경상계 지역에 대한 AVHRR 자료의 지질적 상관성 연구 (Ⅱ) : 복사율 및 스펙트럼 지수 Emissitivity and TISI

박종남,박인석 동아대학교 건설기술연구소 1997 硏究報告 Vol.21 No.2

A study was made on geological correlations of the NOAA/AVHRR data and their geothermal applicability for the Kyungsang area. Following the paper on the land surface temperature and the thermal inertia for the Kyungsang area by Park et at(1993), the emissitivity and TISI(Temperature Independent Spectral Index) have been studied for the same data. Even though it is difficult to find out close relationships to the regional geology, it was confirmed that the emissivity and TISI images are of great use even with noise effects due to weather condition or topography which might not be possible to remove easily.

Foreword

박인석(Innseok Park) 한국디자인학회 2016 한국디자인학회 국제초대전도록 Vol.2016 No.12

Human Chorionic Gonadotropin(HCG)에 의한 넙치, Paralichthys olivaceus의 배정효과

박인석,김형배,최희정,이영돈,손진기 제주대학교 해양연구소 1992 해양자원연구소연구보고 Vol.16 No.-

For the evaluation of hormonal control of sperm in cultured fish a method to quantify the spermiation response of mature flatfish to hormonal therapy is described. Spermatocrit was determined after 30 min. in centrifuge condition of 12,000rpm. Sperm density was estimated by a standard hemacytometer method. However, sperm density can be predicted from spermatocrit since their relationship is linear as described by the re-gression equation. Y=1.14X-0.04 (r=0.91. P<0.0001. n=50), where Y is spermatocrit and X is sperm density. Sperm density levels ranged from 21.5 to 98.4×10^(4) spermatozoa per ㎕ milt corresponding to spermatocrit values of 12 to 95%. Milt production by mature flatfish was highest (7.4ml per kg body weight) at 24h after injection of 1,000IU of human chorionic gonadotropin(HCG) per kg body weight and coincided with low spermatocrit (63%) and sperm density (67.4×10^(4) spermatozoa per ㎕ milt) levels. No significant differences was appeared in milt production, spermatocrit and sperm density between control and HCG-treated fish at 48h after HCG injection. These results demonstrate that spermiation in mature flatfish can be reliably evaluated by a spermatocrit method and that HCG is effective in stimulating of spermiation in this species.

암수동체성 어류 Rivulus marmoratus의 치어에 미치는 몇가지 화학적 발암원의 급성 독성에 관하여

박인석,박은호 漢陽大學校環境科學硏究所 1987 環境科學論文集 Vol.8 No.-

어독성 검출계로서 암수동체성 어류 Rivulus marmoratus의 가치성을 살펴보고자 본종의 치어에 미치는 N-methyl-N'-nitro-N-nitrosoguanidine(MNNG), N-nitroso-N-methylurea(MNU)와 aflatoxin B₁(AFB₁)의 급성독성을 조사한 결과는 아래와 같다. 1. MNNG의 24hr-??값은 37.7ppm이었고 95% 신뢰한계의 상한치는 38.5ppm, 하한치는 37.0ppm이었다. MNU의 24hr-?? 값은 96.0ppm이었고 상한치는 97.9ppm, 하한치는 94.2ppm이었으며 AFB₁의 24hr-?? 값은 0.94ppm이었고 95% 신뢰한계의 상한치는 0.98ppm, 하한치는 0.90ppm이었다. 2. 모든 처리화합물은 본종에 대해 좁은 농도범위 내에서 급성 독성 효과를 나타내었으며 아울러 뚜렷한 농도반응을 보여주었다. 이상의 결과를 본종이 지닌 어독성 연구 실험동물로서의 가치성과 연관시켜 논의하였다. The acute toxicities of N-methyl-N'-nitro-N-nitrosoguanidine(MNNG), N-nitroso-N-methylurea(MNU) and aflatoxin B₁(AFB₁) to the hermaphroditic fish Rivulus marmoratus larvae were examined to evaluate the possibility of the use of this fish as the assay model of the aquatic toxicology. The results obtained were as follows; 1. The 24hr-?? to MNNG was 37.7 ppm with 95% confidence range value of 38.5~37.0ppm. The ?? for 24hours to MNU and AFB₁were found to be 96.0ppm and 0.94ppm, respectively. Their 95% confidence ranges were 97.9~94.2ppm in MNU and 0.98~0.90ppm in AFB₁. 2. The R. marmoratus larvae responded to narrow range of concentration of all tested chemicals, and toxic effects of the chemicals were clearly dose dependent. The above results were discussed in relation to the usefulness of R. marmoratus as a suitable model for aquatic toxicology.

유도된 연어과 잡종의 유합쌍유생

박인석,노재구,남윤권,김동수 한국어병학회 2001 한국어병학회지 Vol.14 No.3

무지개송어(Oncorhynchus mykiss)×은연어(O. kisutch)간 유도 잡종에서 유합쌍유생이 발견되었다. 잡종은 부화 개체 중 22.5%의 기형율을 보였고, 이 중 17.3%의 개체가 유합쌍유생이었다. 이러한 유합쌍유생은 3종류의 유합쌍유생: (1) 동일한 난황에 정상의 2 유생 머리가 유합한형, (2) 두 갱의 유어 머리가 동일한 난황과 몸통에 유합한 형, (3) 동일한 난황에 2 유생이 기형적으로 유합한 형을 보였다. We present conjoined twins occurring in the hybridization between rainbow trout(Oncorhynchus mykiss) and coho salmon(O. kisutch), Hybrid cross displayed significantly higher abnormality (ranging 20 to 26%) than pure crossed (less than 1.5%). The incidence of conjoined twin among the abnormal lavea in the hybrid cross was as high as 17.3%. Three different types of conjoined twins were observed : (1) two larvae connected to the same yolk sac in an otherwise and normal fashion, (2)two heads of the larvae connected to the same body with one yolk sax, and (3)two larvae connected to the same yolk sac in an otherwise but abnormal fashion.

버들치, Rhynchocypris oxycephalus (Sauvage and Dabry) 기아시 일부형질에서의 효과 : 개관 A Review

박인석 한국환경생물학회 2004 환경생물 : 환경생물학회지 Vol.22 No.3

기아시와 포식시 버들치, Rhynchocypris oxycephalus (Sauvage and Dabry)에 미치는 영향을 파악하기 위하여 조직학적 및 생화학적 변화, 체절단면 계측형질 변화 그리고 계측형질 변화에 대하여 조사하였다. 기아에 따른 버들치의 생화학적, 조직학적 변화를 파악하기 위해, 간의 간중량지수, 단백질함량, RNA량 및 DNA량 조사와 더불어 간세포 및 중장 상피세포의 조직학적 조사를 실시한 결과, 기아군은 포식군에 비해 간의 단백질함량, RNA량 및 DNA량에서 높은 값을 보였지만, 먹이 공급 중단은 버들치의 간중량지수, 간세포 핵 크기 및 중장 상피세포 핵 높이에 있어서 감소를 나타내었다. 본 실험에서 조사된 RNA-DNA비는 버들치의 영양상태 파악 지수로 유용하였으며 또한, 버들치 표본 시기에서의 높은 성장 단계인지 혹은 낮은 성장 단계인지의 성장 활성 파악을 가능케 하였다. 본 연구 결과의 자료를 구체적으로 해석 하였으며, 아울러 다수의 생물학적 중요 연관 사항들을 고찰하였다. 버들치 기아시의 영양상태 평가를 위하여 비만도, 내장괴지수, Dressing비를 비롯한 체횡단 절단면에서의 형태 적변화를 조사하였다. 9주간의 기아시 비만도, 내장괴지수 및 대부분의 체횡단 절단면 계측형질들에서의 감소를 보였다(P<0.05). 본 연구 결과, 실험에 적용된 영양성 Parameter는 본 종의 영양상태 파악에 유용한 지표임을 시사한다. 연구 결과를 자세히 해석하여, 버들치에서의 주요 체 절단 형질의 산업성을 논의하였다. 버들치에서의 75일간 기아가 Classical dimension과 Truss dimension에 미치는 영향을 파악하였다. Truss dimension인 경우 포식시와 기아시 복강을 비롯한 머리부위와 몸통부위가 유의하게 증가하였다(P<0.05). Truss dimension의 꼬리부위 특히, 몸통의 뒷부분이 포식과 기아시 일반적으로 감소하였다. Classical dimension인 경우 포식시 꼬리부위가 감소한 반면, 기아시 몸통부위와 꼬리부위에서의 체고 관련 형질에 연관시 머리부위에서의 형질들이 유의하게 감소하였다. 본 연구 결과 Truss dimension은 Classical dimension을 보완하여 어체의 수직방향 연관 형질 파악도 가능케 하였다. Truss dimension과 Classical dimension인 경우 포식시와 기아시 머리 아랫부위는 거의 영향이 없어, 본 형질은 버들치속 어류의 분류학적측면에서 유용하게 사용될 수 있을 것이다. 버들치에서 체고와 연관된 몸통부위 형질들은 상이한 서식지에 기인된, 다양한 먹이공급 차이의 파악에 유용하리라 사료된다. Following the previous experiments, a starvation experiment was conducted to determine the influence of feeding and starvation on the histological and biochemical changes, the morphormetric changes in the sectioned body and the morphometric changes in Rhynchocypris oxycephalus (Sauvage and Dabry). The influence of starvation on nutritional conditions of the histological changes of hepatocyte and intestinal epithelium as hepatosmatic index (HSI), protein, RNA and DNA concentrations of liver in R. oxycephalus was tested. Although the starved group showed higher concentrations of protein, DNA and RNA than the fed group, food deprivation resulted in a decrease in the HSI, hepatocyte nucleus size and nuclear height of the intestinal epithelium. The RNA-DNA ratio appears to be a useful index of nutritional status in R. oxycephalus and may be useful for determining if R. oxycephalus is in a period of rapid or slow growth at the time of sampling. Additionally, the data have been interpreted in detail and some biologically important relationships discussed. The effects of starvation on the morphometrical changes in sectioned body traits, condition factor, viscera index and dressing percentage were determined for evaluating nutritional conditions of R. oxycephalus. Starvation for nine weeks resulted in a decrease in most sectioned traits as well as in condition factor and viscera index (P<0.05). These findings suggest that nutritional parameters used in this study appear to be a useful index for nutritional status in this species. The data has been interpreted in detail and some important body sectioned values of interest to commercial growers discussed. A 75-day study was conducted to determine the effect of starvation on classical and truss parameters in R. oxycephalus. Truss dimensions of almost the entire head and trunk region as well as the abdomen were increased significantly through feeding or starvation (P<0.05). Truss dimensions of the caudal region generally decreased through feeding or starvation, particularly those dimensions at the hind part of the trunk. There were some significant decreases in classical dimensions of the head region during feeding, in relation to body depth characteristics in the trunk and caudal region during starvation, whereas there was only one decreasing classical dimension in the caudal region during feeding. The results of this study indicate that application of the truss network as a character set enforces classical coverage across the body form, discrimination among experimental groups thus being enhanced. Considering that the dimension of the lower part of the head and some truss and classical dimensions were least affected by feeding and starvation, these dimensions may then be useful as a taxonomical indicator to discriminate the species of Rhynchocypris sp. The value of trunk region dimensions with a large component of body depth in R. oxycephalus is most likely to be compromised by variability related to differences in feeding regimes of fish in different habitats.

Thyroxine과 Iodide에 의한 버들치, Rhynchocypris oxycephalus (Sauvage and Dabry) 성장 효과

박인석,임재현,조성환 한국어류학회 2004 韓國魚類學會誌 Vol.16 No.1

Thyroxine과 iodide의 먹이공급 시 버들치, Rhyn-chocypris oxycephalus (Savage and Dabry) 성장에 미치는 효과를 조사하였다. 부화 후 120일의 자어(평균 전장 4.48㎝, 평균체중 0.84g)를 실험에 사용하였으며, Na-I-thyroxine(T4)을 최종농도 1 ppm과 5 ppm으로, Na-iodide(I)를 최종농도 1 ppm, 5 ppm 및 25 ppm로 잉어사료에 분사시켰다. 대조군은 이를 처리하지 않은 잉어사료만을 공급하였다. 먹이는 실험기간인 12주 동안 만복에 가깝도록 충분히 공급하였다. 실험기간 중 대조군은 97.5%의 생존율을 보였고, 실험군들은 대조군의 생존율과 유사하였으며 기형은 관찰되지 않았다. 5 ppm T₄로 먹이 공급시 어체중 증가(%)와 일일성장륙이 가장 높았으며 1 ppm Ⅰ군, 대조 군, 25 ppm Ⅰ군, 5 ppm Ⅰ군, 1 ppm T₄군의 순으로 어체중 증가와 일일성장률을 보였다. 일일사료섭취율에서 5 ppm T₄군과 1 ppm Ⅰ군은 실험군과 대조군에 비해 유의하게 컸으며(P〈0.05), 25 ppm Ⅰ군은 가장 낮은 일일사료섭취율을 보였다. 사료전환효율은 25 ppmⅠ군이 가장 높았으며 5 ppmⅠ군, 1 ppm T₄군, 대조군, 1 ppmⅠ군, 5 ppm T₄군의 순으로 사료전환효율을 보였다. 5 ppm T₄군에서의 높은 어체중 증가와 높은 일일성장률은 높은 일일 사료섭취율에 기인되었으며 낮은 사료전환효율을 보였다. 1 ppmⅠ 먹이공급군의 비만도는 대조군이나 다른 실험군에 비해 유의하게 높았다(P〈0.05). 본 실험 결과 5 ppm T₄와 1 ppm Ⅰ 첨가는 본 종의 성장 촉진에 유의하게 효과적이었다. Twelve-week feeding trial was performed to investigate effectiveness of dietary thyroxine and iodide on growth of Rhynchocypris oxycephalus (Sauvage and Dabry). One hundred and twenty-day old larvae (4.48 cm in mean total length and 0.84 g in mean wet body weight) were used as the experimental fish and acclimated into the experimental conditions. Two levels of concentrations (1 and 5 ppm) of sodium-L-thyroxine (T₄) and three levels of concentrations (1, 5 and 25 ppm) sodium iodide (Ⅰ) were sprayed on commercial feed for carp. Commercial feed for carp without spraying T₄ or I was used as control. Fish were fed to visual satiety for 12 weeks, Water quality was regularly monitored throughout the feeding trial. The control group showed 97.5% survival. Fish in the experimental groups at the end of the feeding trial achieved similar survival to control group and their appearance was to be normal without any deformity in all treatments. Weight gain (%) and specific growth rate of fish fed the diet with T₄, at 5 ppm were highest, and Ⅰ at 1 ppm, control, Ⅰ at 25 ppm, Ⅰ at 5 ppm and T₄ at 1 ppm, in order. Daily feed intake of fish fed on the diet with T₄, at 5 ppm and Ⅰ at 1 ppm were significantly (P< 0.05) higher than that of fish fed on the other diets. Daily feed intake of fish fed on the diet with Ⅰ at 25 ppm was highest. Feed efficiency ratio for fish fed on the diet with Ⅰ at 25 ppm was highest followed by Ⅰ at 5 ppm, T₄ at 1 ppm, control, Ⅰ at 1 ppm and T₄ at 5 ppm in order. This indicated that high weight gain and specific growth rate for fish fed the diet with T₄ at 5 ppm resulted from high daily feed intake, and eventually led to low feed efficiency ratio. Condition factor of fish fed the diet with Ⅰ at 1 ppm was significantly (P< 0.05) higher than that of fish fed the other diets. Results of this study indicate that 5 ppm T₄, and 1 ppm Ⅰ treatment were effective for growth promotion in R. oxycephalus.

기아시 버들치 Rhynchocypris oxycephalus(Sauvage and Dabry)의 일부 영양조건에서의 효과 : 2. 체 절단면 계측형질의 변화 2. Characteristics of the Morphometric Changes in the Sectioned Body

박인석,임재현,정창화,노재구,김윤해,이영호 한국어류학회 2002 韓國魚類學會誌 Vol.14 No.1

버들치 Rhynchocypris oxycephalus(Sauvage and Dabry) 기아시의 영양 상태 평가를 위하여 비만도, 내장괴지수, dreessing 비를 비롯한 체 횡단 절단면에서의 형태적 변화를 조사였다. 9주간의 기아시 비만도, 내장괴지수 및 대부분의 체 횡단 절단면 계측 형질들에서의 감소를 보였다(P<0.05). 본 연구 결과, 실험에 적용된 영양성 parameter는 본 종의 영양 상태 파악에 유용한 지표임을 시사한다. 연구 결과를 자세히 해석하여, 버들치에서의 주요 체 절단 형질의 산업성을 논의하였다. The effects of starvation on the morphometrical changes in sectioned body traits, condition factor, viscera index and dressing percentage were determined for evaluating nutritional conditions of Rhynchocypris oxycephalus(Sauvage and Dabry). Starvation for nine weeks resulted in a decrease in most sectioned traits as well as in condition factor and viscera index(P<0.05). These findings suggest that nutritional parameters used in this study appear to be a useful index for nutritional status in this species. The data has been interpreted in detail and some important body sectioned values of interest to commercial growers discussed.

염색체 조작에 의한 어류의 생물공학

박인석 제주대학교 해양연구소 1993 해양자원연구소연구보고 Vol.17 No.-

The manipulation of chromosome becomes feasible during the nuclear cycles of cell division and basically comprkes the addition or subtraction of a complete haploid or diploid set. Two basic fields of practical importance involve the processes of parthenogenesis and induced polyploidy, respectively. Polyploidy includmg triploid and tetraploid is produced using physical or chemical treatments for disrupting metaphase. Gynogenesis and androgenesis is induced with sperm or eggs inactivated by radiation. Triploids are of interest because they are expected to be sterile, to grow faster than diploids as they reach the age of sexual maturity, and to live longer than diplpids because of the biological costs associated with reproduction More recently. triploids have been produced in a wide variety of fish and promises soon to be a routine tool in fish breeding. Tetraploids are not so for confirmed in fish but remains a desirable goal because of its potential for further production of triploids or for line breeding of otherwise difficult hybrids. Gynogenetic diploids have been used for cjtogenetic studies of meiotic phenomena and gone mapping. Mitotic gynogenetic diploids and androgenetic diploids are completely homozygous and similar to inbred or isogenic lines. Chromosome engineering has immediate applicability in fish farming and great promise for the further domestication of fish.

산천어, Oncorhynchus masou의 성분화

박인석,이영돈,정의영 제주대학교 해양연구소 1997 해양자원연구소연구보고 Vol.21 No.-

Determing the initial treatment time and duration of treatment for sex reversal in fish is important. In this study the formation of gonad and their manner of sex differentiation in cherry salmon. Oncorhynchus masou was histologically examined. Primordial germ cell in mean total length 2.24 cm fry (24 days after hatching. accumulated temperature 194℃ ) appeared between mesonephric dut and gut. Genital ridge development was predominant in larva (36 days after hatching, accumulated temperature 294℃ ) with primordial germ cells(mean diameter of 14.2㎛) in active mitosis. Sex differentiation initiated at 40 days after hatching(mean total length 2.87cm. accumulated temperature 326℃ ) and ovarian cavity and efferent duct appeared in larva(mean total length of 3.25 cm) 44 days after hatching. Larva with a mean total length of 5.90 cm in 104 days after hatching (accumulated temperature 890℃) showed differentiated gonad and female and male sex ratio in this stage is 1:1.04(P<0.01). Above result revealed that gonad differentiation pattern of cherry salmon is differentiated gonochorism.