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      • Fractional Differentiation-based Image Feature Extraction

        Xiangwei Xu,Fang Dai,Wenyan Guo,Jianmin Long 보안공학연구지원센터(IJSIP) 2014 International Journal of Signal Processing, Image Vol.7 No.6

        Two novel methods for image feature extraction based on fractional differentiation are presented in this paper. The first method is the feature extraction of fusing multi-direction CRONE operators. In this method, the fractional differential CRONE mask is generalized to eight directions at first for extracting image features; then the extracted features are tested by the statistic method and fused by the gradient ratio, so that the outlines of the objects in the image are obtained. In order to extract the detail feature information in the image effectively, the second method, the ‘S+Z’ extraction combined with the space-filling curves, is presented. By introducing the space-filling curves, the ‘S’ curve and the ‘Z’ curve, and making full use of the neighborhood information of image pixels, the detailed features of the objects in the image are obtained. The experiment results show that our methods can obtain satisfactory image features.

      • KCI등재
      • An Improved DV-Hop Localization Algorithm Based on the Node Deployment in Wireless Sensor Networks

        Jianmin Zhang,Ning Guo,Jian Li 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.10

        Localization algorithm is an important and challenging topic in Wireless Sensor Networks (WSNs), especially for the applications requiring the accurate position of the sensed information. In this paper, an improved DV-Hop algorithm based on the node deployment was proposed. The proposed algorithm improves the localization accuracy by taking the advantage of the node deployment when computing the distance between the unknown node and the anchor. Simulation results prove that the improved DV-Hop algorithm offers the better performance compared to the original DV-Hop algorithm in localization accuracy without requiring additional hardware.

      • KCI등재

        Electroacupuncture-Modulated MiR106b-5p Expression Enhances Autophagy by Targeting Beclin-1 to Promote Motor Function Recovery After Spinal Cord Injury in Rats

        Shuhui Guo,Jianmin Chen,Ye Yang,Xiaolu Li,Yun Tang,Yuchang Gui,Jianquan Chen,Jianwen Xu 대한척추신경외과학회 2023 Neurospine Vol.20 No.3

        Objective: Electroacupuncture (EA) has a definite effect on the treatment of spinal cord injuries (SCIs), but its underlying molecular mechanism remains unclear. Meanwhile, MiR-106b-5p is an autophagy- and apoptosis-related microribonucleic acid, but whether it regulates the progression of autophagy and apoptosis in SCIs is yet undetermined. As such, this study aimed to elucidate the involvement of miR-106b-5p in the EA treatment of an SCI. Methods: The miR-106b-5p level was detected by quantitative real-time polymerase chain reaction. In vitro, SH-SY5Y cells were transfected with miR-106b-5p mimics or inhibitors to regulate the miR-106b-5p expression, while in vivo, SCI rats were treated with EA for 7 days at the bilateral Zusanli (ST36) and Jiaji (EX-B2) acupoints. The motor function was evaluated using the Basso-Beattie-Bresnahan (BBB) criteria. Further, autophagic vacuoles, pathological damage, and neuronal cell morphology were observed by transmission electron microscopy, as well as by hematoxylin and eosin and Nissl staining, respectively. Results: The miR-106b-5p level, which can interact directly with Beclin-1 by influencing its expression, as well as the expressions of P62, Caspase-3, and Bax, was upregulated after an SCI, but it decreased after EA. Moreover, the ratio of LC3-II to LC3-I was upregulated after EA. EA can enhance autophagy, reduce neuronal apoptosis, and minimize motor dysfunction and histopathological deficits after an SCI. More importantly, however, all the above effects induced by EA can be reversed after an injection of miR-106-5p agomir to produce an overexpression of miR-106b-5p. Conclusion: EA treatment could downregulate miR-106b-5p to alleviate SCI-mediated injuries by promoting autophagy and inhibiting apoptosis.

      • SCIESCOPUSKCI등재

        Effects of Dietary Iodine and Selenium on the Activities of Blood Lymphocytes in Laying Hens

        Song, Zhigang,Guo, Yuming,Yuan, Jianmin Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.5

        The effect of dietary iodine and selenium supplementation, alone or in combination, on peripheral blood lymphocyte function was determined in laying hens. Eight-hundred-and-sixty-four New-Loman laying hens were randomly allotted into 12 dietary treatments with different inclusion levels of iodine (0, 0.1 and 0.2 mg/kg), selenium (0, 0.05, 0.1 and 0.2 mg/kg) or their combinations for 24 weeks. The lipopolysaccharide (LPS) stimulation index, concanavalin A (ConA) stimulation index, peroxide enzyme activity and phagocytosis to neutral red particles were tested. There were significant differences in LPS stimulation index, ConA stimulation index, peroxide enzyme activity and phagocytosis to neutral red particles in different iodine or selenium supplementation levels (p<0.05). The highest iodine and selenium supplementation both resulted in highest LPS-/ConA-stimulation indices (p<0.05). However, when iodine was lower than 0.2 mg/kg, the additional effect of different levels of selenium did not always result in significant differences in these indices. The results indicated that iodine and selenium may affect immunity in laying hens and, when the iodine level in the laying hen is lower than 0.2 mg/kg, a selenium allowance higher than 0.1 mg/kg may be necessary to improve immunity.

      • SCIESCOPUSKCI등재

        Effect of Dietary β-1,3/1,6-glucan Supplementation on Growth Performance, Immune Response and Plasma Prostaglandin E<sub>2</sub>, Growth Hormone and Ghrelin in Weanling Piglets

        Wang, Zhong,Guo, Yuming,Yuan, Jianmin,Zhang, Bingkun Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.5

        The experiment was conducted to evaluate the effect of ${\beta}$-1,3/1,6-glucan on growth performance, immunity and endocrine responses of weanling piglets. One hundred and eighty weanling piglets (Landrace$\times$Large White, $7.20{\pm}0.25kg$ BW and $28{\pm}2$ d of age) were randomly fed 1 of 5 treatment diets containing dietary ${\beta}$-1,3/1,6-glucan supplemented at 0, 25, 50, 100 and 200 mg/kg for 4 wks. Each treatment was replicated in 6 pens containing 6 pigs per pen. On d 14 and 28, body weight gain, feed consumption and feed efficiency were recorded as measures of growth performance. Peripheral blood lymphocyte proliferation and serum immunoglobulin G (IgG) were measured to study the effect of dietary ${\beta}$-1,3/1,6-glucan supplementation on immune function. Plasma prostaglandin E2 (PGE2), growth hormone (GH) and ghrelin were measured to investigate endocrine response to ${\beta}$-1,3/1,6-glucan supplementation. Our results suggest that average daily gain (ADG) and feed efficiency had a quadratic increase trend with dietary ${\beta}$-1,3/1,6-glucan supplementation from d 14 to 28, whereas it had no significant effect on average daily feed intake (ADFI). The treatment group fed with 50 mg/kg dietary ${\beta}$-1,3/1,6-glucan supplementation showed a numerical increase in ghrelin, a similar change trend with ADG and no significant effect on GH. Lymphocyte proliferation indices, serum IgG and plasma PGE2 concentrations varied linearly with dietary supplementation levels of ${\beta}$-1,3/1,6-glucan on d 14. Higher levels of ${\beta}$-1,3/1,6-glucan may have a transient immuno-enhancing effect on the cellular and humoral immune function of weanling piglets via decreased PGE2. Taking into account both immune response and growth performance, the most suitable dietary supplementation level of ${\beta}$-1,3/1,6-glucan is 50 mg/kg for weanling piglets.

      • SCIESCOPUSKCI등재

        Effects of age on intestinal phosphate transport and biochemical values of broiler chickens

        Li, Jianhui,Yuan, Jianmin,Miao, Zhiqiang,Guo, Yuming Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.2

        Objective: The objective of this experiment was to characterize the mRNA expression profile of type IIb sodium-inorganic phosphate cotransporter (NaPi-IIb) and the biochemical values of serum alkaline phosphatase (AKP), calcium, inorganic phosphorus, tibial ash and minerals of broiler chickens with aging. Methods: A total of 56 one-day-old Arbor Acres male broiler chickens were used. Broiler chickens were weighed and samples were collected weekly from day 1. Results: The result showed that before the growth inflection point, ash, calcium, and phosphorus content in the tibia of broiler chickens increased with growth (before 3 weeks of age), although there were no significant differences in chicks at different ages in the later period of the experiment and weight gain rate was relatively slow at this stage (4 to 6 weeks). NaPi-IIb gene expression in the small intestine in the early growth stage was higher than that in the later growth stage. Expression of calbindin and the vitamin D receptor protein in the intestinal mucosa increased with age in the duodenum and jejunum. Serum AKP activity first increased and subsequently decreased after peaking at 1 week of age, but there was no significant difference after 3 weeks of age. Conclusion: These results show that compared with the early growth stage, the weight-gain rate of broiler chickens in the late growth stage gradually decreased with gradual tibia maturation, along with weaker positive transport of phosphorus in the intestine and reinforced re-absorption of phosphorus in the kidney, which might be the reason that phosphorus requirement in the late growth stage was decreased.

      • The Relationship between Watching Motivation, Perceived Value, Place Attachment and Behavioral Loyalty: A Case of China Tennis Open

        ( Qiang Zhang ),( Zhibo Tian ),( Jianmin Shi ),( Wangda Guo ) 아시아스포츠융합과학회 2021 Asia Pacific Journal of Applied Sport Sciences Vol.2 No.2

        PURPOSE The purpose of this study is to investigate a behavioral loyalty model of China Tennis Open spectators and validate the relationships among spectators’’ watching motivation, perceived value, place attachment and behavioral loyalty. METHOD The spectators at the China Tennis Open were targeted as the main research sample. A total of 400 questionnaires were collected. The collected data was analyzed by means of descriptive statistics, confirmatory factor analysis, structural equation modeling, and significance test of difference. RESULT (1) Motivation has a positive effect on perceived value and place attachment, but the behavioral loyalty is not affected. (2) Perceived value directly influence on behavioral loyalty. (3) Place attachment directly influence on behavioral loyalty. CONCLUSION Motivation has a limited influence on the behavioral loyalty. Place attachment and perceived value are important factors in behavioral loyalty of China Tennis Open spectators. The results of this study provides some practical suggestions for the organization, management of tennis events and a direction for future research.

      • KCI등재

        Genistein activates endothelial nitric oxide synthase in broiler pulmonary arterial endothelial cells by an Akt-dependent mechanism

        Ying Yang,Wei Nie,Jianmin Yuan,Bingkun Zhang,Zhong Wang,Zhenlong Wu,Yuming Guo 생화학분자생물학회 2010 Experimental and molecular medicine Vol.42 No.11

        Deregulation of endothelial nitric oxide synthase (eNOS)plays an important role in the development of multiple cardiovascular diseases. Our recent study demonstrated that genistein supplementation attenuates pulmonary arterial hypertension in broilers by restoration of endothelial function. In this study, we investigated the molecular mechanism by using broiler pulmonary arterial endothelial cells (PAECs). Our results showed that genistein stimulated a rapid phosphorylation of eNOS at Ser1179 which was associated with activation of eNOS/NO axis. Further study indicated that the activation of eNOS was not mediated through estrogen receptors or tyrosine kinase inhibition, but via a phosphatidylinositol 3-kinase (PI3K)/Akt-dependent signaling pathway, as the eNOS activity and related NO release were largely abolished by pharmacological inhibitors of PI3K or Akt. Thus, our findings revealed a critical function of Akt in mediating genistein-stimulated eNOS activity in PAECs, partially accounting for the beneficial effects of genistein on the development of cardiovascular diseases observed in animal models.

      • KCI등재

        Effect of Zinc on Growth Performance, Gut Morphometry, and Cecal Microbial Community in Broilers Challenged with Salmonella enterica serovar Typhimurium

        Yuxin Shao,Zhao Lei,Jianmin Yuan,Ying Yang,Yuming Guo,Bingkun Zhang 한국미생물학회 2014 The journal of microbiology Vol.52 No.12

        To evaluate the effects of supplemental zinc on growth performance,gut morphometry, and the cecal microbial communityin broilers challenged with Salmonella typhimurium,180, 1-day-old male Cobb 500 broiler chicks were randomlyassigned to 3 treatments with ten replicates for a 42 day experiment. The 3 treatments were: unchallenged, S. typhimurium-challenged, and S. typhimurium-challenged with 120mg/kg of zinc supplementation in the diet. Salmonella infectioncaused a reduction in body-weight gain and feed intake,disrupted the intestinal structure by decreasing thevillus-height/crypt-depth ratio of the ileum and increasingthe apoptotic index of ileal epithelial cells. Moreover, thececal microbial community was altered by Salmonella infection,as demonstrated by a reduced number of Lactobacillusand total bacteria. Dietary zinc supplementation improvedgrowth performance by increasing the body-weight gainand feed intake in the challenged broilers. In addition, zincrepaired intestinal injury by reducing the apoptotic index ofileal epithelial cells, enhancing villus height and the villusheight/crypt-depth ratio of the ileum, and the proliferationindex of ileal epithelial cells. Finally, zinc regulated the cecalmicrobial community by increasing the number of total bacteriaand beneficial Lactobacillus bacteria, and reducing thenumber of Salmonella. The results indicated that dietaryzinc supplementation improved growth performance, intestinalmorphology, and intestinal microbiota in S. typhimurium-challenged broilers.

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