소수성 촉매를 이용한 수소동위원소 교환 반응 측정을 위한 순환 반응기

최희주,이한수,안도희,강희석,김광락 한국공업화학회 2002 응용화학 Vol.6 No.2

The catalytic rate constants of hydrophobic catalysts developed for the removal of tritium mainly generated in the pressurized heavy water reactors should be measured. A recycle reactor was designed and built for characterzing the long-term behavior of the catalyst performance at the various temperatures and gas velocities.The catalytic rate constants were measured at the apparent gas velocity of 0.5 m/s and the temperature of 60℃. The initial catalytic constant was 8.44×10^-4mol/g.sec. This value is much greater than that considered in the design of Wolsong Tritium Removal Facility.

인슐린 비의존형 당뇨병 환자에서 혈장 Endothelin-1농도의 변화

신양수,조희충,김원식,국기용,김용화,정종훈,문철웅,배학연,양성훈 朝鮮大學校 附設 醫學硏究所 1992 The Medical Journal of Chosun University Vol.17 No.2

Endothelin(ET) is a 21-residue peptide originally isolated from the cultured porcine endothelial cells. There are at least three genes for endothelin:endothelin-1(ET-1), endothelin-2(ET-2), and endothelin-3(ET-3). Endothelins are present in various human biological fluids including plasma, urine, breast milk, and saliva and have been found elevated plasma ET concentrations in patients with diabetes mellitus, Patients undergoing maintenance hemodialysis due to chronic renal failure, patients with acute myocardial infarction, and patients with subarachnoid hemorrhage. Endothelial cell damage is suspected to occur in diabetic patients and may be one important cause of angiopathy, a major complication in diabetes mellitus. The elevation of ET in diabetic patients may be a marker of, and further exacerbate, their vascular disease. We measured the levels of ET-1 in plasma of 50 patients with non-insulin dependent diabetes mellitus(NIDDM) and 25 normal subjects by radioimmunoassay. The plasma ET-1 concentration (mean±S.D.) in NIDDM was 6.461 A2.510 f㏖/ ㎖, and was significantly higher than in normal subjects (4.567±1.155f㏖/㎖) (P<0.05). The plasma ET-1 concentration (mean±S.D.) in diabetic retinopathy group( 7.15±2.454 f㏖/ml) was significantly elevated than those in otherwise uncomplicated groups (5.348±2.390 f㏖/㎖)(P<0.01). The correlation between any clinical parameters and plasma ET-1 levels in NIDDM was not significant, In conclusion, this study suggest that the elevated levels of ET-1 in diabetic patients may be play a important role in the pathogenesis of diabetic complications.

New Zealand White 토끼의 생식세포 및 체세포 분열에 의한 염색체 분석

신선희,김희수,최영현,이원호 부산대학교 유전공학연구소 2001 분자생물학 연구보 Vol.17 No.-

토끼(New Zealand White rabbit)의 감수분열 및 유사분령상을 통해 그 염색체적 특징을 조사하였다. 감수분열 염색체의 표본 작성은 공기건조법을 다소 변형하여 사용하였고, 유사분열 중기상의 핵형분석에는 G-분염법을 이용하여 다음과 같은 결과를 얻었다. 1. 제1감수분열의 접합기와 태사기의 염색소립과 sex vesicles 및 동원체가 뚜렷하게 구별되었다. 태사기의 중기에서부터 후기 과정이 진행되는 동안에 2가염색체에서 측면으로 돌출한 머리카락 모양의 돌기들이 관찰되어졌고, 이 돌기들은 염색체가 lampbrush 염색체 구조의 모양이 되도록 해주었다. 이동기의 염색체들은 키아즈마의 수와 위치에 따라 분류가 가능하였다. 제1 감수분열 전기의 후반부 과정과 중기Ⅰ 시기에는 2가염색체로 된 21개의 상염색체와 1개의 말단결합으로 연결된 X-Y 염색체를 관찰할 수 있었다. 2. New Zealand White rabbit의 생식세포에서 관찰된 2가 염색체의 형태는 1CH, 1TAl 및 2TA 2가염색체 유형이 대부분을 이루었다. New Zealand White rabbit에서의 평균 키아즈마 빈도는 약 30.2로 나타났으며, 키아즈마 빈도가 복사기, 이동기 및 중기Ⅰ로 분열이 진행됨에 따라 다소 감소하는 경향이 있었다. 3. New Zealand White rabbit의 핵형분석에 의하면 염색체 수는 44개(2n=44)로서, 8쌍의 중부염색체, 9쌍의 차중부염색체, 4쌍의 단부염색체로 된 21쌍의 상염색체와 중부염색체인 1개의 X 염색체 및 단부염색체인 1개의 Y 염색체로 구성되어 있음을 알 수 있었다. Chromosomal characteristics of New Zealand White rabbit was studied at meiosis and mitosis. The meiotic chromosomal preparations were made with the modified air-drying method and karyotype analysis was performed with the G-banding technique, using isolated mitotic metaphase chromosomes of the New Zealand White rabbit. Chromosomes, sex vesicles and centromeres could be could be classified in the zygotene and the pachytene of the meiosis Ⅰ. The hair-like processes projecting laterally from the axes of bivalent chromosomes at the mid-late pachytene were basis of the numbers and the locations of chiasma in the diakinesis. Twenty-one autosomal bivalents and a single unequal terminally associated X-Y bivalent were observed during the late prophase and the metaphase of the meiosis Ⅰ. Most of the bivalent types observed in the New Zealand White rabbit spermatocytes were 1CH, 1TAl, and 2TA bivalents. The mean chiasma frequency (CE) of the male New Zealand White rabbit was 30.2, and it was found that the CF value tended to decrease through the diakinesis and the metaphase Ⅰ. The karyotype of the New Zealand White rabbit was a male chromosome number of 44(2n=44), comprising 8 pairs of metacentric, 9 pairs of submetacentric, 4 pairs of acrocentric autosomes, metacentric X chromosome and acrocentric Y chromosome.

Inhibitory effects of natural plants of Jeju Island on elastase and MMP-1 expression.

Kim, Young Heui,Kim, Ki Soo,Han, Chang Sung,Yang, Hong Chul,Park, Sun Hee,Ko, Kang Ii,Lee, Soo Hee,Kim, Ki Ho,Lee, Nam Ho,Kim, Jung Mi,Son, Kyung-Hun Society of Cosmetic Chemists 2007 Journal of cosmetic science Vol.58 No.1

<P>In order to search for new active cosmetic ingredients of natural origin, we screened about 60 plants collected from Jeju Island, which is located in the southernmost part of the Republic of Korea. We investigated their free radical scavenging activity, elastase inhibition activity, and reduction of MMP-1 mRNA expression for the development of anti-aging ingredients as raw materials for use in cosmetics. In the free radical scavenging capacity assay, 12 extracts, including Typha orientalis (seed) and Torreya nucifera (leaf), showed significant free radical scavenging activity (up to SC(50)<30 microg/ml). Among these extracts, Nymphaea tetragona (rhizome) extract showed the highest free radical scavenging activity (SC(50)=4.7 microg/ml). In the anti-elastase inhibition assay, seven extracts, including Typha orientalis (seed) and Persicaria hydropiper (whole plant), showed high inhibitory activity (>50% at 100 mug/ml). Among these extracts, Persicaria hydropiper (whole plant) extract showed the highest elastase inhibition activity (IC(50) = 46.7 mug/ml). In the MMP-1 expression assay using RT-PCR, Typha orientalis (seed), Pyrrosia hastata (root), and Capsicum annum (whole plant) showed slightly lower inhibition activity than EGCG, which was used as a control. Furthermore, four extracts, including Persicaria hydropiper (whole plant), Filipendula glaberrima (root), Nymphaea tetragona (root), and Camellia japonica (leaf), completely inhibited the expression of MMP-1 in human fibroblast cells. The results showed that four of the 60 plant extracts may hold potential for use as natural active ingredients for anti-aging cosmetics.</P>

The Food Safety of Superfine Saengshik Processed by Top-down Technique in Mice

Kim, Dong-Heui,Song, Soon-Bong,Qi, Xu-Feng,Kang, Wie-Soo,Jeong, Yeon-Ho,Teng, Yung-Chien,Lee, Seon-Goo,Kim, Soo-Ki,Lee, Kyu-Jae The Korean Society of Toxicogenomics and Toxicopro 2009 Molecular & cellular toxicology Vol.5 No.1

Saengshik is an uncooked and powdered functional food composed of various edible plants, and has been consumed widely due to its health benefits and convenient uptake. Recently, superfine ground saengshik, which contains a certain extent of nanoscale particles, has been commercialized to enhance efficacy, but its safety has not been determined. This study was conducted to evaluate the food safety of superfine saengshik (SS) through general toxicity examination after oral uptake in mice compared to conventional fine saengshik (FS). The SS particle size distribution was 0.479-26.303 f.1m in diameter, with about 68.92% of particles with a diameter < $0.955{\mu}m$. From our safety evaluation, the number of white blood cells (WBCs) and biochemical values in the serum fell into the normal range, and the weight of organs showed no significant difference between FS and SS groups. Histological observation of the liver, small intestine and large intestine did not show any abnormal or pathological findings under light microscopy. Our results suggest that oral intake of SS is not harmful to mice in terms of general toxicity.

Molecular Characterization of the HERV-W Env Gene in Humans and Primates: Expression, FISH, Phylogeny, and Evolution

Kim, Heui-Soo,Kim, Dae-Soo,Huh, Jae-Won,Ahn, Kung,Yi, Joo-Mi,Lee, Ja-Rang,Hirai, Hirohisa Korean Society for Molecular Biology 2008 Molecules and cells Vol.26 No.1

We characterized the human endogenous retrovirus (HERV-W) family in humans and primates. In silico expression data indicated that 22 complete HERV-W families from human chromosomes 1-3, 5-8, 10-12, 15, 19, and X are randomly expressed in various tissues. Quantitative real-time RT-PCR analysis of the HERV-W env gene derived from human chromosome 7q21.2 indicated predominant expression in the human placenta. Several copies of repeat sequences (SINE, LINE, LTR, simple repeat) were detected within the complete or processed pseudo HERV-W of the human, chimpanzee, and rhesus monkey. Compared to other regions (5'LTR, Gag, Gag-Pol, Env, 3'LTR), the repeat family has been mainly integrated into the region spanning the 5'LTRs of Gag (1398 bp) and Pol (3242 bp). FISH detected the HERV-W probe (fosWE1) derived from a gorilla fosmid library in the metaphase chromosomes of all primates (five hominoids, three Old World monkeys, two New World monkeys, and one prosimian), but not in Tupaia. This finding was supported by molecular clock and phylogeny data using the divergence values of the complete HERV-W LTR elements. The data suggested that the HERV-W family was integrated into the primate genome approximately 63 million years (Myr) ago, and evolved independently during the course of primate radiation.

Genomic Features of Retroelements and Implications for Human Disease

Kim, Heui-Soo Korea Genome Organization 2005 Genomics & informatics Vol.3 No.4

Most of the endogenous retroviral genes integrated into the primate genome after the split of New World monkeys in the Oligocene era, approximately 33 million years ago. Because they can change the structure of adjacent genes and move between and within chromosomes they may play important roles in evolutionas well as in many kinds of disease and the creation of genetic polymorphism. Comparative analysis of HERVs (human endogenous retroviruses) and their LTR (long terminal repeat) elements in the primate genomes will help us to understand the possible impact of HERV elements in the evolution and phylogeny of primates. For example, HERV-K LTR and SINE-R elements have been identified that have been subject to recent change in the course of primate evolution. They are specific elements to the human genome and could be related to biological function. The HERV-M element is related to the superfamily of HERV-K and is integrated into the periphilin gene as the truncated form, 5'LTR-gag-pol-3'LTR. PCR and RT-PCR approaches indicated that the insertion of various retrotransposable elements in a common ancestor genome may make different transcript variants in different primate species. Examination of the HERV-W elementrevealed that env fragments were detected on human chromosomes 1, 3-7, 12, 14, 17, 20, and X, whilst the pol fragments were detected on human chromosomes 2-8, 10-15, 20, 21, X, and Y. Bioinformatic blast search showed that almost full-length of the HERV-W family was identified on human chromosomes 1-8, 11-15, 17, 18, 21, and X. Expression analysis of HERV-W genes (gag, pol, and env) in human tissues by RT-PCR indicated that gag and pol were expressed in specific tissues, whilst env was constituitively expressed in all tissues examined. DNA sequence based phylogenetic analysis indicated that the gag, pol and env genes have evolved independently during primate evolution. It will thus be of considerable interest to expand the current HERV gene information of various primates and disease tissues.

Identification and phylogenetic analysis of the human endogenous retrovirus HERV-W pol in cDNA library of human fetal brain

Kim, Heui-Soo,Jeon, Seung-Heui,Yi, Joo-Mi,Kim, Tae-Hyung,Lee, Won-Ho Korean Society of Life Science 2003 생명과학회지 Vol.13 No.3

인간 내생 레트로바이러스 HERV-W는 다발성 경화증 환자로부터 탐지된 MSRV와 연루되어 있다. 인간 태아의 뇌로부터 유래된 cDNA library를 이용하여 PCR법으로 2개의 HERV-W 패밀리(HWP-FB10과 HWP-FB12)를 동정하고 분석하였다. 그들은 HERV-W (accession no. AF009668)와 89%의 염기서열의 유사성을 보였다. Pol 유전자를 아미노산의 서열로 분석해 본 결과 점돌연변이 또는 삽입/결실로 말미암아 frameshift 및 종결코돈을 나타내었다. 유전자정보의 데이터베이스를 이용하여 HERV-W 패밀리간의 분자계통분류도를 작성해 본 결과 HWP-FB10은 인간의 염색체 7q21-22로부터 유래된 AC000064와 매우 가깝게 관련되어 있음을 시사하였다. 이들의 새로운 HERV-W pol 패밀리가 이웃하는 어떤 유전자와 상호 연결되어 있으며, 어떠한 기능을 수행하는지에 대한 전망에 대해 토의하였다. A human endogenous retroviral family (HERV-W) has recently been described that is related to multiple sclerosis-associated retrovirus (MSRV) sequences that have been identified in particles recovered from monocyte cultures from patients with multiple sclerosis. Two pol fragments (HWP-FB10 and HWP-FBl2) of HERV-W family were identified and analysed by the PCR approach with cDNA library of human fetal brain. They showed 89 percent nucleotide sequence similarity with that of the HERV-W (accession no. AF009668). Deletion/insertion or point mutation in the coding region of the pol fragments from human fetal brain resulted in amino acid frameshift that induced a mutated protein. Phylogenetic analysis of the HERV-W family from GenBank database indicates that the HWP-FB10 is very closely related to the AC000064 derived from human chromosome 7q21-q22. Further studies on the genetic relationship with neighbouring genes and functional role of these new HERV-W pol sequences are indicated.

Comparison of the toxicities of two antihypertensive drugs; fimasartan and amlodipine

Soo-Heui Paik,Yong-Ha Chi,Joo-Han Lee,Ji-Han Kim,Hak-Soo Kim,Si-Whan Song 한국실험동물학회 2010 한국실험동물학회 학술발표대회 논문집 Vol.2010 No.8

Identification and phylogeny of L1PA4 elements belonging to the LINE-1 family in primates

Heui-Jeong Park,Joo-Mi Yi,Tae-Hyung Kim,Won-Ho Lee,Heui-Soo Kim 한국생명과학회 2003 한국생명과학회 심포지움 Vol.39 No.-