The effects of methionine on TCE-induced DNA methylation and mRNA expression changes in mouse liver

Jiahong Chen,Hang Zhang,Stanley Aniagu,Yan Jiang,Tao Chen,Y. Jiang,T. Chen 대한독성 유전단백체 학회 2017 Molecular & cellular toxicology Vol.13 No.1

Trichloroethylene (TCE) had been shown to induce aberrant DNA methylation changes in mouse liver, which could be critical in the development of hepatocellular carcinoma. To understand the effect of methionine, a methyl donor, on TCE-induced mouse liver cancer, we exposed B6C3F1 mice to TCE in the presence or absence of methionine. We found that supplementation with methionine attenuated the TCE-induced hypo- and hyper-methylation status of Cdkn1a and Ihh in mouse liver, respectively. The TCE-induced mRNA expression changes of Cdkn1a, Ihh and Jun were also alleviated by methionine. However, methionine supplementation accelerated the TCE-induced mRNA over-expression of Mki67, and enhanced the TCE-induced mRNA down-regulation of Dnmt3a and Tet2. In summary, our results showed that methionine could attenuate at least part of the TCE-induced DNA methylation changes, but the additional gene expression changes induced by supplementary of methionine does not support the assumption that methionine could prevent TCE-induced liver carcinogenesis.

Estimation of Energy and Time Savings in Optical Glass Manufacturing When Using Ultrasonic Vibration‑Assisted Grinding

Chen Jiang,Tao Wu,Hui Ye,Jinyi Cheng,Yu Hao 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.6 No.1

Energy and time savings are highly important aspects of green manufacturing. Ultrasonic vibration-assisted grinding (UVAG) is a high-efficiency, low-energy-consumption processing method for optical components made from hard and brittle materials. This work presents an experimental investigation of the specific grinding energy and the subsurface damage depth in UVAG of optical glasses to estimate the increased energy and time savings produced when using UVAG in optical glass manufacturing. The normal and tangential grinding forces of traditional grinding (TG) and axial UVAG processes on optical glasses were investigated for various machining parameters. The specific grinding energies during the TG and UVAG of the optical glasses were calculated and analyzed from the perspective of the energy consumption of the grinding process. The subsurface damage depths in optical glass during TG and UVAG were measured as an estimate of the machining quality, and the magnetorheological polishing spot method was used to analyze the time saved in subsequent polishing processes. The results show that UVAG can reduce energy consumption during the grinding of glass and produce significant time savings in subsequent polishing processes. The UVAG process therefore shows good potential for use in green manufacturing of optical components.

Psychosocial Factors Associated With Irritable Bowel Syndrome Development in Chinese College Freshmen

Chen Jiang,Yan Xu,Stuti Sharma,Lei Zhang,Jun Song,Wei Qian,Tao Bai,Xiaohua Hou 대한소화기 기능성질환∙운동학회 2019 Journal of Neurogastroenterology and Motility (JNM Vol.25 No.2

Background/Aims The role of psychosocial factors on irritable bowel syndrome (IBS) in adolescents is incompletely understood. The aim of this study is to investigate the association between the psychosocial factors and the risk of developing IBS in college freshmen. Methods A cross-sectional survey was conducted in a randomly selected freshmen population in Wuhan China (n = 2449). Questionnaire evaluated demographics and psychosocial risks. The population was divided into 3 groups: non-discomfort, chronic abdominal discomfort and IBS. The association between the development of IBS and psychosocial factors was analyzed by ordinal and multiple logistic regression analysis. Results A total of 2053 (83.8%) completed this survey (mean age, 18.2 ± 0.9 years; female, 35.6%). Among them, 82 (4.0%) fulfilled the Rome III criteria for IBS. Female (odds ratio [OR], 3.31; 95% confidence interval [CI], 2.47–4.45), experience of abuse (OR, 2.44; 95% CI, 1.10–5.56), and suicidal intention (OR, 2.17; 95% CI, 1.15–4.17) were more likely to have IBS. Compared with chronic abdominal discomfort, however, depression (OR, 5.55; 95% CI, 1.36–22.71) was the only dependent risk factor for IBS. Conclusion The prevalence of IBS in college freshmen is 4.0%, and to the freshmen, psychosocial factors such as experience of abuse, depression, and suicidal intention were associated with high risk of developing IBS.

Physics-informed Gaussian process probabilistic modeling for prognostics of degradation processes

Chen Jiang,Hyunhee Choi,Byeng D. Youn 대한기계학회 2022 대한기계학회 춘추학술대회 Vol.2022 No.11

Physics-informed Gaussian process probabilistic modeling for prognostics of degradation processes

Chen Jiang,Hyunhee Choi,Byeng D. Youn 대한기계학회 2022 대한기계학회 춘추학술대회 Vol.2022 No.11

Construction and validation of a seven-gene signature for predicting overall survival in patients with kidney renal clear cell carcinoma via an integrated bioinformatics analysis

Jiang Huiming,Chen Haibin,Chen Nanhui 한국통합생물학회 2020 Animal cells and systems Vol.24 No.3

Kidney renal clear cell carcinoma (KIRC) remains a significant challenge worldwide because of its poor prognosis and high mortality rate, and accurate prognostic gene signatures are urgently required for individual therapy. This study aimed to construct and validate a seven-gene signature for predicting overall survival (OS) in patients with KIRC. The mRNA expression profile and clinical data of patients with KIRC were obtained from The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC). Prognosis-associated genes were identified, and a prognostic gene signature was constructed. Then, the prognostic efficiency of the gene signature was assessed. The results obtained using data from the TCGA were validated using those from the ICGC and other online databases. Gene set enrichment analyses (GSEA) were performed to explore potential molecular mechanisms. A seven-gene signature (PODXL, SLC16A12, ZIC2, ATP2B3, KRT75, C20orf141, and CHGA) was constructed, and it was found to be effective in classifying KIRC patients into high- and low-risk groups, with significantly different survival based on the TCGA and ICGC validation data set. Cox regression analysis revealed that the seven-gene signature had an independent prognostic value. Then, we established a nomogram, including the seven-gene signature, which had a significant clinical net benefit. Interestingly, the seven-gene signature had a good performance in distinguishing KIRC from normal tissues. GSEA revealed that several oncological signatures and GO terms were enriched. This study developed a novel seven-gene signature and nomogram for predicting the OS of patients with KIRC, which may be helpful for clinicians in establishing individualized treatments.

Dexmedetomidine promotes the progression of hepatocellular carcinoma through hepatic stellate cell activation

Chen Peng,Luo Xiaojun,Dai Guanqi,Jiang Yuchuan,Luo Yue,Peng Shuang,Wang Hao,Xie Penghui,Qu Chen,Lin Wenyu,Hong Jian,Ning Xue,Li Aimin 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-

Dexmedetomidine (DEX) is an anesthetic that is widely used in the clinic, and it has been reported to exhibit paradoxical effects in the progression of multiple solid tumors. In this study, we sought to explore the mechanism by which DEX regulates hepatocellular carcinoma (HCC) progression underlying liver fibrosis. We determined the effects of DEX on tumor progression in an orthotopic HCC mouse model of fibrotic liver. A coculture system and a subcutaneous xenograft model involving coimplantation of mouse hepatoma cells (H22) and primary activated hepatic stellate cells (aHSCs) were used to study the effects of DEX on HCC progression. We found that in the preclinical mouse model of liver fibrosis, DEX treatment significantly shortened median survival time and promoted tumor growth, intrahepatic metastasis and pulmonary metastasis. The DEX receptor (ADRA2A) was mainly expressed in aHSCs but was barely detected in HCC cells. DEX dramatically reinforced HCC malignant behaviors in the presence of aHSCs in both the coculture system and the coimplantation mouse model, but DEX alone exerted no significant effects on the malignancy of HCC. Mechanistically, DEX induced IL-6 secretion from aHSCs and promoted HCC progression via STAT3 activation. Our findings provide evidence that the clinical application of DEX may cause undesirable side effects in HCC patients with liver fibrosis.

Load Forecasting Based on LSTM Neural Network and Applicable to Loads of “Replacement of Coal with Electricity”

Chen Zexi,Zhang Delong,Jiang Haoran,Wang Longze,Chen Yongcong,Xiao Yang,Liu Jinxin,Zhang Yan,Li Meicheng 대한전기학회 2021 Journal of Electrical Engineering & Technology Vol.16 No.5

With the complete implementation of the “Replacement of Coal with Electricity” policy, electric loads borne by urban power systems have achieved explosive growth. The traditional load forecasting method based on “similar days” only applies to the power systems with stable load levels and fails to show adequate accuracy. Therefore, a novel load forecasting approach based on long short-term memory (LSTM) was proposed in this paper. The structure of LSTM and the procedure are introduced firstly. The following factors have been fully considered in this model: time-series characteristics of electric loads; weather, temperature, and wind force. In addition, an experimental verification was performed for “Replacement of Coal with Electricity” data. The accuracy of load forecasting was elevated from 83.2 to 95%. The results indicate that the model promptly and accurately reveals the load capacity of grid power systems in the real application, which has proved instrumental to early warning and emergency management of power system faults. With the complete implementation of the “Replacement of Coal with Electricity” policy, electric loads borne by urban power systems have achieved explosive growth. The traditional load forecasting method based on “similar days” only applies to the power systems with stable load levels and fails to show adequate accuracy. Therefore, a novel load forecasting approach based on long short-term memory (LSTM) was proposed in this paper. The structure of LSTM and the procedure are introduced fi rstly. The following factors have been fully considered in this model: time-series characteristics of electric loads; weather, temperature, and wind force. In addition, an experimental verifi cation was performed for “Replacement of Coal with Electricity” data. The accuracy of load forecasting was elevated from 83.2 to 95%. The results indicate that the model promptly and accurately reveals the load capacity of grid power systems in the real application, which has proved instrumental to early warning and emergency management of power system faults.

Significant Association of Alpha-Methylacyl-CoA Racemase Gene Polymorphisms with Susceptibility to Prostate Cancer: a Meta-Analysis

Chen, Nan,Wang, Jia-Rong,Huang, Lin,Yang, Yang,Jiang, Ya-Mei,Guo, Xiao-Jiang,He, Ya-Zhou,Zhou, Yan-Hong Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.5

Background: Alpha-methylacyl-CoA racemase(AMACR) is thought to play key roles in diagnosis and prognosis of prostate cancer. However, studies of associations between AMACR gene polymorphisms and prostate cancer risk reported inconsistent results. Therefore, we conducted the present meta-analysis to clarify the link between AMACR gene polymorphisms and prostate cancer risk. Materials and Methods: A literature search was performed in PubMed, Embase, China National Knowledge Infrastructure (CNKI), Wanfang and Weipu databases. Odds ratios (ORs) and 95% confidence intervals (95%CIs) were calculated to assess the strength of any association between AMACR polymorphisms and prostate cancer risk. Subgroup analyses by ethnicity, source of controls, quality control and sample size were also conducted. Results: Five studies covering 3,313 cases and 3,676 controls on five polymorphisms (D175G, M9V, S201L, K277E and Q239H) were included in this meta-analysis. Significant associations were detected between prostate cancer and D175G (dominant model: OR=0.89, 95%CI=0.80-0.99, P=0.04) and M9V (dominant model: OR=0.87, 95%CI=0.78-0.97, P=0.01) polymorphisms as well as that in subgroup analyses. We also observed significant decreased prostate cancer risk in the dominant model (OR=0.90, 95%CI=0.81-0.99, P=0.04) for the S201L polymorphism. However, K277E and Q239H polymorphisms did not appear to be related to prostate cancer risk. Conclusions: The current meta-analysis indicated that D175G and M9V polymorphisms of the AMACR gene are related to prostate cancer. The S201L polymorphism might also be linked with prostate cancer risk to some extent. However, no association was observed between K277E or Q239H polymorphisms and susceptibility to prostate cancer.

Levels of Phenolic Compounds, Antioxidant Capacity, and Microbial Counts of Fresh-cut Onions after Treatment with a Combination of Nisin and Citric Acid

Chen Chen,Wenzhong Hu,Ruidong Zhang,Aili Jiang,Yu Zou 한국원예학회 2016 Horticulture, Environment, and Biotechnology Vol.57 No.3

Nisin and citric acid are generally recognized as safe (GRAS) for using as a food ingredient and often used to control the microbial contamination of fresh-cut fruits and vegetables. However, their effects on phenolics and antioxidants remain unclear. Here we used 50 μg·mL-1 nisin combination with 1% (w/v) citric acid (N+C) to wash fresh-cut onions and the phenolic compounds, antioxidant capacity, microbial load, slice color were evaluated during the storage period. N+C treatment decreased the total polyphenols (TP) and quercetin contents (p < 0.05), as compared to the control at day 0, but did not affect phenolic acids and antioxidant capacity (ABTS, DPPH, and FRAP assays). During storage, antioxidant capacity, TP, and flavonoid contents increased. Storage had mixed effects on the phenolic acids (caffeic acid, vanillic acid, and ferulic acid increased, chlorogenic acid remained fairly stable). N+C treatment reduced the microbial counts and total viable counts (TVC) of fresh-cut onions below the detection limit until 5 days of storage. After 15 days storage, TVC of N+C treated samples remained significantly (p < 0.05) lower than controls. In addition, N+C treatment led to better color retention during storage. Therefore, N+C might be used as a safe preservative for fresh-cut onions.