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Kim, J.D.,Hyun, Y.,Sohn, K.S.,Kim, T.J.,Woo, H.J.,Han, In K. Asian Australasian Association of Animal Productio 2001 Animal Bioscience Vol.14 No.3
An experiment was conducted to determine the optimal inclusion ratio of spray dried plasma protein (SDPP) and dried porcine solubles (DPS) for maximizing growth and improving immunity in weaned pigs. One hundred-fifty male (barrow) pigs were allotted in a completely randomized block design. Treatments were as follows: 1) control (6% SDPP), 2) S6D6 (6% SDPP+6% DPS), 3) S6D3 (6% SDPP+3% DPS), 4) S3D6 (3% SDPP+6% DPS) and 5) S3D3 (3% SDPP+3% DPS). Each treatment has 6 replicates with 5 pigs per replicate. Average daily gain (ADG) and average daily feed intake (ADFI) were highest, but not significantly different when pigs were fed a diet contained 6% SDPP and DPS from d 0 to 7 after weaning. Pigs fed the S6D3 diet showed better weight gain and feed intake than other treatments, especially compared with pigs fed S3D6 diet (p<0.05) from d 8 to 21 after weaning. For the overall experimental period, pigs fed the S6D3 diet showed the best improvement in ADG and ADFI. The digestibilities of dry matter (DM) and crude protein (CP) were higher in pigs fed the S6D6 diet than other diets from d 0 to 7 after weaning. However, pigs fed S6D3 diet showed higher DM, CP and essential amino acids (except methionine and arginine) digestibilities than pigs fed other diets from d 8 to 21 after weaning, although there was no significant difference. From d 8 to 21 after weaning, threonine, valine, isoleucine and leucine digestibilites were higher in S6D6 group, and phenyalanine, histidine, lysine and arginine digestibility were higher in S6D3 group than other groups. The ratio of CD4 and CD8 positive lymphocytes during the overall experimental period was independent of the ratio of SDPP and DPS. However, CD4+:CD8+ ratio was numerically lowered in pigs fed diet the S6D3 diet. Therefore, the present study suggests that an optimal inclusion ratio for maximizing growth performance and maintaining low immune status is 6% of SDPP and 3% of DPS in weaned pigs.
Progress in the development of heating systems towards long pulse operation for KSTAR
Kwak, J.G.,Bae, Y.D.,Chang, D.H.,Chang, D.S.,Hong, B.G.,Hwang, C.K.,In, S.R.,Jeong, S.H.,Jin, J.T.,Jung, K.S.,Kim, B.R.,Kim, J.,Kim, S.K.,Kim, T.S.,Lee, D.W.,Lee, K.W.,Oh, B.H.,Seo, C.S.,Seo, M.S.,Yoo International Atomic Energy Agency 2007 Nuclear fusion Vol.47 No.5
<P>Construction of the Korea superconducting tokamak advanced research (KSTAR) tokamak is in its final phase. For the long-pulse KSTAR discharges, the ion cyclotron range of frequencies (ICRF) and neutral beam injection (NBI) heating systems are expected to play important roles through a selective heating of ions and electrons, control of the plasma pressure and current profiles, a core fuelling and beam diagnostics for the KSTAR. In addition, the ICRF system is expected to be used for possible discharge cleaning and assisting in the tokamak startup. In this paper, the recent progress in the development of the ICRF and the NBI heating systems is described. The four-strap ICRF antenna has been successfully tested for a voltage up to 41 kV for a pulse length of 300 s (to 46 kV for 20 s) in a test chamber. A prototype KSTAR NBI system has been developed. At present, the system has successfully produced a 1 MW beam power for 200 s and a 3.5 MW output beam power for 4 s.</P>
국산박류의 사료가치에 관한 연구 2 . 부로일러에 대한 박류비교시험
백인기,한인규,김춘수 ( In K . Paik,In K . Han,Chun S . Kim ) 한국축산학회 1975 한국축산학회지 Vol.17 No.4
An experiment was conducted to study the comparative feeding value of locally produced vegetable protein cakes such as soybean oil meal (44%) (S.B.O.M.), rapeseed oil meal (R.O.M.), perilla oil meal (P.O.M.), sesame oil meal (S.O.M.) and corn gluten (50%). Three hundred broiler chickens were used for 8 weeks feeding trial and successive metabolic tiral. Results obtained are as follows; 1. Weight gains were significantly (P$lt;0.01) different among treatments showing the best gain in S.B.O.M. group and then P.O.M., R.O.M., corn gluten and S.O.M. group in that order. Feed intake showed same trend as weight gain did. For the feed efficiency, S.B.O.M. group was significantly (P$lt;0.01) superior to the other groups and S.O.M. group was significantly inferior to the other groups. Among the groups of receiving R.O.M., P.O.M. and corn gluten, differences were not significant each other in feed efficiency. 2. Amino acids compositions of R.O.M. and S.B.O.M. were better than that of other protein feeds considering their relatively high E.A.A. Index of 76.32 and 76.21 respectively. P.O.M., S.O.M. and corn gluten were low in lysine content while methionine contents were relatively high. 3. Nitrogen corrected M.E. values of S.B.O.M., R.O.M., P.O.M., S.O.M. and corn gluten were 2,367㎉, 843㎉, 2.234㎉, 1,305㎉ and 4,283㎉, respectively. Rates of nitrogen retention of P.O.M., S.B.O.M., R.O.M., corn gluten and S.O.M. were 59.38%, 51.18%, 54.11%, 44.80%, and 36.01%, respectively. 4. Dry matter and crude protein availabilities of finisher diet of R.O.M. group and S.B.O.M. group were higher than that of other groups but not significantly different. On the contrary however, crude fat availabilities of R.O.M., and S.B.O.M. group were significantly (P$lt;0.01) lower than that of other groups. 5. Thyroid gland weights and total serum cholesterol contents were significantly (P$lt;0.05) different among treatments. However, the relationship between thyroid gland weight and cholesterol content was observed only in R.O.M. group. Corn gluten was verb effective to increase skin pigmentation. 6. Production cost for 1㎏ of broiler meat of S.B.O.M. group was lower by about 20 won to 30won than that of R.O.M., corn gluten, or P.O.M. group, and by about 100won than that of sesame oil meal group which was least for the performance among the treatments.
Jeon, Jinwoo,Uthaman, Saji,Lee, Jiyoung,Hwang, Hyejin,Kim, Gibum,Yoo, Pil J.,Hammock, Bruce D.,Kim, Christine S.,Park, Yeon-Su,Park, In-Kyu Elsevier 2018 Sensors and actuators. B, Chemical Vol.266 No.-
<P><B>Abstract</B></P> <P>Over-secretion of cortisol from the adrenal cortex is closely related to acute and chronic stress; thus, rapid and sensitive detection of cortisol in serum is of critical importance for preventing the progression of stress-related diseases. The binding of a biological molecule to the surface of metallic nanoparticles changes the local refractive index and in turn induces a shift in the localized surface plasmon resonance (LSPR) wavelength. Utilizing this phenomenon, we designed a novel disposable LSPR-based cuvette-type sensor for detecting cortisol in serum. The developed cuvette-type nanosensor consists primarily of an assembly of plastic unit sensors coated with gold nanoparticles on a single layer wherein cortisol-conjugated bovine serum albumin (BSA) is immobilized. In this system, a redshift in LSPR wavelength is induced by the binding of cortisol antibody onto cortisol-conjugated BSA immobilized on a gold nanoparticle surface in the nanosensor. In a competitive assay, the nanosensor could rapidly detect cortisol in both a PBS solution and serum (within 20 min) at concentrations ranging from 1 to 10,000 ng/mL (2.759–3 × 10<SUP>3</SUP> nmol/L), which is comparable to conventional enzyme-linked immunosorbent assay (ELISA) which typically requires longer than 4 h and complex sample preparation. Thus, we demonstrated that the LSPR-based nanosensor system developed in this study can provide a useful toolkit for a rapid, highly sensitive and reliable detection of cortisol hormone in a commercially available manner.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Novel disposable LSPR-based cuvette-type sensor for detecting cortisol in serum. </LI> <LI> Rapid, convenient, and sensitive method for detecting cortisol hormone in serum. </LI> <LI> Detect cortisol in both PBS solution and serum within 20 min at concentrations ranging from 1 to 10,000 ng/mL. </LI> </UL> </P>
Jung, In-Hyuk,Choi, Jae-Hoon,Jin, Jing,Jeong, Se-Jin,Jeon, Sejin,Lim, Chaeji,Lee, Mi-Ran,Yoo, Ji-Young,Sonn, Seong-Keun,Kim, Young Ho,Choi, Beom Kyu,Kwon, Byoung S.,Seoh, Ju-Young,Lee, Cheol Whan,Kim, The Federation of American Societies for Experimen 2014 The FASEB Journal Vol.28 No.11
<P>CD137 (4-1BB), a member of the tumor necrosis factor receptor superfamily, has been reported to be expressed in atherosclerotic plaques, and to promote lesion formation. However, the role of CD137 in mediating atherosclerotic plaque stability and the possible underlying molecular and cellular mechanisms are poorly understood. Here, apolipoprotein E-deficient (<I>ApoE</I><SUP>−/−</SUP>) and CD137-deficient <I>ApoE</I><SUP>−/−</SUP> (<I>ApoE</I><SUP>−/−</SUP>CD137<SUP>−/−</SUP>) mice fed a chow diet for 66 wk were used. CD137 induces plaque instability, which is characterized by increased plaque necrosis, decreased collagen content, decreased vascular smooth muscle cell (VSMC) content, and increased macrophage infiltration. CD137 also increases the infiltration of effector T (T<SUB>eff</SUB>) cells into plaque lesion sites, resulting in increased interferon-γ (IFN-γ) expression. Interestingly, T<SUB>eff</SUB>-cell-derived IFN-γ inhibits collagen synthesis in atherosclerotic plaques. Furthermore, CD137 activation increases the apoptosis of VSMCs, possibly by decreasing the antiapoptotic regulator, Bcl-2, and subsequently up-regulating cleaved caspase-3. In macrophages, activation of CD137 signaling boosted the oxidized low density lipoprotein-induced expression of matrix metalloproteinase 9 <I>via</I> the p38 mitogen-activated protein kinase and extracellular signal-regulated kinase1/2 signaling pathways. In summary, activation of CD137 signaling decreases the stability of advanced atherosclerotic plaques <I>via</I> its combined effects on T<SUB>eff</SUB> cells, VSMCs, and macrophages.—Jung, I.-H., Choi, J.-H., Jin, J., Jeong, S.-J., Jeon, S., Lim, C., Lee, M.-R., Yoo, J.-Y., Sonn, S.-K., Kim, Y. H., Choi, B. K., Kwon, B. S., Seoh, J.-Y., Lee, C. W., Kim, D.-Y., Oh, G. T. CD137-inducing factors from T cells and macrophages accelerate the destabilization of atherosclerotic plaques in hyperlipidemic mice.</P>
Microbacterium xylanilyticum sp. nov., a xylan-degrading bacterium isolated from a biofilm
Kim, Kwang Kyu,Park, Hye Yoon,Park, Wooshin,Kim, In S.,Lee, Sung-Taik Microbiology Society 2005 International journal of systematic and evolutiona Vol.55 No.5
<P>A novel xylan-degrading bacterium, S3-E<SUP>T</SUP>, was isolated from the biofilm of a membrane bioreactor. The cells of this strain were Gram-positive, non-motile, non-spore-forming rods, produced primary branches and formed yellow colonies on nutrient agar. The strain had chemotaxonomic markers that were consistent with classification in the genus <I>Microbacterium</I>, i.e. MK-12, MK-11 and MK-13 as the major menaquinones, predominant iso- and anteiso-branched cellular fatty acids, glucose and galactose as the cell-wall sugars, peptidoglycan-type B2<I>β</I> with glycolyl residues and a DNA G+C content of 69·7 mol%. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed that strain S3-E<SUP>T</SUP> is most similar to <I>Microbacterium hominis</I> IFO 15708<SUP>T</SUP> and <I>Microbacterium foliorum</I> DSM 12966<SUP>T</SUP> (97·6 and 97·4 % sequence similarity, respectively), and that it forms a separate lineage with <I>M. hominis</I> in the genus <I>Microbacterium</I>. DNA-DNA hybridization results and phenotypic properties showed that strain S3-E<SUP>T</SUP> could be distinguished from all known <I>Microbacterium</I> species and represented a novel species, for which the name <I>Microbacterium xylanilyticum</I> sp. nov. is proposed; the type strain is S3-E<SUP>T</SUP> (=DSM 16914<SUP>T</SUP>=KCTC 19079<SUP>T</SUP>).</P>
Kim, Min Chul,Jeong, Myung Ho,Sim, Doo Sun,Hong, Young Joon,Kim, Ju Han,Ahn, Youngkeun,Ahn, Tae Hoon,Seung, Ki Bae,Choi, Dong-Joo,Kim, Hyo-Soo,Gwon, Hyeon Cheol,Seong, In Whan,Hwang, Kyung Kuk,Chae, S Elsevier 2018 The American journal of cardiology Vol.121 No.11
<P>The optimal timing of percutaneous coronary intervention (PCI) in patients with non–ST-segment elevation myocardial infarction (NSTEMI), complicated by acute decompensated heart failure (ADHF), is unclear. A total of 1,027 patients with NSTEMI complicated by ADHF who underwent successful PCI were analyzed using a Korean multicenter registry. All patients were divided into 4 groups by the timing of PCI: group 1 (PCI < 2 hour after admission, n = 149), group 2 (2 to 24 hours, n = 577), group 3 (24 to 72 hours, n = 189), and group 4 (≥72 hours, n = 112). We analyzed the incidences of 12-month mortality, nonfatal myocardial infarction (MI), target-vessel revascularization, and rehospitalization because of HF. The prevalence of ADHF in patients with NSTEMI was 15.2% at initial presentation, and in-hospital mortality was higher in group 1 than in the other groups. There were no significant differences in mortality, nonfatal MI, target-vessel revascularization, or rehospitalization for HF during the 12-month follow-up between groups, regardless of initial PCI timing, except for a higher 12-month mortality in patients who received PCI within 24 hours (vs ≥24 hours) (hazard ratio 1.52, 95% confidence interval 1.09 to 2.29, p = 0.046). Early PCI did not reduce adverse clinical outcomes in patients with NSTEMI complicated by ADHF. Delayed PCI after stabilization may be reasonable in such high-risk patients.</P>