다탐색(多探索) 법을 통한 저자극성 액체 세정제 조성물 개발

김배환 ( Peter Kim ),현기안 ( Ki-an Hyeon ),정지윤 ( Ji-youn Chung ),윤삼숙 ( Sanm-sook Yoon ),강한철 ( Han Chyul Kang ),박선희 ( Sun Hee Park ),고강일 ( Kang Il Ko ),김기호 ( Ki Ho Kim ) 대한화장품학회 2005 대한화장품학회지 Vol.31 No.1

세정제에 널리 상용화되어 있는 알킬에톡시설페이트 계의 음이온 계면활성제는 피부 흡착의 특성 때문에 충분히 헹구어 내지 않으면 피부에 잔존되어 자극의 원인이 되어 염증 반응을 일으키게 된다. 따라서 기존의 음이온 계면활성제를 대체 또는 보완하기 위해 기존의 계면활성제들을 단백질 변성 실험, 세포 독성 그리고 IL-1α 측정을 통해 선별하였다. 본 연구는 저자극성의 음이온 또는 비이온, 양쪽성 이온의 14종의 계면활성제와 민감성 피부를 위해 제조된 13종의 기존 세정제 제품에 대한 세포독성을 실시하여, 가장 독성이 낮은 계면활성제로 sodium laureth sulfate (음이온), sodium cocoyl isethionate (음이온), sodium lauroamphoacetate (양성이온), cocamidopropyl betaine (양성이온), alkyl polyglycoside (비이온)가 선택되었고 2종의 기존 세정제 제품을 비교 제형으로 선택하였다. 5종의 계면활성제를 20종의 formulation으로 제조하여 단백질 변성(<3M SLS (13.2%)), 세포독성 실험 및 폐첩포 실험을 통하여 다시 5종을 선별하였다. 이들 제형을 진피 배양으로 세포독성 및 IL-1α 방춘량을 조사하여 가장 자극이 낮은 계면활성제 제형을 선택하였으며, 첨가된 계면활성제의 자극을 완화하기 위하여 항염과 보습 효과가 우수한 마치현 추출물(3%, 5%)과 fructan (3%, 5%)을 농도별로 첨가한 제형을 제조하여 가장 안전성이 뛰어난 농도를 선택하였다. 최종적으로 선택된 제형 5번을 3차원 세포 배양을 통한 인공피부를 이용하여 가장 자극이 낮게 나타난 기존의 제품들과 세포 독성 및 IL-1α 방출량을 비교 조사하여 저자극성의 액체 세정제를 개발하였다. Alkyl ethoxy sulfate type surfactants, widely used in commercial cleansers, are easily adsorbed to skin to often cause skin irritation and inflammation if not thoroughly rinsed nut. In order to replace or complement existing surfactants, we screened the existing surfactants through protein denaturation method, cell cytotoxicity assay and human IL-1α assay, etc. Fourteen surfactants have been chosen from among too irritant anionic, cationic and/or zwitter-ionic ones and investigated for cell cytotoxicity in human fibroblast cell lines using monolayer culture with the thirteen commercially available cleansers for sensitive skin. From these results, we selected 5 surfactants and 2 commercial cleansers (names not shown), such as sodium laureth sulfate (anionic), sodium cocoyl isethionate (anionic), sodium lauroamphoacetate (zwitter-ionic), and cocamidopropyl betaine (zwitter-ionic), alkyl polyglycoside (non-ionic). 20 formulations were made out of 5 surfactants and five of them were chosen through a protein denaturation method (lower than 3 M sodium dodecyl sulfate solution (13.2%)), cell cytotoxicity and human patch test. These five selected formulations containing preservatives were compared to two selected commercial cleansers by cell cytotoxicity and human IL-1α ELISA assay using dermal equivalent. Finally, we selected the best formulation. To this formulation, fructan (3% or 5%) or/and portulaca extract (3% or 5%) well known for its anti-inflammatory and moisturizing effects were added and investigated for cell cytotoxicity using dermal equivalent. In cytotoxicity assay using dermal equivalent, two formulations containing 5% fructan and 3% or 5% portulaca extract were less toxic than the others. In cytotoxicity assay and human IL-1α ELISA using 3D culture, the selected formulation containing 5% fructan and 5% portulaca extract showed better efficiency than those of the others and 2 commercial cleansers. As a result, we could develop a low irritant and safe liquid cleanser.

백혈병 마우스 모델의 동종골수이식에서 활성화된 자연살해세포들의 보충이 이식편대백혈병효과와 이식편대숙주반응에 미치는 영향

엄현석,한치화,박수정,김소연,정낙균,정대철,진종률,최일봉,양형모,서영훈,송현근,최인표,민우성,김춘추 대한조혈모세포이식학회 2001 대한조혈모세포이식학회지 Vol.6 No.1

배경: 백혈병에서 동종골수이식 (allogeneic bone marrow transplantation)의 성공적 치료 효과를 얻기 위해서는 이식편대숙주반응 (graft-versus-host disease, GVHD) 발생의 극복과 재발의 방지가 중요한 과제이다. 골수를 역류원심성 세포분리 (counterflow centrifugal elutriation, CCE) 방법으로 분리하여 얻은 rotor off (R/O) 세포분획은 T 세포의 수는 적지만 조혈모세포들을 다량 포함하고 있어 동종골수이식에서 주조직적합복합체 (major histocompatibility complex, MHC) 차이를 극복할 수 있고, 이식편의 생착 성공과 GVHD 발생 예방에 효과적이다. 그러나 골수로부터 T 세포를 제거하면 백혈병세포를 공격하는 이식편대백혈병 (graft-versus-leukemia, GVL) 효과가 감소되기 때문에 백혈병 재발의 빈도가 높다. 자연살해세포 (natural killer cell, NK cell)의 보충 첨가는 동종골수이식 후 GVHD 발생을 줄이면서 충분한 GVL 효과를 얻을 수가 있다. 따라서 저자는 분리 후 IL-2로 활성화시킨 NK 세포들을 골수 R/O 세포분획과 함께 백혈병 마우스 모델에 동종이식함으로써 GVHD와 GVL에 미치는 효과를 관찰하였다. 방법: Balb/c (H-2^(d)) 마우스에서 유래된 A20 (murine B-lymphoma/leukemia cell line, H-2^(d)) 백혈병 세포를 이식 2 일 전에 Balb/c 마우스에 주입하고, 치사량의 전신 방사선을 조사한 직후에 Balb/c 또는 C57BL/6 (H-2^(b)) 마우스의 골수 R/O 세포분획을 꼬리정맥을 통하여 주입하였다. 이들은 모두 이식 후 6-8 주 이내에 사망하였다. 동종이식의 대조군 (n=9)에는 1 × 10^(7)의 R/O 세포분획만을 주입하였고, 실험군 (n=9)에는 C57BL/6 마우스의 비장세포들로부터 단클론항체들을 이용한 negative selection방법으로 분리한 후 IL-2로 활성화된 5 × 10^(5)의 NK 세포분획을 1 × 10^(7)의 R/O 세포분획과 함께 주입하였다. GVL 효과의 판정은 이식 후 14 일과 28 일 째 되는 날 마우스에서 골수, 비장, 간 등을 얻어 백혈병 세포들의 침윤을 조직학적으로 관찰하였으며, GVHD의 정도는 육안적 관찰법으로 평가하였다. 결과: R/O 세포분획만을 이식한 대조군의 골수, 비장, 그리고 간 조직에서는 A20 백혈병 세포의 침윤이 각각 89% (8/9), 78% (7/9)와 22% (2/9)에서 관찰되었고, R/O 세포분획과 NK 세포분획을 함께 이식한 실험군에서는 비장과 간을 제외한 골수에서만 89% (8/9)에서 A20 백혈병 세포의 침윤이 관찰되어 두 군 사이에 장기별 분포의 차이를 볼 수 있었다 (P= 0.0001). 한편 GVHD는 두 군 모두에서 경하게 나타나서 유의한 차이는 없었다. 또한 생착 부전으로 사망한 마우스는 없었다. 결론: CCE를 이용하여 T 세포를 제거한 동종골수이식에서 NK 세포의 보충은 GVHD의 악화는 일으키지 않으면서, 백혈병의 진행을 억제하는 GVL 효과를 얻을 수 있었다. Background: Allogeneic bone marrow transplantation (BMT) with T cell-depleted marrow accompanies engraftment failure and relapse of leukemia by a loss of the graft-versus-leukemia (GVL) effect frequently, while it can prevent GVHD. Supplement of NK cells could prevent GVHD and enhance GVL effect in several murine allogeneis BMT models Roter off (R/O) cell fraction obtained by counterflow centriation elutriatio (CCO) contains small number of T cells and many hematopoietic stem cells. The aim of this study was to determine the effect of R/O cell fraction supplemented with IL-2 activated NK cells on GVL and GVHD within the leukemic mouse BMT model. Methods: Inoculation of A20 (H-2d, murine B-lymphoma/leukemia, Balb/c origin) cells into Balb/c mice via the tail vein 2 days prior to lethal total body irradiation (TBI) and infusion of the Balb/c BM or C57BL/6 (H-2b) R/O fraction were performed. It resulted in 100% mortality within 6 to 8 weeks. The irradiated mice in the control group were injected with 1 × 107 R/O cell fraction alone (n=9) and in the experimental group mice were injected with 1 × 107 R/O cell fraction plus 5 × 105 negatively selected IL-2 activated NK cell fractions of the spleens via the tail vein (n=9). On day 14 and 28 after BMT, the bone marrows, spleens, and livers of mice were harvested for histopathologic analysis of the infiltrations of leukemic cells. We then evaluated the GVHD within the mice. Results: A histopathologic study of the recipients receiving R/O fraction alone showed infiltration of leukemic cells, 89% (8/9) in bone marrows, 78% (7/8) in spleens, and 22% (2/9) in livers. The experimental group of mice showed only the infiltration of leukemic cells 89% (8/9) in bone marrows, not in spleens and livers. There were the organ differences of the leukemic cells infiltrations between the two groups (P=0.0001). There were no obvious differences in the GVHD scores between these two groups, and severe GVHD was not observed. There was no engraftment failure among groups. Conclusion: Thus, our findings suggest that R/O cell fraction obtained by CCE and supplemented with NK cells can promote GVL effect without mediating clinically overt GVHD in allogeneic BMT of mouse leukemia.

CAM을 이용한 다지 분기 구조의 설계

조현묵,한일국,기장근 公州大學校工科大學生産技術硏究所 1996 論文集 Vol.5 No.-

In this thesis, Multi-way branch structure is designed to execute multiway branch instruction in the wireless LAN protocol processor. Because this structure substitute CAM(Content Addressable Memory) for target selection unit with label index information of program which branch in multiway according to system variables consist of flags & vectors. The CAM store a set of arbitrary variable conditions. Thus, this structure have feature which assign system value for multiway branch, can programming and reconfigure a set of variable conditions. The Desing of Multiway branch structure used cmn8a library which is 0.8um process of Compass tool, have about 9,500 gates and suitable for basis clock 80MHz of wireless LAN protocol processor.

태권도 수련이 삶의 질과 정신건강에 미치는 영향

이한경,남승현,김석일 龍仁大學校 武道硏究所 1997 武道硏究所誌 Vol.8 No.2

The purpose of this research was to analyze the mental status of a Taekwondo participant and a non-participant. The following were the results. 1) The analysis of Taekwondo participant´s relationship to life and quality were satisfacy at job, leisure, daily life, and steady manage. Consistency in Taekwondo training reduces job stress and physical fatigueness. 2) Taekwondo participant, who trains frequently finds quality of life more satisfying as a hobby and a genuine effect of the art. 3) Taekwondo participant, who trains consistently in one´s qality of life can attain affirnation of positive effects, depending on the individual. 4) In Taekwondo training, there are 9 mental health existence and non-existence. Sometization Obsessive-compulsive, Interpersonal-sensitive, Anxiety, Hastility. Phobic anxiety are all positively effective through Taekwondo training, However, the rest of the following fectors (Depression, paranoid ideation, Dsychoticism) are not effcetive.

스포츠 센터 평생 회원들의 체련장 이용 실태에 관한 연구

이한경,유호길,김미정,김석일,남승현 龍仁大學校 1998 용인대학교 논문집 Vol.15 No.-

The purpose of this study is to analyze privileged mass' use of the sport for all facilities. The subject was members of five in hotel, who answered a questionnaire. The main results of this study are as follows : 1. It was found that comsumers of fitness center were at 40-50 age, and the mean of using period is longer than 3 years. 2. The membership card motivated members to improve, their health and in the older members losed self-health condition. 3. The members were used lower at facilities less. The mean of using frequency is one per four days. 4. It was found that sauna room was the most utilized facilities in fitness center. 5. It was fecund that training room was utilized less, because of lack of time. 6. The participant of training room not showed lower facility use at higher age. 7. The participator of trainning room did showed satisfaction for instructor and facilities. As started above, the turn of recognition which answers the purpose is needed for a privileged mass' use of facilities for sports for all, and a positive motivation of the managements of commercial sports center must be prepared indispensably. Also, a consideration for a resasonable and step by step remedy for all age groups is deeply demanded.

반도체 계측 설비의 실시간 스테이지 모니터링 기술의 개발

한현규(Hyeon-gyu Han),최성일(Sung-il Choi),이상현(Sang-hyeon Lee),이배진(Bae-jin Lee),동승훈(Seung-hoon Tong) 대한전자공학회 2023 대한전자공학회 학술대회 Vol.2023 No.11

In this paper, we developed a system for measuring new parameters of stage accuracy in measurement images using image-processing technology in machine vision facilities. Through control technology, that links the system with Fault Detection Classification (FDC) technology, realtime trends can be managed and preemptively dealt with to prevent loss and quality accidents. In addition, it is intended to be an opportunity to generate and monitor various parameters using measurement images.

간 종물 없이 흉벽에서만 발견된 전이성 간세포암종

현일식 ( Hyeon Il Sig ),조경란 ( Jo Gyeong Lan ),백상현 ( Baeg Sang Hyeon ),김진배 ( Kim Jin Bae ),최호순 ( Choe Ho Sun ),이오영 ( Lee O Yeong ),한동수 ( Han Dong Su ),손주현 ( Son Ju Hyeon ),함준수 ( Ham Jun Su ),이민호 ( Lee Min 대한소화기학회 2003 대한소화기학회 추계학술대회 Vol.2003 No.-

원발성 간세포암종은 간 외로 전이하는 경우가 드문 암이다. 전이가 있는 경우 주로 폐, 복부 임파선, 뼈, 뇌 순으로 전이된다. 그러나, 전이성인 경우라도, 간 종물 없이 전이 병변만 발견된 경우는 보고된 바가 없었다. 이에 간 종물 없이 흉벽에서만 발견된 전이성 간세포암종을 경험하였기에 보고하는 바이다. 51세 남자로, 좌상부 흉벽에 약 12×10㎝ 크기로 단단한 종물이 촉지되고 8개월 전부터 크기가 증가되어 내원하였다. 환자는 10년 전 만성 B형

생체 부분 간이식에서 CT영상을 이용한 간 체적계산법의 Reference Data로서의 유용성

한성일(Seong Il Han),손진현(Jin Hyeon Son),김종옥(Jong Ok Kim),정종성,이제호(Je Ho Lee),김현범(Hyeon Beom Kim) 대한전산화단층기술학회 2009 대한CT영상기술학회지 Vol.11 No.1

It aimed at suggesting the concrete mensuration of lⅣer volume calculation and checking the utility by comparing the difference between the volume data which we have measured at 3D-lab. by reducing the error bound of the measured data and the data which is substantiaⅡy measured at the surgery room. It considered if there is the value of reference data of the volume data which is measured as manual method. The average weight of the graft which had been resected at the substantial surgery room was 680.53±137.35g, and the manual measurement volume average volume was 797.87±165.86㎤. Two data and correlation evaluation appeared to be(γ=0.83535 P〈0.001). The gap between the volume data of manual method and the volume data which was substantiaⅡy measured was 117.93±13.97%, and it shows significant gap in figure. The reason is plausible to be regarded as the reduction of volume(or mass) reduction due to the bleed at the time of operation resection. Meanwhile, what shows the considerably significant gap as 95% CI for the mean(114.72% to 121.14%, P=0.016 in the ratio of the gap) is judged to be valuable as the reference data before the surgery.

Diphenylhydantoin에 의한 중증 재생불량성 빈혈

김한성 ( Kim Han Seong ),한경근 ( Han Gyeong Geun ),김종현 ( Kim Jong Hyeon ),김경환 ( Kim Gyeong Hwan ),조강일 ( Jo Gang Il ),정찬수 ( Jeong Chan Su ),정철호 ( Jeong Cheol Ho ),제영성 ( Je Yeong Seong ),임창현 ( Im Chang Hyeon 대한내과학회 1993 대한내과학회지 Vol.44 No.5

Since 1938, Diphenylhydantoin (DPH) has remained one of the most widely used anticonvulsants for treatment and prevention of seizures. DPH may induce serious damage to the hematopoietic system. Especially, DPH induced severe aplastic anemia is one of the most severe complication and its incidence is extremely rare. We recently expreienced one case of severe aplastic anemia in 52-year-old woman following administration of DPH. Litreature was reviewed briefly.

[PG-0019] Genetic Diversity and Relationship of Rapeseed (Brassica napus L.) Mutant Genotypes Based on Genotyping-by-Sequencing (GBS)

Ba-Wool Yang(Ba-Wool Yang ),Dong-Gun Kim(Dong-Gun Kim ),Sang Hoon Kim(Sang Hoon Kim ),Ye-Jin Lee(Ye-Jin Lee ),Sang-Il Han(Sang-Il Han ),So Hyeon Baek(So Hyeon Baek ),Joon-Woo Ahn(Joon-Woo Ahn ),Chang- 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-