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Cordyceps militaris alleviates non-alcoholic fatty liver disease in ob/ob mice
Ha-Neul Choi,Yang-Hee Jang,Min-Joo Kim,Min Jeong Seo,Byoung Won Kang,Yong Kee Jeong3,,Jung-In Kim 대한지역사회영양학회 2014 Nutrition Research and Practice Vol.8 No.2
BACKGROUND/OBJECTIVES: Non-alcoholic fatty liver disease (NAFLD) is becoming an important public health problem as metabolic syndrome and type 2 diabetes have become epidemic. In this study we investigated the protective effect of Cordyceps militaris (C. militaris) against NAFLD in an obese mouse model. MATERIALS/METHODS: Four-week-old male ob/ob mice were fed an AIN-93G diet or a diet containing 1% C. militaris water extract for 10 weeks after 1 week of adaptation. Serum glucose, insulin, free fatty acid (FFA), alanine transaminase (ALT), and proinflammatory cytokines were measured. Hepatic levels of lipids, glutathione (GSH), and lipid peroxide were determined. RESULTS: Consumption of C. militaris significantly decreased serum glucose, as well as homeostasis model assessment for insulin resistance (HOMA-IR), in ob/ob mice. In addition to lowering serum FFA levels, C. militaris also significantly decreased hepatic total lipids and triglyceride contents. Serum ALT activities and tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels were reduced by C. militaris. Consumption of C. militaris increased hepatic GSH and reduced lipid peroxide levels. CONCLUSIONS: These results indicate that C. militaris can exert protective effects against development of NAFLD, partly by reducing inflammatory cytokines and improving hepatic antioxidant status in ob/ob mice.
Ha-Neul Jeong,Jin Ju Park,Seon Mi Yoo,Chi-Do Wee 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
The aim of this study is to utilize dietary management with precise galactose contents in foods for galactosemia patients. In order to establish an analytical method for improved detection sensitivity upon major free sugar in foods, we compared and estimated LOD, LOQ, and linearity of calibration curve of the use of a liquid chromatography with evaporative light-scattering detector, and the use of a gas chromatography with flame ionization detector. By the method of HPLC/ELSD, the estimated reference standard of galactose through LOD, LOQ, and the correlation coefficient were 80, 240 ppm and 1.000 respectively. However, the developed method with GC/FID after the modified trimethylsilyl-oxime derivatives to its reference standard mixture of 6 major free sugars including galactose, glucose, fructose, sucrose, lactose and maltose, all sugars were separated clearly. Thus, the results of the R2 values were all over 0.999, especially the values of galactose upon LOD and LOQ were estimated to be 0.136 and 0.412 ppm. Therefore, these results indicated that quantitative contents of galactose in foods were able to be analyzed precisely in units of 1ppm by TMSO derivatives and GC/FID method.
Development of Selective and Specific O-glycans Method
Ha Neul Jeong,Myung Jin Oh,Serenus Hua,Hyun Joo An 한국당과학회 2013 한국당과학회 학술대회 Vol.2013 No.1
There are two types of glycosylation, N-linked and O-linked, respectively. N-linked glycan (or N-glycan) is attached to the asparagines (Asn), which is usually released by N-glycosylation specific enzyme known as peptide-N-glycosidase F (PNGase F). O-linked glycan (or O-glycan) is attached to the either serine (Ser) or threonine (Thr), however, there is no enzyme for the universal release of O-glycans from glycoproteins. In general, O-glycans are chemically released by β-elimination, which often release N-glycans that are abundant in human fluids. Thus, O-glycan release still remains analytical challenge in the field of glycomics. Here, we have developed the method to release O-glycans selectively and specifically. Mucin are large glycoproteins that carry dense clusters of O-linked glycans and IgG are glycoproteins that attacted only N-glycans. N and O mixed glycans were prepared in the lab to exist both N-glycans and O-glycans together in a sample. We approached three methods to compare and determine the best method to release selective and specific O-glycans release. 1) Method A : O-glycoproteins were eluted in acetonitrile (ACN) with 0.1% trifluoroacetic acid (TFA) from the cartridge that N-linked glycans were already removed. Glycoproteins were further treated with sodium borohydride (NaBH4) to release O-glycans and then enriched by porous graphitized carbon (PGC). We found that O-glycans were eluted in 20% ACN/H2O. 2) Method B : We separated N-glycans released by PNGase F and glycoproteins in mixed samples using molecular weight cut off (MWCO) 10,000 Da spin-column with centrifugation because glycoproteins molecular weight is higher than N-glycans. Glycoproteins were further treated with NaBH4 to release O-glycans and then enriched by PGC. We found that O-glycans were eluted in 20% ACN/H2O. 3) Method C : Glycoproteins in mixed samples after PNGase F treatment are precipitated by cold 100% ethanol. Glycoproteins were further treated with NaBH4 to release O-glycans and then enriched by PGC. We found that O-glycans were eluted in 20% ACN/H2O. Purified O-glycans were analyzed using Matrix-assisted Laser Desorption/Ionization time-of-flight mass spectrometry (MALDI-TOF MS). Indeed, we found O-glycans such as [Hex]1[HexNAc]2, [Hex]2[HexNAc]4 and [Hex]2[HexNAc]3[Fuc]1. This suggests that O-glycans can be selectively and specifically released under the absence of high abundant N-glycans using our approach. In the future, we will analyze enriched O-linked glycans quantitatively by nano-LC/chip Q-TOF MS to compare three methods. This analytical platform can be widely used for O-glycan release and enrichment.
Jeong, Ha-Neul,Ahn, Sang-Il,Na, Minkyun,Yoo, Jihwan,Kim, Woohyun,Jung, In-Ho,Kang, Soobin,Kim, Seung Min,Shin, Ha Young,Chang, Jong Hee,Kim, Eui Hyun The Korean Neurosurgical Society 2021 Journal of Korean neurosurgical society Vol.64 No.2
Objective : Electrooculography (EOG) records eyeball movements as changes in the potential difference between the negatively charged retina and the positively charged cornea. We aimed to investigate whether reliable EOG waveforms can be evoked by electrical stimulation of the oculomotor and abducens nerves during skull base surgery. Methods : We retrospectively reviewed the records of 18 patients who had undergone a skull base tumor surgery using EOG (11 craniotomies and seven endonasal endoscopic surgeries). Stimulation was performed at 5 Hz with a stimulus duration of 200 μs and an intensity of 0.1-5 mA using a concentric bipolar probe. Recording electrodes were placed on the upper (active) and lower (reference) eyelids, and on the outer corners of both eyes; the active electrode was placed on the contralateral side. Results : Reproducibly triggered EOG waveforms were observed in all cases. Electrical stimulation of cranial nerves (CNs) III and VI elicited positive waveforms and negative waveforms, respectively, in the horizontal recording. The median latencies were 3.1 and 0.5 ms for craniotomies and endonasal endoscopic surgeries, respectively (p=0.007). Additionally, the median amplitudes were 33.7 and 46.4 μV for craniotomies and endonasal endoscopic surgeries, respectively (p=0.40). Conclusion : This study showed reliably triggered EOG waveforms with stimulation of CNs III and VI during skull base surgery. The latency was different according to the point of stimulation and thus predictable. As EOG is noninvasive and relatively easy to perform, it can be used to identify the ocular motor nerves during surgeries as an alternative of electromyography.
제주도 숨은물벵뒤 습지 서식 Alphaproteobacteria 및 Gammaproteobacteria 강에 속하는 신변이주의 특성
김하늘 ( Ha Neul Kim ),강지영 ( Ji Young Kang ),최재희 ( Jae Hee Choi ),최정욱 ( Jeong Uk Choe ),이상훈 ( Sang Hoon Lee ),김태의 ( Tae Ui Kim ),이하나 ( Ha Na Yi ),장광엽 ( Kwang Yeop Jahng ),조장천 ( Jang Cheon Cho ),이현환 ( Hyu 한국환경생물학회 2011 환경생물 : 환경생물학회지 Vol.29 No.3
In this study, samples were collected from the Sumummulbangdui wetland at the Halla Mountain in Jeju Island in order to isolate novel bacterial strain. Bacterial strains belonging to the class Alphaproteobacteria, and Gammaproteobacteria were isolated after spreading samples onto solid agar media. The 16S rRNA gene sequences of the strains assigned to the two classes were compared to those of type strains of the species. The strains that showed less than 98.7% 16S rRNA gene sequence similarity to the validly published species were considered to be novel species candidates. A total of 19 strains were regarded as novel strains which can be regarded as novel species candidates. In the Alphaproteobacteria, 6 novel strains were affiliated with the genera Novosphingobium, and Rhizobium. A total of 13 novel strains belong to Gammaproteobacteria that assigned to the family Moraxellaceae, Pseudomonadaceae, and Enterobacteriaceae were identified. Cultural, physiological, chemotaxonomic characteristics and fatty acids compositions have been determined for the novel species candidates, and the characteristics are described in this study.
Renoprotective and antioxidant effects of Saururus chinensis Baill in rats fed a high-fructose diet
Ha-Neul Choi,Yong-Hyun Park,Ji-Hye Kim,Min-Jung Kang,Soo-Mi Jeong,Hyeon Hoe Kim,Jung-In Kim 한국영양학회 2011 Nutrition Research and Practice Vol.5 No.4
This study investigated the preventive effect of Saururus chinensis Baill against renal damage induced by a high-fructose diet in rats. The rats (n = 30) were fed either a cornstarch-based (65%), high-fructose (65%), or high-fructose (64.5%) diet with 0.5% S. chinensis Baill extract for 10 weeks. Twenty-four hour urine collections were obtained and the animals were sacrificed after an overnight fast. Serum urea and creatinine and urine albumin were measured using colorimetric methods, and creatinine clearance was determined. In addition, thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), and the activity of superoxide dismutase (SOD) in the kidney were determined. Kidney samples were also examined histologically. The fructose-fed rats showed renal dysfunction, indicated by decreased creatinine clearance, increased albumin in the urine, and increased urea and creatinine in the serum. These renal function parameters were comparable to control levels in rats that consumed S. chinensis Baill. Fructose consumption increased renal TBARS and reduced GSH and SOD activity, whereas these levels were near-normal in the rats consuming S. chinensis Baill. The kidneys of fructose-fed rats showed glomerular basement membrane thickening, mesangial matrix expansion, and tubule dilation. These pathological changes were not seen in the rats that consumed S. chinensis Baill. Therefore, S. chinensis Baill effectively alleviated fructose-induced renal damage in these rats, at least partially due to antioxidant activity.