Structural analysis and bioactivities of a polysaccharide isolated from Capsosiphonfulvescence (Masaengi)

Doo Jin Choi,Ji Won Choi,Jisun Lee,Chang Won Lee,Seul Lee,Andriy Synytsya,Yong Il Park 한국당과학회 2013 한국당과학회 학술대회 Vol.2013 No.1

A water-soluble polysaccharide (SPS-CF) was isolated and purified from Korean green alga Maesaengi Capsosiphon fulvescens mainly by dilute acid extraction, ethanol precipitation, and DEAE-cellulose ion exchange chromatography. The results of present study performed for monosaccharide composition of this polysaccharide using HPLC of PMP labelled sugars, HPAEC-PAD, GC-FID and TLC, clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (44–52 mol %) and rhamnose (34–45 mol %), while mannose and galactose are present at much lesser extent or in negligible amount. These extensive monosaccharide analyses and correlation NMR measurements confirmed that it is a sulphated glucuronorhamnoxylan (ulvan) type polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains. The SPS-CF exhibited significant invitro anticoagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged. Also, Enzyme Linked Immunosorbent Assay showed that the SPS-CF significantly stimulates the release of the pro-inflammatory cytokines, TNF-α and IL-6, in a dose-dependent manner. RT-PCR analysis demonstrated that the SPS-CF also induced a more than two-fold increase in the expression of iNOS and COX-2, responsible for the induction of NO and PGE2, respectively, at 5 μg/ml in RAW264.7 murine macrophages. The results of this study demonstrated that the SPS-CF isolated from Korean C. fulvescens Maesaengi is an ulvan type polysaccharide and can be considered as potential anticoagulant and immunostimulating agent.

A soft biomolecule actuator based on a highly functionalized bacterial cellulose nano-fiber network with carboxylic acid groups

Wang, Fan,Jeon, Jin-Han,Park, Sukho,Kee, Chang-Doo,Kim, Seong-Jun,Oh, Il-Kwon The Royal Society of Chemistry 2016 SOFT MATTER Vol.12 No.1

<P>Upcoming human-related applications such as soft wearable electronics, flexible haptic systems, and active bio-medical devices will require bio-friendly actuating materials. Here, we report a soft biomolecule actuator based on carboxylated bacterial cellulose (CBC), ionic liquid (IL), and poly (3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) electrodes. Soft and biocompatible polymer-IL composites were prepared via doping of CBC with ILs. The highly conductive PEDOT: PSS layers were deposited on both sides of the CBC-IL membranes by a dip-coating technique to yield a sandwiched actuator system. Ionic conductivity and ionic exchange capacity of the CBC membrane can be increased up to 22.8 times and 1.5 times compared with pristine bacterial cellulose (BC), respectively, resulting in 8 times large bending deformation than the pure BC actuators with metallic electrodes in an open air environment. The developed CBC-IL actuators show significant progress in the development of biocompatible and soft actuating materials with quick response, low operating voltage and comparatively large bending deformation.</P>

枸杞子나무의 果實成熟에 관한 硏究 : 1. 成熟過程에 있어서 果實의 形態的 變化 1. Changes of morphological and histological characteristics of fruit during ripening

陳日斗,李相來 順天大學校 1984 論文集 Vol.3 No.1

優良 구기자의 증산을 위한 기초적 硏究로 구기자과실의 成熟에 따른 形態的 變化 過程과 우량 구기자의 특성을 調査하여 다음과 같은 結果를 얻었다. 1. 枸杞子의 果實은 子房壁, 격벽, 胎座 및 種子의 4部分으로 구성되어 있었다. 2. 枸杞子의 果實은 開化後 約 50日 이면 완전히 成熟하여 紅熟果로 되었다 3. 果實의 길이는 開花後 15日 까지 거의 伸長이 完了되는데 開花期로부터 10일까지의 기간에 급격히 伸長하였다. (Fig.1) 4. 果實의 幅은 開花로부터 開花後 10日 사이에 급격히 肥大하며 開花後 30~50일 사이에도 크게 肥大하였다. (Fig1) 5. 子房壁은 1列의 外表皮와 8-12列의 皮層, 2列의 內表皮로 構成되어 있었고 皮層에는 維管束이 散在되어 있었으며, 격벽의 양외측에 子房壁의 內表皮와 비슷한 2列의 細胞層이 있었다. 격벽의 中央에 胎座가 있었으며 胎座에는 4개의 維管束이 果實의 길이 方向으로 縱走하고 있었다. (Fig.2-①.②.③) 6. 開花後 3日째는 維管束에 導管만 分化되어 있었으나 7日째에는 篩管도 分化되어 있었고 外表皮의 細胞壁이 다소 肥厚되어 있었다. (Fig.2-④.⑤.⑥) 7. 開花後 10日째에는 子房壁의 皮層 組織에서 葉綠체를 관찰할수 있었으나 開花後 40日째에는 葉綠체가 소멸되어 있었다. (Fig.2-⑦.⑧.⑨.⑩) 8. 격벽의 細胞는 分裂없이 肥大 生長하였으나 珍島地方 栽培種의 경우 子房壁의 細胞는 開花期에 比해 成熟期에 1~2層 增加하였다. (Table 1) 9. 子房壁과 胎座는 開花 30日 後 급격히 肥大하였다. (Table 1) 10. 珍島地方栽培種은 靑陽地方栽培種에 比해 果實의 길이가 길고 幅이 좁았다. (Table 2). 11. 早期成熟果에서 珍島地方 栽培種은 靑陽地方 栽培種에 비해 果實 무게가 가벼웠고 乾物重 比率도 낮았으며 種子數도 적었으나 晩期 成熟果에서는 乾物重 比率이 낮고 種子數가 적은 반면 果實의 무게는 무거웠다. (Table 3) 12. 子房壁과 격벽의 두께 및 격벽의 細胞層은 두 品種間에 큰 차이가 없었으나 子房壁의 細胞層은 珍島地方 栽培種이 靑陽地方 栽培種에 비해 많았다. (Table4) In order to study the characteristics of fruit of good variety, morphological and histological changes of fruit were investigated during ripening from flowering, using two varieties, Jindo local variety generally known as good variety and Chungyang local variety. The results obtained were summarized as follows; 1. The fruit of tea tree was found to be composed of pericarp, partition, placenta and seeds (Fig.2-①.②) 2. The ripening of fruit was required about 50 days after flowering. 3. The fruits had been elongated fully in length during 15 days after flowering, and rapid elongation was occurred during 10 days after flowering (Fig.1) 4. The width of fruit was enlarged firstly during 10 days after flowering, and secondly during period from 30 days to 50 days after flowering. (Fig.1) 5. The pericarp of fruit could be divided into epidermis, cortex and endodermis. And in both sides of partition, two layers of cells similar to those of endodermis were observed. In the cortex of pericarp and partition, vascular bundles were found to be located with the same interval, and there were four vascular bundles in the placenta (Fig.2-①.②.③) 6. There were phloems to be differentiated in vascular bundlest at 7 days after flowering while xylems were found at 3 days after flowering, and the thickening of epidermal cell wall in pericarp was observed at 7 days after flowering (Fig.2-④.⑤.⑥) 7. In the cortex of pericarp, chloroplasts were observed at 10 days after flowering but were not observed at 40 days (Fig.2-⑦.⑧.⑨.⑩) 8. The cells forming partition of two varieties and the pericarp of Chungyang local variety were enlarged without cell division during ripening while one or two layers of cell forming pericarp of fruit in Jindo local variety were increased during ripening (Table 1). 9. The cells forming pericarp and placenta were enlarged rapidly from 30 days after flowering (Table 1). 10. The length of fruit in Jindo local variety was longer than that in Chungyang local variety, where as the width of fruit was narrower in Jindo local variety (Table 2). 11. In the early ripened fruit, Jindo local variety was lighter in flesh and air-dried weight of fruit, lower in air-dried weight percentage to flesh weight and fewer in number of seeds per a fruit as compared with Chungyang local variety. In the late ripened fruit, flesh and air-dried weight of 100 fruits in Jindo local variety were larger than those in Chungyang local variety (Table 3). 12. The diameter of pericarp and partition, the size of cells forming partition were similar in both local varieties. However the size of cells forming pericarp in Jindo variety was smaller than that in Chungyang local variety (Feg.2 and Table 4).

MO이론에 의한 Diels-Alder 반응의 배향성과 반응성의 결정(Ⅰ)

丁學鎭,金一斗,朴聖珪 조선대학교 기초과학연구소 1985 自然科學硏究 Vol.8 No.1

This paper aims to predict the reactivity and the regioselectivity of Diels-Alder reactions of unsymmetical dienes and dienophiles. Frontier orbital theory has been applied to thermal Diels-Alder reaction by means of CNDO/2 and EHT-spd methods. The above reactions are normal electoron demand reaction and the major regioisomer is the para-substituents that is identical with experiments. Four-frontier orbitals and two-frontier orbitals' methods are identical with experimental major regioisomers. Sulfur is a much more polarizing substituent for the dienes than chlorine and cyanoradical. When sulfur is in diene, it shall dominate the regioselectivity. The coefficient polarization of substituted dienes and dienophiles is the same direction. We can predict the major regioisormer as para-substituents.

효율적인 네트워크 자원관리를 위한 원격제어 시스템의 설계 및 구현

최성두;김진일 東義大學校産業技術開發硏究所 2010 産業技術硏究誌 Vol.24 No.-

These days, as the business environment has changed, resources are expanding the IT field. In this matter, there are many problems in security and management of computers, so thorough management is required. In this paper, through design and implementation of a group policy for active directory could reduce managing manpower and TCO(Total Cost of Ownership) for network management against many existing network management problems in the areas of vaccine updates, OS patches and software upgrades etc. In addition, this system contribute installation for network management with no initial cost and maintenance cost reduction as well.

포름알데하이드에 폭로된 해부학 실습 학생들의 임파구 자매염색분체교환

백두진,주수자,김기영,이수진,심상효,손정일,심성한,송재철,조율희 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.2

Sister-chromatid exchanges measured in the peripheral lymphocytes of 15 non-smoking medical students after exposure to formaldehyde during 24-week anatomy class showed a small but significant (p=0.0468) increase when compared with samples obtained from the same individuals immediately before exposure. Mean frequencies of sister-chromatid exchange of cultured peripheral lymphocytes were 5.40±0.24 from the samples before exposure and 5.87±0.22 from the same samples after exposure. Breathing-zone air samples collected by formaldehyde monitoring kit with digital colorimeter(SKC) showed a mean concentration of 0.72±0.02 ppm formaldehyde.

주파수 대역별 PN부호화 연산을 통한 이미지 워터마킹

하진일,박수형,주동현,염동훈,김두영 동아대학교 정보기술연구소 2002 情報技術硏究所論文誌 Vol.10 No.1

Recently develpoing of the internet technology and spreading of the variable digital media are caused the problems. They are to protect the copy and to preserve the copyright. It is that the resolution is digital watermark. This paper is research about digital watermark that is to strengthend by PN encoding after Wavelet transform.

順川地方에 있어서 麥後作栽培에 의한 外來땅콩 品種의 出芽 開花및 收量構成要素에 대하여

朴瑞基,陳日斗,李東熏 順天大學校 1985 論文集 Vol.4 No.1

順天地方에 있어서 땅콩麥後作載倍에 適合한 品種 및 유망한 遺傳因子들을 선발하기 위하여 外國으로부터 導人된 Virginia type 94品種, Spanish type 99品種, Valencia type 18品種 및 其他 4品種等 計215品種을 供試하여 出芽, 開花 및 收量構成要素等을 調査하였던 바 다음과 같은 結果를 얻었다. 1. 播種後 出芽까지 所要日數는 5日에서부터 11日까지의 分布를 보였고 Virginia type品種들이 늦게 出芽하고 Valencia type品種과 Spanish type品種들이 빨리 出芽하는 傾向으로 나타났다. 2. 播種後 開花까지 所要日數는 33일부터 60日까지의 分布를 보였으며 Virginia type 品種이 늦게 開花하고, Spanish type, Valencia type 品種들이 빨리 開花하는 경향으로 나타났다. 3. 1株當收量에 있어서는 子實100粒重은 적으나 株當子實數가 많고 總子實數에 대한 完熟子實數比率이 높은 Valencia type品種이 많았으며, 같은 小粒種이면서 子實數가 적었던Spanish type品種과 子實 100粒重은 무거우나 子實數가 적고 總子實數에 대한 完熟子實數比率이 낮았던 Virginia type品種은 1株當收量이 적었다. 4. 出芽所要日數와 開花所要日數 및 100粒重 및 開花所要日數 와 100粒重과의 사이에 0.1%水準의 높은 正의 相關關係가 認定되었고 完熟種子數比率과 出芽所要日數, 開花所要日數 및 100粒重과의 사이에 0.1%水準의 높은 負의 相關關係, 100粒重과 株當種子數사이에 1%水準의 높은 負의 相關關係가 認定되었다. 5. 以上의 結果 收量面에서 생각한다면 小粒重인 Valencia type品種은 順天地方에서 麥後作으로 直接 移用할 수 있는 品種이 많이 포함되어 있으며 다른 type의 品種들이라 할지라도 品種間 差異가 다양하므로 그중에는 栽培에 유망한 品種들이 포함되어 있음을 알 수 있었다. 또한 各 品種들이 지닌 特異한 形質을 育種의 素材로 利用한다면 우리나라 南部地方에서의 多收性品種 또는 晩播適應性品種이 育成될 가능성이 있다는 結論을 얻었다. In order to find the genetic resource favorable and the varieties suitable for second crop of barley of Suncheon area, emergence, flowering and yield components of peanuts were investigated with total 215 varieties, 94 Virginia type, 99 Spanish type, 18 Valencia type and 4 others etc. introduced from abroad. The results obtained were summarized as follows; 1. Number o days required to emergence after seedings was in range of 5-10 days, and Valencia type and Spanish type emerged more rapidly than virginia type. 2. Number of days required to flowering after seedings was in range of 33-60 days, and Spanish type and Valencia type flowered more rapidly than Virginia type. 3. In weight of matured seeds per plant, Valencia type, which had light 100 seeds weight, abundant seeds per plant and high percentage of matured seeds to total seeds, had-more yield per plant than Spanish type, which had small seeds per plant, and Virginia type, which had heavy 100 seeds weight, small seeds per plant and low percentage of matured seeds to total seeds. 4. There were significant positive(+) correlations at 0.1% level between number of days to emergence and number of days to flowering, between number of days to emergence and 100 seeds weight and between 100 seeds weight and number of days to flowering, respectively. Relationship between percent seeds matured and mumber of days emergence and between number of days to flowering and 100 seeds weight was significantly negative(-) at 0.1% level, and between 100 seeds weight and number of seeds per plant at 1% level. 5. From the results above menentioned, it is suggested that, in view of yield, most varieties of Valencia type and a few varieties of other type can be cultivated as second drop of barley. In addition, it is determined that specific characteristics contained in some varieties are favorable genetic resoures for breeding of high yiedlding, early maturing or late seeding varieties adapted second cropping of barley, in southern region of Korea.

라벨링과 레벨세트 이론을 이용한 양방향 곡선 전개 형태 추출

박수형,하진일,주동현,염동훈,김두영 동아대학교 정보기술연구소 2002 情報技術硏究所論文誌 Vol.9 No.2

In this paper, We propose the method of initial curve creation in levelset evolution for the edge of objects or the shape extractions. The shape of traditional initial curve is including edge. But we improve the speed of processing to draw many initial curve using special parameter which was solved by binary processing and sequence labeling. In particular, it is necessary to efficiently reduce noise, we use anisotropic diffusion filter. This filter is to reduce blurring that is the fault of traditional filter processing and to preserver an accurate edge part. The levelset method using inward and outward evolution extracts the edge of object drawn initial curve, it performs to calculate shrinking and expanding at the same time.

12-O-Tetradecanoylphorbol-13-Acetate에 의한 HL-60 세포 분화유도중 Vimentin 유전자 전사조절에 대한 AP-1의 역할

임규,김진희,권도원,김승민,이명선,윤경아,손미영,박종일,윤완희,황병두 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

Purpose: To gain insight on the role of AP-1 in transcriptional regulation of vimentin gene during differentiation of HL-60 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA), the levels of vimentin mRNA and AP-1 have been investigated with Northern blot hybridization and DNA mobility shift assay. Materials and Methods: HL-60 cells were grown in RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum and antibiotics in a humidified 5% CO_2 at 37℃. Total RNA was prepared by a modification of the method of Karlinsey et al. Northern blot hybridization was performed by the method of Virca et al. EcoRI fragment of pVIM-GEM was used as probe for vimentin mRNA. DNA mobility shift assay was performed by the method of Lim et al. End labeled DNA probe(Upper strand, 5'-CGCTTGATGAGTCAGCCG- 3') for AP-1 binding activity was mixed with nuclear extracts in a 20 μl reaction volume containing 300 mM KCI, 60 mM HEPES, pH 7.9, 25 mM MgCI_2, 1 mM EDTA, 1 mM DTT, 60% glycerol, and 2 ㎍ of poly[dI-dC]. Results: TPA increased vimentin mRNA levels, with maximal stimulation reached at 24 hr. The level of vimentin mRNA was induced in proportion to the concentration of TPA. TPA-induced vimentin mRNA was almost reduced by actinomycin-D pretreatment. TPA-induced stimulation of vimentin gene was completely reduced by staurosporin pretreatment. In DNA mobility shift assay, AP-1 newly appeared at 24 hr during TPA-induced differentiation and was almost not detected after the pretreatment of staurosporin. Conclusions: These results suggest that the induction of vimentin mRNA during TPA-dependent differentiation in HL-60 cells may be mediated by protein kinases C signal transduction and AP-1 is important to transcriptional regulation.