도축 한우의 복강내 지방괴사증 발생과 병리조직학적 연구

최길성 ( Gil Seong Choi ),임채웅 ( Chae Woong Lim ),채효석 ( Hyo Seok Chai ),최인열 ( In Yeol Choi ),이성효 ( Sung Hyo Lee ),김추철 ( Chu Cheul Kim ),서문정 ( Moon Jeong Suh ) 한국동물위생학회 1998 한국동물위생학회지 (KOJVS) Vol.21 No.1

The bovine fat necrosis is often seen as an incidental lesion in the adipose tissues of the abdominal cavity. Most of affected animals, however, have been detected at the slaughter house or routine necropsy. The purpose of this study was to examine the occurrence and distribution of abdominal fat necrosis in Korean native cattle, and its pathologic features. Postmortem inspection at an Chonju abattoir during a three-month period in 1997 detected at necrosis lesions in 67(6.6%) of the 1,012 animals received for slaughter. The occurrence was mainly in alimentary tracts and perirenal. Both sexes were affected, but the lesions were predominantly occurred in female. Gross lesions were white or yellowish in color and formed hard lumps irregular in shape ranging from small nodules to large solid masses. On the cut surface, lesions were occasionally seen chalky calcified granules and some of the lesions contained oil-like fluid. The surface of irregular shaped masses constricted kidneys and intestinal loops. Microscopically, the masses were encapsulated by thickened fibrous tissue, which infiltrated deeply and divided them into many irregular lobules. Initial lesions presented mild inflammatory cell and fibrous proliferation. It became fibroplasia in progressive lesions and resembled chronic lesion, eventhough no grossly apparent inflammatory reaction. The hard consistency of masses, due to fibroplasia, can compress the intestines, urinary organs, and reproductive organs.

The Rapid determination of Cycloxydim residue in agricultural products using QuEChERS and HPLC-MS/MS

Hyunjeong Cho,Chai Uk Lim,Hyo Young Kim,Min Ju Kim,Sun-gil Cho 한국분석과학회 2019 학술대회논문집 Vol.2019 No.11

국소 지혈제 사용이 갑상선 절제술 후 배액 양에 미치는 영향

김호찬 ( Ho Chan Kim ),임길채 ( Gil Chai Lim ),김상윤 ( Sang Yoon Kim ),남순열 ( Soon Yuhl Nam ),노종렬 ( Jong-lyel Roh ),최승호 ( Seung-ho Choi ) 대한갑상선학회 2008 International Journal of Thyroidology Vol.1 No.1

Background and Objectives: The amount of postoperative drainage is important factor to decide hospitalization and medical expense. The purpose of this study was to verify the advantage of using topical hemostatic agents after total thyroidectomy with central neck dissection (CND) to reduce postoperative drainage. Materials and Methods: From Jun 2007 to March 2008, 79 patients underwent total thyroidectomy with CND by one surgeon. Before wound closure, the operative bed was covered with fibrin sealant (group A, Beriplast^Ⓡ; n=22), microfibrillar collagen (group B, Avitene^Ⓡ; n=30) or nothing (group C, control; n=27). The amount of drainage was measured every 24 hours until drain removal. The drainage amount, hospital stay, and total medical expense were compared among the 3 groups by T-test. Results: The drainage amount for group A, B and C was 43.4 ml, 48.5 ml, 49.7 ml on the first postoperative day, 41.9 ml, 48.5 ml, 49.7 ml on the second day, 16.3 ml, 21.8 ml, 20.2 ml on the third day, respectively, resulting in no significant difference (p=0.08∼0.8). Hospital stay was not different among 3 groups. Total medical expense for group A,B and C was 2,602,118 Korean Won (KRW), 2,657,255 KRW, 2,496,647 KRW, respectively, which showed significantly lower medical expense in group C than group A or B. Conclusion: Topical hemostatic agents are not helpful to reduce the amount of the postoperative drainage and medical expense in cases of total thyroidectomy with CND.

아스피린 탈감작으로 호전된 아스피린 과민증을 동반한 난치성 만성 두드러기 2예

오민수 ( Min Su Oh ),성길명 ( Gil Myeong Seong ),이혜숙 ( Hye Sook Lee ),임길채 ( Gil Chai Lim ),이재천 ( Jae Chun Lee ) 대한천식알레르기학회 2012 천식 및 알레르기 Vol.32 No.1

Background: Chronic urticaria is a disease which is defined as hives with itching which waxed and waned for more than 6 weeks. In some cases, hives are intractable and refractory even while on medical treatment. Aspirin can cause and exacerbate chronic urticaria. We experienced two cases of intractable aspirin-sensitive chronic uriticaria which were successfully controlled by aspirin desensitization. Case History: Two adult patients had been suffering from hives with itching which were diagnosed as aspirin sensitive chronic urticaria. Their hives had worsened during medical treatment and had been exacerbated by aspirin administration. Results: Aspirin desensitization was attempted in each patient and the hives were successfully treated without having hives of daily administration of aspirin 300 mg. Conclusion: Aspirin desentization can be used as a treatment option in patients with chronic urticaria with aspirin sensitivity. (Korean J Asthma Allergy Clin Immunol 2012;32:51-55)

Changes of Serum Aldosterone Concentration after Noise Exposure in Mice

Jong Woo Chung,Hyung Min Song,Gil Chai Lim,임현우,Min Ju Kim,Seung Hyo Choi 대한청각학회 2011 Journal of Audiology & Otology Vol.15 No.3

Introduction Aldosterone is a mineralocorticoid (MC) that is released from the zona glomerulosa of the adrenal cortex. Several recent studies have shown the stria vascularis has numerous MC and glucocorticoid receptors.1,2) The MC receptor of the inner ear controls the homeostasis of the endolymph by means of ion channels and transporters that exist in the cochlear duct cells.3,4) Deprivation or imbalance of such activities affects the morphological functions of the related cells, which leads to hearing loss.5,6,7)Even though little animal research is available on the relevance of hearing loss and aldosterone, it has been reported that injection of aldosterone to mrl/mpj-fas (lpr) autoimmune mice that have stria vascularis and no hair cell pathology facilitates the regeneration of the stria vascularis to its normal form.8) Since stria vascularis abnormality causes abnormal sodium ion (Na+) and potassium ion (K+) transport, it can be suggested that the enhancement of such transport is necessary to fully recover normal hearing level.8) Recently, it has also been reported that the difference in serum aldosterone concentration between normal hearing and presbycusic groups is highly significant.9) Although there are no explanations available, the decreased serum aldosterone concentration, which affects the expression of Na, K-ATPase and Na-K-Cl cotransporter (NKCC1) on the cell membranes of the inner ear cells, is related to the disrupted homeostasis of endolymph due to abnormal K+ regeneration in the cochlea.9)Aldosterone plays a critical role in maintaining the homeostasis of the inner ear. However, previous studies have tended to focus on noise-induced hearing loss and steroid injection.10,11) Not much is known about the protein changes related to K+ regeneration and the role(s) of aldosterone in noise-induced hearing loss. The aim of this study was to determine whether serum aldosterone is influenced by noise exposure by comparing aldosterone levels of BALB/c mouse before and after noise exposure. Materials and Methods Experimental animals Nine, 6-week-old, male BALB/c mice (Orient Co., Seongnam, Korea) weighing 21-24 g with normal Preyer's reflexes and normal hearing at auditory brain stem response were used. The mice were randomly divided into three groups (n=3 per group; see below), weighed, and bred in a cage. All mice were allowed unrestricted access to food and water. Organization of experimental groups Serum aldosterone was measured in each of the three groups. In the control group, mice were not exposed to noise. Another group was noise-exposed for 3 consecutive days prior to measurement of serum aldosterone immediately after termination of the exposure (0 day after noise group). The last group was also noise-exposed for 3 days but serum aldosterone was measured 3 days after termination of the exposure (3 days after group). Instruments for noise exposure For noise exposure, a sound-proof noise booth was used. Noise was generated by a speaker with input/output resistance of 8 Ω (290-8L; Altec Lansing, Oklahoma City, OK, USA) that was directly amplified through a model R-399 amplifier (INTER M, Seoul, Korea). The amplifier was placed in the left corner of the room and the speaker was placed above the amplifier, with the speaker horn at a 45° angle. Noise-induced hearing loss Noise-exposed mice were rounded up in a cage, placed inside a noise booth, and exposed to white noise of 120 dB peak equivalent SPL for 3 hours per day for 3 days to induce permanent threshold shift (PTS). Noise was generated through Cool Edit 1.52 PC software, and the amplitude of the noise was measured over 120 dB SPL using a sound level meter (B&K, Nærum, Denmark) in the center and each corner of the soundproof room. The amount of noise exposure used in this study to cause PTS has been previously demonstrated to induce continuous threshold change in a study that used the same equipment and conditions.12) Measurement of hearing... Background and Objectives: The study was designed to measure the change of serum aldosterone concentration after noise exposure in BALB/c mice. Materials and Methods: BALB/c hybrid mice with ≤25 dB nHL in auditory brainstem responses (ABR) were used. Six mice were exposed to 120 dB SPL broad white band noise for 3 hours per day for 3 consecutive days. ABRs in all mice were examined after noise exposure. Serum aldosterone concentration was checked by radioimmunoassay in three mice without noise exposure, in three mice immediately after noise exposure (0 day after noise group), and in three mice 3 days after noise exposure (3 days after noise group). Results: Permanent noise exposure-related threshold shift was induced in mice of 0 day after noise group and 3 days after noise group (83.3±2.9 and 88.3±2.9 dB nHL, respectively). The serum aldosterone concentration of the mice after noise exposure was significantly lower than control mice (p=0.046). The changes of aldosterone concentration were 879±137.5 pg/mL without noise exposure, 623±75.9 pg/mL immediately after noise exposure, and 683±49.2 pg/mL 3 days after noise exposure. Conclusions: In BALB/c mice, serum aldosterone concentration is decreased significantly after noise exposure

제주 지역 간호사의 구강 악안면 영역 손상에 대한 응급 처치 인식도

이병진(Byoung-Jin Lee),송효정(Hyo-Jeong Song),임길채(Gil-Chai Lim),감세훈(Se-Hoon Kahm),김성준(Sung-Joon Kim) 대한치과의사협회 2012 대한치과의사협회지 Vol.50 No.12

The purposes of this work were to assess the cognition of the registered nurse(RN) on oral and maxillofacial emergency treatment and to compare cognition of the RN with that of the 119 emergency medical technician(EMT). 450 RNs who were working at each of secondary hospitals in Jeju province had responded to the questionnaire. Independent sample t-test and chi-square test were used to assess the state of RN on dental emergency treatment and to compare RN with EMT. The question "education time on dentistry in formal education" that marked "0 hour" and "1-3 hours" were 73.3% and 20.0%, respectively. The question "refresher training class on dentistry" that marked "0 hour" and "1-3 hours" were 92.9% and 6.7%, aggregately 99.6%. The results showed low score in the question "reduction of temporo-mandibular joint(TMJ)"(1.67±0.857), "fixation of dislocated TMJ"(1.70±0.853) and "post-avulsed tooth treatment"(1.78±0.774) by 5-point Likert scale. Likewise, the scores were 2.02±0.806 in the question "treatment of maxillofacial trauma", 2.76±1.061 in the question "emergent care of avulsed tooth", 2.70±1.095 in the question "treatment time of avulsed tooth" and 2.79±1.056 in the question "mouth guard", respectively. Compared to EMT, results of RN showed a statistically lower figure(ph0.05) in all items compared except the question "medicine control", and the question "doctor care in emergency room" was borderline(p=0.069). From this study, it is necessary for RN and student of nursing science to be educated on the oral and maxillofacial emergency treatment for the initial management of injuries. Authors suggest further co-study and nation-wide research with nursing care.

갑상선 역형성 암종의 예후 인자에 관한 연구

허성철,김상윤,남순열,임길채,유승주 대한이비인후과학회 2001 대한이비인후과학회지 두경부외과학 Vol.44 No.10

Proteomic Analysis of the Protein Expression in the Cochlea of Noise-Exposed Mice

Jong Woo Chung,Nam Kyung Yeo,안윤숙,Ji Won Kim,Seung Hyo Choi,Gil Chai Lim 대한청각학회 2011 Journal of Audiology & Otology Vol.15 No.3

Background and Objectives: When noise-induced hearing loss occurs, destruction of the hair cells is accompanied by mechanical injury, chemical injury, and hypoxia. Proteomics is a powerful tool for protein analysis, as it provides valuable information regarding the biochemical processes involved in diseases, monitors cellular processes, and characterizes protein expression levels. We attempted to identify the proteins associated with the pathophysiology of noise-induced hearing loss, as well as the mechanisms of this disease, using a proteomics approach. Materials and Methods: We used BALB/C male mice. The control mice were placed in a booth without noise, while the experimental mice were exposed to noise for three hours daily for three consecutive days. Cochleae from each group were obtained for total protein extraction. The proteins were separated into numerous spots using two-dimensional electrophoresis. Seven protein spots that were strongly detected only in the noise-exposed cochleae were selected and subsequently analyzed using matrix-assisted laser desorption/ionization time of flight mass spectrometry. Results: Approximately 286 protein spots were detected in the noise group. Seven selected spots were analyzed and various proteins identified, including tyrosine protein kinase MEG2, angiopoietin-like 1, heat shock 70 kDa protein, sodium dicarboxylate cotransporter 1, myeloid Elf-1-like factor, disintegrin, metalloproteinase domain 7, and activated leukocyte-cell adhesion molecule. Conclusions: We identified several proteins expressed in noise-induced hearing loss using a proteomics approach. These proteins may help us to understand the pathogenic mechanisms of noise-induced hearing loss. Introduction Noise is a common cause of sensorineural hearing impairment in industrialized countries. Millions of people currently have disabilities caused by noise-induced hearing loss and experience problems in communication with families, colleagues, and friends.1) In particular, tinnitus is one of the symptoms of noise-induced hearing loss that brings severe social isolation and leads to degraded quality of life. Noise exposure can physically destroy the tympanic membrane, middle ear, and inner ear and can alter the intracellular pathways that lead to cell necrosis or apoptosis, and induced hearing loss worsens. In addition, noise stimulation was recently found to induce metabolic changes inside the inner ear. Possible mechanisms underlying this noise-induced tissue damage are oxidative stress and the reduction of cochlear blood flow.2) Studies have been conducted in the last few decades on mechanical trauma and the metabolic damage of mechanical changes. Vascular endothelial growth factor, nuclear factor κB, glucose transporter-1, and hypoxia-inducible factor-1α are thought to be causes of noise-induced hearing loss. Most of them were histologically confirmed by immunohistochemical and fluorescence staining.4,5,6)Proteomics is a powerful tool for protein analysis, as it provides valuable information regarding the biochemical processes involved in diseases, monitors cellular processes, and characterizes protein expression levels. We can understand the pathophysiology of disease through analysis of the proteins involved using proteomics technology, which includes two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). These techniques can be used to produce a high-resolution two-dimensional map, in which individual (stained) proteins appear as spots of various sizes and intensities that depend on the amount of each protein in the sample.7,8)To understand the proteins associated with the pathophysiology of noise-induced hearing loss, as well as the mechanisms of the disease, we compared expressed proteins in noise-exposed mice with those in unexposed mice using this proteomics approach. For this, we investigated expressed protein spots only in noise-exposed mice. Materials and Methods Materials As experimental groups, 8-week-old BALB/c mice with normal Preyer's reflexes and normal hearing thresholds in auditory brainstem response were studied. Methods Experimental setting All mice were placed in separate soundproof booths with blocks to outside noise. We set an amplifier (R-399, INTER M, Seoul, Korea) in the left side of the room and placed an 8 Ω resistance speaker (290-8L, ALTEC LANSING, Oklahoma City, OK, USA) on the amplifier with a 45 degree horn. Anesthesia of experimental groups All mice were anesthetized (Ketamine hydrochloride 59 mg/kg and xylazine 1.3 mg/kg body weight) via intraperitoneal injection. If necessary, we injected an additional half dose of anesthestics. Threshold measurement The auditory brainstem response (ABR) to click stimuli was recorded, and thresholds were obtained for each ear. Hearing thresholds are measured at wave I lowering by 10 dB from an intensity of 90 dB hearing level (HL). When the wave was not definite, we checked threshold lowering by 5 dB. Click stimuli were filtered from 100 to 3,000 Hz, and the frequency was 1,024/min. Noise exposure procedure Ten mice were exposed continuously for 3 h/day to a 120 dB sound pressure level (SPL) broad band click sound for 1-5 consecutive days. In the noise booth, mice were randomly divided into two groups, and their location inside the booth was changed daily so that each animal was exposed to the same level of noise. Ten normal BALB/c mice kept for 3 h/day for 5 consecutive days in the same noise booth without noise were used as the control group. Acoustic trauma was induced by a continuous pure tone of 6 kHz generate...

성대용종 환자의 음성치료 효과

김성태(Kim Seong-Tae),정고은(Jeong Go Eun),김상윤(Kim Sang Yoon),최승호(Choi Seung-Ho),임길채(Lim Gil Chai),한주희(Han Ju Hee),남순열(Nam Soon Yuhl) 한국음성학회 2009 말소리와 음성과학 Vol.1 No.2

거주환경의 차이가 제주지역 소아의 흡입 알레르겐에 대한 감작률 및 알레르기질환 유병률에 미치는 영향

이혜숙 ( Hye Sook Lee ),홍성철 ( Sung Chul Hong ),김수영 ( Su Young Kim ),이근화 ( Keun Hwa Lee ),김재왕 ( Jae Wang Kim ),김정홍 ( Jeong Hong Kim ),최승효 ( Seung Hyo Choi ),임길채 ( Gil Chai Lim ),이재천 ( Jae Chun Lee ),신경수 ( 대한소아알레르기호흡기학회(구 대한소아알레르기 및 호흡기학회) 2011 소아알레르기 및 호흡기학회지 Vol.21 No.3

Purpose: This study is to investigate the influence of the residential environment on the sensitization rates to aeroallergens and the prevalences of atopic disorders in the school children. Methods: Two elementary schools in Jeju, Korea were selected according to their distinctive residential environment, one located in the area surrounded by the tangerine farms and Japanese cedar forests (tangerine farming community) and the other rarely with them (non-tangerine farming community). All the school children (1,550 students) from the two school were enrolled in this study. Under their parents` informed consent, surveys based on International Study of Asthma and Allergies in Childhood questionnaire were answered by the parents and skin prick tests with 16 common aeroallergens were performed. Results: The questionnaire is responded from 1,290 parents (83.2%) and the skin test was done in 1,284 students (82.8%). The sensitization rates to more than one aeroallergen was 41.9%, increasing by age. The children residing in the tangerine farming community showed significantly higher sensitization rates than those from non-farming one (47.5% vs. 38.4%, P=0.004). The former were sensitized more frequently to house dust mite, citrus red mite and Japanese cedar pollen, while the others to outdoor moulds. However, the prevalence of atopic disorders had no difference between the two groups. Conclusion: The residential environment might influence the sensitization rates to prevalent aeroallergens in the environment among school children, but there is no difference in the prevalence of the atopic disorders. [Pediatr Allergy Respir Dis(Korea) 2011;21:176-185]