In vivo antioxidant, hypoglycemic, and anti-tumor activities of anthocyanin extracts from purple sweet potato

Jin-Ge Zhao,Qian-Qian Yan,Li-Zhen Lu,Yu-Qing Zhang 한국영양학회 2013 Nutrition Research and Practice Vol.7 No.5

Anthocyanin from purple sweet potato (PSP) extracted by microwave baking (MB) and acidified electrolyzed water (AEW) exhibited antioxidant activity. After further purification by macroporous AB-8 resin, the color value of PSP anthocyanin (PSPA) reached 30.15 with a total flavonoid concentration of 932.5 mg/g. The purified extracts had more potent antioxidant activities than the crude extracts. After continuously administering the PSP extracts to 12-mo-old mice for 1 mo, the anti-aging index of the experimental group was not significantly different from that of 5-mo-old mice. To a certain degree, PSPA was also effective for controlling plasma glucose levels in male Streptozocin (STZ)-treated diabetic mice. In addition, the extracts inhibited Sarcoma S180 cell growth in ICR mice. Mice consuming the PSP extracts formed significantly fewer and smaller sarcomas than mice consuming the control diets. The highest inhibition rate was 69.03%. These results suggest that anthocyanin extracts from PSP not only exert strong antioxidant effects in vitro, but also had anti-aging, anti-hyperglycemic, and anti-tumor activities.

CA1 Modulates the Osteogenic Differentiation of Dental Follicle Stem Cells by Activating the BMP Signaling Pathway In Vitro

Zhao Jin-ze,Ge Ying-Ying,Xue Ling-fa,Xu Yao-xiang,Yue Jin,Li Cong,Xiao Wen-lin 한국조직공학과 재생의학회 2024 조직공학과 재생의학 Vol.21 No.6

Background: Carbonic anhydrase 1 (CA1) has been found to be involved in osteogenesis and osteoclast in various human diseases, but the molecular mechanisms are not completely understood. In this study, we aim to use siRNA and lentivirus to reduce or increase the expression of CA1 in Dental follicle stem cells (DFSCs), in order to further elucidate the role and mechanism of CA1 in osteogenesis, and provide better osteogenic growth factors and stem cell selection for the application of bone tissue engineering in alveolar bone fracture transplantation. Methods: The study used RNA interference and lentiviral vectors to manipulate the expression of the CA1 gene in DFSCs during in vitro osteogenic induction. The expression of osteogenic marker genes was evaluated and changes in CA1, alkaline phosphatase (ALP), Runt-related transcription factor 2 (RUNX2), and Bone morphogenetic proteins (BMP2) were measured using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting (WB). The osteogenic effect was assessed through Alizarin Red staining. Results: The mRNA and protein expression levels of CA1, ALP, RUNX2, and BMP2 decreased distinctly in the si-CA1 group than other groups (p < 0.05). In the Lentivirus-CA1 (LV-CA1) group, the mRNA and protein expressions of CA1, ALP, RUNX2, and BMP2 were amplified to varying degrees than other groups (p < 0.05). Apart from CA1, BMP2 (43.01%) and ALP (36.69%) showed significant upregulation (p < 0.05). Alizarin red staining indicated that the LV-CA1 group produced more calcified nodules than other groups, with a higher optical density (p < 0.05), and the osteogenic effect was superior. Conclusions: CA1 can impact osteogenic differentiation via BMP related signaling pathways, positioning itself upstream in osteogenic signaling pathways, and closely linked to osteoblast calcification and ossification processes. Background: Carbonic anhydrase 1 (CA1) has been found to be involved in osteogenesis and osteoclast in various human diseases, but the molecular mechanisms are not completely understood. In this study, we aim to use siRNA and lentivirus to reduce or increase the expression of CA1 in Dental follicle stem cells (DFSCs), in order to further elucidate the role and mechanism of CA1 in osteogenesis, and provide better osteogenic growth factors and stem cell selection for the application of bone tissue engineering in alveolar bone fracture transplantation. Methods: The study used RNA interference and lentiviral vectors to manipulate the expression of the CA1 gene in DFSCs during in vitro osteogenic induction. The expression of osteogenic marker genes was evaluated and changes in CA1, alkaline phosphatase (ALP), Runt-related transcription factor 2 (RUNX2), and Bone morphogenetic proteins (BMP2) were measured using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting (WB). The osteogenic effect was assessed through Alizarin Red staining. Results: The mRNA and protein expression levels of CA1, ALP, RUNX2, and BMP2 decreased distinctly in the si-CA1 group than other groups (p < 0.05). In the Lentivirus-CA1 (LV-CA1) group, the mRNA and protein expressions of CA1, ALP, RUNX2, and BMP2 were amplified to varying degrees than other groups (p < 0.05). Apart from CA1, BMP2 (43.01%) and ALP (36.69%) showed significant upregulation (p < 0.05). Alizarin red staining indicated that the LV-CA1 group produced more calcified nodules than other groups, with a higher optical density (p < 0.05), and the osteogenic effect was superior. Conclusions: CA1 can impact osteogenic differentiation via BMP related signaling pathways, positioning itself upstream in osteogenic signaling pathways, and closely linked to osteoblast calcification and ossification processes.

Influence of temperature on the development, reproduction, and life table of Calliptamus italicus (L.) (Orthoptera: Acridoidea)

Jin-Long Ren,Xiongbing Tu,Jing Ge,Li Zhao,Zehua Zhang 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.1

Calliptamus italicus is a migratory species of grasshopper that can cause serious damage to grasslands in Xinjiang, China. In this study,we examined the effects of different temperatures (23, 26, 29, 32, and 35 °C) on the lifecycle and life table parameters. The results showed that temperature strongly influences the growth and development of C. italicus. At temperatures ranging from 23 to 35 °C, the development time was negatively linearly related to temperature (P b 0.01). The time of development from an egg to an adult (pre-oviposition) ranged from86.8 d at 26 °C to 40.6 d at 35 °C, and no oviposition occurred at 23 °C. An average of 687.1 degree-days was required for complete development to occur above the lower threshold temperature (18.3 °C). Egg hatching rates and nymph and pre-adult survival rates were lowest at 23 °C (95.6%, 72.6%, and 0%, respectively) and highest at 32 °C (97.8%, 86.2%, and 88.7%, respectively). The mean total fecundity ranged from 41 eggs/female at 26 °C to 55 eggs/female at 32 °C. The sex ratio (% females) ranged from30 to 52%. The intrinsic rate of population increase (rm) at the different temperatures ranged from 0.0020 to 0.0447 d−1, with the highest value recorded at 32 °C. These results indicate that temperature significantly affects the biology of C. italicus and that the optimal temperature range for its development is 26–32 °C.

Structural and Ferromagnetic Properties SnO2/TiO2 Nanotubes Obtained by Electrospinning

JIAN-GUO ZHAO,Yan-Hong Gu,Hong Jia,Zhuan Hu,Shuqian Qiao,Mengjing Jin,Panting Ge,WEI-YING ZHANG,Shijiang Liu,ZHAO-JUN LIU 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.10

SnO2/TiO2 (Sn/Ti = 5/1) nanotubes were prepared by the electrospinning method. The morphology was characterized by scanning electron microscope (SEM) and transmission electron microscope (TEM). The results showed that the inner diameter of prepared materials was about 100 nm and the wall thickness was about 10 nm. The results of X-ray diffraction (XRD) and high resolution transmission electron microscope (HRTEM) showed that SnO2/TiO2 nanotubes had a mixed phase of SnO2 rutile and TiO2 anatase structures and no impurity phases. The magnetic properties of the SnO2/TiO2 nanotubes were characterized by a superconducting quantum interferometer (SQUID). The results indicated that the samples exhibited room temperature ferromagnetism which may be attributed to the interface between TiO2 and SnO2 phases.

MICROCOMPUTER AIDED TXPERIMENT (MCAE) SYSTEM OF DISPATCHED WINCH

Huang Tai Shan,Ge Yan Jin,Pan Hong Zhao 대한전자공학회 1991 ICEIC:International Conference on Electronics, Inf Vol.1991 No.8

Microcomputer Aided Txperiment ( MCAE ) System of Dispatched Winch

Huang Tai Shan,Ge Yan Jin,Pan Hong Zhao 대한전자공학회 1991 International Conference on Electronics, Informati Vol.1 No.1

The Beneficial Effects of Electroacupuncture at PC6 Acupoints (Neiguan) on Myocardial Ischemia in ASIC3 / mice

Ying-Wang,Yi-guo Chen,Wan-shuang Zhao,Di Li,Ya-han Xu,Meng-di Li,Jin Chen,Zhi-jun Kou,Qi-ge Wang,Nsoa dimitri Joseph 사단법인약침학회 2018 Journal of Acupuncture & Meridian Studies Vol.11 No.3

This study aims to investigate the possible mechanisms of electroacupuncture (EA) at PC6to improve myocardial ischemia (MI) by regulating the cardiac transient outward potassiumcurrent channel (Ito). According to the random number table, the mice were dividedinto six groups of six mice each: control group, MI group, PC6, LU7 (Lieque-point), ST36(Zusanli-point), and nonacupoint group. Mice in the control group were injected with saline(20 mg/kg, 24 hours interval), and the other ASIC3 / mice were injected subcutaneouslytwice with isoproterenol (ISO) (20 mg/kg, 24 hours interval). In thepreexperiment, 5 mg/kg, 10 mg/kg, 20 mg/kg, and 30 mg/kg of ISO were used, andthe results showed that 5 mg/kg and 10 mg/kg of ISO both could induce acute MI, butshorter duration of sustained MI. On the other hand, an injection of 30 mg/kg can makethe mice experience arrhythmia or die immediately, and EA was operated at PC6, LU7,ST36 acupoints, and nonacupoint in the mice of PC6, LU7, ST36, and nonacupoint groups,respectively, after injecting twice. Then Western blotting techniques (Western Blot) wereused to analyze the protein expressions of Kv1.4, Kv4.2, Kv4.3, and KchIP2. The results ofthis experiment showed that the protein expressions of Kv1.4, Kv4.2, Kv4.3, and KChIP2 inMI group were significantly lower than those in the control group (p < 0.01). Comparedwith MI group, the results of PC6, LU7, and ST36 groups obviously increased (p < 0.05). Furthermore, the expressions of PC6 group were higher than LU7 group and ST36 group(p < 0.05). And electrocardiogram’s T-waves showed obvious pathological changes inthe MI group compared to the control group (p < 0.01). After EA, the abnormal T-waves voltage of ECG in PC6, LU7, and ST36 groups was improved (p < 0.05). In addition, therate change of PC6 group was larger than that of both LU7 and ST36 groups (p < 0.05). But the T-waves voltage of the nonacupoint group was not significantly different than thatof the MI group (p > 0.05).

A detection method of Escherichia coli O157:H7 based on immunomagnetic separation and aptamers-gold nanoparticle probe quenching Rhodamine B’s fluorescence

Fengnan Lian,Dan Wang,Shuo Yao,Lirui Ge,Yue Wang,Yuyi Zhao,Jinbin Zhao,Xiuling Song,Chao Zhao,Jinhua Li,Yajuan Liu,Minghua Jin,Kun Xu 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.8

This research aimed to detect Escherichia coli O157:H7 in milk based on immunomagnetic probe separation technology and quenching effect of gold nanoparticles to Rhodamine B. Streptavidin-modified magnetic beads (MBs) were combined with biotin-modified antibodies to capture E. coli O157:H7 specifically. Gold nanoparticle (AuNPs) was incubated with sulfhydryl-modified aptamers (SH-Aptamers) to obtain the Aptamers-AuNPs probe. After magnetic beads captured target bacteria and formed a sandwich structure with the gold nanoprobe, Rhodamine B was added into complex to obtain fluorescent signal changes. Our results demonstrated that the established method could detect E. coli O157:H7 in the range of 101–107 CFU/mL, and the limit of detection (LOD) was 0.35 CFU/mL in TBST buffer (pH = 7.4). In milk simulation samples, the LOD of this method was 1.03 CFU/mL. Our research provides a promising approach on the detection of E. coli O157:H7.

Control effect and mechanism investigation on the horizontal flow-isolating plate for PI shaped bridge decks’ VIV stability

Ke Li,Guowei Qian,Yaojun Ge,Lin Zhao,Jin Di 한국풍공학회 2019 Wind and Structures, An International Journal (WAS Vol.28 No.2

Vortex-Induced-Vibration (VIV) is one kind of the wind-induced vibrations, which may occur in the construction and operation period of bridges. This phenomenon can bring negative effects to the traffic safety or can cause bridge fatigue damage and should be eliminated or controlled within safe amplitudes. In the current VIV studies, one available mitigation countermeasure, the horizontal flow-isolating plate, shows satisfactory performance particularly in PI shaped bridge deck type. Details of the wind tunnel test are firstly presented to give an overall description of this appendage and its control effect. Then, the computational-fluid-dynamics (CFD) method is introduced to investigate the control mechanism, using two-dimensional Large-Eddy-Simulation to reproduce the VIV process. The Reynolds number of the cases involved in this paper ranges from 1×〖10〗^5 to 3×〖10〗^5, using the width of bridge deck as reference length. A field-filter technique and detailed analysis on wall pressure are used to give an intuitive demonstration of the changes brought by the horizontal flow-isolating plate. Results show that this aerodynamic appendage is equally effective in suppressing vertical and torsional VIV, indicating inspiring application prospect in similar PI shaped bridge decks.

Clinical Outcomes of Downregulation of E-cadherin Gene Expression in Non-small Cell Lung Cancer

Zheng, Shi-Ying,Hou, Jing-Yu,Zhao, Jun,Jiang, Dong,Ge, Jin-Feng,Chen, Sheng Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Objective: To investigate the promoter methylation status of the E-cadherin gene in non-small cell lung cancer (NSCLC) and its association with clinical pathological parameters, and to explore the relationship between downregulation of E-cadherin gene expression and the methylation status of its promoter region. Methods: Nested methylation-specific PCR was performed to examine CpG methylation within the 5' CpG island of the E-cadherin gene in lung cancer and para-cancerous tissue from 37 patients with primary non-small cell lung cancer. Quantitative real-time PCR was performed to measure the level of E-cadherin mRNA. Results: Of thirty-seven cases, 12 (32.4%) samples showed aberrant CpG methylation in tumor tissues compared with the corresponding normal tissues. In addition, a reduction in E-cadherin mRNA levels was observed in 11 of the 12 (91.7%) tumor tissues carrying a methylated E-cadherin gene. However, only 10 (43.5%) cases displayed reduced mRNA levels in tumor tissues from the remaining 23 cases (excluding 2 samples from which mRNA was unavailable) without methylation events. Downregulation of E-cadherin gene expression significantly correlated with the promoter methylation status of this gene. Conclusion: These results provide strong evidence that the methylation status of E-cadherin gene contributes to a reduction in the expression of E-cadherin mRNA, and may play a role in the development and progression of NSCLC.