Striatal Dopamine Transporter Availability is Associated with Sleep Disturbance among Patients with Bipolar I Disorder: A Single-photon Emission Computed Tomography Study Using [99mTc] TRODAT-1

Tsung-Hua Lu(Tsung-Hua Lu),Shih-Hsien Lin(Shih-Hsien Lin),Huai-Hsuan Tseng(Huai-Hsuan Tseng),Yen Kuang Yang(Yen Kuang Yang),Nan Tsing Chiu(Nan Tsing Chiu),Po See Chen(Po See Chen) 대한정신약물학회 2022 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.20 No.4

Objective: Bipolar disorder (BD) is characterized by the poor sleep quality. Whether the striatal dopamine transporter (DAT) availability is related to sleep quality among patients with BD is unclear. Methods: Fifty-three euthymic patients with BD (24 BD-I and 29 BD-II) and sixty-eight healthy controls were enrolled. The Chinese Version of the Pittsburgh Sleep Quality Index (PSQI) was used, and the availability of DAT was assessed by single-photon emission computed tomography (SPECT) using [99mTc] TRODAT-1. Results: The sleep disturbance component of the PSQI was significantly associated with the level of DAT availability among patients with BD. Conclusion: The striatal dopaminergic activity that contributes to resilience to adversity was associated with sleep pattern among patients with BD.

Harm Avoidance is Correlated with the Reward System in Adult Patients with Attention Deficit Hyperactivity Disorder: A Functional Magnetic Resonance Imaging Study

Tsung-Hua Lu(Tsung-Hua Lu),Shih-Hsien Lin(Shih-Hsien Lin),Mei Hung Chi(Mei Hung Chi),Ching-Lin Chu(Ching-Lin Chu),Dong-Yu Yang(Dong-Yu Yang),Wei Hung Chang(Wei Hung Chang),Po See Chen(Po See Chen),Yen 대한정신약물학회 2023 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.21 No.1

Objective: Hypoactivity in the reward system among patients with attention deficit hyperactivity disorder (ADHD) is a well-known phenomenon. Whether the activity in the reward pathway is related to harm avoidance, such as in sensitivity to punishment, is unclear. Evidence regarding the potential difference between ADHD patients and controls in terms of this association is scarce. Methods: Event-related functional magnetic resonance imaging was conducted on subjects performing the Iowa gambling test. Fourteen adults with ADHD and 14 controls were enrolled in the study. Results: Harm avoidance was found to be positively correlated with the activities of the bilateral orbitofrontal cortex and right insula in individuals with ADHD. A group difference was also confirmed. Conclusion: Understanding the roles of harm avoidance and brain activation during risk tasks is important.

Impact of a Glyphosate-Tolerant Soybean Line on the Rhizobacteria, Revealed by Illumina MiSeq

( Gui-hua Lu ),( Yin-ling Zhu ),( Ling-ru Kong ),( Jing Cheng ),( Cheng-yi Tang ),( Xiao-mei Hua ),( Fan-fan Meng ),( Yan-jun Pang ),( Rong-wu Yang ),( Jin-liang Qi ),( Yong-hua Yang ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.3

The global commercial cultivation of transgenic crops, including glyphosate-tolerant soybean, has increased widely in recent decades with potential impact on the environment. The bulk of previous studies showed different results on the effects of the release of transgenic plants on the soil microbial community, especially rhizosphere bacteria. In this study, comparative analyses of the bacterial communities in the rhizosphere soils and surrounding soils were performed between the glyphosate-tolerant soybean line NZL06-698 (or simply N698), containing a glyphosate-insensitive EPSPS gene, and its control cultivar Mengdou12 (or simply MD12), by a 16S ribosomal RNA gene (16S rDNA) amplicon sequencing-based Illumina MiSeq platform. No statistically significant difference was found in the overall alpha diversity of the rhizosphere bacterial communities, although the species richness and evenness of the bacteria increased in the rhizosphere of N698 compared with that of MD12. Some influence on phylogenetic diversity of the rhizosphere bacterial communities was found between N698 and MD12 by beta diversity analysis based on weighted UniFrac distance. Furthermore, the relative abundances of part rhizosphere bacterial phyla and genera, which included some nitrogen-fixing bacteria, were significantly different between N698 and MD12. Our present results indicate some impact of the glyphosate-tolerant soybean line N698 on the phylogenetic diversity of rhizosphere bacterial communities together with a significant difference in the relative abundances of part rhizosphere bacteria at different classification levels as compared with its control cultivar MD12, when a comparative analysis of surrounding soils between N698 and MD12 was used as a systematic contrast study.

魏秀仁及其相关人物考证与《花月痕》新解

???(Hua Xiaochen),?薇(LU Wei) 동아인문학회 2020 동아인문학 Vol.50 No.-

The interpretation of Hua Yue Hen has long been confined to the text itself, while the research about the author, the real life of related figures, and the social context is insufficient. Based upon the historical materials about Wei Xiuren’s life experiences and the social context in which the book was written, this paper aims to probe the gist of the novel and the author’s motive from a humanist perspective. Furthermore, a defense of the novel is included from some of its criticisms.

论东亚文化圈三国汉字文化产业的发展现状, 开发方向与完善对策

LU JING HUA,곽다예 동북아시아문화학회 2022 동북아 문화연구 Vol.1 No.72

Chinese Culture Circle is the world with Chinese characteries as media and has the same value system. Chinese culture circle of East Asia refers to the countries and of east Asia such as China, Korea, Japan, Vietnam and so on, This paper mainly focus on and conduct analysis and research on China, South Korea, and Japan, three representative countries in the East Asian Chinese character circle. It is committed to combining the experience advantages of the cultural industries of these three countries to propose a Chinese character cultural industry. The targeted countermeasures will make a contribution to promoting the development of the Chinese character culture industry in practice in the vast number of countries in the East Asian cultural circle. Although China, South Korea, and Japan in the East Asian cultural circle are all belong to the field of Chinese character culture and have many similarities in culture, there are great differences in the way of using Chinese characters. Therefore, for the development of the Chinese character culture industry, countries in the Chinese character culture circle should make efforts in four aspects. In view of the above policies, efforts should be made for the revitalization and development of the Chinese character culture industry according to the actual situation of each country. Humans have language, and language is the most basic information. Since modern times, some Chinese scholars have followed Saussure's erroneous theory. It is hoped that from now on, China and all countries in the East Asian Chinese character culture circle can fundamentally continue to establish and further strengthen the character confidence and even cultural confidence in our East Asian script circle. Lu Jianming believes that global integration makes language an intangible resource that plays an increasingly important role in national economy and security.

miR-424-5p regulates apoptosis and cell proliferation via targeting Bcl2 in nucleus pulposus cells

Lu Hua-tuo,Xu Yong-qing,Wang Hai,Zhang Xu-lin 한국통합생물학회 2020 Animal cells and systems Vol.24 No.3

Background: miRNAs play an important role in the pathogenesis of intervertebral disc degeneration (IDD). The role and the underlying mechanism of miR-424-5p in human nucleus pulposus (NP) are still unknown. We aimed to explore the role of miR-424-5p in IDD. Methods: Real-time PCR was used to detect the expression of miR-424-5p and Bcl2 in IDD tissues and idiopathic scoliosis tissues. Human NP cells were used in our study. MTT and Hoechst apoptosis assays were used to detect the proliferation and apoptosis of NP cells, respectively. Western blotting assays were used to detect the expression levels of Bcl-2, cleaved caspase-3, cleaved caspase-9, caspase-3 and caspase-9 in degenerative NP cells. A luciferase reporter assay was applied to confirm the relationship between miR-424-5p and Bcl2. Results: Our results showed that the expression of miR-424-5p was increased and Bcl2 was decreased in degenerative NP cells. miR-425-5p expression was negatively correlated with Bcl2 expression in IDD tissues. Suppression of miR-424-5p using an inhibitor increased Bcl2 expression at both the mRNA and protein levels, and it promoted cell viability and inhibited apoptosis. Furthermore, the levels of cleaved caspase-3 and cleaved caspase-9 were downregulated in miR-424-5p-silenced NP cells. Interestingly, we found that silencing miR-424- 5p increased p62 expression at both the mRNA and protein levels. Finally, a luciferase reporter assay verified the binding of the miR-424-5p and the 3’UTR of Bcl2. Conclusion: These results suggested that silencing miR-424-5p suppressed NP cell apoptosis by upregulating Bcl2. Therefore, miR-424-5p might be a novel target for IDD therapies.

The Tense and Aspect System of Chinese, English and Korean : Focusing on the present, the past and the future

Lu, Jing-Hua 대한중국학회 2016 중국학 Vol.57 No.-

There are grammatical markers of tense that are obligatory in English and Korean, ho wever, these grammatical temporal forms are absent in Chinese. Although it is uncontrov ersial that Chinese does not morphologically encode tense, there are several other ways in which temporal information can be encoded (Smith&Erbaugh, 2005). Chinese is an aspect system language which marks aspect. On the contrast, English is a tense system languag e which marks tense. In our review of languages considered in this study, the main focus will be on aspect system and tense system which are illustrated by Chinese, English and Korean. The author will illustrate that the tense and aspect system of Chinese is different from that of Korean and English. Our argument in this paper focuses on three categories which can be referred to as the present, past and future. In chapter one, the motive of re search that difference of aspect and tense expression in Chinese, English and Korea was introduced and the course of this research was mentioned. A contrasting study was conducted in chapter two. As for contrasting the characteristics of three languages, the author hope that this paper would help to learn Chinese, English or Korean easily.

Separation of Nattokinase from Bacillus subtilis Fermentation Broth by Expanded Bed Adsorption with Mixed-mode Adsorbent

Lu Miao-Hua,Lin Dong-Qiang,Wu Yuan-Chun,Yun Jun-Xian,Mei Le-He,Yao Shan-Jing The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.2

Mixed-mode hydrophobic/ionic matrices exhibit a salt-tolerant property for adsorbing target protein from high-ionic strength feedstock, which allows the application of undiluted feedstock via an expanded bed process. In the present work, a new type of mixed-mode adsorbent designed for expanded bed adsorption, Fastline $PRO^{\circledR}$, was challenged for the capture of nattokinase from the high ionic fermentation broth of Bacillus subtilis. Two important factors, pH and ion concentration, were investigated with regard to the performance of nattokinase ad-sorption. Under initial fermentation broth conditions (pH 6.6 and conductivity of 10 mS/cm) the adsorption capacity of nattokinase with Fastline PRO was high, with a maximum capacity of 5,350 U/mL adsorbent. The elution behaviors were investigated using packed bed adsorption experiments, which demonstrated that the effective desorption of nattokinase could be achieved by effecting a pH of 9.5. The biomass pulse response experiments were carried out in order to evaluate the biomass/adsorbent interactions between Bacillus subtilis cells and Fastline PRO, and to demonstrate a stable expanded bed in the feedstock containing Bacillus subtilis cells. Finally, an EBA process, utilizing mixed-mode Fastline PRO adsorbent, was optimized to capture nattokinase directly from the fermentation broth. The purification factor reached 12.3, thereby demonstrating the advantages of the mixed-mode EBA in enzyme separation.

Protective effects of 2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone against hydrogen peroxide-induced oxidative stress in hepatic L02 cell

Lu, Yue,Zhang, Yan-Yan,Hu, Ying-Chun,Lu, Yan-Hua 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.9

2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone (DMC) is a chalcone isolated from the buds of Cleistocalyx operculatus (Roxb.) Merr. et Perry, and the hepatoprotective effects of DMC on Kunming mice have been studied in previous study. However, the effects of DMC on hepatocyte toxicity and corresponding mechanism remain unclear. The aim of this study was to evaluate the hepatoprotective mechanism of DMC in human hepatocytes (L02) treated with $H_2O_2$. The results demonstrated that pretreatment with DMC effectively protected $H_2O_2$-induced cell viability loss, cell membrane damage (lactate dehydrogenase, nitric oxide production and caspase-3 accumulation. Besides, DMC pretreatment increased the amount of glutathione, decreased malondialdehyde and the percentage of apoptotic L02 cells compared with only $H_2O_2$ treated group. Taken together, these results indicated that DMC had hepatoprotective effects against $H_2O_2$-induced liver injury by alleviating oxidative stress and apoptosis process in L02 cells, and DMC might be a potential candidate for the intervention of liver diseases.

A Study on Chinese Pidgin English

Lu, Jing-Hua(여정화) 경성대학교 인문과학연구소 2013 인문학논총 Vol.33 No.-

서로 다른 언어를 쓰는 사람들이 원활한 의사소통을 위해서 형성한 혼성어이다. 중국 피진어는 중국어와 영어의 혼성어이며 현재까지도 많은 사람들이 중국 피진어를 사용하고 있다. 최근 몇 년 동안 중국과 외국 언어학자들은 중국 피진어에 큰 관심을 가져왔다. 많은 학자들이 중국 피진어에 대한 자신들의 의견을 개진해 왔다. 본 논문은 중국어와 영어의 언어자료에 근거하여, 사회언어학의 관점에서 보는 중국 피진 영어의 역사적 형성과정과 언어적 특징을 체계적으로 분석하였다. 이 분야를 연구하거나 관심이 있는 분들에게 도움이 되었으면 한다. A Pidgin is a language variety created for the purpose of communicating with some other language groups. Historically, pidgins were used as main media between many different language groups. Chinese Pidgin English, is a Pidgin language between Chinese and English, which is still used by many Chinese. In recent years, domestic and foreign linguists have had a great interest in Chinese Pidgin English. Many scholars have put forward their own opinions on Chinese Pidgin English. Based on the Chinese and English corpora, this paper will make a summary on the historical process and the linguistic features of Chinese Pidgin English from the perspective of sociolinguistic, which is systematic and diverse.