강릉연안 홍조 긴잎돌김(Porphyra pseudolinearis Ueda)의 원형질체 분리

김형근,김영대,전방욱 강릉대학교 동해안지역연구소 2000 東海岸硏究 Vol.11 No.2

Two kinds of enzymes solution, capable of degrading the cell wall of red alga Porphyra psuedolinearis Ueda, were prepared from the abalone acetone powder and alkaline hemicellulase. Selected vegetative fronds(3cm) were washed thoroughly in sterile seawater. These blade pieces was kept overnight in an antibiotic solution, and used for isolated protoplast. The vegetative tissue was pretreated, cut into small pieces and soaked in 10% papain solution, and then the small pieces of tissue were incubated in tube containing 5ml of 10% abalone aceton powder solution and 0.01% alkaline hemicellulase solution(MES buffer and pH 6.0) respectively. Protoplasts were removed from the enzyme solution by filtration through a 40μm nylon mesh followed by centrifuge. Yields of protoplasts was higher in alkalin hemicellulase solution(6.0×106/0.1g) than in abalone acetone powder solution(5.4×105/0.1g).

도암호의 수화현상과 환경요인분석

전방욱,김형섭,김형근 강릉대학교동해안지역연구소 1993 東海岸硏究 Vol.4 No.3

수화를 일으키는 원인종을 찾고, 수화에 미치는 각각의 환경요인의 영향을 밝히기 위하여 요인분석을 시도하였다. 수화를 일으키는 원인종은 Microcystis aeruginosa로 밝혀졌다. 제1요인은 식물성 플랑크톤에 의한 수화와 관계가 있었는데 이는 엽록소 a에 대한 인자부하량이 +0.93으로 나타났기 때문이다. 식물성 플랑크톤은 호소표층수에서 인을 소모시키는 것으로 나타났으며 pH, 부유물질량, 탁도등을 증가시켰다. 제2요인은 유기오염과 관계가 있었는데 이는 생물화학적 산소요구량과 대장균군수의 인자부하량이 각각 +0.68과 +0.78로 나타났기 때문이다. The water bloom forming species were investigated, and factor analysis was applied to scrutinize the influence of different environmental factor on water blooming. The water bloom forming species was identified as Microcystis aeruginosa. The first factor was phytoplankton blooming, because it has positive loadings for chlorophy11 a (+0.93). It showed that phytoplankton cause deprivation of phosphorus from lake surface water and increases of pH, suspended solid and turbidity. The second factor was organic pollution because it has positive loadings for biochemical oxygen demand (+0.68) and MPN (+0.78).

강릉수력발전소 발전수 방류 전후의 남대천 수질과 미생물 분포

심혜숙,김규중,김형섭,최창근,전방욱 江陵大學校東海岸地域硏究所 1993 東海岸硏究 Vol.4 No.1

강릉수력발전소의 발전수 방류가 강릉 남대천의 수질 및 미생물 분포에 미치는 영향을 조사하였다. 정점 3 상류지역의 발전수 방류는 수질에 상당한 영향을 끼쳐 정점 3 및 정점 4의 수질은 악화되고 정점 5, 정점 6 및 정점 7의 수질은 개선되었다. 미생물 분포도 이와 유사하여 정점 3 및 정점 4의 세포수는 상당히 증가한 반면 정점 5, 정점 6 및 정점 7에서는 대체적으로 감소하였다. 이 결과로 미루어보아 강릉수력발전소의 발전수는 희석효과에 의해 수질 및 미생물 분포를 변화시키는 것으로 사료된다. The effects of water discharged from Kangnung hydropower plant on the water quality and some bacterial population of Namdaecheon Stream, Kangnung were investigated. The water discharged above station 3 affected the water quality significantly; the water quality of station 3 and station 4 took a turn for the worse, however those of station 5, station 6 and station 7 changed for the better. It was also true with the bacterial population; The number of cells of bacteria increased in station 3 and 4, but the number significantly decreased in station 5, station 6, and station 7. The data suggested the idea that the water discharged from Kangnung hydropower plant changed the water quality and some bacterial population by dilution effect.

영동지역 4개 저수지의 식물성 플랑크톤 분포에 의한 오수생물학적 연구

박영제,김양배,전방욱,김형섭 江陵大學校東海岸地域硏究所 1993 東海岸硏究 Vol.4 No.1

영동지역에 위치하는 4개 저수지를 대상으로 1991년 9월에 수질조사 및 식물성 플랑크톤 분포에 의한 오수생물학적 수질평가 연구를 시도하였다. 수질평가를 위하여 수온, 수소이온농도, 총질소, 총인, 엽록소 a, 및 식물성 플랑크톤의 현존량을 조사하였다. 동정된 식물성 플랑크톤은 총 58종류로 남조류 10종, 황색편모조 1종, 와편모조 2종, 갈색편모조 1종 및 녹조류 37종류였다. 식물성 플랑크톤의 우점종은 저수지별로 달랐으며, 죽헌저수지는 Microsystis aeruginosa, 북동저수지는 Peridinium cunningtonii var. contactum, 달방댐은 Staurastrum simonyi var. simonyi, 초당저수지는 Chlamydomonas umbonata로 밝혀졌다. 수질 및 식물성 플랑크톤의 분포유형을 기준으로 호수 수질을 생물학적으로 판정할 때 죽헌, 북동 및 초당저수지는 β-중부수성 호수로 달방댐은 빈부수성 호수로 판정되었다. The survey of water quality and saprobiotic phytoplankton distribution was carried out in 4 reservoirs in eastern area of Kangwon Province at September 1991. Temperature, pH, total-N, total-P, chl.a, and standing crop of phytoplankton were measured for water quality examination. Total numbers of phytoplankton identified were 58 spp, consisted of 10 spp Cyanophyta, 1 spp Chrysophyta, 2 spp Dinophyta, 1 spp Crytophyta, and 37 spp Chlorophyta. The dominant species of phytoplankton in each reservoir was different as follows: Microsystis aeruginosa in Jukhean, Peridinium cunningtonii var. contactum in Bukdong, Staurastrum simonyi var. simonyi in Dalbang, and Chlamydomonas umbonata in Chodang. Estimated from the data of water quality and phytoplankton distribution, the reservoirs of Jukheon, Bukdong and Chodang were β-mesosaprobic whereas Dalbang was oligosaprobic.

Targeting cytokines and signaling molecules related to immune pathways in atopic dermatitis: therapeutic implications and challenges

Hyung-Ook Kim 대한약학회 2022 Archives of Pharmacal Research Vol.45 No.12

Although atopic dermatitis (AD) is primarily aTh2-driven disease, it shows high heterogeneity with additionalvariable contributions of the Th22, Th17, and Th1pathways, depending on the subtype of the disease. Expandingknowledge and understanding of AD pathogenesis haspromoted the development of numerous novel therapeuticsthat target cytokines and their signaling molecules, representatively,Janus kinases, involved in the underlyingimmune pathways, resulting in therapeutic success and failure. The fi rst FDA approval was for the targeted biologicdupilumab. Although this proved the therapeutic relevanceof targeting Th2 cytokines in moderate-to-severe forms ofAD, it did not treat all patients, necessitating additional targetedtherapeutics that modulate other cytokine pathwaysto resolve AD in all subtypes. Three more recently FDAapprovedtargeted therapeutics and several others that havebeen developed represent different targeted approachesdirected to the Th2, Th22, Th17, or Th1 pathways. Thisreview summarizes the main features and clinical outcomesof various approaches targeting cytokines and signaling moleculesin these diff erent pathways in view of both successfuland failed cases, with a discussion of their therapeutic implications. In future, AD should be treated with more specifi ctreatments refl ecting the disease heterogeneity, but the currentdevelopment of targeted therapeutics has faced somechallenges in this context, which is also discussed.

Development of JAK inhibitors for the treatment of immune‑mediated diseases: kinase‑targeted inhibitors and pseudokinase‑targeted inhibitors

Hyung‑Ook Kim 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.11

JAKs are a family of intracellular tyrosinekinases consisting of four members, JAK1, JAK2, JAK3,and TYK2. They are key components of the JAK-STATpathway that transmit signals of many cytokines involvedin the pathogenesis of numerous immune-mediated diseasesand have been major molecular targets in developing newdrugs for the treatment of such diseases. Some small-moleculeinhibitors of JAKs have been approved by the FDA forrheumatoid arthritis, psoriatic arthritis, and inflammatorybowel disease. Now, newer JAK inhibitors with isoformselectivityamong the four different JAKs are being developed,with the aim of improving clinical outcomes comparedwith earlier developed drugs with pan-JAK inhibition. Mostof these selective inhibitors target the kinase domains ofJAKs, functioning through the traditional inhibition modeof kinases; but recently those that target their pseudokinasedomains, allosterically inhibiting the enzymes, have beenunder development. In this review, key characteristics, efficacy,and safety of FDA-approved and representative drugsin late stages of development are briefly described in orderto provide clinical implications with respect to JAK inhibitorselectivity and future development perspectives. The recentdevelopment of pseudokinase-targeted inhibitors of JAKsis also included.

Development of BTK inhibitors for the treatment of B-cell malignancies

Hyung-Ook Kim 대한약학회 2019 Archives of Pharmacal Research Vol.42 No.2

BTK is a key component of B-cell receptor signaling and functions as an important regulator of cell proliferation and survival in B-cell malignancies. The first-in-class BTK inhibitor ibrutinib is a small molecule drug that binds covalently to BTK and has been proved to be an effective treatment for various B-cell malignancies. However, it has off-target activities on non-BTK kinases that are related to side effects or might be translated into clinical limitations, with resistance to ibrutinib also reported. Much progress has been made in the development of more selective and second-generation BTK inhibitors. A recent shift in the mechanisms of action of BTK inhibitors is noteworthy, and novel inhibitors acting through noncovalent BTK inhibition are now being developed. This review describes key characteristics of ibrutinib, including current issues of its clinical use, and summarizes preclinical properties and clinical developments of second-generation BTK inhibitors for the treatment of B-cell malignancies. A review of novel noncovalent BTK inhibitors are also included.

Cetuximab plus Irinotecan in Metastatic Colorectal Cancer

Hyung Ook Kim,Hungdai Kim,Wou Kon Han 대한종양외과학회 2008 대한임상종양학회 학술대회지 Vol.2008 No.5

Oral Presentation : Long-term survival with Gemcitabine alone chemotherapy for Locally-advanced non-metastatic pancreatic cancer: Report of one case (초)

Hyung Ook Kim,Hung Dai Kim,Jun Ho Shin 대한임상종양학회 2009 대한임상종양연구 심포지엄 Vol.18 No.-

Long-term survival with Gemcitabine alone chemotherapy for locally-advanced non-metastatic pancreatic cancer

Hyung Ook Kim,Hungdai Kim,Jun Ho Shin 대한종양외과학회 2009 대한임상종양학회 학술대회지 Vol.2009 No.3