<i>Pedobacter pituitosus</i> sp. nov., isolated from a waterfall

Chun, Jeesun,Kang, Ji Young,Jahng, Kwang Yeop International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.11

<P>A taxonomic study was carried out on a novel bacterial strain, designated MIC2002<SUP>T</SUP>, which was isolated from Wibong falls in Korea. Cells were Gram-stain-negative, aerobic, non-motile and rods, 0.3–0.5 µm wide and 4.0–5.0 µm long. The optimum temperature and pH range for growth were 25 °C and pH 6.5–7.0, respectively. Catalase and oxidase activities were positive. Flexirubin pigments were not produced. Comparison of the 16S rRNA gene sequences indicated that the isolate belonged to the genus <I>Pedobacter</I>, with <I>Pedobacter daechungensis</I> as its closest relative, with a similarity of 94.4 %. It contained iso-C<SUB>15 : 0</SUB>, anteiso-C<SUB>15 : 0</SUB>, C<SUB>16 : 0</SUB>, summed feature 3 (C<SUB>16 : 1</SUB>ω6<I>c</I> and/or C<SUB>16 : 1</SUB>ω7<I>c</I>) and iso-C<SUB>17 : 0</SUB> 3-OH as the major fatty acids and menaquinone MK-7 as isoprenoid quinone. The polar lipid profile of strain MIC2002<SUP>T</SUP> revealed the presence of phosphatidylethanolamine and an unknown lipid. The DNA G+C content of the strain was 34.7 mol%. On the basis of the evidences presented, it was concluded that strain MIC2002<SUP>T</SUP> represents a novel species of the genus <I>Pedobacter</I> within the family <I>Sphingobacteriaceae</I>, for which the name <I>Pedobacter</I> <I>pituitosus</I> sp. nov. is proposed. The type strain is MIC2002<SUP>T</SUP> ( = KACC 17064<SUP>T</SUP> = JCM 18729<SUP>T</SUP>).</P>

Comparative Transcriptomic Analysis of MAPK-Mediated Regulation of Sectorization in Cryphonectria parasitica

Chun, Jeesun,So, Kum-Kang,Ko, Yo-Han,Kim, Jung-Mi,Kim, Dae-Hyuk Korean Society for Molecular and Cellular Biology 2019 Molecules and cells Vol.42 No.4

Fungal sectorization is a complex trait that is still not fully understood. The unique phenotypic changes in sporadic sectorization in mutants of CpBck1, a mitogen-activated protein kinase kinase kinase (MAPKKK) gene, and CpSlt2, a mitogen-activated protein kinase (MAPK) gene, in the cell wall integrity pathway of the chestnut blight fungus Cryphonectria parasitica have been previously studied. Although several environmental and physiological factors cause this sectoring phenotype, genetic variants can also impact this complex morphogenesis. Therefore, RNA sequencing analysis was employed to identify candidate genes associated with sectorization traits and understand the genetic mechanism of this phenotype. Transcriptomic analysis of CpBck1 and CpSlt2 mutants and their sectored progeny strains revealed a number of differentially expressed genes (DEGs) related to various cellular processes. Approximately 70% of DEGs were common between the wild-type and each of CpBck1 and CpSlt2 mutants, indicating that CpBck1 and CpSlt2 are components of the same MAPK pathway, but each component governs specific sets of genes. Functional description of the DEGs between the parental mutants and their sectored progenies revealed several key pathways, including the biosynthesis of secondary metabolites, translation, amino acid metabolism, and carbohydrate metabolism; among these, pathways for secondary metabolism and translation appeared to be the most common pathway. The results of this comparative study provide a better understanding of the genetic regulation of sector formation and suggest that complex several regulatory pathways result in interplays between secondary metabolites and morphogenesis.

Report on 14 unrecorded bacterial species in Korea that belong to the phyla Bacteroidetes and Deinococcus-Thermus

Jeesun Chun,Jin-Woo Bae,CHANG-JUN CHA,조장천,JONGSIK CHUN,임완택,Che Ok Jeon,KISEONG JOH,김승범,성치남,Jung-Hoon Yoon,Kwang-Yeop Jahng 국립생물자원관 2015 Journal of species research Vol.4 No.2

As a result of the research project ‘Survey and excavation of Korean indigenous species’ to secure unrecorded species in Korea, a total of 14 unreported bacterial strains assigned to the phyla Deinococcus- Thermus and Bacteroidetes were isolated from various environmental habitats all around Korea. On the basis of 16S rRNA gene sequence similarity and affiliation to the clade with the closest species, it was concluded that the isolates represent deep groups of the phyla Deinococcus-Thermus and Bacteroidetes. There have been no records about these 14 unreported species in Korea; therefore 2 species of 2 genera in the class Deinococci within the phylum Deinococcus-Thermus, and 11 species of 9 genera in the class Flavobacteriia and one species in one genus in the class Sphigobacteriia within the phylum Bacteroidetes are described as unreported species found in Korea. Gram staining reaction, morphological and other biochemical characteristics are described in the species description section.

Report on 14 unrecorded bacterial species in Korea that belong to the phyla Bacteroidetes and Deinococcus-Thermus

Chun, Jeesun,Bae, Jin-Woo,Cha, Chang-Jun,Cho, Jang-Cheon,Chun, Jongsik,Im, Wan-Taek,Jeon, Che Ok,Joh, Kiseong,Kim, Seung Bum,Seong, Chi Nam,Yoon, Jung-Hoon,Jahng, Kwang Yeop The National Institute of Biological Resources 2015 Journal of species research Vol.4 No.2

As a result of the research project 'Survey and excavation of Korean indigenous species' to secure unrecorded species in Korea, a total of 14 unreported bacterial strains assigned to the phyla Deinococcus-Thermus and Bacteroidetes were isolated from various environmental habitats all around Korea. On the basis of 16S rRNA gene sequence similarity and affiliation to the clade with the closest species, it was concluded that the isolates represent deep groups of the phyla Deinococcus-Thermus and Bacteroidetes. There have been no records about these 14 unreported species in Korea; therefore 2 species of 2 genera in the class Deinococci within the phylum Deinococcus-Thermus, and 11 species of 9 genera in the class Flavobacteriia and one species in one genus in the class Sphigobacteriia within the phylum Bacteroidetes are described as unreported species found in Korea. Gram staining reaction, morphological and other biochemical characteristics are described in the species description section.

<i>Psychroflexus salarius</i> sp. nov., isolated from Gomso salt pan

Chun, Jeesun,Kang, Ji Young,Jahng, Kwang Yeop International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.10

<P>A taxonomic study was carried out on a novel bacterial strain, designated MIC1008<SUP>T</SUP>, which was isolated from the Gomso salt pan, located in Buan County, Korea. Cells were Gram-stain-negative, aerobic, non-motile and moderately halophilic rods, 0.2–0.4 µm wide and 0.7–1.2 µm long. The optimum temperature and pH for growth were 25 °C and pH 6.5–8.5, respectively. Catalase and oxidase activities were positive. Carotenoid pigments were produced. Comparison of 16S rRNA gene sequences indicated that the isolate belonged to the genus <I>Psychroflexus</I>, with <I>Psychroflexus halocasei</I> WCC 4520<SUP>T</SUP> as its closest relative, with a 16S rRNA gene sequence similarity of 95.4 %. The isolate contained iso-C<SUB>15 : 0</SUB>, iso-C<SUB>15 : 1</SUB> G, iso-C<SUB>17 : 0</SUB> 3-OH, anteiso-C<SUB>15 : 0</SUB> and iso-C<SUB>15 : 0</SUB> 3-OH as the major fatty acids and menaquinone MK-6 as the isoprenoid quinone. The major polar lipid profile of strain MIC1008<SUP>T</SUP> revealed the presence of phosphatidylethanolamine, an unidentified aminolipid and two unidentified lipids. The DNA G+C content of the isolate was 32.9 mol%. On the basis of the evidences presented, it is concluded that strain MIC1008<SUP>T</SUP> represents a novel species of the genus <I>Psychroflexus</I> within the family <I>Flavobacteriaceae</I>, for which the name <I>Psychroflexus</I> http://dx.doi.org/10.1601/nm.8182 <I>salarius</I> sp. nov. is proposed. The type strain is MIC1008<SUP>T</SUP> ( = KACC 17063<SUP>T</SUP> = DSM 25661<SUP>T</SUP>).</P>

시선과 공적 자의식이 인상형성에 미치는 효과

조지선(Jeesun Cho),신고은(Go Eun Shin),전우영(Woo Young Chun) 충남대학교 사회과학연구소 2017 사회과학연구 Vol.28 No.2

The present research addresses the effects of direct gaze and public self-consciousness on impression formation. Specifically, we investigated whether direct gaze changes the extent to which participants perceive the target persons as warm upon seeing their face. In the experimental condition, a series of staring eye images were presented to participants whereas in the controlled condition, a series of closed eye images were presented. We also explored how public self-consciousness influences the impression of warmth. Results showed that regardless of individual differences in public self-consciousness, participants uniformly perceived the target persons as rather cold when exposed to staring eyes. On the contrary, when closed eye images were presented, the participants with low public self-consciousness formed warmer impression of the targets in comparison to those with high public self-consciousness. The implications of these findings for excessive exposures to staring eyes are discussed.

The analysis of enzyme activities between mycovirus-cured Trichoderma harzianum M6 and mycovirus-infected T. harzianum M6

Myeongjin Jo,Jeesun Chun,Mi-Ra Jin,Song Hee Lee 한국농약과학회 2018 한국농약과학회 학술발표대회 논문집 Vol.2018 No.10

Antimicrobial and Antitumor Photodynamic Effects of Phleichrome from the Phytopathogenic Fungus Cladosporium Phlei

( Kum-kang So ),( Jeesun Chun ),( Dae-hyuk Kim ) 한국균학회 2018 Mycobiology Vol.46 No.4

Fungal perylenequinones have photodynamic activity and are promising photosensitizers for photodynamic therapy (PDT). Here, we investigated the bactericidal and antitumor activities of phleichrome from the fungal perylenequinone family in vitro. Photodynamic bactericidal activity of phleichrome was analyzed by agar-well diffusion method under dark and illuminated conditions. The photodynamic antitumor activity of phleichrome was analyzed in MCF-7, HeLa, SW480, and HepG2 human cancer cell lines using in vitro cytotoxicity assays. Photodynamic bactericidal activities against Gram-negative and Gram-positive bacteria were species-specific. Antitumor activity against all tumor cell lines increased under the illuminated condition. Depending on the results of the analyses, Phleichrome has potential for further drug development related to its antibacterial and antitumor activities.

A Report on Gene Design for Escherichia coli Expression

Ngoc-Luong NGUYEN,Jeesun CHUN,Yo-Han KO,Byeonghak NA,Dae-Hyuk KIM 한국생물공학회 2020 한국생물공학회 학술대회 Vol.2020 No.10

<i>Nibrella saemangeumensis</i> gen. nov., sp. nov. and <i>Nibrella viscosa</i> sp. nov., novel members of the family <i>Cytophagaceae</i>, isolated from seawater

Kang, Ji Young,Chun, Jeesun,Choi, Ahyoung,Cho, Jang-Cheon,Jahng, Kwang Yeop International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.12

<P>Two strains, designated GCR0103<SUP>T</SUP> and GYR3121<SUP>T</SUP>, were isolated from seawater of the Saemangeum Embankment in Jeollabuk-do, Korea. The cells of the two strains were Gram-reaction-negative and non-motile, and formed multicellular filaments. The colonies of the two strains were pink-pigmented and able to grow at 15–37 °C (optimum 25 °C) on R2A and NA medium. Strains GCR0103<SUP>T</SUP> and GYR3121<SUP>T</SUP> grew at pH 6.5–10 (optimum pH 7.5) and pH 5.5–9.5 (optimum pH 7.5), and within NaCl ranges of 0–0.4 % and 0–1 %, respectively. The polar lipid profiles of the two strains contained phosphatidylethanolamine, five unknown aminolipids, an unknown phospholipid and four or five unknown lipids. The DNA G+C contents of strains GCR0103<SUP>T</SUP> and GYR3121<SUP>T</SUP> were 56.0 and 54.5 mol%, respectively. The respiratory quinone detected in both strains was MK-7. The 16S rRNA gene sequence similarity between GCR0103<SUP>T</SUP> and GYR3121<SUP>T</SUP> was 95.5 %. The 16S rRNA gene sequence similarities of the two strains to closely related reference strains were less than 89 %. Phylogenetic analysis based on 16S rRNA genes showed that GCR0103<SUP>T</SUP> and GYR3121<SUP>T</SUP> formed a distinct phyletic line in the family <I>Cytophagaceae</I>. On the basis of the phenotypic, chemotaxonomic and phylogenetic properties, strains GCR0103<SUP>T</SUP> and GYR3121<SUP>T</SUP> represent two novel species in a new genus within the family <I>Cytophagaceae</I>, for which the names <I>Nibrella saemangeumensis</I> gen. nov., sp. nov. and <I>Nibrella viscosa</I> sp. nov. are proposed. The type strain of <I>Nibrella saemangeumensis</I> is GCR0103<SUP>T</SUP> ( = KACC 16453<SUP>T</SUP> = JCM 17927<SUP>T</SUP>) and the type strain of <I>Nibrella viscosa</I> is GYR3121<SUP>T</SUP> ( = KACC 16447<SUP>T</SUP> = JCM 17925<SUP>T</SUP>).</P>