Antimicrobial Susceptibility and Characterization of Propionibacterium acnes by Multilocus Sequence Typing and Repetitive-Sequence-Based PCR

Kim, Shukho,Song, Hyesoon,Lee, Weon-Ju,Kim, Jungmin The Korean Society for Microbiology 2016 Journal of Bacteriology and Virology Vol.46 No.3

Propionibacterium acnes, a gram-positive, anaerobic, and aerotolerant bacterium that is found frequently in the skin as part of the human microbiome causes inflammatory acne, shoulder infection, and the contamination of medical devices. The study goals were the antibiotic resistant and molecular epidemiological characterization of the P. acnes isolates in Korea. A total of 22 P. acnes isolates originated from diverse patients were obtained from three National Culture Collections for Pathogens in South Korea. The hemolytic properties and minimum inhibitory concentrations (MIC) of five antibiotics (tetracycline, doxycycline, clindamycin, erythromycin, and minocycline) were determined. Only one isolate showed high MIC values and resistance to all five antibiotics. Genotypic characterization was achieved by multilocus sequence typing (MLST) for eight loci (aroE, guaA, tly, camp2, atpD, gmk, lepA, and sodA) and repetitive-sequence-based PCR (rep-PCR) analysis using the DiversiLab kit. MLST revealed four phylogroups that were type $IA_1$ (27.3%), type $IA_2$ (18.2%), type IB (13.6%), and type II (40.9%). Rep-PCR results demonstrated three clusters that were cluster I (39.1%), cluster II (45.5%), and cluster III (13.6%). The isolates of cluster I were part of phylogroup type IA (both $IA_1$ and $IA_2$), and the isolates of cluster II belonged to phylogroup type II. All isolates of phylogroup type IB were hemolytic and belonged to cluster III. The results of rep-PCR clustering analysis showed a good correlation with those of MLST phylogroups, suggesting that rep-PCR could be an alternative method to track P. acnes subtype lineages.

Affective Factors That Contribute to the Quality of Life of Juvenile Inmates with Attention-Deficit/Hyperactivity Disorder : A Focus on Items from the Korean Youth Self Report

Hyesoon Kim,Bongseog Kim 대한소아청소년정신의학회 2019 소아청소년정신의학 Vol.30 No.4

Objectives: This study investigated quality of life in Korean juvenile inmates with attention-deficit/hyperactivity disorder (ADHD) and the impact of behavioral and emotional problems on quality of life. Methods: In total, 200 inmates were evaluated using the Korean version of the Mini-International Neuropsychiatric Interview (KMINI) and the Korean version of the Kiddie-Schedule for Affective Disorders and Schizophrenia-Present and Lifetime (K-SADS-PL-K). We extracted the inmates with ADHD and evaluated their quality of life, behavioral problems, and emotional problems with the Pediatric Quality of Life Inventory (PedsQL) and the Korean Youth Self Report (K-YSR) scale. Descriptive statistics, Pearson correlation analysis, and multiple regression analysis were conducted. Results: Among the 200 total inmates, 68 were diagnosed with ADHD by the K-SADS-PL-K. Most of the correlations between PedsQL scores and K-YSR items were significant. Multiple regression analysis showed that PedsQL could be predicted by affective problems (among the DSM-oriented scales of the K-YSR) and attention problems (among the syndrome scales of the K-YSR). Conclusion: Our results demonstrate that, among juvenile inmates with ADHD, quality of life was negatively correlated with most behavioral and emotional problems. Meanwhile, the significant influence of affective and attention problems on inmates’ quality of life suggests the necessity of comprehensive treatments for this group.

Carrot Heat Shock Protein DcHsp17.7 is Present in Various Tissues without Thermal Stress and is Regulated by Tissue Type and Thermal Stress

Hyesoon Kim,Yeh-Jin Ahn 한국원예학회 2010 Horticulture, Environment, and Biotechnology Vol.51 No.3

DcHsp17.7 is a heat-inducible small heat shock protein (sHSP) in carrot (Daucus carota L.). Moreover, here we report that DcHsp17.7 is constitutively expressed in vegetative and reproductive tissues at defined developmental stages. Immunoblot analysis using a polyclonal antibody raised against DcHsp17.7 showed that the protein was present in non-stressed leaf tissue and gradually accumulated upon heat stress (40℃). In the heat-induced level of DcHsp17.7, there were transient, but reproducible declines, suggesting dynamic expression regulation. The protein was also present in dry and germinating seeds. It is worth noting that unlike most other seed sHSPs, which disappear upon completion of germination, DcHsp17.7 was continuously present in young seedling. In flower bud and open flower, the protein was constitutively expressed. Heat stress increased the amount of DcHsp17.7 in these floral tissues. Under the opposite temperature extreme, cold stress (2℃), DcHsp17.7 was also present in these tissues, but the levels were lower than those under heat stress. Constitutive expression of DcHsp17.7 suggests that it may play physiological role(s) in the absence of thermal stress. Alternatively, DcHsp17.7 in non-stressed tissues may confer advanced protection to sudden temperature changes by immediately enhancing thermotolerance of cells.

유아의 인지능력, 어머니의 양육행동, 어머니와 교사에 대한 애착이 유아의 만족지연능력에 미치는 영향

김혜순(Hyesoon Kim) 한국생활과학회 2005 한국생활과학회지 Vol.14 No.5

Antimicrobial Susceptibility and Characterization of Propionibacterium acnes by Multilocus Sequence Typing and Repetitive-Sequence-Based PCR

Shukho Kim,Hyesoon Song,Weon-Ju Lee,Jungmin Kim 대한미생물학회 2016 Journal of Bacteriology and Virology Vol.46 No.3

광역산소 센서를 이용한 실린더별 공연비 제어

김혜순(Hyesoon Kim),박진일(Jinil Park),고상근(S.Ken Kauh) 한국자동차공학회 1998 한국자동차공학회 춘 추계 학술대회 논문집 Vol.1998 No.5_1

There are about ±3% A/F ratio differences among cylinders for a normal gasoline engine. These differences vary with engine operating conditions and are maximized at idle state. To reduce this difference it is necessary to feedback-control the individual cylinder A/F ratio. The purpose of this study is reducing cylinder variation by cylinder A/F control using wide band oxygen sensor.<br/> Wide band oxygen sensor outputs have informations for the individual cylinder A/F ratio. Based on Wide band oxygen sensor's information, cylinder A/F ratio is controlled within ±1% at 1500 rpm and 3000 rpm.<br/>

메이커활동과 연계한 유아 과학놀이 프로그램의 개발 및 효과

김지영 ( Kim Jiyoung ),김혜순 ( Kim Hyesoon ) 미래유아교육학회 2022 미래유아교육학회지 Vol.29 No.4

본 연구는 유아를 위한 메이커활동과 연계한 과학놀이 프로그램의 개발과 그 효과 검증을 목적으로 한다. 이를 위해 먼저 문헌분석 및 선행연구 고찰을 통해 프로그램 구성체계 초안을 세웠다. 이후 3차에 걸친 전문가집단 델파이조사 검증과 프로그램의 현장 예비 적용 단계를 거쳐 프로그램을 개발하였다. 다음으로 G시에 위치한 D유치원의 5세 2개 학급, 유아 56명을 대상으로 본 프로그램의 적용 효과를 검증한 바, 유의하였다. 본 프로그램의 효과 검증 측정도구로는 과학적 탐구능력 검사(김다래, 2018)와 과학적 문제해결력 검사(강경희, 2018), 놀이성 검사(주현정, 2019)가 사용되었다. 결론적으로 본 프로그램은 놀이를 통한 과학적 지식 형성, 또래와의 협력, 자발적 문제해결력 등 유아의 과학적 소양 개발에 기여할 수 있는 내용 타당성을 갖추고 있음을 시사한다. 이에 향후 교육 현장에서 좀 더 널리 활용될 수 있기를 기대한다. This study aimed to develop a science-play program connected to maker-activities for young children and verify its effectiveness. To do this, an initial plan for the program structure was built through literature analysis and a review of prior studies. Afterwards, a program was developed through the three times of Delphi survey verification by a group of experts and a stage of its preliminary application to the field. Next, the effect of the application of the program was verified for 56 early children in two 5-year-old classes in the D kindergarten in G city, and its result was significant. Kim Da-rae’s (2018) young childhood scientific inquiry ability test, Kang Kyung-hee’s (2018) scientific problem-solving ability test, and Joo Hyun-jung’s (2019) playability test were used as measurement tools to verify the effectiveness of the program. In conclusion, the result suggests that the program has content validity that can contribute to the development of scientific literacy of children, such as the formation of scientific knowledge through play, collaboration with peer learners, and voluntary problemsolving ability. Therefore, it is expected that the program can be used widely in early childhood education in the future.

Detection of Bacterial Species in Chronic Periodontitis Tissues at Different Stages of Disease Severity

Da-Le Yoon,Shukho Kim,Hyesoon Song,Yong-Gun Kim,Jae-Mok Lee,Jungmin Kim 대한미생물학회 2015 Journal of Bacteriology and Virology Vol.45 No.4

여자 교수가 당면하는 성차별적 대학일상에 대한 문제제기 : 남녀공학대학을 중심으로

김혜순 한국여성커뮤니케이션학회 2004 미디어, 젠더 & 문화 Vol.2 No.-

최근 우리 사회에서 상위권한층의 여성비율을 증가시키는 데 전략적 근거로 삼고 있는 유엔 여성권한지수의 기본 전제를 배경으로 여자교수에 대한 연구가 필요함을 주장하였다. 여자교수가 경험하는 성차별적 상황을 구체적으로 드러내는 기존연구가 없었던 이유를 생각해보고, 결국 여자교수 스스로가 자신을 주체이자 대상으로 연구에 우선적으로 동원할 수밖에 없음을 논의하였다. 본론으로, 여자교수가 대학교수로서의 일상적 직무수행상 경험했거나 경험할 수 입는 성차별적 사례들을 유형화하면서 기술했다. 필자가 지난 20년 가까이 대학교수로 재직하면서 직접 경험하고 관찰한 것, 동료남녀교수나 연구원을 통한 간접경험에 근거했다. 방법론상 주관적이고 개인적인 해석수준을 넘지 못한다는 평가도 가능하지만, 관련 문헌이나 실제 경험으로 보아 이런 주제를 처음으로 제기하는 단계에서는 이것이 불가피하다고 판단된다. 여기서 언급되었던 문제들의 특성과 유형에 대한 공감대가 확산되어 이를 근거로 후속연구에서는 좀더 체계화된 자료수집이 가능해지기를 기대해본다. 마지막으로 양성평등적 관점은 대학사회의 학문적 적실성을 고양하고 사회가 요구하는 인력양성에도 필요하다는 논거를 통해 여교수의 세력화와 여자교수에 대한 학술담론화가 필요함을 결론으로 제시했다. This paper argues for studies on women professors based on the strategical premise of the Gender Empowerment Indices(GEM) of the Human Development Report, released annually by the UNDP. This paper firstly explores on the possible reasons on the dearth of the literature which deals with gender-discriminating experiences of women professors in their academic, teaching and social experiences. It then proceeds to classify patterns of discriminatory practices and presented cases for each pattern, based on direct and indirect participant observation by the author of the cases of herself and those of colleagues. Critics upholding to a methodological rigor might make a case of subjective hermeneutics out of this study, but the importance of raising the issue for encouraging academic discourse about and empowering women professors are seen to surpass the methodological concern at this incipient stage of its academic endeavor. Still, further studies are expected to enhance inter-subjectivity of the interpretation and explanation provided here with a more women professors willing to study and be studied about lived everyday lives of their profession. This paper closes with the argument on why that universities have to employ gender-sensitive perspectives in not only curriculums and numbers but the definition, organization, and evaluation of routine performances.

빈집을 활용한 지역별 셰어하우스 공급 활성화 방안 - 주거서비스 기반형 주택임대관리업 적용가능성을 중심으로 -

김승희 ( Kim Seunghee ),임혜순 ( Im Hyesoon ),백승훈 ( Paeik Seunghoon ) 한국부동산분석학회 2018 不動産學硏究 Vol.24 No.3

Empty houses have been recognized as being subject to demolition according to regional characteristics due to aging and urban decline. In recent years, however, cases have been increasingly used in response to various spatial needs. In particular, Special Law on empty Houses’ which is enacted in Feb, 2017 has raised social interest in the use of empty houses. The purpose of this study is to find ways to stimulate the supply of sharehouses, which is emerging as an alternative housing type, by using the empty houses that are increasing based on ‘the Population and Housing Census’ of the National Statistical Office. And, this study explores the activation of supply of empty houses suitable for the tenant class by exploiting available resources in the region. In addition, ‘the management business for housing lease’ system implemented in 2013 has been reemphasized due to the increase in the number of rental housing management company. Currently, the management business for housing lease is expected to accelerate market growth in order to protect the rights of tenants and landlords. Therefore, this study will examine the business structure of sharehouses supply based on the characteristics of empty houses by region and the tenant's residential housing service by applying it to the management business for housing lease.