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방사선에 의해 제조된 pH 및 온도에 민감한 수화겔의 물성연구 및 약물방출에 관한 연구
김현우,노영창,손태일 한국공업화학회 2003 공업화학 Vol.14 No.3
N-isopropylacrylamide (NIPAAm)와 acrylic acid (AA)를 증류수에 일정한 몰비로 용해한 것과 여기에 butyl acrylate (BA)를 혼합한 용액을 상온에서 30, 50 kGy로 γ-선 조사하여 중합반응과 가교를 동시에 일으켜 pH 및 온도에 민감한 수화겔을 제조하였다. 제조된 수화겔은 pH 2, 7의 용액에서 5~40℃로 온도를 변화시켜 각각의 온도에서 최대 팽윤도를 측정하고 한편으로는 37℃로 고정한 후 PH 2~8까지 pH에 대한 최대 팽윤도를 측정하였다. 수화겔의 팽윤과 수축에 이온강도가 어떤 영향을 미치는지 알아보기 위해 0, 0.01, 0.03 N의 염화나트륨 용액에서 팽윤실험을 하였다. 경구용 인슐린 전달제재를 제조할 목적으로 수화겔이 최대 팽윤할 수 있는 최저의 온도와 pH 조건에서 인슐린을 탑재한 다음 자연건조(air dried)와 동결건조(freeze dried)를 하여 인슐린을 함유한 겔(drug-gels)을 제조하였으며, 인슐린 탑재 후 남은 인슐린 용액으로부터 탑재효율을 구하였다. Drug-gel은 in vitro에서 인공위액(simulated gastric fluid; SGF)과 인공장액(simulated intestinal fluid ; SIF)에서 약물 방출량을 측정하여 방출속도를 측정하였다. Temperature and pH sensitive N-isopropyacrylamide-acrylic acid (NIPAAm-AA) and N-isopropylacrylamide-acrylic acid-butyl acrylate (NIPAAm-AA-BA) hydrogels were synthesized by simultaneous polymerization and crosslinking of NIPAAm AA, and BA monomers in aqueous solution using γ-rays from ^60Co source at room temperature. The measurements of equilibrium water content on the synthesized hydrogels were made at the temperature ranging from 5 to 40℃ and pH 2 and 7. The prepared hydrogels had temperature-sensitive (30~35℃) and pH-sensitive swelling behavior. The HIPAAm-AA and NIPAAm-AA-BA hydrogels were used in drug delivery systems for the controlled release of insulin Insulin has been shown to have binding sites for hydrophobic molecules. Thus, it is likely that insulin binds specifically to the butyl side. The gels including insulin were prepared by air drying or freeze drying after loading insulin in solution (14.1 IU/mL) at 5℃ and pH 7. Drug-gels were plated in simulated gastric fluid (SGF; pH 1.2) at 37℃ for 2 h and then at simulated intestinal fluid (SIF : pH 7.3). The insulin release of the drug-gels was examined by UY analysis.
Nho, Jin-Woo,Hwang, In-Guk,Kim, Hyun-Young,Lee, Youn-Ri,Woo, Koan-Sik,Hwang, Bang-Yeon,Chang, Seong-Jun,Lee, Jun-Soo,Jeong, Heon-Sang 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.2
The objectives of this study were to evaluate the antioxidant activity, angiotensin I-converting enzyme (ACE) inhibitory activity, and anticancer activity of ramie (Boehmeria nivea) leaves (RL). The RL was extracted with 70%(v/v) ethanol (RLE) and fractionated with the solvents of hexane, chloroform, ethylacetate, and aqueous. The ethylacetate fraction (EF) contained the highest phenolic contents of 651.65 mg/g, followed by RLE of 148.72 mg/g. The EF showed strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, superoxide radical scavenging activity, and hydroxyl radical scavenging with a 50% inhibition concentration ($IC_{50}$) of 0.097, 0.129, and 0.191 mg/mL, respectively. The ACE-inhibitory activity of EF was 80.32% at a concentration of 0.1 mg/mL. The EF showed growth-inhibitory effect of 67.21% at 0.25 mg/mL on the LoVo cell line, and 56.08% at 0.25 mg/mL on the NCI-H460 cell line, respectively. Therefore, it was suggested that the RL were potential materials for use as functional food and medicine.
Nho, Hyun Woo,Yang, Nuri,Song, Jaewoo,Park, Joon Shik,Yoon, Tae Hyun Elsevier 2017 Sensors and actuators. B Chemical Vol.249 No.-
<P><B>Abstract</B></P> <P>Shape-based separation capabilities of a novel pinched flow fractionation device with a tilted sidewall and vertical focusing channels (t-PFF-v) were demonstrated for (1) spherical and disc-shaped polystyrene (PS) particles; (2) platelets (PLT) and red blood cells (RBC); and (3) singlet, doublet, and triplet clusters of disc-shaped PS particles. The tilted sidewalls and vertical focusing channels of the t-PFF-v device allowed us to perform enhanced separation of non-spherical particles. Using this t-PFF-v device with a W<SUB>p</SUB> (pinched segment width) of 20μm and 15μm, not only spherical (diameter of 2μm) and disc-shaped (thickness of ∼2μm, diameter of ∼5.0μm) PS particles but also PLTs and RBCs from diluted blood were well separated with good separation resolutions (R<SUB>disc,sphere</SUB> =1.40 and R<SUB>PLT,RBC</SUB> =1.28). Additionally, time-lapse images of flowing particles indicated that both disc-shaped PS particles and RBCs are probably having type I orientation in the pinched segment (i.e., leaning against the tilted sidewalls at the pinched segment). Moreover, we observed streamlines of singlet, doublet, and triplet clustered disc-shaped PS particles, which suggested that these clustered particles could be well separated according to their aspect ratio. The doublet or triplet clustered PS particles are probably lay on the floor and rotated at the pinched segment (type II orientation). We believe that this shape-based separation capability achieved by the t-PFF-v device can be utilized for many application areas, such as medical, biological, material and colloidal sciences.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Shape-based separation capability of t-PFF-v device was demonstrated for spherical and disc-shaped PS particles. </LI> <LI> By using t-PFF-v device, blood components such as platelets (PLTs) and red blood cells (RBCs) were separated. </LI> <LI> Singlet, doublet, and triplet clusters of disc-shaped PS particles were also separated by using t-PFF-v device. </LI> <LI> The tilted sidewalls and vertical focusing channels of the t-PFF-v device allowed us to perform enhanced separation of non-spherical particles. </LI> <LI> Time-lapse images suggest that both disc-shaped PS particles and RBCs are leaning against the tilted sidewalls at the pinched segment. </LI> </UL> </P>
Beneficial Effects of Cynaroside on Cisplatin-Induced Kidney Injury In Vitro and In Vivo
Nho, Jong-Hyun,Jung, Ho-Kyung,Lee, Mu-Jin,Jang, Ji-Hun,Sim, Mi-Ok,Jeong, Da-Eun,Cho, Hyun-Woo,Kim, Jong-Choon Korean Society of ToxicologyKorea Environmental Mu 2018 Toxicological Research Vol.34 No.2
Anti-cancer drugs such as cisplatin and doxorubicin are effectively used more than radiotherapy. Cisplatin is a chemotherapeutic drug, used for treatment of various forms of cancer. However, it has side effects such as ototoxicity and nephrotoxicity. Cisplatin-induced nephrotoxicity increases tubular damage and renal dysfunction. Consequently, we investigated the beneficial effect of cynaroside on cisplatin-induced kidney injury using HK-2 cell (human proximal tubule cell line) and an animal model. Results indicated that $10{\mu}M$ cynaroside diminished cisplatin-induced apoptosis, mitochondrial dysfunction and caspase-3 activation, cisplatin-induced upregulation of caspase-3/MST-1 pathway decreased by treatment of cynaroside in HK-2 cells. To confirm the effect of cynaroside on cisplatin-induced kidney injury in vivo, we used cisplatin exposure animal model (20 mg/kg, balb/c mice, i.p., once a day for 3 days). Renal dysfunction, tubular damage and neutrophilia induced by cisplatin injection were decreased by cynaroside (10 mg/kg, i.p., once a day for 3 days). Results indicated that cynaroside decreased cisplatin-induced kidney injury in vitro and in vivo, and it could be used for improving cisplatin-induced side effects. However, further experiments are required regarding toxicity by high dose cynaroside and caspase-3/MST-1-linked signal transduction in the animal model.
Nho, Hyun Woo,Park, Joon Shik,Yoon, Tae Hyun IOP 2017 Journal of micromechanics and microengineering Vol.27 No.5
<P>A pinched flow fractionation device with trapezoid-shaped pinched segment and cross-flow side channels (t-PFF-v) was redesigned and fabricated with a one-stop anisotropic chemical wet etching process and its enhanced separation capability was demonstrated for spherical polystyrene (PS) particles with diameter ranges of 2–8 <I>µ</I>m. The tilted sidewalls and vertical focusing channels of the t-PFF-v device led to an improved separation resolution (<I>R</I> <SUB> <I>m</I>,<I>n</I> </SUB>) via enhanced separation distance of PS particles at the pinched segment (Δ<I>x</I> <SUB> <I>m</I>,<I>n</I> </SUB>) and reduced effluent particle distribution width (<I>s</I> <SUB> <I>n</I> </SUB>). Using this t-PFF-v device with <I>W</I> <SUB>p</SUB> (pinched segment width) of 25 <I>µ</I>m, PS particles with diameters of 4, 6, and 8 <I>µ</I>m were simultaneously separated with good separation resolution (<I>R</I> <SUB>4,6</SUB> = 3.2 and <I>R</I> <SUB>6,8</SUB> = 3.4). Moreover, by adapting even smaller pinched segment width (<I>W</I> <SUB>p</SUB> = 15 <I>µ</I>m), PS particles with diameters of 2 and 4 <I>µ</I>m, not well separated in the previous studies, were clearly separated with 9.5-fold improvement in <I>R</I> <SUB>2,4</SUB> compared with the normal PFF device (n-PFF). The effects of inlet flow rate ratio (<I>Q</I> <SUB>2</SUB>/<I>Q</I> <SUB>1</SUB>) on the separation efficiency were also carefully investigated and provided additional insight on the separation processes within the pinched segment of t-PFF-v device. The drain flow through the cross-flow v-channels helps better alignment of particles at the sidewall of pinched segment and resulted in significantly reduced distribution of effluent particles, even under the operating conditions with low <I>Q</I> <SUB>2</SUB>/<I>Q</I> <SUB>1</SUB> values.</P>
Beneficial Effects of Cynaroside on Cisplatin-Induced Kidney Injury In Vitro and In Vivo
Jong-Hyun Nho,Ho-Kyung Jung,Mu-Jin Lee,Ji-Hun Jang,Mi-Ok Sim,Da-Eun Jeong,Hyun-Woo Cho,Jong-Choon Kim 한국독성학회 2018 Toxicological Research Vol.34 No.2
Anti-cancer drugs such as cisplatin and doxorubicin are effectively used more than radiotherapy. Cisplatin is a chemotherapeutic drug, used for treatment of various forms of cancer. However, it has side effects such as ototoxicity and nephrotoxicity. Cisplatin-induced nephrotoxicity increases tubular damage and renal dysfunction. Consequently, we investigated the beneficial effect of cynaroside on cisplatin-induced kidney injury using HK-2 cell (human proximal tubule cell line) and an animal model. Results indicated that 10 μM cynaroside diminished cisplatin-induced apoptosis, mitochondrial dysfunction and caspase-3 activation, cisplatin-induced upregulation of caspase-3/MST-1 pathway decreased by treatment of cynaroside in HK-2 cells. To confirm the effect of cynaroside on cisplatin-induced kidney injury in vivo, we used cisplatin exposure animal model (20 mg/kg, balb/c mice, i.p., once a day for 3 days). Renal dysfunction, tubular damage and neutrophilia induced by cisplatin injection were decreased by cynaroside (10 mg/kg, i.p., once a day for 3 days). Results indicated that cynaroside decreased cisplatin-induced kidney injury in vitro and in vivo, and it could be used for improving cisplatin-induced side effects. However, further experiments are required regarding toxicity by high dose cynaroside and caspase-3/MST-1-linked signal transduction in the animal model.
( Ji-hun Jang ),( Ki-ho Lee ),( Jong-hyun Nho ),( Hyun-joo Lee ),( Beo-dul Yang ),( Ho Park ),( Hyun-woo Cho ),( Byeong-kwan An ),( Sun-ra Kim ),( Ju-hyun Yong ),( Ro-dong Park ),( Ho-kyung Jung ) 한국응용생명화학회 2020 Journal of Applied Biological Chemistry (J. Appl. Vol.63 No.4
We previously reported the potential of Senna tora L. seeds fermented by Lactobacillus casei (FSL) as a laxative agent in a loperamide-induced constipation rat model. Here, we examine the mechanism of action of FSL and its bioactive compound, revealed herein, on loperamide-induced constipation Sprague Dawley rat model. We identified the compound aurantio-obtusin (AO) using HPLC quantitative analysis. Rats were randomly assigned to six experimental groups (eight rats each)-normal and constipated groups (loperamide, FSL [100, 300, 500 mg/kg], and AO [1 mg/kg]). The FSL and AO-treated group showed an increase in the frequency, amount, and water content of feces in the constipated rat. Moreover, FSL and AO increased the intestinal transit speed in the constipated rat. Histological analysis revealed that FSL and AO recovered the intestinal mucus, the number of goblet cells, as well as thickness of the mucosa layer and muscle. Furthermore, the protein levels of the muscarinic acetylcholine receptor M3, which is involved in intestine contraction, were recovered in the FSL and AO-treated group. Its downstream signaling pathway (p-protein kinase C) was recovered by FSL and AO treatment. In conclusion, fermentation of S. tora L. seeds increases AO, which improves intestinal function, indicating that FSL is effective for treating constipation.