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Establishment of hybridization breeding of Ganoderma lucidum by protoplast monokaryogenesis method
Wu Jiaojiao,Fu Lizhong,Wu Xueqian,Xu Xiuhong,Li Haibo,Wu Qingqi,Wei Hailong,Cheng Junwen 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4
Four Ganoderma lucidum strains, Chizhi 05, Jingda, Huizhou and Xinzhou, were screened out as hybrid parent in order to establish G. lucidum cross breeding system that based on protoplast monokaryogenesis method. Monokaryotic strains of each parental strains were obtained and mating type of each monokaryotic strains were determined. One to three monokaryotic strains that have different mating types were mated, and hybrids were identified by clamp connection observation and antagonist response. The results showed that the number of monokaryon came from Chizhi 05, Jingda, Huizhou and Xinzhou was 9, 14, 40 and 38, respectively. Only one mating type was obtained from Jingda, and two mating types were obtained from the other three strains, Chizhi 05, Huizhou and Xinzhou, respectively. Chi-square test showed that the ratio of two mating types of the three strains was 1:1. Fourteen monokaryotic strains of different mating types from 4 parental strains were select as a cross- breeding materia, and 17 hybrids were obtained, which were identified by clamp connection observation and antagonist response. This study proclaimed that the practicality of the hybridization breeding of G. lucidum by protoplast monokaryogenesis method.
Haibo Jiang,Haozhen Fang,Jinpeng Wu,Zhuangcheng Fang,Shu Fang,Gongfa Chen 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.45 No.6
Steel–precast ultra-high-performance concrete (UHPC) composite beams with demountable high-strength frictiongrip bolt (HSFGB) shear connectors can be used for accelerated bridge construction (ABC) and achieve excellent structural performance, which is expected to be dismantled and recycled at the end of the service life. However, no investigation focuses on the demountability and reusability of such composite beams, as well as the installation difficulties during construction. To address this issue, this study conducted twelve push-out tests to investigate the effects of assembly condition, bolt grade, bolt– hole clearance, infilling grout and pretension on the crack pattern, failure mode, load–slip/uplift relationship, and the structural performance in terms of ultimate shear strength, friction resistance, shear stiffness and slip capacity. The experimental results demonstrated that the presented composite beams exhibited favorable demountability and reusability, in which no significant reduction in strength (less than 3%) and stiffness (less than 5%), but a slight improvement in ductility was observed for the reassembled specimens. Employing oversized preformed holes could ease the fabrication and installation process, yet led to a considerable degradation in both strength and stiffness. With filling the oversized holes with grout, an effective enhancement of the strength and stiffness can be achieved, while causing a difficulty in the demounting of shear connectors. On the basis of the experimental results, more accurate formulations, which considered the effect of bolt–hole clearance, were proposed to predict the shear strength as well as the load–slip relationship of HSFGBs in steel–precast UHPC composite beams.
Measurement of the radon and thoron exhalation rates from the water surface of Yixin lake
Jiulin Wu,Shuaibin Liu,Tao Hu,Fen Lin,Ruomei Xie,Shuai Yuan,Haibo Yi,Yixiang Mo,Jiale Sun,Linquan Cheng,Huiying Li,Zhipeng Liu,Zhongkai Fan,Yanliang Tan Korean Nuclear Society 2024 Nuclear Engineering and Technology Vol.56 No.4
The importance of determining the radon exhalation rate from water surface is emphasized by the increased use of radon and its daughter products as tracers in large-scale circulation studies of the atmosphere. There were many methods to measure radon exhalation from water surface. With the development of radon exhalation rate measurement methods and instruments on the surface of the soil, the rock and building materials, so the radon exhalation rate from water surface can be more accurately measured by applying these improved methods and instruments. In this paper, a cuboid accumulation chamber surrounded by foam boards and a RAD7 were used to measure the radon exhalation rate on the water surface at three different positions by Yixin lake. Each measurement was performed 2 h. The radon exhalation rate from the water surface was about 6 × 10<sup>-3</sup> Bq m<sup>-2</sup>s<sup>-1</sup>. The thoron exhalation rate from the water surface also can be estimated, it is about 0.16 Bq m<sup>-2</sup>s<sup>-1</sup>. These results hint that the radon transmission from the lake bottom soil to water and then into the atmosphere.
Effect of Brewing Conditions on Taste Components of Keemun Black Tea
Eunhye Kim,Haibo Pan,Xiao Mao,Guangwei Wang,Yuanyuan WU,Youying Tu 한국차학회 2015 한국차학회지 Vol.- No.S
Tasting and quality components of tea infusion are the important standard of tea quality. The chemicals of tea infusion influent the sanitarian function and quality, and also are key factors of products for consumers. The effects of brewing time and temperature on extractability of Keemun black tea were studied. The results showed that the contents of tea polyphenols, theaflavins, thearubigins and caffeine were obviously affected by temperature at 80℃, however, no significant effect on the other components. The results also showed that the TP/AA ratio content, suggested that 80℃ boiling water could brew best taste in Keemun black tea.
New receipt-free voting scheme using double-trapdoor commitment
Chen, Xiaofeng,Wu, Qianhong,Zhang, Fangguo,Tian, Haibo,Wei, Baodian,Lee, Byoungcheon,Lee, Hyunrok,Kim, Kwangjo Elsevier 2011 Information sciences Vol.181 No.8
<P><B>Abstract</B></P><P>It is considered to be the most suitable solution for large scale elections to design an electronic voting scheme using blind signatures and anonymous channels. Based on this framework, Okamoto first proposed a receipt-free voting scheme <ce:cross-ref refid='b0150'>[30]</ce:cross-ref> for large scale elections. However, in the following paper, Okamoto <ce:cross-ref refid='b0155'>[31]</ce:cross-ref> proved that the scheme <ce:cross-ref refid='b0150'>[30]</ce:cross-ref> was not receipt-free and presented two improved schemes. One scheme requires the help of the parameter registration committee and the other needs a stronger physical assumption of the voting booth. In this paper, we utilize the double-trapdoor commitment to propose a new receipt-free voting scheme based on blind signatures for large scale elections. Neither the parameter registration committee nor the voting booth is required in our scheme. We also present a more efficient zero-knowledge proof for secret permutation. Therefore, our scheme is much more efficient than Okamoto’s schemes <ce:cross-refs refid='b0150 b0155'>[30,31]</ce:cross-refs> with the weaker physical assumptions. Moreover, we prove that our scheme can achieve the desired security properties.</P>
Xian Wang,Shaolei Ma,Haibo Wu,Xiaofeng Shen,Shiqin Xu,Xirong Guo,Maria L Bolick,Shizheng Wu,Fuzhou Wang 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-
Our previous works disclosed the contributing role of macrophage migration inhibitory factor (MIF) and dopaminergic inhibition by lysine dimethyltransferase G9a/Glp complex in peripheral nerve injury-induced hypersensitivity. We herein propose that the proinflammatory cytokine MIF participates in the regulation of neuropathic hypersensitivity by interacting with and suppressing the descending dopaminergic system. The lumbar spinal cord (L-SC) and ventral tegmental area (VTA) are two major locations with significant upregulation of MIF after chronic constriction injury (CCI) of the sciatic nerve, and they display time-dependent changes, along with a behavioral trajectory. Correspondingly, dopamine (DA) content shows the reverse characteristic change to MIF with a time-dependent curve in post-surgical behavior. The levels of both MIF and DA are reversed by the MIF tautomerase inhibitor ISO-1, and a negative relationship exists between MIF and DA. The reversed role of ISO-1 also affects tyrosine hydroxylase expression. Furthermore, CCI induces Th promoter CpG site methylation in the L-SC and VTA areas, and this effect could be abated by ISO-1 administration. G9a/SUV39H1 and H3K9me2/H3K9me3 enrichment within the Th promoter region following CCI in the L-SC and VTA was also decreased by ISO-1. In cultured dopaminergic neurons, rMIF enhanced the recruitment of G9a and SUV39H1, followed by an increase in H3K9me2/H3K9me3. These molecular changes correspondingly exhibited alterations in Th promoter CpG site methylation and pain behaviors. In summary, MIF functions as a braking factor in curbing dopaminergic descending inhibition in peripheral nerve injury-induced hypersensitivity by mediating Th gene methylation through G9a/SUV39H1-associated H3K9 methylation.
Analysis of genetic diversity of Ganoderma strains based on rDNA ITS sequencing
Fu Lizhong,Zhang Hongyu,Wu Xueqian,Li Haibo,Wei Hailong,Wu Qingqi,Wang Li’an 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4
The genetic diversity of 16 Ganoderma strains was investigated by rDNA-ITS sequencing. Alignment analysis showed that whole length of internal transcribed spacer(ITS1+ITS2) was 500bp and with 139 variation sites (accounting for 23.3%, ITS1 was 66 and ITS2 was 73), 337 conserved sites (accounting for 72.2%), 59 informative sites (accounting for 9.88%), 86 conversion sites (G-A, C-T), 13 transversion site(C-G, T-A). The ratio of transition and transversion in ITS1 was higher than that in ITS2, and the variable sites of ITS2 were more than those of ITS1. The genetic distance among 16 Ganoderma strains is from 0 to 0.121. The genetic distance between G. lipsiense and F-1 was 0, and the genetic distance between Heizhi 02 and Huizhou, Jingda, G. capense was 0.121, 0.117 and 0.120, respectively. The 16 Ganoderma strains were classed into 4 groups. The biggest group is comprised of 12 strains, including Xinzhou, Huizhou, Jingda, 902, F-1, Xianzhi, Meiluo, Taishan, G. applanatum, 05, G. luteomarginatum. The G. atrum and G. sinense were clustered into one group. The G. capense and Zhongzhi was independent group, respectively. These results showed that there were some genetic difference among groups, and there was lower genetic diversity among strains in same groups.