콩 이소플라본이 성장기 쥐의 골대사에 관한 연구

김영경,심재영,이헌옥,양승오,엄애선 한국콩연구회 2003 韓國콩硏究會誌 Vol.20 No.2

Soybean has been consumed in Asia as a lot of soyfood products. Soy isoflavones, particularly genistein. have preventive effect on breast and prostate cancer, atherosclerosis and osteoporosis. To investigate if the influence of soy isoflavones on bone metabolism we used 3-week- old female Wister Rat ovariectomized. All the rats were divided into sham (SH) , ovariectomized (OVX) , OVX- 17β-estradiol (10 ㎎/㎏ b.w.), OVX-genistein (5 ㎎/㎏ b.w.). They were freely fed foods and drinking water for 8 weeks. Results show that body weight had significantly decreased in SH group, compared to OVX groups. The serum alkaline phosphatase (ALP) was increased in SH, OVX-GEN and OVX-ES groups but not significant. Creatinine and hydroxyproline level lowered in SH group but not significant. Femur, feces, serum and urinary Ca level was significant and femur, serum P level did not differ. Bone mineral density (BMD) decreased OVX group but increased slightly isoflavone group. It suggests that estrogen may affect bone mineralization in growing rats and soy isoflavones may involve prevention of bone loss. More studies are needed to identify the mechanism of soy isoflavones and bone formation.

식품 위해 요소중점 관리 기준에 대한 서울지역 사업체 급식관리자의 위생관리평가

이헌옥,심재영,김영경,조민호,최호순,엄애선 한국조리과학회 2001 한국식품조리과학회지 Vol.17 No.6

Hazard Analysis Critical Control Point(HACCP) is becoming an important component of food safety worldwide. The aim of this study was to investigate comprehensively the education and knowledge level of food service managers on HACCP as well as applying HACCP system to industrial foodservice. Total 247 foodservice managers participated in the survey and 159 responses were used for analysis. The results were as follows; 1) 89% of foodservice managers were educated about HACCP, and 40.9% felt they did fully understand HACCP and 47.8% did half. 2) The score for the implementation of HACCP was in the order of apparatus and facility sanitation, personal sanitation, and time-temperature/etc. sanitation. 3) Foodservice managers who were taught HACCP kept a deep attention to food and personal sanitation, compared with those without education(p<0.05). However, the education time on HACCP affected conducting safety management. The results suggest that education and understanding of HACCP are positively related, and understanding of HACCP has a positive influence on conducting safety management.

열처리에 따른 Cd₁-xZnxS/In 박막의 광학적 및 전기적 특성 분석

송우창,이경호,심상흥,이상교,심재선,박종국 三陟大學校 2002 論文集 Vol.35 No.-

In this thesis, effects of the thickness of indium films and the annealing temperature on optical and electrical properties of chemically deposited CdZnS films for the efficiency of the Cd1-xZnxS/CdTesolar cells were investigated. To improve the electrical conductivity of Cd1-xZnxs films, thin indium films(10-4Onm thickness)were evaporated on the suface of Cd1-rZnxs films. Experimental parameters such as indium film thickness, Zn mole fraction, and the annealing temperature in terms of structural and electrical properties of Cd1-xZnxs films were investigated. The diffusion process was carried out by heating the sample in air at 150-550℃ for 20 minute As the indium film thickness is increased, the resistivity decreases and the lowest value(0.3 O -cm)is obtained for Cd1-xZnxs films annealed at 450℃ with 40nm of the thickness.

Molecular Cloning of Glucose-Regulated Protein 78 Gene in HSP70 Family and its Expression in Reation to Feeding Mechanism of Plodia interpunctella

Jae-Kyoung Shim,Tin Tin Aye,Kyeong-Yeoll Lee 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

A glucose-regulated protein 78 (grp78) gene, which is belongs to a heat shock cognate 70 (hsc70) subfamily, was cloned from Indian meal moth, Plodia interpunctella. Its full length cDNA was 2679 bp and contains a 1980 bp open reading frame. The translated amino acid sequence consists of 660 residues with a calculated molecular mass of 72,975 Da and an isoelectronic point (pI) of 5.27. It contains several highly conserved functional motifs of the Hsp70 family and, particularly, C-terminal motif of KDEL that is characteristic for endoplasmic reticulum (ER) hsc70. Its deduced amino acid sequence shows a high identity (83-94%) with Hsc70s of other insects and grouped with Hsc70-3 among 5 Hsc70 members of Drosophila melanogaster. During development the grp78 transcript level was high in egg, feeding larval and adult stages but low in molting and wandering larval and pupal stages. Particularly its level was higher in the gut than integument and fat body of fifth instars. Furthermore its level was greatly decreased when fifth instar larvae were starved for 48 hrs but recovered at 3-6 hrs after re-fed diet. Our data suggests that grp78 is a member of hsc70 gene that belongs to ER and may have a role for energy metabolism at cellular level.

DIFFERENT INFECTIVITY OF HEPATITIS B VIRUS BETWEEN PRIMARY HUMAN ADULT AND FETAL HEPATOCYTES

Jae Kyoung Shim,Bong Seok Kang,Young Choon Lee,Tae Ho Chung,Cheorl Ho Kim 생화학분자생물학회 1997 생화학분자생물학회 춘계학술발표논문집 Vol.- No.-

HCV, Alcoholic : PE-091 ; Gender difference in liver diseases: is iron deficiency in women correlated with lower risk for liver diseases?

( Jae Jun Shim ),( Hoe Hoon Chung ),( Min Kyoung Kim ),( Chang Kyun Lee ),( Jae Young Jang ),( Byung Ho Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

Background/Aim: Accumulated iron can injure hepatocytes by oxidative stress. Due to regular menstruation, women had lower body iron than men. However, it is not clear whether gender difference in liver diseases is correlated with iron store. Methods: We analyzed data (aged 20 to 65 years) from The Korea National Health and Nutrition Examination Survey in 2010. In order to diminish confounding factors, only women were included. Iron deficient women (transferrin saturation < 15%) were compared with those who had iron overload (transferrin saturation > 55%). Liver injury was defined as elevation of alanine aminotransferase (ALT) over 40 U/L. Results: Of 2,703 females, 310 (11.5%) had iron deficiency and 172 (6.4%) had iron overload. There were no differences in age, height, body weight, and prevalence of hypertension and diabetes between two groups (p > 0.05). The iron-deficient group had lower levels of gamma glutamyl transpeptidase, hemoglobin, and ferritin than the iron-overload group (p < 0.001, respectively). However, ALT level was not increased in subjects with iron overload (14.5±0.6 U/L in iron deficiency vs. 17.8±1.4 U/L in iron overload, p = 0.09). The proportion of iron deficiency did not differ between liver injury group (6.5%) and normal ALT group (11.6%) (p = 0.180). Iron overload showed similar proportion in two groups (8.7% in liver injury group vs. 6.3% in normal) (p = 0.379). Liver injury was associated with older age, hypertension, diabetes, dyslipidemia, obesity, metabolic syndrome, and hyperferritinemia (> 200 ng/mL) (p<0.001, respectively). Conclusions: In general female population, body iron is not associated with liver injury. Iron deficiency does not decrease liver injury. These findings suggest lower body iron may not be associated with lower risk for liver diseases in women.

Differential Expression of N-Acetylglucosaminyltransferase-3 and-V Activities in Human Hepatoma Cells and Colon Cancer Cells : Implication of Cancer Specific-Glycoantigen Synthesis in Malignant Transformation 악성화과정중 암특이당쇄항원의 의미와 억제

Shim, Jae Kyoung,Park, Chun,Song, Eun Young,Lee, Young Choon,Chung, Tae Wha,Kim, Cheorl Ho 한국키틴키토산학회 1997 한국키틴키토산학회지 Vol.2 No.3

본 연구그룹은 정상세포가 형질전환되어가는 과정에서 당쇄항원생합성을 하는 효소가 N-아세틸글루코사민전이효소임을 밝히고 이들의 분자세포생물학적인 연구와 Transgenic mouse 개발을 통한 암예방,치료,조기진단에 대한 연구를 수행하여 왔다. 본 논문에서는 사람 간암 및 대장암세포에서 암당쇄항원합성효소 N-acetylglucosaminyltransferase (GlcNAc-transferase)- Ⅲ 과 V활성의 발현정도를 정상세포들과 비교하였다. 정상세포들의 활성은 매우 낮았으며, 간암세포에서 GlcNAc-transferase-Ⅲ활성이GlcNAc-transferase-V보다 높았으나, 대장암세포에서는 훨씬 낮게 발현되었다. 기질로서 GlcN,GlcN-biant-PA와 UDP-GlcNAc를 사용하였을 때, 각각의 효소들은 간암과 대장암세포에서 이들의 암전이능력에 의존하여 각각 다른 반응특성을 나타내었다. 이러한 결과들은 GlcNAc-transferase-Ⅲ 와 V유전자발현(mRNA)을 위한 RT-PCR결과에서도 동일하였다. 이러한 암세포에서 암당쇄항원의 생합성에 관여하는 N-acetylglucosamine 전이효소의 특이적인 발현과 악성화과정중 암특이당쇄항원과의 관계를 분자세포생물학적 및 기능성식품학적인 측면에서 토론하였다. UDP-N-Aacetylglucosamine:α-6-D-mannoside β-1,6N-acetylglucosaminyltransferase-Ⅲ (GlcNAc-transferase-Ⅲ) and UDP-N-Aacetylglucosamine : α-6-D-mannoside β-1,6N-acetylglucosaminyltransferase - V (GlcNAc -transferase - V )activities were determined in human hepatoma cell lines of Hep3B and HepG2, and also compared with those of normal liver tissues and primary hepatocytes. GlcNAc-transferase-Ⅲ activities were higher than those of GlcNAc-transferase-V in hepatic carcinomca cells. In contrast, the two enzyme activities were assayed in highly metastatic colon cancer cells, GlcNAc-transferase-V activities were much higher than those of GlcNAc-transferase-Ⅲ . When GlcN,GlcN-biant-PA and UDP-GlcNAc were used as substrates, the enzymes displayed different kinetic properties between hepatic and colon cancer cells, depending on their metastatic potentials. Normal cells of two origins are characterized by a very low level of GlcNAc-transferase- Ⅲ and -V activities, whereas hepatoma and colon cancer cells cotained high activities. These data were supported by reverse transcription-polymerase chain reaction results, showing that expression of the GlcNAc-transferase-Ⅲ and V mRNAs increased in proportion to the enzymatic activities. Although the mechanism underlying the induction of this enzymes is unknown, lectin blot analysis showed that oligosaccharides in many glycopnteins were observed in cancer cells. Thus, this is the first demonstration of GlcNAc-transferase-Ⅲ and V activities in human hepatoma and colon cell lines. Molecular aspects of two GlcNAc-transferases in tumorigenesis and metastasis will be extensively discussed.

HCV : PE-091 ; Gender difference in liver diseases: is iron deficiency in women correlated with lower risk for liver diseases?

( Jae Jun Shim ),( Hoe Hoon Chung ),( Min Kyoung Kim ),( Chang Kyun Lee ),( Jae Young Jang ),( Byung Ho Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1

Background/Aim: Accumulated iron can injure hepatocytes by oxidative stress. Due to regular menstruation, women had lower body iron than men. However, it is not clear whether gender difference in liver diseases is correlated with iron store. Methods: We analyzed data (aged 20 to 65 years) from The Korea National Health and Nutrition Examination Survey in 2010. In order to diminish confounding factors, only women were included. Iron deficient women (transferrin saturation < 15%) were compared with those who had iron overload (transferrin saturation > 55%). Liver injury was defined as elevation of alanine aminotransferase (ALT) over 40 U/L. Results: Of 2,703 females, 310 (11.5%) had iron deficiency and 172 (6.4%) had iron overload. There were no differences in age, height, body weight, and prevalence of hypertension anddiabetes between two groups (p > 0.05). The iron-deficient group had lower levels of gamma glutamyl transpeptidase, hemoglobin, and ferritin than the iron-overload group (p < 0.001, respectively). However, ALT level was not increased in subjects with iron overload (14.5±0.6 U/L in iron deficiency vs. 17.8±1.4 U/L in iron overload, p = 0.09). The proportion of iron deficiency did not differ between liver injury group (6.5%) and normal ALT group (11.6%) (p = 0.180). Iron overload showed similar proportion in two groups (8.7% in liver injury group vs. 6.3% in normal) (p = 0.379). Liver injury was associated with older age, hypertension, diabetes, dyslipidemia, obesity, metabolic syndrome, and hyperferritinemia (> 200 ng/mL) (p<0.001, respectively). Conclusions: In general female population, body iron is not associated with liver injury. Iron deficiency does not decrease liver injury. These findings suggest lower body iron may not be associated with lower risk for liver diseases in women.

Evidence of diapause initiation of the ussur brown katydid, Paratlanticus ussuriensis, eggs in relation to temperature

Jae-Kyoung Shim,Woo-Geun Song,Hea-son Bang,Kyoeong-Yeoll Lee 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10

During the last several years, the population of the ussur brown katydid, Paratlanticus ussuriensis has been highly increased at mountain-side orchards in Youngdong province in Korea. Nymphs and adults emerged one time per year from early May to August and cause a serious demage on the crop production. Adults oviposit eggs from July to September under the ground and eggs overwinter at diapause stage. However, the diapause duration P. ussuriensis is known to either one year or prolonged to additional years. The environmental cues, such as temperature, could be a critical to determine the diapause duration but the precise diapause mechanism of P. ussuriensis eggs is unclear. We demonstrated to determine the stage of diapause initiation during embryogenesis. Newly oviposited eggs were incubated for 60 days at two temperature conditions (20℃and 30℃). When the eggs were incubated at 20℃ those weights were not changed, but when they incubated at 30℃ those were increased from 15-days-old. Total RNA contents of eggs incubated at 30℃ were highly increased from the 15-days-old when they compared with those of eggs incubated at 20℃. In addition, total DNA contents of eggs were greatly higher when they incubated at 30℃. Our results suggest that early embryonic development was sensitive to high temperature and it was stimulated at 15-days-old. To determine the temperature response of embryonic development at molecular level we identified heat shock protein 70 (hsp70) gene of P. ussuriensis. Further analysis would conduct the gene expression rate of diapausing eggs on different temperature conditions by using real time RT-PCR technique and determine the molecular characteristics of P. ussuriensis egg diapause.

Effects of oral ingestion of dsRNA of the sweetpotato whitefly, Bemisia tabaci

Jae-Kyoung Shim,Duck-Oung Jung,Bong-Gi Choi,Kyeong-Yeoll Lee 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.04

The sweetpotato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae) is a species complex, including at least 24 biotypes which express different ecological and physiological characteristics. B. tabaci is one of the most serious pests in the horticultural crops in worldwide level. B. tabaci infests over 600 plant species and also indirectly damages plants by the honeydew excretion. Moreover, whiteflies transmit more than 100 plant viruses, especially begomoviruses. Tomato yellow leaf curl virus (TYLCV) which infested severely in tomato cultivars is transmitted by the vector insect, B. tabaci. Here, we demonstrated whether gene expression of B. tabaci is regulated by the oral ingestion of dsRNA. Double strand RNA (dsRNA) of heat shock protein 70 gene (hsp70) was produced from cDNA by using the specific primer. Whitefly adults were allowed to ingest sugar solution containing dsRNA for 3 hr in the two-layered parafilm feeding tube. Quantitative realtime PCR showed that whiteflies which ingest hsp70 dsRNA completely knockdown its transcript expression. Whiteflies ingestded dsRNA increased mortality by heat shock but not by cold shock. Further research will focus the role of hsp70 in various environmental stresses against insects.