Antioxidant, Antimutagenic, and In vivo Buccal Mucosa Cancer Preventive Effects of Fructus Malvae

Gui-Jie Li,Qiang Wang,Yu Qian,Xin Zhao 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.4

Fructus Malvae is functional food known forantioxidant, anti-mutagenic, and in vivo anticancer effects. Fructus Malvae extracts demonstrated high antioxidantactivities in DPPH and hydroxyl radical scavenging activityassays. In an Ames mutagenicity test, Fructus Malvaeexhibited antimutagenicty in association with MNNG (Nmethyl-N'-nitro-N-nitrosoguanidine) in Salmonella TyphimuriumTA100 cells. U14 squamous carcinoma cells were injectedinto the buccal mucosa of KM (Kunming) mice. Thewound at the injection site was smeared with a FructusMalvae solution, which was also administered to mice bygavage. Tumor volumes were reduced and tissue sectionanalysis of buccal mucosa cancer and cervical lymph nodetissues showed anti-cancer effects in Fructus Malvaetreated groups. Fructus Malvae possesses good antioxidantand antimutagenic activities and exerts a preventive effectagainst buccal mucosa cancer in vivo.

Preventive Effect of Polysaccharide of Larimichthys crocea Swim Bladder on Reserpine Induced Gastric Ulcer in ICR Mice

Li, Gui-Jie,Sun, Peng,Wang, Rui,Zhou, Ya-Lin,Qian, Yu,Zhao, Xin The Korean Society of Pharmacology 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.2

This project's aim was to determine the reserpine-induced gastric ulcer preventive effect of polysaccharide of Larimichthys crocea swim bladder (PLCSB) in ICR mice. The anti-gastric ulcer effects of polysaccharide of Larimichthys crocea swim bladder was evaluated in mice model using morphological test, serum levels assay, cytokine levels assay, tissue contents analysis, reverse transcription-polymerase chain reaction (RT-PCR) analysis and western bolt assay. High concentration (50 mg/kg dose) of PLCSB reduced IFN-${\gamma}$ as compared to low concentration (25 mg/kg dose) and control mice. SS and VIP serum levels of PLCSB treated mice were higher than those of control mice, and MOT and SP serum levels were lower than control mice. Gastric ulcer inhibitory index of PLCSB treatment groups mice were much lower than control mice, and the high concentration treated mice were similar to the ranitidine treated mice. The SOD and GSH-Px activities of PLCSB treated mice were higher than control mice, close to normal mice and ranitidine treated mice. PLCSB treated mice also showed the similar contents of NO and MDA to normal group. By RT-PCR and western blot assay, PLCSB significantly induced inflammation in tissues of mice by downregulating NF-${\kappa}B$, iNOS, and COX-2, and upregulating $I{\kappa}B-{\alpha}$. These results suggest that PLCSB showed a good gastric ulcer preventive effect as the gastric ulcer drug of ranitidine. Polysaccharide of Larimichthys crocea swim bladder may be used as a drug material from marine products.

Preventive effect of Polysaccharide of Larimichthys Crocea Swimming Bladder on Activated Carbon- Induced Constipation in Mice

Gui-Jie Li,Yu Qian,Peng Sun,Xia Feng,Kai Zhu,Xin Zhao 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.2

Effects of polysaccharide of Larimichthys croceaswimming bladder (PLCSB) on activated carbon-induced constipationin ICR mice were investigated. ICR mice were subjected to oraladministration with lactic acid bacteria for 9 days. Body weight,diet and drinking intake, defecation status, gastrointestinal transit,and defecation time, as well as motilin (MTL), gastrin (Gas),endothelin (ET), somatostatin (SS), acetylcholinesterase (AChE),substance P (SP), and vasoactive intestinal peptide (VIP) levels inserum were used to evaluate the preventive effects of PLCSB onconstipation. Bisacodyl, a laxative drug, was used as a positivecontrol. The time to the first black stool defecation for normal,control, bisacodyl-treated (100 mg/kg), 50 and 100 mg/kg PLCSBtreatedmice were 88, 202, 126, 155, and 135 min, respectively. Following the consumption of oral administration of 50 and 100mg/kg PLCSB or bisacodyl (100 mg/kg), the gastrointestinaltransit was reduced to 62.6, 78.3, and 90.2%, respectively. Theserum levels of MTL, Gas, ET, AChE, SP, and VIP weresignificantly increased, and the serum levels of SS were reducedin the mice treated with PLCSB compared with those in theuntreated control mice (p <0.05). These results demonstrate thatPLCSB has preventive effects on mouse constipation and highconcentration of that demonstrated the better functional activity.

Preventive effect of Polysaccharide of Larimichthys Crocea Swimming Bladder on Activated Carbon-Induced Constipation in Mice

Li, Gui Jie,Qian, Yu,Sun, Peng,Feng, Xia,Zhu, Kai,Zhao, Xin The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.2

Effects of polysaccharide of Larimichthys crocea swimming bladder (PLCSB) on activated carbon-induced constipation in ICR mice were investigated. ICR mice were subjected to oral administration with lactic acid bacteria for 9 days. Body weight, diet and drinking intake, defecation status, gastrointestinal transit, and defecation time, as well as motilin (MTL), gastrin (Gas), endothelin (ET), somatostatin (SS), acetylcholinesterase (AChE), substance P (SP), and vasoactive intestinal peptide (VIP) levels in serum were used to evaluate the preventive effects of PLCSB on constipation. Bisacodyl, a laxative drug, was used as a positive control. The time to the first black stool defecation for normal, control, bisacodyl-treated (100 mg/kg), 50 and 100 mg/kg PLCSB-treated mice were 88, 202, 126, 155, and 135 min, respectively. Following the consumption of oral administration of 50 and 100 mg/kg PLCSB or bisacodyl (100 mg/kg), the gastrointestinal transit was reduced to 62.6, 78.3, and 90.2%, respectively. The serum levels of MTL, Gas, ET, AChE, SP, and VIP were significantly increased, and the serum levels of SS were reduced in the mice treated with PLCSB compared with those in the untreated control mice (p<0.05). These results demonstrate that PLCSB has preventive effects on mouse constipation and high concentration of that demonstrated the better functional activity.

Preventive Effect of Polysaccharide of Larimichthys crocea Swim Bladder on Reserpine Induced Gastric Ulcer in ICR Mice

Gui-Jie Li,Peng Sun,Rui Wang,Yalin Zhou,Yu Qian,Xin Zhao 대한약리학회 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.2

This project’s aim was to determine the reserpine-induced gastric ulcer preventive effect of polysaccharideof Larimichthys crocea swim bladder (PLCSB) in ICR mice. The anti-gastric ulcer effectsof polysaccharide of Larimichthys crocea swim bladder was evaluated in mice model using morphologicaltest, serum levels assay, cytokine levels assay, tissue contents analysis, reverse transcription-polymerase chain reaction (RT-PCR) analysis and western bolt assay. High concentration (50mg/kg dose) of PLCSB reduced IFN-γ as compared to low concentration (25 mg/kg dose) and controlmice. SS and VIP serum levels of PLCSB treated mice were higher than those of control mice, andMOT and SP serum levels were lower than control mice. Gastric ulcer inhibitory index of PLCSBtreatment groups mice were much lower than control mice, and the high concentration treated micewere similar to the ranitidine treated mice. The SOD and GSH-Px activities of PLCSB treated micewere higher than control mice, close to normal mice and ranitidine treated mice. PLCSB treated micealso showed the similar contents of NO and MDA to normal group. By RT-PCR and western blot assay,PLCSB significantly induced inflammation in tissues of mice by downregulating NF-κ B, iNOS, andCOX-2, and upregulating IκB-α . These results suggest that PLCSB showed a good gastric ulcerpreventive effect as the gastric ulcer drug of ranitidine. Polysaccharide of Larimichthys crocea swimbladder may be used as a drug material from marine products.

Prognostic Factors Influencing Clinical Outcomes of Malignant Glioblastoma Multiforme: Clinical, Immunophenotypic, and Fluorescence in Situ Hybridization Findings for 1p19q in 816 Chinese Cases

Qin, Jun-Jie,Liu, Zhao-Xia,Wang, Jun-Mei,Du, Jiang,Xu, Li,Zeng, Chun,Han, Wu,Li, Zhi-Dong,Xie, Jian,Li, Gui-Lin Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.3

Malignant glioblastoma multiforme (GBM) is the most malignant brain tumor and despite recent advances in diagnostics and treatment prognosis remains poor. In this retrospective study, we assessed the clinical and radiological parameters, as well as fluorescence in situ hybridization (FISH) of 1p19q deletion, in a series of cases. A total of 816 patients with GBM who received surgery and radiation between January 2010 and May 2014 were included in this study. Kaplan-Meier survival analysis and Cox regression analysis were used to find the factors independently influencing patient progression free survival (PFS) and overall survival (OS). Age at diagnosis, preoperative Karnofsky Performance Scale (KPS) score, KPS score change at 2 weeks after operation, neurological deficit symptoms, tumor resection extent, maximal tumor diameter, involvement of eloquent cortex or deep structure, involvement of brain lobe, Ki-67 and MMP9 expression level and adjuvant chemotherapy were statistically significant factors (p<0.05) for both PFS and OS in the univariate analysis. Cox proportional hazards modeling revealed that age ${\leq}50$ years, preoperative KPS score ${\geq}80$, KPS score change after operation ${\geq}0$, involvement of single frontal lobe, deep structure involvement, low Ki-67 and MMP9 expression and adjuvant chemotherapy were independent favorable factors (p<0.05) for patient clinical outcomes.

Antibacterial characteristics of glycinin basic polypeptide against Staphylococcus aureus

Jie Yang,Gui-Jin Sun,Ying-Qiu Li,Kai-Yu Cui,Hai Zhen Mo 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.5

This paper aims to study the antibacterial action of glycinin basic polypeptide (GBP) on Staphylococcus aureus (S. aureus). Herein, the minimum inhibitory concentration (MIC) of GBP against S. aureus was 0.2 mg/mL. Atomic force microscopy (AFM) imaging showed that GBP seriously damaged the morphology of the S. aureus cells. GBP (0.8 mg/mL) enhanced the relative release of β- galactosidase to 25.48% when compared to the control. The activity of the respiratory-chain dehydrogenase of S. aureus decreased with increasing GBP concentration. GBP could cause a leakage of intracellular substances. Additionally, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that S. aureus bacterial proteins decreased in response to the time period of treating the bacterial cells with GBP. These results indicate that GBP could remarkably inhibit S. aureus and is, therefore, a potential food preservative.

Flow separation control of two kinds of microvortex generators for a supersonic spinning projectile

Jie Ma,Zhen-gui Huang,Zhihua Chen,Da-wen Xue,Jun-li Han 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.1

Previous studies have shown that Microvortex generators (MVGs) can effectively control the flow separation of supersonic boundary layer. To improve the flight stability of a spinning projectile, two kinds of MVGs, micro ramps and vanes, are applied to control the flow separation of an original standard 155 mm projectile, respectively. Numerical simulations were performed with the use of Detached eddy simulation (DES) method, and the flow fields of the projectile with and without micro MVGs were simulated, the characteristics of the boundary layer and aerodynamic data of three cases were compared and discussed. The numerical results reveal that the effect of the MVGs control is obvious, but their mechanisms are different, and the vanes are more effective than the ramp in the boundary layer control. However, they can both improve the lift and pitching moment of the projectile, and eliminate their oscillations; as a result, its flight stability and firing dispersion can be enhanced. Our results can provide important guidance for improving the flight quality of supersonic projectiles.

A mechanization method on the mixed operation of the numerical solutions, analytic solution, and showing their figures simultaneously for differential equation

Yu-Jie Ren,Fu-Gui Song,Yan-Juan Li,Hong-Qing Zhang 한국멀티미디어학회 2006 한국멀티미디어학회 국제학술대회 Vol.2006 No.-

In the paper, a new mechanization method on the mixed operation of the numerical solutions, analytic solution (if exist), and showing their figures simultaneously for differential equation and their MATLAB programs are presented. With the aid of computer software MATLAB, Euler's method is chosen to illustrate the validity and advantages of the method. As a result, we can not only see the analytic solution, numerical solutions, and their errors but also observe how step size affects the error of the numerical solutions by a figure. The mechanization method can be also applied to other numerical method.

A new compound operation method of the numerical solutions And analytic solution of partial differential equation

Yu-Jie Ren,Yan-Juan Li,Fu-Gui Song,Hong-Qing Zhang 한국멀티미디어학회 2006 한국멀티미디어학회 국제학술대회 Vol.2006 No.-

In the paper, a new compound operation method of the numerical solutions and analytic solution (if exist) is proposed to constructing explicit solutions for nonlinear partial differential equations based on computation system MATLAB. The validity and reliability of the method is tested by its application to the Toda-Lattice equations in (2+1) dimensions. More new explicit solutions are found. The compound operation method can be also applied to other nonlinear partial differential equations.