Correlation and Path Analysis of Association among Yield, Micronutrients, and Protein Content in Rice Accessions Grown Under Aerobic Condition from Karnataka, India

Nguyen Phuong Thuy(Nguyen Phuong Thuy ),Nguyen Ngoc Trai(Nguyen Ngoc Trai ),Bui Dang Khoa(Bui Dang Khoa ),Nguyen Hoang Xuan Thao(Nguyen Hoang Xuan Thao ),Vuong Tuan Phong(Vuong Tuan Phong ),Quach Van 한국육종학회 2023 Plant Breeding and Biotechnology Vol.11 No.2

Genetic variability and correlation analysis are fundamental references for the innovative development of breeding programs to improve varieties and desirable traits. In the present study, the correlation and path analysis was conducted to understand the association among yield, micronutrients (iron and zinc), and protein content under aerobic conditions in local rice landraces from various agro ecological regions of Karnataka, India. The grain yield per plant showed a significant positive correlation with plant height, the tiller number, spikelet fertility, flag leaf length, and test weight. The zinc content was negatively correlated with grain yield per plant. The phenotypic path-coefficient analysis revealed that the total number of tillers, grain length, test weight, and harvest index exhibited a positive direct effect on grain yield per plant, while Grain protein content showed a low direct effect on this trait. This study also indicated that harvest index, flag leaf length, spikelet fertility, and test weight could be considered to make for a higher yield.

Four novel mutations in the androgen receptor gene from Vietnamese patients with androgen insensitivity syndrome

Nguyen Thu Hien,Nguyen Duc Quan,Kim Lien Nguyen Thi,Thi Thanh Ngan Nguyen,Nguyen Thi Phuong Mai,Tran Ngoc Dung,Nguyen Huy Hoang 한국유전학회 2023 Genes & Genomics Vol.45 No.4

Background Androgens and androgen receptor (AR) are critical regulators of the masculinization process in male sexual development. The absence of a functioning AR results in the development of the androgen insensitivity syndrome (AIS), a rare disorder of sexual development (DSD) characterized by the external genitalia feminization, gynecomastia, and impaired spermatogenesis. Objective To determine the AR gene mutations associated with male DSD in four unrelated Vietnamese patients. Methods To detect the disease-causing mutations, whole exome sequencing (WES) was performed on four patients diagnosed with AIS. Sanger sequencing was then used for validation of the identified mutations. Finally, 12 web-based tools, three-dimensional protein modeling software, and the guidelines issued by the American College of Medical Genetics and Genomics were used to assess the potential pathogenicity of these mutations. Results Four distinct novel mutations, namely c.1834T > A (p.Cys612Ser), c.2122 C > G (p.Leu708Val), c.2630T > G (p.Phe877Cys), and c.2641 C > A (p.Leu881Met) in the AR gene, were identified in four AIS patients using WES. The in silico analysis results revealed that the Cys612, Leu708, Phe877, and Leu881 sites are important for an appropriate response to androgens of the AR, and mutation at these sites can have adverse effects on the AR functions, androgen–AR interaction, and AR signaling pathway. Conclusions WES and in silico analyses strongly suggested that four novel AR mutations are pathogenic and have led to the development of AIS in the four Vietnamese patients under consideration.

Design of Thermostable Nanogel Systems for Use in the White Tiger Basement Reservoir to Increase the Effectiveness of Crude Oil Production

Nguyen Phuong Tung,Nguyen Thi Phuong Phong,Nguyen Hoang Duy,Nguyen Quoc Hung 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.52 No.5

This research was conducted to experimentally screen the suitability of acrylamido-2- methylpropane sulfonic acid-acrylamide (AMPS-AM) copolymer-based nanogels for using as sealants to shut-off water channels in the high-temperature and high-salinity reservoir conditions of the White Tiger oil feld. Via in situ intercalative free-radical copolymerization, nanogels have been prepared from montmorillonite-alkyl ammonium clay, AMPS and AM. Two primary compositions of nanogel systems were chosen. A mixture of hexamethylenetetramine (HMTA)/phenyl acetate (PhAc) was used as a crosslinking system. The results obtained from screening the gel properties by using the bottle test method, the test under nitrogen pressure and core ooding under imitated reservoir conditions show that the NANOSEAL-1 and the NANOSEAL-2 nanogels can well satisfy the VietsovPetro (VSP) technical requirements for water isolation materials. This research was conducted to experimentally screen the suitability of acrylamido-2- methylpropane sulfonic acid-acrylamide (AMPS-AM) copolymer-based nanogels for using as sealants to shut-off water channels in the high-temperature and high-salinity reservoir conditions of the White Tiger oil feld. Via in situ intercalative free-radical copolymerization, nanogels have been prepared from montmorillonite-alkyl ammonium clay, AMPS and AM. Two primary compositions of nanogel systems were chosen. A mixture of hexamethylenetetramine (HMTA)/phenyl acetate (PhAc) was used as a crosslinking system. The results obtained from screening the gel properties by using the bottle test method, the test under nitrogen pressure and core ooding under imitated reservoir conditions show that the NANOSEAL-1 and the NANOSEAL-2 nanogels can well satisfy the VietsovPetro (VSP) technical requirements for water isolation materials.

Some characters of bacterial cellulases in goats' rumen elucidated by metagenomic DNA analysis and the role of fibronectin 3 module for endoglucanase function

Nguyen, Khanh Hoang Viet,Dao, Trong Khoa,Nguyen, Hong Duong,Nguyen, Khanh Hai,Nguyen, Thi Quy,Nguyen, Thuy Tien,Nguyen, Thi Mai Phuong,Truong, Nam Hai,Do, Thi Huyen Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.5

Objective: Fibronectin 3 (FN3) and immunoglobulin like modules (Ig) are usually collocated beside modular cellulase catalytic domains. However, very few researches have investigated the role of these modules. In a previous study, we have sequenced and analyzed bacterial metagenomic DNA in Vietnamese goats' rumen and found that cellulase-producing bacteria and cellulase families were dominant. In this study, the properties of modular cellulases and the role of a FN3 in unique endoglucanase belonging to glycosyl hydorlase (GH) family 5 were determined. Methods: Based on Pfam analysis, the cellulases sequences containing FN3, Ig modules were extracted from 297 complete open reading frames (ORFs). The alkaline, thermostability, tertiary structure of deduced enzymes were predicted by AcalPred, TBI software, Phyre2 and Swiss models. Then, whole and truncated forms of a selected gene were expressed in Escherichia coli and purified by His-tag affinity column for assessment of FN3 ability to enhance enzyme activity, solubility and conformation. Results: From 297 complete ORFs coding for cellulases, 148 sequences containing FN3, Ig were identified. Mostly FN3 appeared in 90.9% beta-glucosidases belonging to glycosyl hydrolase family 3 (GH3) and situated downstream of catalytic domains. The Ig was found upstream of 100% endoglucanase GH9. Rarely FN3 was seen to be situated downstream of X domain and upstream of catalytic domain endoglucanase GH5. Whole enzyme (called XFN3GH5 based on modular structure) and truncate forms FN3, XFN3, FN3GH5, GH5 were cloned in pET22b (+) and pET22SUMO to be expressed in single and fusion forms with a small ubiquitin-related modifier partner (S). The FN3, SFN3 increased GH5 solubility in FN3GH5, SFN3GH5. The SFN3 partly served for GH5 conformation in SFN3GH5, increased modules interaction and enzyme-soluble substrate affinity to enhance SXFN3GH5, SFN3GH5 activities in mixtures. Both SFN3 and SXFN3 did not anchor enzyme on filter paper but exfoliate and separate cellulose chains on filter paper for enzyme hydrolysis. Conclusion: Based on these findings, the presence of FN3 module in certain cellulases was confirmed and it assisted for enzyme conformation and activity in both soluble and insoluble substrate.

Effect of salicylic acid and yeast extract on curcuminoids biosynthesis gene expression and curcumin accumulation in cells of Curcuma zedoaria

Nguyen Hoang Loc,Truong Thi Phuong Lan,Nguyen Duc Huy,Nguyen Ngoc Luong,Hoang Tan Quang,Trinh Huu Tan,Le Thi Anh Thu,Nguyen Xuan Huy 한국식물생명공학회 2019 JOURNAL OF PLANT BIOTECHNOLOGY Vol.46 No.3

The aim of this study is to evaluate the effect of yeast extract (YE) and salicylic acid (SA) on the expression of curcuminoid-biosynthesis genes (CzDCS and CURS1-3), and accumulation of curcumin in Curcuma zedoaria cell cultures. The results showed that, in cells treated with YE or SA, the expression levels of curcuminoid genes were 1.14- to 3.64-fold higher than the control (untreated cells), in which the YE exhibited a stronger effect in comparison with SA. Curcumin accumulation also tended to be similar to gene expression, curcumin contents in YE- or SA-treated cells were 1.61- to 2.53-fold higher than the control. The SA treatment at the fifth day of culture stimulated the curcumin accumulation and expression in all four genes compared to that at the beginning. While the YE treatments gave different results, the CzCURS1 and CzCURS3 genes were expressed strongly in cells that were treated at the beginning. However, the CzDCS and CzCURS2 genes showed the opposite expression pattern, they were activated strongly in the treatments at day five of the culture. However, the content of curcumin reached its maximum value on the fifth day of culture in all investigations.

The Effectiveness of Financial Sources for Climate Change in Vietnam

Thi Nhung NGUYEN(Thi Nhung NGUYEN ),Minh Hoa NGUYEN(Minh Hoa NGUYEN ),Thi Phuong Anh VU(Thi Phuong Anh VU ),Thi Hoang Anh DO(Thi Hoang Anh DO ) 한국유통과학회 2023 The Journal of Asian Finance, Economics and Busine Vol.10 No.1

This research aims to give information about the current situation of five financial sources for climate change in Vietnam, including (i) the State budget used by ministries; (ii) the State budget used by provinces; (iii) Bilateral funds; (iv) Multilateral funds; and (v) Private funds, and then classify them in line with the effectiveness. The working paper’s secondary data on spending on CC-related activities, collected from reports of six ministries and 29 provinces, show that the State budget has been crucial in subsidizing CC-related activities in Vietnam. Moreover, domestic investment has accounted for a major part of the total expenditure of ministries and provinces for climate change. In addition, by using primary data collected from surveys sent to twelve experts from 5 groups, such as researchers, practical experts, managers of private funding organizations (such as banks and enterprises), managers of international funding organizations and beneficiaries, and then analyzing the data through the AHP method, the study shows that all climate finance sources in Vietnam are still not very effective. However, private sector funds are considered the most effective financial source for responding to climate change.

Cytotoxic and anti-tumor activities of lignans from the seeds of Vietnamese nutmeg Myristica fragrans

Phuong Thien Thuong,Tran Manh Hung,Nguyen Minh Khoi,Hoang Thi My Nhung,Nguyen Thi Chinh,Nguyen Thi Quy,장태수,나민균 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.3

Four lignans, meso-dihydroguaiaretic acid(DHGA), macelignan, fragransin A2 and nectandrin B,were isolated from the seeds of Myristica fragrans (Vietnamesenutmeg) and investigated for their cytotoxicactivity against eight cancer cell lines. Of these, DHGAexhibited potent cytotoxicity against H358 with IC50 valueof 10.1 lM. In addition, DHGA showed antitumor activityin allogeneic tumor-bearing mice model.

Detection of Vibrio cholerae O1 by Using Cerium Oxide Nanowires - Based Immunosensor with Different Antibody Immobilization Methods

Phuong Dinh Tam,Nguyen Luong Hoang,Hoang Lan,Pham Hung Vuong,Ta Thi Nhat Anh,Tran Quang Huy,Nguyen Thanh Thuy 한국물리학회 2016 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.68 No.10

In this work, we evaluated the effects of different antibody immobilization strategies on the response of a CeO2-nanowires (NWs)-based immunosensor for V ibrio cholerae O1 detection. Accordingly, the changes in the electron-transfer resistance (Ret) from before to after cells bind to an antibody-modified electrode prepared by using three different methods of antibody immobilization were determined. The values were 16.2%, 8.3%, and 6.65% for the method that utilized protein A, antibodies activated by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC)/Nhydroxysuccinimide (NHS), and absorption, respectively. Cyclic voltammetry confirmed that the change in the current was highest for the immunosensors prepared using protein A (11%), followed by those prepared with EDC/NHS-activated antibodies (9%), and finally, those prepared through absorption (7.5%). The order of the antibody immobilization strategies in terms of resulting immunosensor detection limit and sensitivity was as follows order: absorption (3.2 × 103 CFU/mL; 45.1 /CFU·mL−1) < EDC/NHS-activated antibody (1.0 × 103 CFU/mL; 50.6 /CFU·mL−1) < protein A (1.0 × 102 CFU/mL; 65.8 /CFU·mL−1). Thus, we confirmed that the protein A - mediated method showed significantly high cell binding efficiencies compared to the random immobilization method.

Implementation of an interval Based expert system for diagnosis of Oriental Traditional Medicine

Nguyen Hoang Phuong,Uong Huong Duong,Yun Sik Kwak,Hune Cho 한국지능정보시스템학회 2001 한국지능정보시스템학회 기타 간행물 Vol.- No.-

Logistics Revolution for e-commerce in Vietnam: A Brief Review

Hoang Phuong Nguyen,Minh Tuan Nguyen,Minh Tuan Pham 국제이네비해양경제학회 2019 International Journal of e-Navigation and Maritime Vol.13 No.1

For the whole process of circulation, distribution, application of the logistics system is a higher development step of transportation technology. Multimodal transport has linked all modes of transport together to serve the supply and consumption needs of the manufacturer. Currently, when e-commerce (EC) is on the rise, quality logistics and delivery services are the essential stages to achieve success in transactions. e-commerce today is no longer a fashion trend; it is an essential part of the retail industry. EC is changing the game of Logistics. The e-commerce world has been introducing many risks and complex issues that logistics and supply chain experts have never faced. E-commerce is the main impact on terminal logistics (last mile) and warehousing. However, it is also quite interesting to see how e-commerce is impacting airports and seaports. E-commerce is growing actively in Vietnam at a rate of about 25% per year, which is an excellent opportunity for business logistics services to get more orders. Moreover, the Vietnamese logistics market is small but has a high growth rate (20% - 25% / year). In recent times, this market is expected to change the face and grow faster due to the development of electronic retail in Vietnam and e-Logistics logistics trends in the world. However, to meet the requirements, to grasp the opportunities, logistics enterprises need to link to get a closed and synchronized service chain. So logistics companies need to increase information, respond faster, enhance technology to meet flexible processes, establish networks, and business links to meet changing needs. The article shows that the inadequacies and lack of cohesion between logistics service providers and e-commerce are limiting competitiveness, losing opportunities of enterprises, and the economy in the context of healthy industrial revolution 4.0. The assessment of the correlation and connectivity of logistics and e-commerce enterprises in Vietnam in the paper will contribute significantly to the development of Vietnam's economy in the period 2020-2025.