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쑥갓(Chrysanthemum coronarium L.)으로부터 배당체의 분리
송명종,김세영,송효남,백남인 경희대학교 생명자원과학연구원 2004 硏究論文集 Vol.23 No.-
쑥갓의 지상부를 MeOH로 추출하고, 추출물을 EtOAc, n-BuOH 및 물로 분배, 추출하였다. n-BuOH분획으로부터 silica gel 및 ODS column chromatography를 반복하여 배당체를 분리하였다. 화합물의 화학구조는, MNR 및 MS 등의 스펙트럼을 해석하여 1-o-β-D-glucopyranosyl-3-hexenol으로 동정하였다. The aerial parts of Garland (Chrysanthemum coronarium L.) were extracted in MeOH and solvent fractionated with EtOAc, n-BuOH and water, successively. n-BuOH fraction gave a glycoside compound through application of silica gel column chromatographies including ODS. The chemical structure of the glycoside was determined by the interpretation of several spectral data, including NMR and MS as O-β-D-glucopyranosyl-3-hexenol (1).
자연과 수업에서 Vee Diagram 의 적용에 대한 연구
정진우,송보용,김효남 한국교원대학교 과학교육연구소 1994 청람과학교육연구논총 Vol.4 No.1
The purpose of this study is to analize the effect of Vee Diagram on the ability of scientific investigation, scientific attitude, cognitive ability in elementary school. For this study, 68 sixth grade students were selected and they were divided into two group: one is the experimenal group with Vee diagram instruction, the other is the control group with conventional instruction. Data were collected from the samples to know effect of Vee Diagram instruction and compared the experimental group with the control group. The result of analysis is as follows: 1. The vee diagram instruction is better than the conventional instruction for improving the scientific inquiry skills. 2. The vee diagram instruction does not show statistically meaningful difference to the conventional instruction for improving the scientific attitude. 3. The vee diagram instruction is better than conventional instuction for improving scientific knowledge. The results indicated that Vee diagram instruction is effective in learning strategy for improving scientific attitude.
Hyo Song Nam,Ane J. Anderson,Young Cheol Kim 전남대학교 농업과학기술연구소 2021 농업생명과학연구동향 Vol.59 No.-
Plant health can be affected positively and negatively by microbial volatiles. In this study, we examined the effect of the growth medium for Pseudomonas chlororaphis 06, a probiotic root coonizer, on the production of volatiles that affect the growth of Arabidopsis thaliana. In an I-plate bioassay, volatiles from the wild-type P. chlororaphis cells grown on nutrient agar significantly inhibited the shoot and root growth of A. thaliana. Volatiles from the wild-type P. chlororaphis cells grown on Methyl Red Vogues Proskauer medium (MR-VP) agar increased root branching with a minor reduction in root elogation. These responses were absent with exposure to volatile hydrogen cyanide(HCN). HCN synthesis was terminated in one mutant via delection of HCN-biosynthetic gene, wheareas the second mutant lacked expression of the global regulator GacS. The volatiles of the wild-type P. chlororaphis of the gaS mutant grown on MR-VP agar increased the shoot mass. However, the responses differed for mutants of A. thaliana—one lacked the ethylene receptor, another lacked sensitivity to jasmonic acid, and the third was unable to accumulate salicyli acid. These results indicate that the production of volatiles is significantly affected by the growth media utilized for P. chlororapjos 06 and that their effects on plant growth are infulenced by plant defense signaling pathways.
Impact of Various Factors on Color Stability of Fresh Blueberry Juice during Storage
Hyo-Nam Song,So-Ae Ji,Hye-Rim Park,Ho-Hyoen Kim,Christer Hogstrand 한국식품영양과학회 2018 Preventive Nutrition and Food Science Vol.23 No.1
The attractive purple color of blueberries (Vaccinium spp.) is unstable and susceptible to degradation during food processing and storage. The effects of various factors on the color stability of fresh blueberry juice were investigated. Total soluble solid content, pH, and total anthocyanin content were measured. Heating at 30℃ and 60℃ for 300 min did not influence the color stability, but heating at 100℃ drastically decreased it by 33.0%. Sugars decreased color in a concentration-dependent manner. However, glucose and galactose had significantly protective effects on the color disruption than fructose, maltose, and sucrose. Organic acids lowered the color intensity in the order of citric acid〉 tartaric acid〉 malic acid〉 formic acid〉 acetic acid during 10 days of storage. Color decreased faster during long-term light exposure than in the dark. The color in the dark was kept by 58.7% after 7 weeks, while 48.9% in the light. Color retention was significantly decreased to 93.5% and 93.8% at 4℃ and -20℃, respectively, after 7 weeks, while 95.40% at -75℃. We suggest that blueberry juice color can be protected by keeping the extraction temperature below 60℃ with the selective addition of glucose, galactose, or citric acid. For long-term storage, it is recommended to use a light-protected container and a deep freezer at -75℃.
Nam, Hyo Song,Anderson, Anne J.,Kim, Young Cheol The Korean Society of Plant Pathology 2018 Plant Pathology Journal Vol.34 No.3
Commercial biocontrol of microbial plant diseases and plant pests, such as nematodes, requires field-effective formulations. The isolate Pseudomonas chlororaphis O6 is a Gram-negative bacterium that controls microbial plant pathogens both directly and indirectly. This bacterium also has nematocidal activity. In this study, we report on the efficacy of a wettable powder-type formulation of P. chlororaphis O6. Culturable bacteria in the formulated product were retained at above $1{\times}10^8$ colony forming units/g after storage of the powder at $25^{\circ}C$ for six months. Foliar application of the diluted formulated product controlled leaf blight and gray mold in tomato. The product also displayed preventative and curative controls for root-knot nematode (Meloidogyne spp.) in tomato. Under laboratory conditions and for commercially grown melon, the control was at levels comparable to that of a standard commercial chemical nematicide. The results indicated that the wettable powder formulation product of P. chlororaphis O6 can be used for control of plant microbial pathogens and root-knot nematodes.
Functional Cordyceps Coffee Containing Cordycepin and β-Glucan
Hyo-Nam Song 한국식품영양과학회 2020 Preventive Nutrition and Food Science Vol.25 No.2
Cordyceps coffee, containing Cordycepin and β-glucan, was developed to improve quality and functionality of coffee. We evaluated its biological activities and quality characteristics. We treated green coffee beans with mixed extracts from three medicinal mushrooms, Cordyceps mushroom (Cordyceps militaris), Phellinus mushroom (Phellinus linteus), and Chaga mushroom (Inonotus obliquus). Both control [27.00 ㎎ gallic acid equivalent (GAE)/g] and Cordyceps coffee (37.34 ㎎ GAE/g) had higher contents of polyphenol than the Cordyceps mushroom raw materials (23.17 ㎎ GAE/g). 1,1-Diphenyl-2-picrylhydrazyl scavenging activity was higher for both the control and Cordyceps coffee (80.56% and 82.21 %, respectively) compared with Cordyceps mushrooms (62.89%). β-Glucan content was determined by the enzyme method; the raw Cordyceps, Phellinus, and Chaga mushrooms contained 3.79%, 7.06%, and 8.57% β-glucan, respectively. However, β-glucan and total glucan contents were much lower in Cordyceps coffee (2.03% and 3.11%, respectively) than in the raw mushrooms. The amount of Cordycepin, as determined by high performance liquid chromatograph, was 2,274.70 ㎎/㎏ for the Cordyceps coffee and 11,533.22 ㎎/㎏ for the Cordyceps mushrooms. Through flavor pattern analysis using the electronic nose system, we showed Cordyceps coffee kept the original coffee aroma (similar as the control), and this was not obstructed by the off-flavor of the Cordyceps mushrooms. In conclusion, this study suggests that Cordyceps coffee may be a novel functional coffee containing the functional components Cordycepin and β-glucan.