qiu-Long,Xiong, Xing-Yao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.9

Ganoderma lucidum polysaccharides (GLP) extracted from Ganoderma lucidum have been shown to induce cell death in some kinds of cancer cells. This study investigated the cytotoxic and apoptotic effect of GLP on HCT-116 human colon cancer cells and the molecular mechanisms involved. Cell proliferation, cell migration, lactate dehydrogenase (LDH) levels and intracellular free calcium levels ($[Ca^{2+}]i$) were determined by MTT, wound-healing, LDH release and fluorescence assays, respectively. Cell apoptosis was observed by scanning and transmission electron microscopy. For the mechanism studies, caspase-8 activation, and Fas and caspase-3 expression were evaluated. Treatment of HCT-116 cells with various concentrations of GLP (0.625-5 mg/mL) resulted in a significant decrease in cell viability (P< 0.01). This study showed that the antitumor activity of GLP was related to cell migration inhibition, cell morphology changes, intracellular $Ca^{2+}$ elevation and LDH release. Also, increase in the levels of caspase-8 activity was involved in GLP-induced apoptosis. Western blotting indicated that Fas and caspase-3 protein expression was up-regulated after exposure to GLP. This investigation demonstrated for the first time that GLP shows prominent anticancer activities against the HCT-116 human colon cancer cell line through triggering intracellular calcium release and the death receptor pathway.

Growth and Optical Properties of Nanocrystalline/Microcrystalline DiamondMultilayer Films

Jin-Long Luo,Xuan-Tong Ying,Liang-Yao Chen,Pei-Nan Wang 한국물리학회 2005 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.46 No.2

Nanocrystalline/microcrystalline diamond (NCD/MCD) multilayer film with high sp3 bonded carbon concentration and smooth surface has been successfully deposited on silicon substrate. Optimized deposition parameters based on substrate temperature modulation were adopted to grow the NCD/MCD film. The nanocrystallinity, purity and the multilayer structure were characterized by Raman spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM). The smoothness of the film surface was shown by surface profile measurements and further verified by its high optical transmittance from visible to infrared band. Therefore, this newly proposed NCD/MCD multilayer film would have a variety of optical applications.

Inhibition of DNA repair protein RAD51 affects porcine preimplantation embryo development

Jin, Zhe-Long,Shen, Xing-Hui,Shuang, Liang,Kwon, Jeong-woo,Seong, Min-Jeong,Kim, Nam-Hyung BioScientifica 2019 Reproduction Vol.157 No.3

<P>Homologous recombination (HR) plays a critical role in facilitating replication fork progression when the polymerase complex encounters a blocking DNA lesion, and it also serves as the primary mechanism for error-free DNA repair of double-stranded breaks. DNA repair protein RAD51 homolog 1 (RAD51) plays a central role in HR. However, the role of RAD51 during porcine early embryo development is unknown. In the present study, we examined whether RAD51 is involved in the regulation of early embryonic development of porcine parthenotes. We found that inhibition of RAD51 delayed cleavage and ceased development before the blastocyst stage. Disrupting RAD51 activity with RNAi or an inhibitor induces sustained DNA damage, as demonstrated by the formation of distinct γH2AX foci in nuclei of four-cell embryos. Inhibiting RAD51 triggers a DNA damage checkpoint by activating the ataxia telangiectasia mutated (ATM)-p53-p21 pathway. Furthermore, RAD51 inhibition caused apoptosis, reactive oxygen species accumulation, abnormal mitochondrial distribution and decreased pluripotent gene expression in blastocysts. Thus, our results indicate that RAD51 is required for proper porcine parthenogenetic activation (PA) embryo development.</P>

Study of Application of PTFE Fiber in Self-cleaning Filter Media

Jin Long,Min Tang,Yun Liang,Jian Hu 한국섬유공학회 2018 Fibers and polymers Vol.19 No.12

To provide protection against the adverse effects of high concentration dust, self-cleaning filter was applied in the air inlet system of engine and gas turbine. PTFE fiber could have a great application in self-cleaning filter media due to its low surface free energy. In this work, new PTFE/cellulose fiber composite media for self-cleaning filter were prepared by wet-laid method. The effect of PTFE fiber on physical properties and filtration performance were studied. It was found that PTFE fiber was well dispersed in water without bubble formation after proper surfactant was applied. The initial figure of merit increased significantly when percentage of PTFE fiber was higher. For self-cleaning performance, particles were easier to be detached from composite media with higher PTFE fiber percentage, which resulted in lower average pressure drop. It was estimated that in the loading process, energy consumption of media containing 30 % and 60 % PTFE fiber was 32 % and 47 % less than that of pure cellulose media, respectively. PTFE fiber significantly improved the self-cleaning performance due to lower surface free energy of composite media and higher efficiency per unit thickness.

Voxel-encoded descriptor for 3D model retrieval by exploring model’s spatial information

Jin-Yuan Jia,Qian Zhang,Long Zeng,Shuang Liang 대한기계학회 2014 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.28 No.7

Retrieving similar products with a given one has attracted considerable attention. However, products are usually assembled by multiplecomponents, frustrating the previous visual-based retrieval descriptors. We design a voxel-encoded descriptor (VED) by exploring models’spatial information, i.e., boundary data and internal data. This descriptor is computed in three steps. First, the posture of a polygonalmodel is normalized by improved voxel-based principal component analysis technique. Then, six color images are generated by projectingthe voxels along its six local axes. The color value of each pixel encodes status of all voxels intersecting with the ray starting from thepixel and parallel to the axis. The status of all voxels along a ray embodies the spatial distribution of the model along this ray. Finally, theVED is computed by applying 2D Fourier transformation to the six color images. With VED, we can distinguish a hollow sphere from asolid sphere. To improve the retrieval efficiency, the database structure is optimized by an improved geometric manifold entropy(iGEOMEN) scheme. VED and iGEOMEN are integrated into a model retrieval system. Experimental results demonstrate that the VEDdescriptor outperforms the previous visual-based shape descriptors, especially for complex assembly models.

Prognostic Significance of Overexpression of EZH2 and H3k27me3 Proteins in Gastric Cancer

He, Long-Jun,Cai, Mu-Yan,Xu, Guo-Liang,Li, Jian-Jun,Weng, Zi-Jin,Xu, Da-Zhi,Luo, Guang-Yu,Zhu, Sen-Lin,Xie, Dan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

The enhancer of zeste homolog 2 (EZH2) methyl transferase and histone 3 lysine 27 (H3K27me3) protein can repress gene transcription, and their aberrant expression has been observed in various human cancers. This study determined their expression levels in gastric cancer tissues with reference to clinicopathological features and patient survival. We collected 117 gastric cancer and corresponding normal tissues for immunohistochemistry analysis. In gastric cancers, 82/117 (70.1%) were positive for EZH2 and 66/117 (56.4%) for H3K27me3 proteins in contrast to only 5.41% and 7.25% of normal gastric mucosa specimens, respectively. Kaplan-Meier survival data showed the average overall and disease-free survival of EZH2 high expression patients was 25.2 and 20.2 months, respectively, shorter than that with EZH2 low expression (40.5 and 35.9 months). The average overall survival and disease-free survival of high H3K27me3 expression patients was 23.4 and 17.4 months, shorter than without H3K27me3 expression (37.6 and 34.5 months). The average overall survival and disease-free survival of patients with both EZH2 and H3K27me3 expression was 18.8 and 12.9 months, respectively, shorter than that with either alone (34.7 and 31.2 months) or with low levels of both (43.9 and 39.9 months). Multivariate Cox regression analysis showed that H3K27me3 and EZH2 expression, tumor size differentiation and clinical stage were all independent prognostic factors for predicting patient survival. This study demonstrated that detection of both EZH2 and H3K27me3 proteins can predict poor survival of gastric cancer patients, superior to single protein detection. In addition, H3K27me3 and EZH2 protein expression could predict lymph node metastasis.