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Hee Ji Moon,Soyoung Choi,Jiyeun Park,Sieun Kim,Nahyun Lee,Yejin Choi,Ji Young Shin,Jung-Eun Kim,Hokyoung Son 한국당과학회 2021 한국당과학회 학술대회 Vol.2021 No.01
Glycosylation contributes to various molecular and cellular functions such as protein folding, localization, secretion, cell signaling, and communication by transferring glycans to proteins or lipids. Glycoproteins are expected to play essential roles for various biological processes including pathogenicity and reproductions in fungi; however, little is known about glycosylation process in filamentous fungi, especially in plant pathogenic fungi. The plant pathogenic fungus Fusarium graminearum causes a devastating disease called Fusarium head blight (FHB) on cereal crops. In addition to grain yield losses, infection of this pathogen causes severe contamination of mycotoxins, which are harmful to human and animal health. To understand molecular mechanisms underlying development and pathogenesis of this fungus, putative 78 N or O-glycosylation-related gene (GLY) knockout mutant library is being generated and we have successfully obtained 53 deletion mutations till now. Phenotypic changes, such as vegetative growth, sexual and asexual reproduction, and virulence of the mutants are being analyzed. Some mutants showed severe phenotypic defects suggesting that individual GLY gene plays important roles in this fungus. The ongoing works are focusing on the elucidation of the potential roles of GLY genes in diverse biological processes, such as fungal virulence, mycotoxin production and we are also analyzing glycan structure of glycoproteins in F. graminearum. This study is the first comprehensive functional analysis of GLY gene in plant pathogenic fungi, and it will be the fungal glycobiome study initiative.
Haeeun Shin(Haeeun Shin),Joon Ki Min(Joon Ki Min),Na Rae Kim(Na Rae Kim),Kyoung Yul Seo(Kyoung Yul Seo),Hee Seung Chin(Hee Seung Chin),Soyoung Lee(Soyoung Lee),Ji Won Jung(Ji Won Jung) 대한안과학회 2023 Korean Journal of Ophthalmology Vol.37 No.1
Purpose: Human corneal endothelial progenitor cells (HCEPs), which has been selectively isolated and differentiated into human corneal endothelial cells (HCECs), are crucial for repairing corneal endothelial damage. In this study, we evaluated the roles of a Rho-assisted kinase (ROCK) inhibitor, Y-27632, on the isolation and expansion of HCEPs, and assessed the in vitro effects of different concentrations of Y-27632 on the differentiated HCEPs. Methods: HCEPs were isolated and expanded in a medium with and without 10μM Y-27632, and then differentiated into HCECs in a medium with fetal bovine serum. The characteristics of HCEPs and differentiated HCEPs were confirmed by immunofluorescence staining. The proliferation, viability, morphology, and wound-healing ability of differentiated HCEPs were assessed in the presence of different concentrations of Y-27632. Results: Y-27632 enabled the isolation and expansion of HCEPs from the corneal endothelium. The differentiated HCEPs showed an optimal increase in proliferation and survival in the presence of 10μM Y-27632. As the concentration of Y-27632 increased, differentiated HCEPs became elongated, and actin filaments were redistributed to the periphery of cells. Y-27632 also caused a concentration-dependent enhancement in the wound-healing ability of differentiated HCEPs. Conclusions: Y-27632 enabled the isolation and expansion of HCEPs. It also enhanced the proliferation, viability, and migration of differentiated HCEPs.
Ji Eun Hwang,Su-Hyun Kim,Soyoung Park 순천향대학교 순천향의학연구소 2019 Journal of Soonchunhyang Medical Science Vol.25 No.2
Myelin oligodendrocyte glycoprotein (MOG) antibody-associated diseases are reported to have distinct pathogenic mechanisms, clinical courses, and therapeutic implications in comparison to other acquired demyelinating syndromes. Here, we report the case of a 6-year-old Korean girl with recurrent steroid-responsive optic neuritis (ON) and an episode of acute disseminated encephalomyelitis (ADEM) with a high MOG antibody titer. Initially, she suffered from left ON with subclinical brain lesions including magnetic resonance imaging hyperintensities in the bilateral frontal subcortical white matter, the left thalamus, and the right cerebral peduncle. Her vision improved dramatically after steroid therapy; however, 3 months after the 1st episode, she developed acute encephalopathy with extensive newly developed ADEM-like lesions and resolution of previous hyperintensities. Despite regular interferon β-1a injection after steroid tapering, she developed a 3rd episode presenting as a right ON. Throughout these three episodes, serological tests associated with autoimmune diseases, viral markers, anti-aquaporin-4 antibodies, and cerebrospinal fluid oligoclonal bands were negative, with the immunoglobulin G index in the normal range. After the 3rd episode, anti-MOG antibody testing was performed and higher titer was detected. Testing for anti-MOG antibodies is highly recommended for relapsing inflammatory demyelinating central nervous system diseases in pediatric patients, in order to define early an adequate long-term treatment.
SoYoung Kim,Young-Kwan Kim,Hye-Yeon Shim,Ji-Hyun Seo,Jun-Ho Kim 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.49 No.3
We fabricated white organic light-emitting diodes (WOLEDs) with three emissive layers consisting of a phosphorescent and two fluorescent materials. The fabricated device structure was ITO / NPB(40 nm) / 1 % Ir(ppy)3:CBP(5 nm) / DPVBi(10 nm) /BCP (3 nm)/ 1 % DCM1 : CBP(5 nm) / BCP(10 nm) / Alq3(30 nm) / Liq(2 nm) / Al. In this device, the thin BCP between the DPVBi and the 2 % DCM1 played the role of retarding the hole flow from the DPVBi layer to the 2 % DCM1 : CBP layer, resulting in a white emission due to the strong blue emission of DPVBi. The device showed an improved white balance with Commission Internationale d’Eclairage (CIE) coordinates of (0.278, 0.371) and a reduced maximum luminance of 3,440 cd/m2 while the device without the thin BCP layer showed CIE coordinates of (0.311, 0.417) and a maximum luminance of 4,510 cd/m2.