Development of an antibody-based diagnostic method for the identification of Bemisia tabaci biotype B

Baek, J.H.,Lee, H.J.,Kim, Y.H.,Lim, K.J.,Lee, S.H.,Kim, B.J. Academic Press 2016 Pesticide biochemistry and physiology Vol.131 No.-

<P>The whitefly Bemisia tabaci is a very destructive pest. B. tabaci is composed of various morphologically undistinguishable biotypes, among which biotypes B and Q, in particular, draw attention because of their wide distribution in Korea and differential potentials for insecticide resistance development. To develop a biotypespecific protein marker that can readily distinguishes biotypes B from other biotypes in the field, we established an ELISA protocol based on carboxylesterase 2 (COE2), which is more abundantly expressed in biotypes B compared with Q. Recombinant COE2 was expressed, purified and used for antibody construction. Polydonal antibodies specific to B. tabaci COE2 [anti-COE2 pAb and deglycosylated anti-COE2 pAb (DG anti-COE2 pAb)] revealed a 3-9-fold higher reactivity to biotype B COE2 than biotype Q COE2 by Western blot and ELISA analyses. DG anti-COE2 pAb exhibited low non-specific activity, demonstrating its compatibility in diagnosing biotypes. Western blot and ELISA analyses determined that one of the 11 field populations examined was biotype B and the others were biotype Q suggesting the saturation of biotype Q in Korea. DG anti-COE2 pAb discriminates B. tabaci biotypes B and Q with high specificity and accuracy and could be useful for the development of a B. tabaci biotype diagnosis kit for on-site field applications. (C) 2016 Elsevier Inc. All rights reserved.</P>

Magnetic properties of Mn12O12(O2CC4H3S)16(H2O)4 single-molecule magnet

B. J. Kim,S. Yoon,B. J. Suh,J. Kim,J. M. Lim,S. H. Phark,Y. Do,Z. G. Khim 한국물리학회 2004 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.45 No.3

We report a study of the magnetic properties of a Mn12O12(O2CC4H3S)16(H2O)4 single-molecule magnet. Polycrystalline powder samples were prepared by using a reaction of 3-thiophenecarboxylic acid with a slurry of Mn12O12(O2CCH3)16(H2O)4 (noted as Mn12-Ac) in methylene chloride. Magnetization measurements were performed down to 2 K with a magnetic eld of up to 6 T. The magnetization curves showed a superparamagnetic behavior above the blocking temperature TB and became hysteric below TB due to the magnetic anisotropy of the molecule. The spin and the magnetic anisotropy constant of the molecule were determined by analyzing the magnetization data, and the results agree well with those for Mn12-Ac. We also report the chemical deposition of a Mn12O12(O2CC4H3S)16(H2O)4 lm and an observation of its surface morphology by using scanning tunneling microscopy.

A novel canine influenza H3N2 virus isolated from cats in an animal shelter

Jeoung, H.Y.,Lim, S.I.,Shin, B.H.,Lim, J.A.,Song, J.Y.,Song, D.S.,Kang, B.K.,Moon, H.J.,An, D.J. Elsevier Scientific Pub. Co 2013 Veterinary microbiology Vol.165 No.3

The interspecies transmission of avian-origin H3N2 canine influenza virus (CIV) to dogs was first reported in 2007. The present study characterized a novel CIV H3N2 isolated from cats in an animal shelter. A comparative analysis of the deduced amino acid sequences of the A/Canine/Korea/CY009/2010(H3N2) (CY009) and A/Feline/Korea/FY028/2010 (H3N2) (FY028) strains isolated from dogs and cats, respectively, in the animal shelter identified point mutations in 18 amino acid positions within eight viral genes. Interestingly, CY009 and FY028 replicated well in specific pathogen-free embryonated chicken eggs and in mice, respectively. Mice infected with the FY028 strain exhibited significant over expression of IL-10, TNF-α, and IFN-γ (p<0.001) at 3 days postinfection. Thus, an emergency monitoring system should be developed to identify influenza mutations that occur during interspecies transmission in companion animals and for continuous public health surveillance.

Pharmacokinetics of prothionamide in patients with multidrug-resistant tuberculosis.

Lee, H W,Kim, D W,Park, J H,Kim, S-D,Lim, M-S,Phapale, P B,Kim, E-H,Park, S K,Yoon, Y-R The Union 2009 The international journal of tuberculosis and lung Vol.13 No.9

<P>SETTING: National Masan Tuberculosis Hospital, Masan, South Korea. OBJECTIVE: To evaluate the pharmacokinetics of prothionamide (PTH) in South Korean patients with multidrug-resistant tuberculosis (MDR-TB) and to investigate whether differences in body mass index (BMI) could explain observed differences in PTH disposition. DESIGN: Seventeen patients participated in the study; all had MDR-TB and had received combination anti-tuberculosis treatment, including PTH, cycloserine, ofloxacin, para-aminosalicylic acid and streptomycin or kanamycin, for at least 2 weeks. The patients were divided into two groups based on BMI: Group A (18.5 < or = BMI<23), and Group B (BMI<18.5). Serum samples were collected over 24 h, and the plasma PTH concentration was determined by a validated high-performance liquid chromatography assay. RESULTS: After steady-state administration of PTH, the mean area under the plasma concentration-time curve from time 0 to 12 h (AUC(0-12h)) was 11.0 +/- 3.7 microg h/ml. The mean T(max) and t(1/2) were respectively 3.6 h and 2.7 h. No significant difference in PTH disposition was observed between groups A and B, except for ke and t(1/2). CONCLUSION: In the pharmacokinetic parameter estimates for PTH in MDR-TB patients during routine treatment, the pharmacokinetics of PTH did not appear to correlate with extent of emaciation in MDR-TB patients.</P>

Influence of spherical particles and interfacial stress distribution on viscous flow behavior of Ti@?Cu@?Ni@?Zr@?Sn bulk metallic glass composites

Hong, S.H.,Kim, J.T.,Mun, S.C.,Kim, Y.S.,Park, H.J.,Na, Y.S.,Lim, K.R.,Park, J.M.,Kim, K.B. Elsevier Applied Science 2017 INTERMETALLICS Vol.91 No.-

Bulk metallic glass composites containing micro-scale B2 particles are subject to investigation with regards to the influence of B2 particles and interfacial stress and strain distribution on the viscous flow behavior at a super-cooled liquid state. An increased volume fraction of B2 particles leads to an increase of minimum viscosity and influences viscous flow behavior before crystallization. In high temperature deformation, the bulk metallic glass shows homogeneous deformation feature. However, the heterogeneous deformation feature is found in thermoplastically deformed bulk metallic glass composite. The strong stain accumulation and sluggish viscous flow occur around B2 particles, which are caused by the heterogeneous stress distribution linked to stress concentration and shear stress impediment around B2 particles. The sluggish viscous flow of super-cooled liquids around B2 particles during high temperature deformation induces an increase of viscosity and strongly affects the viscous flow behavior of Ti-based bulk metallic glass composites containing micro-scale spherical B2 particles.

과산화수소가 함유된 저산도 질산용액에서 DEHPA 추출제에 의한 Np 의 추출거동

이일희,양한범,김광욱,임재관,유재형 ( E . H . Lee,H . B . Yang,K . K . Kim,J . K . Lim,J . H . Yoo ) 한국공업화학회 1996 공업화학 Vol.7 No.4

본 연구는 Np의 환원제로 H₂O₂가 함유된 IM 이하의 저산도 질산용액으로부터 DEHPA(di-(2-ethyhexyl)phosphoric acid) 추출제에 의한 Np의 추출 및 역추출 조건 설정과 추출속도 향상에 주안점을 두어, 회분식으로 실험을 수행하였다. 저산도 질산용액에서 Np의 산화상태는 주로 Np(V)로 존재하고 있음을 확인하였으며, NP의 추출율은 H₂0₂농도 및 DEHPA의 농도 증가에 따라 증가하고, 질산농도 증가에 따라 급격히 감소하였다. 제3의 산화/환원제가 첨가되지 않는 경우 추출율은 약 70% 정도로 다소 낮지만, DEHPA에 의해 추출이 가능함을 보았다. 또한 추출속도는 H₂O₂농도의 0.516 승에 비례하며, 질산농도의 0.483 승에 반비례하고 있는 다음과 같은 식을 얻었다. d[Np(V)]/dt=-1.391×10^(-2)[H₂O₂]^(0.516)[HNO₃]^(-0.483) [Np(V)] 그리고 과산화수소의 첨가 유무에 관계없이, 유기상으로 추출된 Np은 옥살산(oxalic acid)에 의해 효과적으로 역추출되었으며, 0.5M 옥살산으로 약 92% 이상을 역추출하였다. Extraction behaviour of Np with DEHPA(di-(2-ethyhexyl) phosphoric acid) from the low nitric acid solution(below 1M HNO₃) containing H₂O₂as a reducing agent was studied at a batch system in order to establish the conditions of extraction and stripping and to enhance the extraction rate. As results, it was confirmed that the Np was mainly the pentavalent oxidation state in the low nitric acid solution. The extraction yield of Np was increased with increasing the concentration of DEHPA and H₂O₂and decreased more rapidly with the increase of HNO₃ concentration. It was also found that the Np could be extracted into DEHPA even without the addition any redox agents, although the extraction yield is rather low as about 70%. The extraction rate was proportional to the 0.516 power of H₂O₂concentration and inversely proportional to 0.483 power of HNO, concentration as follows. d[Np(V)]/dt=-1.391×10^(-2)[H₂O₂]^(0.516)[HNO₃]^(-0.483) [Np(V)] Regardless of the H₂O₂, the Np extracted in the organic phase was effectively stripped to the aqueous phase with H₂C₂O₄. The Np could be stripped more than 92% with 0.5M H₂C₂O₄.

200 GeV/핵자 유황이온과 핵건판핵의 충돌에 의해 생성된 헬륨 파쇄핵의 극한파쇄 연구

김동철,송진섭,윤천실,정성헌,박인곤,김종오,김철수,김태연,이승희,조재희,천병구,김재률,김준원,김태익,박명렬,장한일,임인택 慶尙大學校 기초과학연구소 1992 基礎科學硏究所報 Vol.8 No.-

고에너지 중이온 원자핵과 핵건판의 충돌에서, 200GeV/핵자 유황이온에 의해 생성된 파쇄 헬륨핵(Z=2)의 실험실계의 방출각 분포는 표적핵에 무관한 회귀공식. dN=exp[a+k exp(η-y_b)]d[exp(η-y_b)]로 잘 표현된다. 여기에서 의사신속도 η=-ln[tan(θ/2)]이고, y_b는 실험실계의 입사입자(^32S)의 신속도이다. 이 공식에 의한 적합에서 k=-0.057±0.008로 얻어진다. 즉, 핵건판과 고에너지 중이온의 충돌에서 파쇄 헬륨핵의 exp(η-y_b)의 분포는 "극한파쇄" 현상을 잘 설명하고 있다. The angular distribution of emission angle θ of helium (Z=2) produced in the collisions of incident particles of 200 GeV/nucleon ^32S in nuclear emulsion is well expressed by dN=exp[a+k exp(η-y_b)]d[exp(η-y_b)] where the pseudorapidity is η=-ln[tan(θ/2)], the laboratory system primary rapidity is y_b, and k=-0.057+0.008. The shape of this frequency of occurrence distributions in terms of exp(η-y_b) attests to the validity of the concept of "limiting fragmentation" for helium projectile fragments produced in the projectile fragmentation regions of heavy ion collisions in nuclear emulsion.

14.6 A GeV ^28Si 중이온이 원자핵건판내에서 발생시킨 핵반응에서 생성된 2차입자의 발생각 분포

김종오,김태연,남신우,신택수,우종관,이세병,임계엽,장세덕,조재희,천병구,임인택,김기영 慶尙大學校 기초과학연구소 1990 基礎科學硏究所報 Vol.6 No.-

14.6A GeV^28Si 중이온이 원자핵 건판내에서 발생시킨 N_h=1인 핵반응에서 생성된 47개의 파쇄 α 입자와 537개의 단일하전 2차입자의 발생각들을 측정하여 변수 exp(γ-η_b)의 포괄적 분포를 회귀함수 dN=exp[a+χ{exp(γ-η_b)d{exp(γ-η_b)}로 적합시켰다. 여기서 의사신속도 γ=arctanh(cosθ)=-ln tan(θ/2)이고, 입사 중이온의 신속도 η_b=3.445이다. 그 적합결과 파쇄 α입자의 경우 χ=-0.052±0.011이고, 파쇄 p입자의 경우 χ=-0.141±0.015이었다. For LS emission angles of 47 α fragments and 537 single-charged shower particles, produced by the N_h (the number of heavyprongs)=1 interactions of 14.6 A GeV^28Si nuclei in the nuclear emulsion, the distribution of the parameter exp(γ-η_b) is well expressed by dN=exp[a+χ{exp(γ-η_b)d{exp(γ-η_b)}with χ=-0.052±0.011 for αfragments and χ=-0.141±0.015 for p 'fragments', where the pseudorapidity of secondaries γ=arctanh(cosθ)=-ln tan(θ/2) and the rapidity of incident heavy ions, η_b=3.445.

Mechanical and electrical properties of carbon nanotube/Cu nanocomposites by molecular-level mixing and controlled oxidation process.

Lim, Byung K,Mo, Chan B,Nam, Dong H,Hong, Soon H American Scientific Publishers 2010 Journal of Nanoscience and Nanotechnology Vol.10 No.1

<P>A molecular-level mixing and controlled oxidation process is proposed as a novel fabrication technique for the production of CNT/Cu nanocomposite powders. The fabricated CNT/Cu2O nanocomposite powders showed microstructures with homogeneous dispersion of implanted CNTs in a Cu2O matrix. The CNT/Cu2O nanocomposite powders were reduced to CNT/Cu nanocomposite powders with H2 gas and then the as-prepared CNT/Cu nanocomposite powders were spark plasma sintered to fabricate CNT/Cu nanocomposites. The mechanical properties of the Cu and the CNT/Cu nanocomposites were characterized by tensile testing before and after hot compression. Before hot compression, the CNT/Cu nanocomposites were brittle, but after hot compression both yield strength and elongation were increased, while the yield strength of the Cu was decreased after hot compression. Hot compression enhanced the ductility and strength of the CNT/Cu nanocomposites due to alignment of Cu grains and CNTs. Electrical conductivity was also enhanced due to a reduced scattering of electrons because of the alignment of the CNTs and Cu grains as well as the annealing effects of the Cu matrix.</P>

H₂ evolution under visible light irradiation on La and Cr co-doped NaTaO₃ prepared by spray pyrolysis from polymeric precursor

Kang, H.W.,Lim, S.N.,Park, S.B.,Park, A.H.A. Pergamon Press ; Elsevier Science Ltd 2013 INTERNATIONAL JOURNAL OF HYDROGEN ENERGY - Vol.38 No.15

Photocatalysts of Na<SUB>1-x</SUB>La<SUB>x</SUB>Ta<SUB>1-x</SUB>Cr<SUB>x</SUB>O<SUB>3</SUB> and NaTa<SUB>1-x</SUB>Cr<SUB>x</SUB>O<SUB>3</SUB> were prepared by spray pyrolysis from aqueous and polymeric precursor solution. Apart from the contribution of La<SUP>3+</SUP> ions co-doped into NaTa<SUB>1-x</SUB>Cr<SUB>x</SUB>O<SUB>3</SUB> on the BET surface area and the surface morphology by preventing crystal growth, this co-doping contributed to the increased Cr<SUP>3+</SUP> concentration by partially tuning the electron configuration from A<SUP>+</SUP>B<SUP>5+</SUP>O<SUB>3</SUB> to (A<SUP>+</SUP>A'<SUP>3+</SUP>)<SUP>2+</SUP>(B<SUP>5+</SUP>B'<SUP>3+</SUP>)<SUP>4+</SUP>O<SUB>3</SUB> in the lattice of the photocatalyst. Na<SUB>1-x</SUB>La<SUB>x</SUB>Ta<SUB>1-x</SUB>Cr<SUB>x</SUB>O<SUB>3</SUB> prepared from polymeric precursor solution reduced the induction period to 33% and enhanced the hydrogen evolution rate 5.6-fold to 1467.5 μmol g<SUP>-1</SUP> h<SUP>-1</SUP> compared with the equivalent values of NaTa<SUB>1-x</SUB>Cr<SUB>x</SUB>O<SUB>3</SUB> prepared from aqueous precursor. The optimum amounts of dopant and additives comprising the polymeric precursor to maximize the hydrogen evolution rate were x = 0.003 and 300 mol%, respectively.