The Growth-promoting Effect of Tetrabasic Zinc Chloride is Associated with Elevated Concentration of Growth Hormone and Ghrelin

Zhang, Bingkun,Guo, Yuming Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.10

An experiment was conducted to investigate the mechanism for the effect of tetrabasic zinc chloride (TBZC) in enhancing growth performance of weanling piglets. Gut-brain peptides play an important role in the regulation of growth and appetite in animals. This study evaluated the effects of TBZC on blood concentrations of growth hormone (GH), ghrelin, insulin-like growth factor-I (IGF-I), cholecystokinin (CCK) and neuropeptide Y (NPY). Seventy-two weanling piglets (Landrace$\times$Large White) with an initial body weight (BW) of $6.7{\pm}0.16kg$ and aged $24{\pm}1days$ were assigned to three dietary treatments: i) control diet without TBZC supplement, ii) the control diet supplemented with 2,000 mg Zn from TBZC/kg and iii) TBZC-supplemented diet pair-fed with respect to the control diet. Each treatment had six replications (pens) of four piglets. At the end of a 14-d experimental period, piglets were weighed and feed consumption was measured, and blood samples were collected for assays of GH, ghrelin, IGF-I, CCK and NPY concentrations. The inclusion of TBZC in the diet increased average daily gain (p<0.01), average daily feed intake (p<0.05), and feed conversion ratio (p<0.05). Pair-fed piglets had higher ADG, and lower FCR than (p<0.05) Control piglets. Supplementation of the diet with TBZC increased (p<0.05) serum GH and plasma ghrelin levels in weanling piglets, but did not affect (p>0.05) serum IGF-I and plasma NPY and CCK concentrations. Pair-fed piglets had lower (p<0.05) serum GH levels than TBZC-supplemented piglets, but did not (p>0.05) differ from Control piglets. These data indicated that TBZC elevated the concentration of ghrelin and GH. This observation may partly explain the beneficial effects of TBZC on growth performance of weanling piglets.

Enhanced Li storage performance of ordered mesoporous MoO2 via tungsten doping.

Fang, Xiangpeng,Guo, Bingkun,Shi, Yifeng,Li, Bin,Hua, Chunxiu,Yao, Chaohua,Zhang, Yichi,Hu, Yong-Sheng,Wang, Zhaoxiang,Stucky, Galen D,Chen, Liquan RSC Pub 2012 Nanoscale Vol.4 No.5

<P>Ordered mesoporous tungsten-doped MoO(2) was synthesized by a nanocasting method. The Li storage performance of mesoporous MoO(2) is significantly improved by tungsten doping, which exhibits a reversible capacity of 700 mA h g(-1), better cycling and rate performance. This material combines the advantages of the high theoretical capacity of MoO(2) and the better electroactivity of WO(2).</P>

Genistein activates endothelial nitric oxide synthase in broiler pulmonary arterial endothelial cells by an Akt-dependent mechanism

Yang, Ying,Nie, Wei,Yuan, Jianmin,Zhang, Bingkun,Wang, Zhong,Wu, Zhenlong,Guo, Yuming Korean Society for Biochemistry and Molecular Bion 2010 Experimental and molecular medicine Vol.42 No.11

Deregulation of endothelial nitric oxide synthase (eNOS) plays an important role in the development of multiple cardiovascular diseases. Our recent study demonstrated that genistein supplementation attenuates pulmonary arterial hypertension in broilers by restoration of endothelial function. In this study, we investigated the molecular mechanism by using broiler pulmonary arterial endothelial cells (PAECs). Our results showed that genistein stimulated a rapid phosphorylation of eNOS at $Ser^{1179}$ which was associated with activation of eNOS/NO axis. Further study indicated that the activation of eNOS was not mediated through estrogen receptors or tyrosine kinase inhibition, but via a phosphatidylinositol 3-kinase (PI3K)/Akt-dependent signaling pathway, as the eNOS activity and related NO release were largely abolished by pharmacological inhibitors of PI3K or Akt. Thus, our findings revealed a critical function of Akt in mediating genistein-stimulated eNOS activity in PAECs, partially accounting for the beneficial effects of genistein on the development of cardiovascular diseases observed in animal models.

Preparation of superparamagnetic ZnFe₂O₄ submicrospheres via a solvothermal method

Ma, Jie,Chen, Bingjie,Chen, Bingkun,Zhang, Shuping Techno-Press 2017 Advances in nano research Vol.5 No.2

Superparamagnetic Zinc ferrite submicropheres are firstly synthesized via a one-pot solvothermal approach at $200-215^{\circ}C$ for 4-8 hours. $ZnCl_2$, $FeCl_3$ and NaAc are used as precursors with ethylene glycol solvent. The X-ray diffraction (XRD) data indicate that $ZnFe_2O_4$ nanoparticles with the grain size around $15{\pm}3nm$ can be successfully synthesized via the one-pot method. The scanning/transmission electronic microscope (SEM/TEM) images further show the samples are submicrospheres self-assembled by nanoparticles with size about 375-500 nm changed with reaction conditions. Room-temperature vibration magnetic strength measurements (VMS) demonstrates the as-obtained $ZnFe_2O_4$ submicrospheres show prefect superparamagnetism, whose coercivity force and remanence are practically nil. The reaction temperature and time influence on the crystallinity, diameter, saturated magnetic intensity and morphology of the particles.

Genistein activates endothelial nitric oxide synthase in broiler pulmonary arterial endothelial cells by an Akt-dependent mechanism

Ying Yang,Wei Nie,Jianmin Yuan,Bingkun Zhang,Zhong Wang,Zhenlong Wu,Yuming Guo 생화학분자생물학회 2010 Experimental and molecular medicine Vol.42 No.11

Deregulation of endothelial nitric oxide synthase (eNOS)plays an important role in the development of multiple cardiovascular diseases. Our recent study demonstrated that genistein supplementation attenuates pulmonary arterial hypertension in broilers by restoration of endothelial function. In this study, we investigated the molecular mechanism by using broiler pulmonary arterial endothelial cells (PAECs). Our results showed that genistein stimulated a rapid phosphorylation of eNOS at Ser1179 which was associated with activation of eNOS/NO axis. Further study indicated that the activation of eNOS was not mediated through estrogen receptors or tyrosine kinase inhibition, but via a phosphatidylinositol 3-kinase (PI3K)/Akt-dependent signaling pathway, as the eNOS activity and related NO release were largely abolished by pharmacological inhibitors of PI3K or Akt. Thus, our findings revealed a critical function of Akt in mediating genistein-stimulated eNOS activity in PAECs, partially accounting for the beneficial effects of genistein on the development of cardiovascular diseases observed in animal models.

Effect of Dietary β-1,3/1,6-glucan Supplementation on Growth Performance, Immune Response and Plasma Prostaglandin E₂, Growth Hormone and Ghrelin in Weanling Piglets

Wang, Zhong,Guo, Yuming,Yuan, Jianmin,Zhang, Bingkun Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.5

The experiment was conducted to evaluate the effect of ${\beta}$-1,3/1,6-glucan on growth performance, immunity and endocrine responses of weanling piglets. One hundred and eighty weanling piglets (Landrace$\times$Large White, $7.20{\pm}0.25kg$ BW and $28{\pm}2$ d of age) were randomly fed 1 of 5 treatment diets containing dietary ${\beta}$-1,3/1,6-glucan supplemented at 0, 25, 50, 100 and 200 mg/kg for 4 wks. Each treatment was replicated in 6 pens containing 6 pigs per pen. On d 14 and 28, body weight gain, feed consumption and feed efficiency were recorded as measures of growth performance. Peripheral blood lymphocyte proliferation and serum immunoglobulin G (IgG) were measured to study the effect of dietary ${\beta}$-1,3/1,6-glucan supplementation on immune function. Plasma prostaglandin E2 (PGE2), growth hormone (GH) and ghrelin were measured to investigate endocrine response to ${\beta}$-1,3/1,6-glucan supplementation. Our results suggest that average daily gain (ADG) and feed efficiency had a quadratic increase trend with dietary ${\beta}$-1,3/1,6-glucan supplementation from d 14 to 28, whereas it had no significant effect on average daily feed intake (ADFI). The treatment group fed with 50 mg/kg dietary ${\beta}$-1,3/1,6-glucan supplementation showed a numerical increase in ghrelin, a similar change trend with ADG and no significant effect on GH. Lymphocyte proliferation indices, serum IgG and plasma PGE2 concentrations varied linearly with dietary supplementation levels of ${\beta}$-1,3/1,6-glucan on d 14. Higher levels of ${\beta}$-1,3/1,6-glucan may have a transient immuno-enhancing effect on the cellular and humoral immune function of weanling piglets via decreased PGE2. Taking into account both immune response and growth performance, the most suitable dietary supplementation level of ${\beta}$-1,3/1,6-glucan is 50 mg/kg for weanling piglets.

Effect of Zinc on Growth Performance, Gut Morphometry, and Cecal Microbial Community in Broilers Challenged with Salmonella enterica serovar Typhimurium

Yuxin Shao,Zhao Lei,Jianmin Yuan,Ying Yang,Yuming Guo,Bingkun Zhang 한국미생물학회 2014 The journal of microbiology Vol.52 No.12

To evaluate the effects of supplemental zinc on growth performance,gut morphometry, and the cecal microbial communityin broilers challenged with Salmonella typhimurium,180, 1-day-old male Cobb 500 broiler chicks were randomlyassigned to 3 treatments with ten replicates for a 42 day experiment. The 3 treatments were: unchallenged, S. typhimurium-challenged, and S. typhimurium-challenged with 120mg/kg of zinc supplementation in the diet. Salmonella infectioncaused a reduction in body-weight gain and feed intake,disrupted the intestinal structure by decreasing thevillus-height/crypt-depth ratio of the ileum and increasingthe apoptotic index of ileal epithelial cells. Moreover, thececal microbial community was altered by Salmonella infection,as demonstrated by a reduced number of Lactobacillusand total bacteria. Dietary zinc supplementation improvedgrowth performance by increasing the body-weight gainand feed intake in the challenged broilers. In addition, zincrepaired intestinal injury by reducing the apoptotic index ofileal epithelial cells, enhancing villus height and the villusheight/crypt-depth ratio of the ileum, and the proliferationindex of ileal epithelial cells. Finally, zinc regulated the cecalmicrobial community by increasing the number of total bacteriaand beneficial Lactobacillus bacteria, and reducing thenumber of Salmonella. The results indicated that dietaryzinc supplementation improved growth performance, intestinalmorphology, and intestinal microbiota in S. typhimurium-challenged broilers.